• Immunohistochemistry showed that ZO-1 and occludin were localized to the cell boundaries in control Caco-2 monolayers. (biomedcentral.com)
  • Taken together, our data support the view that CM increases the permeability of Caco-2 monolayers by activating NF-κB and initiating a chain of events that ultimately leads to PARP activation and decreased expression of the tight junction proteins, ZO-1 and occludin. (biomedcentral.com)
  • [7] The correlation between the in vitro apparent permeability (P¬app) across Caco-2 monolayers and the in vivo fraction absorbed (fa) is well established. (wikipedia.org)
  • First, considering that poor pharmacokinetic properties accounted for ~40% of drug failures in development in the early 1990s and only ~10% by 2009, an interval in which Caco-2 monolayers were widely used throughout the pharmaceutical industry to predict absorption, it is not unreasonable to attribute some of that shift to this simple yet powerful model. (wikipedia.org)
  • Caco-2 cells are most commonly used not as individual cells, but as a confluent monolayer on a cell culture insert filter (e.g. (wikipedia.org)
  • When cultured in this format, the cells differentiate to form a polarized epithelial cell monolayer that provides a physical and biochemical barrier to the passage of ions and small molecules. (wikipedia.org)
  • [3] [6] The Caco-2 monolayer is widely used across the pharmaceutical industry as an in vitro model of the human small intestinal mucosa to predict the absorption of orally administered drugs with different kits such as the CacoReady. (wikipedia.org)
  • The considerable impact of the Caco-2 cell monolayer model can be measured in at least two ways. (wikipedia.org)
  • Alkaline phosphatase and peptidase activities were examined as markers for cellular differentiation in the human colon cancer cell lines Caco-2 and HT29 and the minimally transformed NCM460. (researchwithrutgers.com)
  • KLF6 has great importance in the control of various processes as cell growth, cell differentiation, apoptosis and angiogenesis (Gehrau et al. (thefreelibrary.com)
  • The MAP kinase pathway inhibitor U0126 in combination with butyrate promotes differentiation in some colon cancer cell lines. (researchwithrutgers.com)
  • In addition to butyrate, several HDAC inhibitors can induce differentiation in colon cancer cells and the responses may be enhanced by U0126, GW5074 and LY294002. (researchwithrutgers.com)
  • Moyer, Mary P. / Induction of differentiation of colon cancer cells by combined inhibition of kinases and histone deacetylase . (researchwithrutgers.com)
  • The vast majority of these models is based on the human intestinal cell line Caco-2 in combination with other relevant components of the intestinal epithelium, such as mucin-secreting goblet cells and in vitro digestion models, and applying improved compositions of apical and basolateral media to mimic the in vivo situation as closely as possible. (tu-berlin.de)
  • In human Caco-2 intestinal epithelial layers, L-alanine absorption can be energized by a proton gradient across the brush-border membrane. (elsevier.com)
  • By measurement of rapid L-alanine influx across the apical membrane, L-alanine-stimulated inward short-circuit current and intracellular acidification in the same cell batch, we estimate L-alanine/proton stoichiometry to be 1:0.62 +/- 0.25 (SD) (short-circuit current) or 1:0.73 +/- 0.19 (intracellular acidification). (elsevier.com)
  • Journal of Membrane Biology , 140 (2), 143-51. (elsevier.com)
  • Rosiglitazone Role in the Expression of KLF6 Caco-2 Colon Cancer Cells/Rol de Rosiglitazona en la Expresion de KLF6 en Celulas Caco-2 de Cancer de Colon. (thefreelibrary.com)
  • Although derived from a colon ( large intestine ) carcinoma , when cultured under specific conditions the cells become differentiated and polarized such that their phenotype , morphologically and functionally, resembles the enterocytes lining the small intestine . (wikipedia.org)
  • 2005). Furthermore, studies on the expression of SV1 in Prostate Cancer lines found lowered expressions of p21 and increased cell growth (Narla et al. (thefreelibrary.com)
  • Extensive metabolism of the flavonoid chrysin by human Caco-2 and Hep G2 cells. (nih.gov)
  • In an effort better to understand factors that may affect the oral bioavailability of the bioflavonoids from dietary sources, the metabolism of chrysin by cultured intestinal Caco-2 cells and hepatic Hep G2 cells was studied, together modelling human presystemic metabolism. (nih.gov)
  • Lim CP, Phan TT, Lim IJ, Cao X: Stat3 contributes to keloid pathogenesis via promoting collagen production, cell proliferation and migration. (others-signal.com)
  • The versatility of Caco-2 cells is demonstrated by the fact that, even to this day, they are serving as the basis for the creation of innovative new models that are contributing to our understanding of drug efflux transporters such as P-glycoprotein (ABCB1) and BCRP (ABCG2). (wikipedia.org)
  • 2010) there has also been described the antitumor effect of this drug, inhibits the endothelial growth and angiogenesis in models of breast cancer, and induces apoptosis in the cell cycle in colorectal cancer cells (Aires et al. (thefreelibrary.com)
  • The Caco-2 cell line is a continuous line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh. (wikipedia.org)
