• We demonstrated by immunoelectron microscopy that listeriolysin O (LLO), phospholipases and other putative virulence-related proteins produced by Listeria monocytogenes were primarily cell-wall-associated when the bacterium infected Caco-2 tissue culture cell monolayers. (nih.gov)
  • Under the conditions of this in vitro model, Caco-2 cell monolayers are not useful for evaluating the Zn availability from foods . (rsc.org)
  • To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. (springer.com)
  • Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous polyethylene terephthalate membranes (PETP) in a 12 well format to form monolayers of polarized cells possessing function similar to intestinal enterocytes. (springer.com)
  • Treatment of Caco-2 cell monolayers with ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), which disrupts intercellular junctions, greatly enhanced the rate of cell infection. (asm.org)
  • Both saquinavir and ritonavir showed polarized transport through Caco-2 cell monolayers in the basolateral to apical direction (secretory pathway), exceeding apical to basolateral transport (absorptive pathway) by factors of 50-70 and 15-25, respectively. (ovid.com)
  • Grepafloxacin and levofloxacin transport by Caco-2 cell monolayers was examined to characterize the intestinal behavior of these quinolones. (aspetjournals.org)
  • Caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. (asm.org)
  • The correlation between the in vitro apparent permeability (P¬app) across Caco-2 monolayers and the in vivo fraction absorbed (fa) is well established.Transwell diagram This application of Caco-2 cells was pioneered in the late 1980s by Ismael Hidalgo, working in the laboratory of Ron Borchardt at the University of Kansas, and Tom Raub, who was at the Upjohn Company at the time. (wikipedia.org)
  • First, considering that poor pharmacokinetic properties accounted for ~40% of drug failures in development in the early 1990s and only ~10% by 2009, an interval in which Caco-2 monolayers were widely used throughout the pharmaceutical industry to predict absorption, it is not unreasonable to attribute some of that shift to this simple yet powerful model. (wikipedia.org)
  • These results suggest that a specific transport system mediates rutin movement across the apical membrane in Caco-2 cells and that metabolic enzymes are important for this process. (hindawi.com)
  • The commonly accepted view that enteroinvasive bacteria enter cells of the intestinal epithelial lining through the apical surface can be challenged in the case of shigellosis. (asm.org)
  • In these experiments, the few bacteria that interacted with the apical surface either bound to microvilli of the cell dome without causing detectable alteration or bound at the level of intercellular junctions at which they demonstrated a limited capacity for paracellular invasion, which permitted subsequent entry through the lateral domain of the cells. (asm.org)
  • showed that transepithelial secretion of quinolones involved common mechanisms of accumulative transport at the basolateral membrane followed by facilitated exit across the apical membrane in Caco-2 cells. (aspetjournals.org)
  • Cell viability and proliferation were measured using Promegas CellTiter-Blue(®) Cell Viability assay, DAPI staining and impedance measurements. (biomedsearch.com)
  • During adhesion assay the cells are incubated with listeria for 30 min but in invasion assay the cell growth is arrested at several time points after infection to monitor the growth and survival rate of listeria in cells. (bio-protocol.org)
  • Adhesion assay is straight forward wherein the bacteria are incubated with Caco-2 cells for 30 min and the bacterial counts for mutants and WT strains are compared to observe any alteration in adhesion properties as a result of mutation. (bio-protocol.org)
  • Bacterial counts obtained at different time points during invasion assay give more information about the survival of listeria in the human cells and the comparison of these counts between mutants and WT strains gives information about the changes in the adaptation of listeria after mutation in human cells. (bio-protocol.org)
  • As such, these cells represent a useful and well-accepted tool for metabolism and transport studies (absorption, permeability and/or secretion of drugs/chemicals across the intestinal mucosa). (abcam.com)
  • The intestinal absorption and metabolism characteristics of the potentially beneficial polyphenol rutin were studied by measuring the intracellular accumulation and transport of rutin into Caco-2 cells with the sensitive and reliable analytical method of HPLC-coupled tandem mass spectrometry. (hindawi.com)
  • In this work, we provide evidence that astrovirus RNA replication and virus assembly occur in contact with cell membranes potentially derived from multiple cell organelles and show that membrane-associated cellular proteins involved in lipid metabolism are required for efficient viral replication. (asm.org)
  • At 0.37 μM, DON enhanced Caco-2 cell metabolism, thereby triggering a moderate increase in cell proliferation. (mdpi.com)
  • Here, we have examined the effect of leptin on sugar and amino acids absorption in the human model of intestinal cells Caco-2 and investigated the transporters involved. (biomedsearch.com)
