Caco 2 cells. Medical search. Frequent questions (2)

However, the apparent permeability coefficients of the test drugs across Caco-2 monolayers measured by the sample pooling or cassette dosing (up to five drugs co-administered in this study) strategy were in good conformity with the data obtained by single-drug dosing followed by discrete sample analysis. (nih.gov)
To further characterize cytochrome P450 (CYP) and P-glycoprotein (Pgp) expression in monolayers of the Caco-2 cell clone TC7, a cell culture model of the human intestinal epithelium. (nih.gov)
mRNA expression of eight CYPs and Pgp was investigated in TC7 and parental Caco-2 (Caco-2p) cell monolayers using RT-PCR. (nih.gov)
Cell monolayers were exposed to alginates with various mannuronic to guluronic acid ratios at three different concentrations, and cellular ferritin was measured as a surrogate marker of iron uptake into the cell. (uea.ac.uk)
A correlation between the in vitro apparent permeability across Caco-2 monolayers and the in vivo fraction absorbed has been reported. (wikipedia.org)
The aim of this study was thus to determine the effect of nicotine, the major constituent in cigarettes and its metabolites on the integrity of tight junctions in Caco-2 cell monolayers. (ulster.ac.uk)
C. jejuni caused no changes in short-circuit current upon infection of Caco-2 cell monolayers in Ussing chambers. (brighton.ac.uk)
C. jejuni infection of fluid-transporting ('doming') Caco-2 cells resulted in a significant reduction in dome number, which correlated with a decrease in tight junction integrity in infected monolayers, when measured as transepithelial electrical resistance. (brighton.ac.uk)
C. jejuni also altered the distribution of the tight junction protein occludin within cell monolayers. (brighton.ac.uk)
Thus, tight junction integrity is required for fluid transport in Caco-2 cell monolayers as leaky tight junctions cannot maintain support of transported fluid at the basolateral surface of infected cell monolayers. (brighton.ac.uk)
To evaluate virus-like contamination, infections (2 PFU/cell) in presenting stream (MEM made up of 20 mM HEPES) had been incubated with polarized monolayers for 1 h at 4C. (palomid529.com)
1 Monolayers of Caco‐2 cells, a transformed cell line derived from human colorectal carcinoma, have been widely used by pharmaceutical companies as a standard intestinal barrier model for the prediction of intestinal permeability for drug candidates. (sigmaaldrich.com)
The cell monolayers also displayed tight junctions and electrical resistance. (cdc.gov)

The Caco-2 cell monolayer model is widely used as a standard screening tool for studying the mechanisms of cellular drug transport. (nel.edu)
Caffeine transport (0.1, 0.3, 1 and 10 mmol/l) was studied in Caco-2 cell monolayer in apical to basolateral (AP-BL) and basolateral to apical (BL-AP) direction, under iso-pH 7.4 and pH-gradient (6/7.4) conditions. (nel.edu)
Caco-2 cells may be used as a confluent monolayer on a cell culture insert filter (e.g. (wikipedia.org)
In this format, Caco-2 cells form a polarized epithelial cell monolayer that provides a physical and biochemical barrier to the passage of ions and small molecules. (wikipedia.org)
The Caco-2 monolayer can be used as an in vitro model of the human small intestinal mucosa to predict the absorption of orally administered drugs. (wikipedia.org)
The Caco-2 cell monolayer model is a popular surrogate in predicting the in vitro human intestinal permeability of a drug due to its morphological and functional similarity with human enterocytes. (edu.hk)
As intestinal permeability is a key parameter of oral toxicokinetics, the apparent permeability of MC-RR across a differentiated intestinal Caco-2 cell monolayer was investigated. (hal.science)
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
Nonheme eiron bioavailability was assessed by Caco-2 cell monolayer 59Fe uptake using an extrinsic labeling method. (usda.gov)