  • From competition studies, it is likely that L-proline, alpha-aminoisobutyric acid, and beta-alanine, but not L-valine and L-serine, are substrates for proton-linked, substrate transport in the brush border of Caco-2 cells. (elsevier.com)
  • Several HDAC inhibitors caused greater increases of alkaline phosphatase in the cancer cells than in NCM460, in which butyrate was the only HDAC inhibitor that caused a consistent increase. (researchwithrutgers.com)
  • When looking at Caco-2 cell cultures microscopically, it is evident even by visual inspection that the cells are heterogeneous. (wikipedia.org)
  • Transepithelial transport of oral cephalosporins by monolayers of intestinal epithelial cell line Caco-2: specific transport systems in apical and basolateral membranes. (aspetjournals.org)
  • The accumulation and the transepithelial transport of p.o. cephalosporins by monolayers of human intestinal epithelial cell line Caco-2 grown on microporous membrane filters were evaluated. (aspetjournals.org)
  • 1989 ). Basolateral secretion of kappa light chain in the polarised epithelial cell line, Caco-2. (biologists.org)
  • TNFα regulates sugar transporters in the human intestinal epithelial cell line Caco-2. (semanticscholar.org)
  • Biogenetic pathways of plasma membrane proteins in Caco-2, a human intestinal epithelial cell line. (rupress.org)
  • We studied the sorting and surface delivery of three apical and three basolateral proteins in the polarized epithelial cell line Caco-2, using pulse-chase radiolabeling and surface domain-selective biotinylation (Le Bivic, A., F. X. Real, and E. Rodriguez-Boulan. (rupress.org)
  • Binding of Clostridium difficile to Caco-2 epithelial cell line and to extracellular matrix proteins. (microbiologyresearch.org)
  • In this study, the authors used DNA microarray to compare and identify genes induced by HER2 in mammary epithelial cell line with ectopic HER2 overexpression and breast cancer cell lines derived from patients with different level of HER2 expression. (biomedcentral.com)
  • Twenty-five strains of lactobacilli were tested for their ability to adhere to human enterocyte-like Caco-2 cells in culture. (nih.gov)
  • The inlAB operon was cloned and surface-expressed on L. casei resulting in a recombinant strain, LbcInl AB , and subsequently, its ability to inhibit adhesion, invasion and translocation of L. monocytogenes through enterocyte-like Caco-2 cells was examined. (up.ac.za)
  • The current study now demonstrates that mepacrine also inhibits CPE-induced cytotoxicity in human enterocyte-like Caco-2 cells and that mepacrine does not directly inactivate CPE. (cloudfront.net)
  • Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. (definitions.net)
  • Shigella flexneri enters human colonic Caco-2 epithelial cells through the basolateral pole. (asm.org)
  • This study is based on in vitro experiments that showed that the invasion of human colonic Caco-2 cells by Shigella flexneri occurred through the basolateral pole of these cells. (asm.org)
  • 1992 ). A survey of membrane peptidases in two human colonic cell lines, Caco-2 and HT-29. (biologists.org)
  • Whereas Semliki Forest virus infection only occurred when input virions had access to the basolateral domain of MDCK-1 or Caco-2 cells, RRV infected MDCK-1 and Caco-2 monolayers in a symmetric manner. (asm.org)
  • These findings suggest that p.o. cephalosporins accumulate in the Caco-2 cell monolayers via the H+/dipeptide cotransport system localized in the apical membranes and that a specific transport system is involved in the efflux of these antibiotics across the basolateral membranes. (aspetjournals.org)
  • Both saquinavir and ritonavir showed polarized transport through Caco-2 cell monolayers in the basolateral to apical direction (secretory pathway), exceeding apical to basolateral transport (absorptive pathway) by factors of 50-70 and 15-25, respectively. (ovid.com)
  • Decrease of dipeptidyl peptidase IV cell surface expression was not restricted to this protein, since membrane expression of '525' antigen, a basolateral protein and of sucrase-isomaltase, an apically targeted hydrolase, which unlike dipeptidyl peptidase IV mainly follows a direct route to the brush border membrane, also decreases. (biologists.org)
  • Rates of delivery of EGE'-GPI to the two membrane domains in Caco-2 cells, as determined with a biotinylation protocol, revealed apical delivery was approximately 2.5 times that of basolateral. (biologists.org)
  • EGE' delivered to the basolateral cell surface was transcytosed to the apical surface. (biologists.org)
  • However, the mis-sorting of a proportion of EGE'GPI to the basolateral surface of Caco-2 cells provides an explanation for additional sorting signals in the ectodomain of some endogenous GPI-anchored proteins. (biologists.org)
  • 1992 ). The post-synthetic sorting of endogenous membrane proteins examined by simultaneous purification of apical and basolateral membrane fractions from Caco-2 cells. (biologists.org)
  • Immunoprecipitation of alpha-glucosidase from culture medium derived from either the apical or basolateral site of radiolabelled Caco-2 cells, showed that 70-80% of the total amount of precursor form present in the medium is secreted from the apical membrane. (uu.nl)
