• The Cyagen Knockout Catalog Models repository provides a searchable listing of over 16k mouse strains in more than 20 research fields, including oncology, cardiology, and neurology. (cyagen.com)
  • You can quickly query the required genetically engineered global knockout (KO) mouse, conditional knockout (cKO/floxed) mouse and microRNA KO mouse strains by simply entering the gene name. (cyagen.com)
  • Cyagen 녹아웃 카탈로그 모델 저장소는 종양학, 심장학 및 신경학을 포함한 20개 이상의 연구 분야에서 16,000개 이상의 mouse strains 의 검색 가능한 목록을 제공합니다. (cyagen.com)
  • 유전자 이름만 입력하면 필요한 유전자 공학 global knockout (KO) mouse, conditional knockout (cKO/floxed) mouse 및 microRNA KO mouse strains을 빠르게 쿼리할 수 있습니다. (cyagen.com)
  • 2018). Here, we show that cilia loss is seen as early as 10 weeks of age in these mice and also in two other mouse strains carrying the most common human G2019S LRRK2 mutation. (elifesciences.org)
  • This study was undertaken to compare the sensitivities of mice strains during tumor induction by transcription activator-like effector nucleases (TALEN)-mediated Trp53 mutant gene. (biomedcentral.com)
  • Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. (gentaur.com)
  • The non-agouti mutation in C57BL/6 strains is due to an 11.8 Kbp retrotransposon located in the first intron of the agouti gene that abolishes the expression of the Agouti gene. (umassmed.edu)
  • Virus-specific antibody responses correlated with protection from encephalitis in all mouse strains, suggesting that Tfh-B cell interactions modulate clinical outcome in this model. (cdc.gov)
  • Defects observed in Hat1 +/- mice are consistent with an early-onset aging phenotype. (nih.gov)
  • METHODS: As renal phenotype can be influenced by genetic background, we generated congenic C57BL/6 and FVB/N Ctns(-/-) mice and assayed renal lesions and function by histological and biochemical studies. (cnrs.fr)
  • In addition to highlighting the influence of genetic background on phenotype, the C57BL/6 Ctns(-/-) mice represent a useful model for further understanding cystinosin function in the kidney and, specifically, in the proximal tubules. (cnrs.fr)
  • Loss of the PPM1H phosphatase that is specific for LRRK2-phosphorylated Rab GTPases yields the same cilia loss phenotype seen in mice expressing pathogenic LRRK2 kinase, strongly supporting a connection between Rab GTPase phosphorylation and cilia loss. (elifesciences.org)
  • Large-scale gene trapping in C57BL/6N mouse embryonic stem cells. (nih.gov)
  • To generate suitable C57BL/6 embryonic stem cells for gene targeting experiments, the Sanger team established a male cell line (JM8) from the N-substrain of C57BL/6 mice. (umassmed.edu)
  • Experience has shown that injection of black C57BL/6N embryonic stem cells into albino C57BL/6J Tyrc-Brd blastocysts is a particularly favorable combination for germline transmission. (umassmed.edu)
  • Knock-out (KO) mice lacking FMRP were compared with congenic C57BL/6J wild-type (WT) controls. (jneurosci.org)
  • The p16-3MR mouse model was created and backcrossed to C57BL/6J ( 000664 ) using a "speed congenic" approach. (jax.org)
  • If you have a mutation that you would like to transfer to a different genetic background, JAX offers both a comprehensive Speed Congenic Service as well as a Genome Scanning Service to assist you with backcrossing your mice at your facility. (jax.org)
  • A minimum of four (4) mutant mice, heterozygous for the selected mutation on a mixed 129S5;B6 background. (taconic.com)
  • Our data indicate, for the first time, a requirement for MAPK signaling in the developing XY gonad in order to facilitate normal expression of SRY and the downstream testis-determining genes and also suggest that reduced dosage of MAP3K4 may be the cause of a previously described autosomal sex-reversing mutation in the mouse. (plos.org)
  • The technology of sperm cryopreservation is widely used for storage of genetically engineered mice produced by global large-scale knockout mice or mouse mutagenesis projects in mouse banks. (go.jp)