In this study, the effects of intracellular delivery of various concentrations of gallic acid (GA) as a semistable antioxidant, gallic acid-loaded mesoporous silica nanoparticles (MSNs-GA), and cellular uptake of nanoparticles into Caco-2 cells were investigated. (springer.com)
TEM and fluorescent microscopy confirmed the internalization of MSNs by Caco-2 cells through nonspecific cellular uptake. (springer.com)
The cell viability of Caco-2 cells was affected during MSNs-GA uptake. (springer.com)
Cauda V, Engelke H, Sauer A, Arcizet D, Brauchle C, Radler J, Bein T (2010) Colchicine-loaded lipid bilayer-coated 50 nm mesoporous nanoparticles efficiently induce microtubule depolymerization upon cell uptake. (springer.com)
Chung TH, Wu SH, Yao M, Lu CW, Lin YS, Hung Y, Mou CY, Chen YC, Huang DM (2007) The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles 3T3-L1 cells and human mesenchymal stem cells. (springer.com)
To this aim, we supplied [U-(13)C(6)]glucose to Caco-2 cells infected with the bacterial strains or mutants thereof impaired in the uptake of glucose, mannose and/or glucose 6-phosphate. (uni-muenchen.de)
Mutants of all three strains impaired in the uptake of glucose switched to C(3)-substrate(s) accompanied by an increased uptake of amino acids (and possibly also other anabolic monomers) from the host cell. (uni-muenchen.de)
Polarized caco-2 cells express tight junctions, microvilli, and a number of enzymes and transporters that are characteristic of such enterocytes: peptidases, esterases, P-glycoprotein, uptake transporters for amino acids, bile acids, carboxylic acids, etc. (wikipedia.org)
The aim of the project was to identify if transporters are involved in cellular uptake of vigabatrin in Caco-2 cells. (ku.dk)
Methods: The uptake rate of vigabatrin was measured in Caco-2 cells at pH 6.0 or 7.4 for 15 min after application of 0.1 - 25.0 mM vigabatrin. (ku.dk)
The inhibitory effect of selected amino acids and -derivatives on the apical vigabatrin uptake in Caco-2 cells was investigated. (ku.dk)
Results: The uptake rate of vigabatrin in Caco-2 cells was pH-dependent. (ku.dk)
The presences of hPAT1 ligands significantly inhibited the uptake of vigabatrin in Caco-2 cells at pH 6.0, whereas hPAT1 non-ligands did not. (ku.dk)
The uptake of [ 3 H]vinblastine, a substrate of P-gp, by Caco-2 cells was measured. (aspetjournals.org)
No significant effect on the uptake of 3- O -[ 3 H]methylglucose or [ 14 C]phenylalanine by Caco-2 cells was found, compared with the control. (aspetjournals.org)
The ethyl acetate extract of orange juice and these methoxyflavones also increased steady-state [ 3 H]vinblastine uptake by LLC-GA5-COL300 cells (a cell line transfected with human MDR1 cDNA). (aspetjournals.org)
Poor relationship between Caco-2 permeability and log Ko/w r2 = 0.097 THESE THEORIES OF DRUG UPTAKE WERE BIOPHYSICAL, 'LIPID-ONLY' THEORIES Corti et al. (slideshare.net)
Dye-uptake technique, in which neutral red dye is incorporated into viable cells, was used to assess the cytotoxicity of the accessories. (bvsalud.org)

Transwell diagram Cell culture Drug development Pre-clinical development PAMPA - a non cell-based permeability assay Lea, Tor (2015). (wikipedia.org)
Cell viability was measured using the MTT assay. (hindawi.com)

Although derived from a colon (large intestine) carcinoma, when cultured under specific conditions the cells become differentiated and polarized such that their phenotype, morphologically and functionally, resembles the enterocytes lining the small intestine. (wikipedia.org)
Microscopically, Caco-2 cell cultures show obvious heterogeneity likely reflecting the complex mixture of cells found in the epithelial lining of the large and small intestine i.e. enterocytes, enteroendocrine cells, goblet cells, transit amplifying cells, paneth cells and intestinal stem cells. (wikipedia.org)
The purpose of this study was to determine whether chronic stimulation of intestinal mucosa by interferon-γ (IFN-γ) affects expression and function of the apical membrane Na + /H + exchangers NHE2 and NHE3 in rat intestine and Caco-2/bbe (C2) cells. (elsevierpure.com)