  • Furthermore, immunocytochemistry showed the presence of a precursor form of alpha-glucosidase on the apical, but not the basolateral, membrane of the Caco-2 cells. (uu.nl)
  • Endocytic pathways in polarized Caco-2 cells: identification of an endosomal compartment accessible from both apical and basolateral surfaces. (rupress.org)
  • Using a transferrin-peroxidase conjugate to delineate the morphological pathway, we have identified an early endocytic compartment in the basolateral cytoplasm of the cells. (rupress.org)
  • Using a second double labeling procedure in which antitransferrin receptor antibody complexed to gold was applied to the basolateral surface for up to 2 h and free peroxidase applied to the apical surface for shorter periods, we demonstrated that this apical marker rapidly (within 5 min) reached endosomes containing antibody-gold. (rupress.org)
  • Our results indicate that, in Caco-2 cells, the endocytic pathways from the apical and basolateral surfaces meet in an endosomal compartment from which transferrin can still be recycled. (rupress.org)
  • Thus, compared with two other well-characterized epithelial models, MDCK cells and the hepatocyte, Caco-2 cells have an intermediate sorting phenotype, with apical proteins using both direct and indirect pathways, and basolateral proteins using only direct pathways, during biogenesis. (rupress.org)
  • Results from cells dosed at 10 μM for 24 hours with AHBA, Roxarsone, or Acetarsone indicated 6%-29% permeation occurring from apical to basolateral side, modeling absorption across intestinal epithelium to the circulatory system. (scirp.org)
  • The aim of this work was to transfect genetic material encoding the peptide, human calcitonin (hCT) into Caco-2 cells (an intestinal epithelial cell model, Hidalgo et aL, 1989) and to examine secretion, in particular from the basolateral surface. (ucl.ac.uk)
  • Here, we describe the protocol for the spontaneous differentiation of Caco-2 cells and the detection of autophagy using Western blot, flow cytometry, and immunofluorescence. (springer.com)
  • The enterocytic differentiation of Caco-2 cells, a human colon adenocarcinoma cell line, is accompanied by the transcriptionally regulated expression of a subset of proteins and their correct sorting towards the cell surface. (biologists.org)
  • Modulation of Cl-/OH- exchange activity in Caco-2 cells by nitric oxide. (biomedsearch.com)
  • Our results demonstrate that NO inhibits Cl-/OH- exchange activity in Caco-2 cells via cGMP-dependent protein kinases G (PKG) and C (PKC) signal-transduction pathways. (biomedsearch.com)
  • We conclude that cAMP induces GSTα and MRP2 expression and activity in Caco-2 cells via the PKA pathway, thus regulating detoxification of specific xenobiotics. (deepdyve.com)
  • Instead, this drug reduces both CPE pore formation and CPE pore activity in Caco-2 cells. (cloudfront.net)
  • Curiel, David T. / Transport across a polarized monolayer of Caco-2 cells by transferrin receptor-mediated adenovirus transcytosis . (elsevier.com)
  • abstract = "Background: human colon cancer cells have been found to express gastrin receptors and a role for gastrin in the growth of colon cancer has been postulated. (elsevier.com)
  • P-glycoprotein and multidrug-resistant proteins 2 and 3 were involved in the transmembrane transport and intracellular accumulation of rutin by Caco-2 cells. (hindawi.com)
  • Intracellular GSH levels are higher in actively proliferating cells ( 7 , 34 ) and decrease gradually during serum starvation as cells become quiescent ( 34 ). (physiology.org)
  • Enterocyte-like differentiated Caco-2 cells have extra- and intracellular esterases able to de-esterify hydroxycinnamate and diferulate esters. (unboundmedicine.com)
  • In addition, intracellular UDP-glucuronosyltransferases and sulfotransferases existing in Caco-2 cells are able to form the sulfate and the glucuronide conjugates of methyl ferulate, methyl sinapate, methyl caffeate, and methyl p-coumarate. (unboundmedicine.com)
  • Confocal laser scanning microscopy and immuno-electron microscopy studies demonstrate that, within 5 minutes of forskolin treatment, the cell surface proteins studied accumulate in intracellular vesicles which were co-labeled with a polyclonal antibody raised against Lamp-1, a lysosomal membrane marker. (biologists.org)
  • Listeria monocytogenes being a facultative intracellular bacterium can enter, survive and multiply in both phagocytic and non-phagocytic cells and this property of the bacterium has been extensively studied and understood. (bio-protocol.org)
  • It was expected that information on the intracellular sorting of hCT by the Caco-2 cell model would give valuable insight into peptide processing by GI epithelial cells and have implications for the oral delivery of the peptide. (ucl.ac.uk)
  • Cytotoxicity of peptide-coated silver nanoparticles on the human intestinal cell line Caco-2. (biomedsearch.com)
  • In order to investigate the effects of silver nanoparticles on human intestinal cells, we used the Caco-2 cell line and peptide-coated silver nanoparticles with defined colloidal, structural and interfacial properties. (biomedsearch.com)
  • It is likely that the strong persistence of the aluminium hydroxide layer surrounding these nanoparticles protects the cells from a direct contact with the potentially phototoxic TiO2 core. (duke.edu)