  • In addition, these ES cells are the foundation for two large-scale knockout mouse programs designed to provide targeted BL/6 ES cells to the scientific community (EUCOMM and KOMP). (umassmed.edu)
  • Some lines are stored as cryopreserved embryos and when revitalized will result in a 129/S5 x C57BL/6J- Tyr c-Brd background. (taconic.com)
  • CRISPR/Cas9-mediated NHEJ in mouse embryos will be used to generate founder animals harboring insertion/deletion (indel) or interval deletion knockout alleles. (bcm.edu)
  • 80 C57BL/6J, C57BL/6N, or FVB/NJ embryos collected from superovulated females will be electroporated and transferred to pseudopregnant females. (bcm.edu)
  • If the guide RNAs were (i) tested in mouse embryos by the core and the core approved their use based on the genotyping results and (ii) the core conducted the founder genotyping, the core can review results, redesign the approach and reagents if needed, and the electroporation can be repeated. (bcm.edu)
  • If the guide RNAs were not tested in mouse embryos or the investigator conducts their own genotyping, there will be no reduced costs for additional work. (bcm.edu)
  • 1991. Effects of polychlorinated biphenyls with Ah receptor affinity on lymphoid development in the thymus and the bursa of Fabricius on chick embryos in ovo and in mouse thymus anlagen in vitro . (cdc.gov)
  • On the C57BL/6J background, embryos homozygous for byg exhibit consistent XY gonadal sex reversal. (plos.org)
  • Established in response to a need for in-house mouse services, the LUTCF provides expertise in cryopreservation of embryos by IVF or natural matings, sperm cryopreservation, rederivation services, strain expansion by IVF, ES morula/blastocyst injections, pronuclear DNA microinjections, and injection of CRISPR edited DNA. (lu.se)
  • This is currently the most popular method for the generation of targeted knock-out and knock-in models by ES-cell injections into 8-cell morulae and/or blastocysts of mouse embryos. (lu.se)
  • Due to compensatory genetic mechanisms, knockout status cannot be guaranteed and will have to be confirmed by the customer. (taconic.com)
  • Pharmacological inhibition, genetic silencing or knockout of Plk1 attenuated obstructive nephropathy due to suppressed fibroblast activation mediated by reduced autophagic flux. (nature.com)
  • In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
  • Each of the PCR-positive blood samples was injected into 2 adult type I interferon receptor-knockout mice on a C57BL/6 genetic background. (cdc.gov)
  • Genetic manipulation of the mouse genome has been customarily performed using ES cells derived from the agouti 129-inbred strain of mouse. (umassmed.edu)
  • However, the resulting targeted G1 mice are of a mixed 129 x C57BL/6 genetic background. (umassmed.edu)
  • These ES cells allow for the generation of gene‐targeted mice on a C57BL/6 genetic background without the need for multiple backcrossing of mice. (umassmed.edu)
  • However, a disadvantage of this combination is that a slightly mixed C57BL/6 genetic background (C57BL/6N x C57BL/6J) is produced when breeding the resulting chimeras with C57BL/6J Tyrc-Brd albino mice to test for germline transmission in the G1 generation. (umassmed.edu)
  • Vitamin E status and metabolism in adult and aged aryl hydrocarbon receptor null mice. (oregonstate.edu)
  • CDK2-deficient mice displayed enhanced resistance to cisplatin toxicity in cochlear explants and to cisplatin- and noise-induced hearing loss in vivo. (nih.gov)
  • To investigate a possible synaptic function of tau, we studied synaptic plasticity in the hippocampus and found a selective deficit in long-term depression (LTD) in tau knockout mice in vivo and in vitro, an effect that was replicated by RNAi knockdown of tau in vitro. (bath.ac.uk)
  • TNF-α-mediated bystander assays used a murine coculture system of primary colonic epithelial cells and E. faecalis -infected macrophages ( in vitro ), with an interleukin 10 (IL-10)-deficient mouse model of colon cancer that involves long-term colonization with E. faecalis ( in vivo ). (aacrjournals.org)