However, irrespective of the loaded concentration and exposure time, the permeabilities were very low from apical to basolateral compartments (from 4 to 15 × 10−8 cm·s−1) and from basolateral to apical compartments (from 2 to 37 × 10−8 cm·s−1). (hal.science)
Genetic deletion of Dnmt3a in intestinal epithelial cells ( Dnmt3a ΔIEC ) in mice confirms the phenotype of an altered epithelial ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in the colon in vivo. (nature.com)
Cells displayed many of the properties that characterize enterocytes, such as apical microvilli, basolateral basement membrane, and glycogen. (cdc.gov)

Human colon adenocarcinoma (Caco-2) and human embryonic kidney (HEK-293) cells were maintained and treated with various concentrations (0, 20, 40, 80, 100 and 120 mg/l) of K. africana, M. zehyeri and X. caffra seed oil. (iiarjournals.org)
We therefore undertook a preliminary screening study of the three IFBTS seed oils in vitro using human colon adenocarcinoma cells (Caco-2), a commonly used gastrointestinal cell line, and human embryonic kidney (HEK-293), an epithelial immortalised cell line. (iiarjournals.org)
These findings provide new molecular basis for further understanding the mechanisms involved in ATP functions, as a signal transducer and activator of MAP kinase cascades, in Caco-2 cells derived from human colon adenocarcinoma. (conicet.gov.ar)

MSNs can easily internalize into Caco-2 cells without deleterious effects on cell viability. (springer.com)
Cell viability of five compounds were tested: two compounds (flavopiridol and loperamide) had negative effects on cell viability, three compounds (paracetamol, ketoprofen, alosetron) had no effects on cell viability. (sigmaaldrich.com)

For MSNs-GA, cell viability was reduced as a function of incubation time and different concentrations of nanoparticles. (springer.com)
Dispense 100 µL/well CellTiter-Glo ® 3D Cell Viability Reagent. (sigmaaldrich.com)
Cytotoxic effects of five compounds (flavopiridol, loperamide, paracetamol, ketoprofen and alosetron) on human colon organoids using the CellTiter-Glo ® 3D Cell Viability Reagent. (sigmaaldrich.com)
Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)

Surprisingly, the metabolism of the host cells, as judged by the efficiency of (13)C-incorporation into host cell amino acids, was not significantly affected by the infection with either of these intracellular pathogens. (uni-muenchen.de)
K. africana oil significantly suppressed Caco-2 cell growth compared to HEK-293 cell growth at all oil concentrations, whereas M. zeyheri and X. caffra seed oils significantly suppressed HEK-293 and Caco-2 cell growth, only at a concentration of 80 mg/l. (iiarjournals.org)
Ferritin concentrations were significantly higher when the cells were treated with ferric ammonium citrate and 0.5 and 1% w/v (but not 0.1%) alginate, but were unaffected by mannuronic/guluronic acid ratios. (uea.ac.uk)
Moreover, the presence of MFGM in the dairy matrix was found to decrease significantly the bacterial attachment ability to Caco-2 TC7 cells. (uantwerpen.be)
Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
Finally, an exploratory, observational, pilot study involving 36 infants diagnosed with at least one FGID (infant colic and/or functional constipation) showed the probiotic formula was well tolerated and FGID severity was significantly reduced after 14 days of treatment with the 2 strains. (frontiersin.org)