  • The present study was designed as a comparative proteomic analysis of Caco-2 cells, used as an in vitro model of the small intestine, exposed to 30 nm citrate stabilized-silver nanoparticles (AgNPs) for 24 or 72 h. (europa.eu)
  • Curcumin attenuates acrylamide-induced cytotoxicity and genotoxicity in HepG2 cells by ROS scavenging," Journal of Agricultural and Food Chemistry , vol. 56, no. 24, pp. 12059-12063, 2008. (hindawi.com)
  • Exposure of proliferating Caco-2 cells to silver nanoparticle induced decreasing adherence capacity and cytotoxicity, whereby the formation of reactive oxygen species could be the mode of action. (biomedsearch.com)
  • Additionally, in order to assess a possible effect of the mucus layer in vitro, a cytotoxicity comparison between a monoculture (Caco-2 cells) and a co-culture (Caco-2 and HT29-MTX mucus-secreting cells) model has been performed with selected B. cereus supernatants. (mdpi.com)
  • Furthermore, pre-exposure of Caco-2 cells to LbcInl AB significantly reduced L. monocytogenes-induced cell cytotoxicity and epithelial barrier dysfunction. (up.ac.za)
  • This drug was found to reduce CPE-induced cytotoxicity in Caco-2 cells. (cloudfront.net)
  • The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of-flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. (sigmaaldrich.com)
  • Furthermore, the transport of EGC in ERCs 980 and ERCs 4600 across the Caco-2 cells was enhanced by the transferrin receptor 1 (TfR-1)-mediated absorption pathway, demonstrating that chitosan molecules with low molecular weights of 980 and 4600 Da were beneficial to the absorption of EGC based on the ferritin cage. (rsc.org)
  • Caco-2 cell metallothionein (MT) formation was studied to determine if MT could be used as a proxy for zinc (Zn) absorption in a cell culture model. (rsc.org)
  • To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. (springer.com)
  • These data suggest that Caco-2 cells developed under the culturing and transport conditions defined herein can be used to predict in vivo human absorption of compounds regardless of transport mechanism, viz. (springer.com)
  • The human colon carcinoma cell line, Caco-2, is widely used as a model for oral absorption of xenobiotics. (aspetjournals.org)
  • [3] [6] The Caco-2 monolayer is widely used across the pharmaceutical industry as an in vitro model of the human small intestinal mucosa to predict the absorption of orally administered drugs with different kits such as the CacoReady. (wikipedia.org)
  • First, considering that poor pharmacokinetic properties accounted for ~40% of drug failures in development in the early 1990s and only ~10% by 2009, an interval in which Caco-2 monolayers were widely used throughout the pharmaceutical industry to predict absorption, it is not unreasonable to attribute some of that shift to this simple yet powerful model. (wikipedia.org)
  • The human adenocarcinoma cell line, Caco-2, is a model for intestinal absorption. (scirp.org)
  • With respect to the peak response elicited at 10(-8) M, 1 alpha,25-dihydroxy-16-ene-vitamin D3, 1 alpha,25-dihydroxy-23-yne-vitamin D3 and 1 alpha,25-dihydroxy-16,23Z-diene-vitamin D3 suppressed [3H]thymidine incorporation in confluent Caco-2 cells less than 1 alpha,25(OH)2D3. (aspetjournals.org)
  • Caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. (asm.org)
  • Confluent epithelial layers of human Caco-2 cells mimicking the intestinal barrier. (ovid.com)
  • The 8-days post-confluent Caco-2 cells were treated with ZON or a mixture of ZON and imazalil (IMA). (ugent.be)
  • Caco-2 cells are most commonly used not as individual cells, but as a confluent monolayer on a cell culture insert filter (e.g. (wikipedia.org)
  • Confluent Caco-2 cells grown on collagen-coated Transwell plates were dosed on the apical side. (scirp.org)
  • The O-methylated derivatives, ferulic and isoferulic acid, were the only metabolites detected following incubation of Caco-2 cells with caffeic acid. (unboundmedicine.com)
  • This confirmed the formation of glucuronide conjugates and the hydroxylation of ZON during the incubation with Caco-2 cells. (ugent.be)
  • Exposure of B. pilosicoli cultures to 0.05 mM NE prior to incubation with Caco-2 monolayers resulted in more attachment to the monolayer than occurred with non-exposed cultures. (edu.au)
  • Sulfation of 1-naphthol was detected in Caco-2 cells after 2 h of incubation, and was observed to continue for 24 h, resulting in an accumulation of sulfated 1-naphthol. (elsevier.com)
  • Although derived from a colon ( large intestine ) carcinoma , when cultured under specific conditions the cells become differentiated and polarized such that their phenotype , morphologically and functionally, resembles the enterocytes lining the small intestine . (wikipedia.org)
  • A downregulation of FASN effectively inhibits the activity of "HER2-PI3K/Akt axis" and alters the malignant phenotype in colorectal cancer cells. (biomedcentral.com)
  • Under the conditions of this in vitro model, Caco-2 cell monolayers are not useful for evaluating the Zn availability from foods . (rsc.org)
  • TY - JOUR T1 - Characterization of metabolites of hydroxycinnamates in the in vitro model of human small intestinal epithelium caco-2 cells. (unboundmedicine.com)
  • Alterations in the intestinal assimilation of oxidized PUFAs are ameliorated by a polyphenol-rich grape seed extract in an in vitro model and Caco-2 cells. (semanticscholar.org)