  • Furthermore, the transport of EGC in ERCs 980 and ERCs 4600 across the Caco-2 cells was enhanced by the transferrin receptor 1 (TfR-1)-mediated absorption pathway, demonstrating that chitosan molecules with low molecular weights of 980 and 4600 Da were beneficial to the absorption of EGC based on the ferritin cage. (rsc.org)
  • Caco-2 cell metallothionein (MT) formation was studied to determine if MT could be used as a proxy for zinc (Zn) absorption in a cell culture model. (rsc.org)
  • These data suggest that Caco-2 cells developed under the culturing and transport conditions defined herein can be used to predict in vivo human absorption of compounds regardless of transport mechanism, viz. (springer.com)
  • P-glycoprotein and multidrug-resistant proteins 2 and 3 were involved in the transmembrane transport and intracellular accumulation of rutin by Caco-2 cells. (hindawi.com)
  • Intracellular GSH levels are higher in actively proliferating cells ( 7 , 34 ) and decrease gradually during serum starvation as cells become quiescent ( 34 ). (physiology.org)
  • Listeria monocytogenes being a facultative intracellular bacterium can enter, survive and multiply in both phagocytic and non-phagocytic cells and this property of the bacterium has been extensively studied and understood. (bio-protocol.org)
  • METHODS: Substrate uptake experiments were performed in Caco-2 cells, grown on plates, in the presence and the absence of leptin and the expression of the different transporters in brush border membrane vesicles was analysed by Western blot. (biomedsearch.com)
  • demonstrated that the uptake of sparfloxacin was a passive diffusion mechanism by the brush-border membrane of Caco-2 cells. (aspetjournals.org)
  • GG reduced AFB 1 uptake and protected against both membrane and DNA damage in the Caco-2 model. (asm.org)
  • Caco-2 cells express tight junctions, microvilli, and a number of enzymes and transporters that are characteristic of such enterocytes: peptidases, esterases, P-glycoprotein, uptake transporters for amino acids, bile acids, carboxylic acids, etc. (wikipedia.org)
  • In this study, Caco-2 cells from 10 different laboratories were compared in terms of mRNA expression levels of 72 drug and nutrient transporters, and 17 other target genes, including drug metabolising enzymes, using real-time PCR. (nih.gov)
  • The cellular proteins associated with this membrane population in infected and mock-infected cells were identified by tandem mass spectrometry. (asm.org)
  • A 24-h treatment with DFMO caused a dose-dependent decrease in the colony-forming ability of cells expressing an activated K- ras but had no effect on the viability of the parental Caco-2 cells. (aacrjournals.org)
  • As a result, over the years the characteristics of the cells used in different laboratories around the world have diverged significantly, which makes it difficult to compare results across labs. (wikipedia.org)
  • The redox state of the GSH system becomes oxidized when cells are induced to differentiate by chemical agents. (physiology.org)
  • CONCLUSION: These data show in human intestinal cells that leptin can rapidly control the activity of physiologically relevant transporters for rich-energy molecules, i.e D-glucose (SGLT1) and amino acids (ASCT2, B(0) AT1 and PAT1). (biomedsearch.com)
  • The versatility of Caco-2 cells is demonstrated by the fact that, even to this day, they are serving as the basis for the creation of innovative new models that are contributing to our understanding of drug efflux transporters such as P-glycoprotein (ABCB1) and BCRP (ABCG2). (wikipedia.org)
  • considerable evidence demonstrates that redox signaling mechanisms function in cell regulation and growth control. (physiology.org)
  • GSH depleting agents such as buthionine sulfoximine inhibit cell growth in cultured cells. (physiology.org)
  • We evaluated the effects of the major sulfide and sulfone metabolites of sulindac and DFMO alone, or in combinations, on the growth and survival of Caco-2 colon cancer-derived cells and in clones of these cells transfected with an activated K- ras oncogene. (aacrjournals.org)
  • Finally, comet-tail pseudopod structures shown to be involved in cell-to-cell passage of Listeria in Caco-2 cells did not possess detectable Listeria antigens on their anterior surface or within their structure, suggesting that the phagocytic process is primarily host-cell-dependent once it is initiated by the bacterial cell. (nih.gov)
  • Since positive-sense RNA viruses have frequently been found to replicate in association with membranous structures, in this work we characterized the replication of the human astrovirus serotype 8 strain Yuc8 in Caco-2 cells, using density gradient centrifugation and free-flow zonal electrophoresis (FFZE) to fractionate cellular membranes. (asm.org)
  • Immunogold labelling of Listeria monocytogenes virulence-related factors within Caco-2 cells. (nih.gov)
  • However, clonogenic survival, measured 2 weeks after drug treatment, was the same in both Caco-2 and ras -transfected Caco-2 cells treated with sulindac metabolites. (aacrjournals.org)
  • The results indicated that membranes derived from multiple cell organelles were present in the population. (asm.org)
  • These results strongly suggest that astrovirus RNA replication and particle assembly take place in association with modified membranes potentially derived from multiple cell organelles. (asm.org)