In this study, we determined the effects of the Kigelia africana, the Mimusops zeyheri and the Ximenia caffra seed-oil on cell proliferation in culture. (iiarjournals.org)
The suppression of Caco-2 and HEK-293 cell proliferation by K. africana, M. zeyheri and X. caffra seed oils suggest a potential antiproliferative effect of these tree seed oils on the two cell lines. (iiarjournals.org)
The objective of this study was to determine the effect of K. africana, M. zeyheri and X. caffra seed oils on Caco-2 and HEK-293 cell proliferation. (iiarjournals.org)
And the influence of FASN on proliferation and migration of colorectal cancer cells was also explored. (biomedcentral.com)
However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)
In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)
8000 Bahía Blanca, Argentina In the present work, we examined the role of ATP in the activation of the MAP kinases (MAPKs) ERK1/2, JNK1/2 and p38 and their involvement in the modulation of transcription factors and proliferation of human colon cancer Caco-2 cells. (conicet.gov.ar)
We demonstrate, by confocal microscopy, that stimulation of Caco-2 cells with ATP results in the translocation of active MAPKs to the nucleous where they induce the expression of c-Fos and Jun family proteins, the phosphorylation of ATF-1, ATF-2 and JunD transcription factors and stimulate Caco-2 cell proliferation. (conicet.gov.ar)
Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)

Caco-2 cells and Biopharmaceutics Classification System (BCS) for prediction of transepithelial transport of xenobiotics (model drug: caffeine). (nel.edu)
Smetanova I, Stetinova E, Kholova A, Kvetina A, Smetana A, Svoboda B, Caco-2 cells and Biopharmaceutics Classification System (BCS) for prediction of transepithelial transport of xenobiotics (model drug: caffeine). (nel.edu)
The integrity of Caco-2 tight junctions was analysed by measuring the transepithelial electrical resistance (TER) and by tracing the flux of the fluorescent marker fluorescein, after treatment with various concentrations of nicotine or nicotine metabolites over 48 h. (ulster.ac.uk)
Conjugated linoleic acid (CLA) exhibits isomer-specific effects on transepithelial calcium (Ca) transport as well as on cell growth in human intestinal-like Caco-2 cells. (uea.ac.uk)
The asymmetric distribution of the Na,K-ATPase in the plasma membrane of epithelial cells is essential to the establishment of the transepithelial Na + gradient that supports the vectorial transport of ions and solutes. (wustl.edu)
Ablation of DNMT3A in Caco-2 cells results in global DNA hypomethylation, which is linked to impaired regenerative capacity, transepithelial resistance and intercellular junction formation. (nature.com)

Global gene expression analysis reveals differences in cellular responses to hydroxyl- and superoxide anion radical-induced oxidative stress in caco-2 cells. (bvsalud.org)

Mechanisms of toxicity include inhibition of enzyme activity and protein synthesis, alterations in nucleic acid function, and changes in cell membrane permeability. (medscape.com)

Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells. (uni-muenchen.de)
In this study, we analysed the intracellular carbon metabolism of enteroinvasive Escherichia coli (EIEC HN280 and EIEC 4608-58) and Salmonella enterica Serovar Typhimurium (Stm 14028) replicating in epithelial colorectal adenocarcinoma cells (Caco-2). (uni-muenchen.de)
Caco-2 (from Cancer coli, "colon cancer") is an immortalized cell line of human colorectal adenocarcinoma cells. (wikipedia.org)

Everest, Paul H. / Campylobacter jejuni inhibits the absorptive transport functions of Caco-2 cells and disrupts cellular tight junctions . (brighton.ac.uk)

MSNs did not show significant toxicity in various concentrations (0-500 μg/ml) on Caco-2 cells. (springer.com)
Toxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. (aber.ac.uk)
Here we demonstrate the use of highly-characterized human iPS cell-derived colon organoids as an alternative cell model for traditional Caco-2 cell drug toxicity assays for drug screening applications. (sigmaaldrich.com)

Despite such heterogeneity, Caco-2 cells are used in cell invasion studies, viral transfection research, and lipid transport. (wikipedia.org)
Transfection of Caco-2 cells with siRNAs (20 nM focus) was performed as explained previously (4). (palomid529.com)