  • Combining the digestion, the lactase treatment and the Caco-2 cell culture model may provide a reliable in vitro model for examining flavonoid glucoside bioavailability from foods. (biomedcentral.com)
  • Cobalt, an inhibitor of the prolyl hydroxylase enzymes governing HIF-1α breakdown in the cell, induces transepithelial leakiness in CaCo-2 BBe cell layers in a time and concentration-dependent manner. (springermedizin.de)
  • Furthermore, CPE action in host cells is complex, involving the toxin binding to receptors, receptor-bound CPE oligomerizing into a prepore on the membrane surface, and β-hairpins in the CPE prepore inserting into the membrane to form a pore that induces cell death. (cloudfront.net)
  • The methods were used for the detection of the compounds in in vitro biological samples, obtained with human intestinal Caco-2 cells culture experiments. (ugent.be)
  • Materials and methods: Caco-2 cells were cultured in the presence of either TO901317, farnesoid X receptor (FXR) antagonist guggulsterone or TO901317 together with guggulsterone at different concentrations for 24 hrs. (lu.se)
  • Methods: cells were grown in Dulbecco's Modified Eagle's medium supplemented with 10% fetal calf serum or in serum- free medium containing pentagastrin and/or proglumide, benzotript or CR 2093. (elsevier.com)
  • Methods and results: In the new protocol, Caco-2 cells were cultured with the classical Caco-2 medium supplemented with puromycin. (archives-ouvertes.fr)
  • Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous polyethylene terephthalate membranes (PETP) in a 12 well format to form monolayers of polarized cells possessing function similar to intestinal enterocytes. (springer.com)
  • In order to understand this virus-cell interaction more completely, we utilized a cell culture-adapted rhesus rotavirus (RRV) to infect human intestinal (Caco-2) and Madin-Darby canine kidney (MDCK-1) polarized epithelial cells grown on a permeable support. (asm.org)
  • Rotavirus infection on filter-grown Caco-2 cells caused a transmembrane leak at 18 h postinfection, before the development of the cytopathic effect (CPE) and extensive virus release. (asm.org)
  • When rotavirus infects the mature villus tip cells of the small intestine, it encounters a highly polarized epithelium. (asm.org)
  • The effect of cAMP on expression and activity of GST and MRP2 was evaluated in Caco-2 cells, a model of intestinal epithelium. (deepdyve.com)
  • These data suggest the potential clinical benefit under conditions where drug delivery is a challenge, such as within the airway epithelium, at the bladder lumen urothelial cell interface, and across the blood-brain barrier for clinical treatment of lung, urogenital, and brain disorders, respectively, by adenoviral transcytosis of transgene delivery. (elsevier.com)
  • We investigated whether differentiated Caco-2 cell filter-cultures would make a more suitable model for studying gene transfer to an epithelium. (monash.edu)
  • Differentiated Caco-2 cells are a more appropriate model for gene-transfer studies to the intestinal epithelium because they demonstrate a resistance to transfection similar to that observed in vivo. (monash.edu)
  • Van Beers EH, Al RH, Rings EH (1995) Lactase and sucrase-isomaltase gene expression during Caco-2 cell differentiation. (springer.com)
  • Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell lines. (biomedsearch.com)
  • Rosiglitazone Role in the Expression of KLF6 Caco-2 Colon Cancer Cells/Rol de Rosiglitazona en la Expresion de KLF6 en Celulas Caco-2 de Cancer de Colon. (thefreelibrary.com)
  • 2005). Furthermore, studies on the expression of SV1 in Prostate Cancer lines found lowered expressions of p21 and increased cell growth (Narla et al. (thefreelibrary.com)
  • To study Rab21 protein expression in Caco-2 cells, polyclonal antibodies were raised against GST-Rab21 fusion protein and characterised. (nih.gov)
  • Furthermore, immunohistochemical staining on human jejunal tissue showed a predominant expression of Rab21 in the epithelial cell layer with high expression levels in the apical region, whereas stem cells in the crypts were negative. (nih.gov)
  • In the present work we have explored the possibility that post-translational events may interfere with this process by investigating the short term effects of a potent adenylyl cyclase activator, forskolin, on cell surface expression of dipeptidyl peptidase IV. (biologists.org)
  • Domain specific biochemical experiments demonstrate that cell surface expression of neosynthesized dipeptidyl peptidase IV rapidly decreases after a 1 hour forskolin treatment. (biologists.org)
  • We report that treatment of Caco-2 cells with 1α,25-dihydroxyvitamin D 3 , beginning at confluence, results in a dose- and duration-dependent increase in CYP3A4 mRNA and protein, with little apparent effect on the expression of CYP3A5 or CYP3A7. (aspetjournals.org)
  • 1992 ). Expression and polarized apical secretion in Madin-Darby canine kidney cells of a recombinant soluble form of neutral endopeptidase lacking the cytosolic and transmembrane domains. (biologists.org)
  • Cells incubated with the cAMP permeable analog dibutyryl cyclic AMP (db-cAMP: 1,10,100μM) for 48h exhibited a dose-response increase in GST class α and MRP2 protein expression. (deepdyve.com)