The two strains mediate adhesion by different mechanisms and, when combined, synergically induce the expression of Caco-2 tight junction proteins. (frontiersin.org)

The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)

Poor correlation between Caco-2 cells and artificial membrane (PAMPA) assays Note axis scales Balimane et al. (slideshare.net)
Transmission electron microscopy and quantitative cell based assays were used in exploring point (c). (lu.se)

Caco-2 cells are models of absorptive enterocytes. (brighton.ac.uk)
Inhibition of absorptive cell function, changes in epithelial resistance and rearrangement of tight junctional proteins such as occludin represent a potential diarrhoeal mechanism of C. jejuni. (brighton.ac.uk)

Animal-derived oils and purified fatty acids, but not indigenous fruit-tree-derived seed oils, have been used to study cell growth and differentiation. (iiarjournals.org)

In this study, the bioavailability of lutein from chlorella during chlorella digestion was evaluated by using the coupled in vitro digestion and human intestinal Caco-2 cell model. (kist.re.kr)

A method for high-throughput Caco-2 permeability screening of drug candidates has been developed using thirteen generic drugs as test compounds. (nih.gov)

The patient is further monitored for IBD, especially because preliminary experiments showed a Caco-2 cell cycle arrest after incubation with the H. trogontum isolate and this effect may be involved in the early stage of IBD. (cdc.gov)
Adenine has been suggested to induce cell cycle arrest in cancer cells, leading to cell death [ 9 , 10 ]. (hindawi.com)
Next to gene expression , correlations with related phenotypic markers ( 8-oxodG levels and cell cycle arrest ) were investigated. (bvsalud.org)

The cytotoxicity of MSNs and MSNs-GA at low and high concentrations were studied by means of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test and flow cytometry. (springer.com)
Results showed that Caco-2 TC7 cells incubated long-term with cadmium concentrations ranging from 0 to 10 micromol Cd/l for 5 weeks exhibited a significant increase in cadmium accumulation. (unboundmedicine.com)
CO 2 concentrations can be finely controlled by alveolar ventilation, and H + and HCO 3 − concentrations can be finely regulated by renal excretion. (msdmanuals.com)

Biological characterization of oils using of cells in culture has largely focused on animal-derived oils and purified fatty acids while little if any investigations have been carried out on seed oils from IFBTs. (iiarjournals.org)
Caco-2 cells with a high expression of both HER2 and FASN were selected for functional characterization. (biomedcentral.com)
In vitro characterization revealed that the strains tolerate gastric and bile challenges and display a great adhesion capacity to human intestinal cells. (frontiersin.org)

Compared with the reported QSAR/QSPR models about Caco-2 cell permeability, our model exhibits certain advantage in database size and prediction accuracy to some extent. (edu.hk)

American Journal of Physiology - Cell Physiology , 280 (5 49-5), C1224-C1232. (elsevierpure.com)
2 Despite their convenience and accessibility, Caco-2 cells have a single homogeneous phenotype, and therefore fail to recapitulate lineage development into the diverse cell phenotypes that constitute the physiology of the native intestinal epithelium, leading to inconclusive experimental results. (sigmaaldrich.com)

abstract = "Ethnopharmacology relevanceMaytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.Aim of the studyWe investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.Materials and MethodsToxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. (aber.ac.uk)

In this work, we analyze expression patterns of DNMT3A in patients with IBD, perform loss of function experiments using Caco-2 cell line and generate a conditional Dnmt3a ΔIEC mouse line. (nature.com)
Caco-2 cells were exposed to 50 µg/ml MWCNT (oxidized or pristine) for 24 h, and experiments were repeated in the presence of 2.5 mg/L natural organic matter. (cdc.gov)

We present proof that autophagy-related gene items are essential for EV7 access into polarized Caco-2 cells. (palomid529.com)
Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge. (aber.ac.uk)
Therefore, we studied global gene expression by an extensive time series (0.08, 0.25, 0.5, 1, 2, 4, 8, 16, or 24 h) analyses in human colon cancer (caco-2) cells after exposure to H(2)O(2) or the superoxide anion donor menadione . (bvsalud.org)
Differences in gene expression were investigated by hybridization on two- color microarrays against nonexposed time -matched control cells . (bvsalud.org)
Gene expression analysis resulted in 1404 differentially expressed genes upon H(2)O(2) challenge and 979 genes after menadione treatment . (bvsalud.org)

A downregulation of FASN effectively inhibits the activity of "HER2-PI3K/Akt axis" and alters the malignant phenotype in colorectal cancer cells. (biomedcentral.com)
Hence, in this study, we analyzed the potential link between FASN and the activity of "HER2-PI3K/Akt axis" in colorectal cancer cells. (biomedcentral.com)
It is of interest to explore the inhibitory effect of adenine on the growth of colorectal cancer cells and to determine the underlying mechanism. (hindawi.com)
We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)

Chronic exposure of intestinal epithelial cells to IFN-γresults in selective downregulation of NHE2 and NHE3 expression and activity, a potential cause of inflammation-associated diarrhea. (elsevierpure.com)
Usually, the response involves inflammation, which dilates vessels and creates blood flow while also recruiting white blood cells (WBCs) to fight off the foreign invaders. (bodyecology.com)

The potential use of alginates as a vehicle for water-soluble (bioavailable) iron for fortifying food products was examined using a Caco-2 cell model system. (uea.ac.uk)
The best Boosting model was obtained finally with R 2 = 0.97, RMSE F = 0.12, Q 2 = 0.83, RMSE CV = 0.31 for the training set and R T 2 = 0.81, RMSE T = 0.31 for the test set. (edu.hk)
We investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway. (aber.ac.uk)
The aim of this study was to determine whether multiwalled carbon nanotube s (MWNCT) are taken up by and are toxic to human intestinal enterocytes using the Caco-2 cell model. (cdc.gov)

This work thus demonstrated a possible competition between LGG adhesion to MFGM and to epithelial intestinal cells. (uantwerpen.be)
This competition could negatively impact the adhesion capacity of LGG to intestinal cells in vivo, but requires further substantiation. (uantwerpen.be)

The (13)C-isotopologue patterns of protein-derived amino acids from the bacteria and the host cells were then determined by mass spectrometry. (uni-muenchen.de)
Interestingly, α1 and β1 message increases in MDCK cells with the development of polarity, yet there is little change in the amount of protein for either subunit. (wustl.edu)
PI3K activity was thought to be the mediator of the HER2 control on FASN expression because LY294002, a known PI3K inhibitor, abrogated HER2 induced FASN protein production in the HER2 overexpressing normal mammary epithelial and breast cancer cells. (biomedcentral.com)
Remarkably, at least one of these protein, Atg16L1, is usually essential for computer virus internalization from the cell surface area rather than for intracellular trafficking. (palomid529.com)
The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)

These results demonstrate that the regulation of Na,K-ATPase α1 and β1 isoform expression is mediated by a combination of transcriptional and translational events during the development of polarity in both cell lines. (wustl.edu)

Caco-2 cells are known to form tight junctions ( 13 - 14 ), have structural properties similar to those of ileal enterocytes ( 15 ) and are hence useful models as they mimic the gastrointestinal tract. (iiarjournals.org)
Our study suggests that intestinal enterocytes cells do not take up MWCNT. (cdc.gov)

All three tree seed oils suppressed both Caco-2 and HEK-293 cell growth in a dose-dependent manner. (iiarjournals.org)