  • Consistent with increased expression of GSTα and MRP2, db-cAMP enhanced their activities, as well as cytoprotection against the common substrate 1-chloro-2,4-dinitrobenzene. (deepdyve.com)
  • SREBP-2 gene or mature protein expression was not altered. (biochemj.org)
  • Liver X receptor (LXR) activation by T0901317 increased gene expression of SREBP-1c, SREBP-1a, FAS and acetyl-CoA carboxylase without altering SREBP-2. (biochemj.org)
  • Although only 18:2, 20:4, 20:5 and 22:6 suppressed SREBP-1, acetyl-CoA carboxylase and FAS expression, all fatty acids decreased the rate of fatty acid synthesis. (biochemj.org)
  • In CaCo-2 cells, polyunsaturated fatty acids decrease gene and protein expression of SREBP-1 and FAS mRNA, probably through interference with LXR activity. (biochemj.org)
  • Mechanism of TNF-{alpha} modulation of Caco-2 intestinal epithelial tight junction barrier: role of myosin light-chain kinase protein expression. (semanticscholar.org)
  • The TNFα effect was associated with a redistribution of the sealing TJ protein claudin-1, an increase in the expression of claudin-2 and a change in TJ ultrastructure. (springermedizin.de)
  • Cell surface expression of InlAB on the LbcInl AB was confirmed by Western blotting and immunofluorescence staining. (up.ac.za)
  • Because it was previously demonstrated that nitrosamines and nitrosamides, representing two classes of NOC, induce distinct gene expression effects in colon cells that are particularly related to oxidative stress, we hypothesized that different radical mechanisms are involved. (oup.com)
  • These findings suggest that following NOC exposure in Caco-2 cells, ROS formation plays an important role in deregulation of gene expression patterns that may be relevant for the process of chemical carcinogenesis in the human colon, in addition to the role of DNA alkylation. (oup.com)
  • Moreover gene expression analysis using pangenomic oligomicroarrays (4x 44000 genes) did not show any change versus unexposed cells after exposure to 10 μg/ mL, which is much higher than potential environmental concentrations. (duke.edu)
  • In the present study we further investigated regulation of apoM expression in Caco-2 cells stimulated by a liver X receptor (LXR) agonist, TO901317. (lu.se)
  • Conclusion: The present study demonstrated that LXR agonist TO901317 induced apoM expression in Caco-2 cells might be mediated via the LXR/FXR pathway. (lu.se)
  • We had previously found that carnosine augmented the expression of BDNF in human colorectal cancer cells, thus we became interested in cAMP-responsive element binding protein (CREB), which is a dominant regulator of BDNF transcription. (elsevier.com)
  • Each array represents a measurement of human mRNA from cultured intestinal epithelial cells (red channel), relative to the control reference pool of mRNA (green channel), at different time points during the course of an infection by Listeria monocytogenes, or the mutants actA (defective in actin-based motility) and prfA (defective in virulence factor gene expression). (omicsdi.org)
  • Caco-2 cells with a high expression of both HER2 and FASN were selected for functional characterization. (biomedcentral.com)
  • Caco-2 cells were transfected with either the FASN specific RNAi plasmid or the negative control RNAi plasmid, followed by the RT-qPCR and western blot to examine the expression of FASN, HER2, PI3K and Akt. (biomedcentral.com)
  • However, in cancer cells and pre-neoplastic lesions, FASN expression has been frequently found to be upregulated. (biomedcentral.com)
  • PI3K activity was thought to be the mediator of the HER2 control on FASN expression because LY294002, a known PI3K inhibitor, abrogated HER2 induced FASN protein production in the HER2 overexpressing normal mammary epithelial and breast cancer cells. (biomedcentral.com)
  • It also influences the pattern of gene expression of the cells in which it is in contact. (corning.com)
  • Finally, siRNA-mediated functional knock down of HNF4α repressed TRPC1 gene expression in cell culture experiments. (diabetesjournals.org)
  • Furthermore, aspirin treatment is associated with an increase in bcl-2 expression, which persists in the presence of the anticancer drugs. (elsevier.com)
  • All mammalian expression constructs were also transfected into COS cells. (ucl.ac.uk)
  • Results are discussed in relation to calcitonin gene expression and suggestions are made as to how one might proceed with the project by examination of gene transcription and subsequent expression of hCT or its precursors in Caco-2 cells. (ucl.ac.uk)
  • We found that coffee reduced SULT1E1 gene expression in human colon carcinoma Caco-2 cells. (elsevier.com)
  • Treatment with 2.5% (v/v) coffee for 24 h resulted in a 60% reduction of the expression of the SULT1E1 gene in Caco-2 cells. (elsevier.com)
  • Dose-Dependent Targeted Suppression of P-glycoprotein Expression and Function in Caco-2 Cells. (althotas.com)
  • The dose-dependent nature of the Pgp silencing in this study offers significant improvements over other approaches to creating a Caco-2 model with suppressed ABCB1 expression. (althotas.com)
  • Lipid hydroperoxide induced oxidative stress damage and antioxidant enzyme response in Caco-2 human colon cells. (semanticscholar.org)