The HEK-293 cell line has been used extensively ( 16 - 17 ) as a laboratory work-horse in several cell culture studies. (iiarjournals.org)
In this study, the authors used DNA microarray to compare and identify genes induced by HER2 in mammary epithelial cell line with ectopic HER2 overexpression and breast cancer cell lines derived from patients with different level of HER2 expression. (biomedcentral.com)
RT-PCR studies and PCR product sequencing supported the expression of P2Y2 and P2Y4 receptors in this cell line. (conicet.gov.ar)
The epidermal growth factor receptor (EGFR) specific inhibitor AG1478 decreased ERK1/2, JNK1/2 and p38 MAPK phosphorylation by ATP, suggesting that EGFR transactivation is important for ATP-mediated stimulation of MAPKs in this intestinal cell line. (conicet.gov.ar)

Our study was conducted 1) to characterize the mechanisms of caffeine transport across the intestinal barrier, 2) to classify caffeine according to BCS, 3) to predict drugs intestinal absorption in humans. (nel.edu)

Metabolism of carbohydrates and fats generates 15,000 to 20,000 mmol of carbon dioxide (CO 2 ) daily. (msdmanuals.com)

DNMT3A expression is downregulated in intestinal epithelial cells from IBD patients and upon tumor necrosis factor treatment in murine intestinal organoids. (nature.com)
Stem cell- and tissue- derived organoids have more complex 3D tissue-like architecture and structure. (sigmaaldrich.com)
Incubate organoids for 1 day at 37 °C, 5% CO 2 . (sigmaaldrich.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)

It is concluded that the proton-coupled amino acid transporter hPAT1 is the main transporter responsible for absorption of vigabatrin in Caco-2 cells. (ku.dk)
Use of transporter knockdown Caco-2 cells to investigate the in vitro efflux of statin drugs. (degruyter.com)

RNA and immunoblot analysis of both cell lines demonstrate that only the α1 and β1 isoforms are expressed in nonpolarized and polarized cultures. (wustl.edu)
Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)

Spectrofluorimetric measurements showed that cell stimulation with ATP induced transient elevations in intracellular calcium concentration. (conicet.gov.ar)
In addition, western blot analysis revealed that ATP-induced phosphorylation of MAPKs in Caco-2 cells was dependent on calcium influx and intracellular calcium release, Src-family tyrosine kinases, and partially dependent on the cAMP/PKA and PKC pathways. (conicet.gov.ar)

Afin d'étudier l'effet de l'adoucissement de l'eau du robinet (extraction du calcium) sur les colorations à l'hématoxyline et à l'éosine, cinq (5) séries de lames issues de 30 blocs de tissus pathologiques humains différents inclus en paraffine ont été préparées de la même manière, sauf pour le lavage qui a été effectué avec cinq types d'eau différents. (who.int)
La teneur faible en ions calcium et magnésium et élevée en ions sodium de l'eau douce affecte négativement les résultats de la coloration de routine à l'hématoxyline-éosine. (who.int)

Six different orthodontic accessories were evaluated, three of them made from stainless steel (1 bracket, 2 button, 3 mesh pad) and three made from a gold-coated alloy (4 small mesh pad, 5 button, 6 big mesh pad). (bvsalud.org)

Anatomy12

Organisms7

Diseases5

Chemicals and Drugs19

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes25

Information Science3

Health Care3

Geographicals2

Monolayers13

Monolayer9

Uptake18

Assay2

Intestine3

Apical3

Colon adenocarcinoma3

Effects on cell viability2

Viability5

Significantly6

Proliferation9

Transepithelial6

Oxidative stress1

Membrane Permeability1

Adenocarcinoma3

Cellular1

Toxicity3

Transfection2

Tight junction1

Methods2

Assays2

Absorptive2

Differentiation1

Bioavailability1

Drug candidates1

Cycle arrest3

Concentrations3

Characterization3

Prediction1

Physiology2

Abstract1

Experiments2

Gene5

Colorectal cancer cells4

Inflammation2

Model4

Adhesion2

Protein5

Results1

Enterocytes2

Dose-dependent manner1

Line4

Humans1

Mmol1

Organoids3

Embryonic1

Transporter2

Cultures3

Intracellular2

Calcium2

MeSH1