  • Growth inhibitory effects on human colon adenocarcinoma-derived Caco-2 cells and calcemic potential of 1 alpha,25-dihydroxyvitamin D3 analogs: structure-function relationships. (aspetjournals.org)
  • C-1, 16, 23, 26 and 27, were tested for their growth inhibitory and prodifferentiating potency in human colon adenocarcinoma-derived Caco-2 cells. (aspetjournals.org)
  • Using electron spin resonance spectroscopy, we investigated the radical-generating properties of genotoxic NOC concentrations in human colon adenocarcinoma cells (Caco-2). (oup.com)
  • A 1.5 μM concentration of DON maintained cell adherence of non-proliferating Caco-2 cells, whilst arresting the growth of actively proliferating cells compared with control Caco-2 cells in vitro . (mdpi.com)
  • Previously, we showed that infecting human intestinal epithelial cells (Caco-2) with rotavirus (RV) increases the release of extracellular vesicles (EVs) with an immunomodulatory function that, upon concentration at 100,000×g, present buoyant densities on a sucrose gradient of between 1.10 to 1.18 g/ml (characteristic of exosomes) and higher than 1.24 g/ml (proposed for apoptotic bodies). (sigmaaldrich.com)
  • Inflammatory parameters in Caco-2 cells: effect of stimuli nature, concentration, combination and cell differentiation, Toxicol In Vitro. (oroxcell.com)
  • The initial cell seeding density was increased two times compared to the traditional procedure and the presence of a low concentration of puromycin in the culture medium reduced the Caco-2 permeability of mannitol. (archives-ouvertes.fr)
  • Radioimmunoassay (RIA) of cell medium and cell lysates subjected to concentration on a Sep-Pak Cig cartridge confirmed secretion of rCT by 6-23 cells. (ucl.ac.uk)
  • These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway. (sigmaaldrich.com)
  • In Caco-2 cells, myrrh induced an increase in transepithelial resistance (TER) which was associated with downregulation of the channel-forming tight junction (TJ) protein claudin-2 via inhibition of the PI3 kinase signalling pathway. (springermedizin.de)
  • Heat shock stress ameliorates cytokine mixture-induced permeability by downregulating the nitric oxide and signal transducer and activator of transcription pathways in Caco-2 cells. (semanticscholar.org)
  • Nuclear hormone receptors regulate diverse metabolic pathways and the orphan nuclear receptor LRH-1 (also known as NR5A2) regulates bile acid biosynthesis 1 , 2 . (nature.com)
  • Our findings suggest that either heat-killed probiotics or probiotic cell wall extracts are able to attenuate inflammation through pathways similar to that of live bacteria. (beds.ac.uk)
  • CONCLUSIONS: TiO2 STNPs, degraded or not, are not harmful to Caco-2 cells and are unlikely to penetrate the body via oral route. (duke.edu)
  • Human intestinal epithelial cells have been used to study how listeria crosses the intestinal barrier and cause infection. (bio-protocol.org)
  • 23 ). We have previously shown that Afa/Dr DAEC strains infect polarized human intestinal Caco-2 cells expressing a well-characterized brush border endowed with CD55 and forming a monolayer mimicking an epithelial barrier ( 32 ). (asm.org)
  • Transepithelial electrical resistance and transepithelial diffusion of 14 C-D-mannitol and other radiolabeled probes are used as indicators of transepithelial barrier function of CaCo-2 BBe human gastrointestinal epithelial cell layers cultured on permeable supports. (springermedizin.de)
  • A genetically modified CaCo-2 variant with reduced levels of HIF-1β, showed reduced transepithelial leak in response to cobalt exposure, further indicating that elevation of HIF-1α levels induced by agents of "chemical hypoxia" is responsible for the compromised barrier function of the CaCo-2 BBe cell layers. (springermedizin.de)
  • In HT-29/B6 cells, myrrh had no effect on barrier properties under basic conditions, but protected against barrier damage induced by TNFα, as indicated by a decrease in TER and an increase in fluorescein permeability. (springermedizin.de)
  • This study shows for the first time that myrrh exerts barrier-stabilising and TNFα-antagonising effects in human intestinal epithelial cell models via inhibition of PI3K and STAT6 signalling. (springermedizin.de)
  • Ikenouchi J et al (2005) Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells. (springermedizin.de)
  • Here, we describe the establishment of a high-throughput screening (HTS) platform based on AlphaLISA and electrical cell-substrate impedance sensing (ECIS) technology for the identification of anti-inflammatory and barrier-protective compounds in human enterocytes after pepsin-trypsin-digested gliadin (PT-gliadin) treatment. (gallusimmunotech.com)
  • When cultured in this format, the cells differentiate to form a polarized epithelial cell monolayer that provides a physical and biochemical barrier to the passage of ions and small molecules. (wikipedia.org)
  • Department of Cell Biology and Anatomy, Cornell University Medical College, New York 10021. (rupress.org)
  • Less-differentiated cell layers were significantly more affected than well-differentiated cell layers regarding induced transepithelial leak. (springermedizin.de)
  • Over the years, the characteristics of the cells used in different laboratories around the world have diverged significantly, which makes it difficult to compare results across labs. (wikipedia.org)
  • The addition of the antioxidant, superoxide dismutase, significantly reversed the bactericidal activity of both Caco-2 cells and neutrophils for P. aeruginosa and S. aureus, while catalase had no effect. (ovid.com)
  • Adherence to Caco-2 cells was significantly reduced when C. difficile spores were treated with trypsin. (microbiologyresearch.org)
  • Sonication of C. difficile spores altered the ultrastructure of the outermost exosporium-like structure, releasing two protein species of ~40 kDa and significantly reduced spore hydrophobicity and adherence to Caco-2 cells. (microbiologyresearch.org)
  • Exposure to 0.05 mM NE, but not to higher concentrations, also resulted in significantly more spirochaete cells entering capillary tubes containing 4% porcine gastric mucin than occurred with untreated cultures. (edu.au)
  • In vitro peripheral blood mononuclear cell stimulation by F. prausnitzii led to significantly lower IL-12 and IFN-γ production levels and higher secretion of IL-10. (pnas.org)
  • Additional file 1: Representative western immunoblot probed for Claudin-5, showing effect of cobalt exposure of CACO-2 cell layers. (springermedizin.de)
  • Additional file 3: Representative western immunoblot probed for HIF-1a, showing effect of cobalt exposure of CACO-2 cell layers. (springermedizin.de)
  • Additional file 5: Representative western immunoblot probed for Occludin, showing effect of cobalt exposure of CACO-2 cell layers. (springermedizin.de)
  • N-methyl-N-nitrosourea exposure resulted in a small ROS signal, and formation of nitrogen-centered radicals (NCRs), also stimulated by Caco-2 cells. (oup.com)
  • Stem cells are required for the regeneration process, and can be eradicated by exposure to radiation. (omicsdi.org)
  • Flow cytometry cell cycle analysis confirmed accumulation in S phase from 21% (control) to 36% (24 hour exposure to 10 μM AHBA). (scirp.org)
  • Immunogold labelling of Listeria monocytogenes virulence-related factors within Caco-2 cells. (nih.gov)
  • Finally, comet-tail pseudopod structures shown to be involved in cell-to-cell passage of Listeria in Caco-2 cells did not possess detectable Listeria antigens on their anterior surface or within their structure, suggesting that the phagocytic process is primarily host-cell-dependent once it is initiated by the bacterial cell. (nih.gov)
  • Several cancerous cell lines like cervical, liver, dendritic, intestinal and macrophages have been used to study in vitro propagation and survival of listeria in human cells. (bio-protocol.org)
  • 2. At concentrations that may be achieved in the diet, chrysin was extensively metabolized to two conjugated metabolites, M1 and M2, with no CYP-mediated oxidation. (nih.gov)
  • We evaluated the effects of the major sulfide and sulfone metabolites of sulindac and DFMO alone, or in combinations, on the growth and survival of Caco-2 colon cancer-derived cells and in clones of these cells transfected with an activated K- ras oncogene. (aacrjournals.org)
  • However, clonogenic survival, measured 2 weeks after drug treatment, was the same in both Caco-2 and ras -transfected Caco-2 cells treated with sulindac metabolites. (aacrjournals.org)
  • The ECM is composed of a number of different macromolecules that influence such cell behaviors as adherence, spreading, differentiation, and migration. (corning.com)
  • 2. The method of claim 1, wherein said Gram (+) bacteria and glutamine composition protects tissues along the gastro-intestinal tract by blocking bacteria or other agent adherence to mucosal layers and by blocking bacteria or other agent translocation across said mucosal layers. (freepatentsonline.com)
  • Caco-2 cells possess many of the properties of the small intestine. (abcam.com)
  • This enterotoxin is produced when C. perfringens sporulates in the intestine, and it is then released upon lysis of the mother cell ( 1 ). (cloudfront.net)
  • The considerable impact of the Caco-2 cell monolayer model can be measured in at least two ways. (wikipedia.org)
  • The accelerated Caco-2 model has been compared to the traditional model (requiring 21-25 days of culture) in terms of tightness of the junctions, ability to rank chemical compounds for apparent permeability, active efflux and to discriminate P-gp substrates. (archives-ouvertes.fr)
  • Drug permeability studies using the in vitro Caco-2 cell model commonly rely on small molecule modulators to estimate the contribution of Pgp to drug efflux. (althotas.com)
  • In this study we demonstrate the dose-dependent, targeted degradation of Pgp in Caco-2 cells as a new model for assessing drug efflux from enterocytes. (althotas.com)
  • To investigate biological activity of usnic acid derivatives, enantiopure derivatives were synthesised by reacting usnic acid with ethylenediamine, which yielded one dimer product ((+)-1), and two tetra cyclic compounds ((+)-2 and (-)-2). (ruc.dk)
  • The same rank order was obtained for the ability of the vitamin D compounds to induce activity of the differentiation marker enzyme, alkaline phosphatase, in quiescent Caco-2 cells. (aspetjournals.org)
  • We found proliferative effects on Caco-2 cells at micromolar levels of these compounds, as monitored by [ 3 H]-thymidine incorporation into DNA. (scirp.org)
  • The intestinal transport of compounds can be measured in vitro with Caco-2 cell monolayers. (uu.nl)