  • In addition, LRG47-deficient mice are highly susceptible to LPS, but not TLR2 ligand-induced shock, an outcome that correlates with enhanced proinflammatory cytokine production in vitro and in vivo. (duke.edu)
  • We found that GGTase-I knockout attenuated diabetes-accelerated atherosclerosis in vivo and suppressed high-glucose-induced VSMC proliferation in vitro. (hindawi.com)
  • A 3-component transgenic mouse model to track and manipulate senescent cells in vivo . (jax.org)
  • Using these p16-3MR mice and in vivo bioluminescent imaging, the researchers observed a robust, but transient, increase in senescent cells at the site of cutaneous injury. (jax.org)
  • Lines are stored primarily as cryopreserved sperm on a mixed 129S5 x C57BL/6J- Tyr c-Brd background. (taconic.com)
  • Lines are recovered using cryopreserved sperm and C57BL/6NTac oocyte donors. (taconic.com)
  • Mouse sperm cryopreservation has greatly developed and spread throughout the world since it was the first successfully performed in 1990. (go.jp)
  • Sperm cryopreservation provides the research community with permanent access to genetically engineered mice and as such is an essential part of research activities. (go.jp)
  • Many researchers have actively been investigating the techniques surrounding mouse sperm cryopreservation and embryo production using cryopreserved sperm. (go.jp)
  • In particular, the topic of improving the low fertility rate of cryopreserved sperm in the C57BL/6 strain of mouse, the most common inbred strain used as a base for genetically engineered mice, has been garnering much attention. (go.jp)
  • This review focuses on recent work regarding various attractive technologies in the fields of sperm cryopreservation and efficient embryo production using cryopreserved C57BL/6 mouse sperm. (go.jp)
  • Thank you for your interest in C57BL/6J-Nfya Mouse Model [Frozen Sperm] . (creative-biolabs.com)
  • The antitumor efficacy was studied in mouse mammary carcinoma models (Her2/neu transgenic and 4T1-luc mammary cancers) treated with systemic recombinant protein with/without the depletion of myeloid-derived suppressor cells or intra-tumoral gene electrotransfer (GET). (frontiersin.org)
  • 1999. Adverse reproductive outcomes in the transgenic Ah receptor-deficient mouse. (cdc.gov)
  • In their study, the Campisi team developed a transgenic mouse model to label and eliminate cells undergoing cellular senescence. (jax.org)
  • Scar tissue (stained for collagen, in orange) is evident in healed wounds from transgenic p16-3MR mice lacking senescent cells (bottom panel, GCV-treated) compared to control mice (top panel, PBS-treated). (jax.org)
  • The UMass Chan Transgenic Animal Modeling Core (TAMC) also has JM8-strain ES cells for targeting, and routinely injects either JM8.F6-strain ES cells or JM8.A3-strain ES cells into albino C57BL/6J Tyrc-Brd blastocysts. (umassmed.edu)
  • and the convenience of performing mouse reproduction via in vitro fertilization. (go.jp)
  • We generated mice that possess a heterozygous deletion of the PTG gene. (jci.org)
  • Although young PTG heterozygous mice initially demonstrate normal glucose tolerance, progressive glucose intolerance, hyperinsulinemia, and insulin resistance develop with aging. (jci.org)
  • Insulin resistance in older PTG heterozygous mice correlates with a significant increase in muscle triglyceride content, with a corresponding attenuation of insulin receptor signaling. (jci.org)
  • Since these JM8A3 cells are heterozygous for the corrected agouti allele [A tm1brd ], crossing the resulting brown-on-white chimeras with C57BL/6N test mice yield embryonic stem cell-derived offspring with either agouti or black coats. (umassmed.edu)
  • Similar tumors were also observed in KO mice having the exon 2 or exon 2-6 deletion in C57BL/6 mice. (biomedcentral.com)
  • The rise in right ventricular systolic pressure (RVSP) normally observed following chronic hypoxic exposure was absent in mice with pulmonary endothelial HIF-2α deletion. (cam.ac.uk)
  • The RVSP of mice lacking HIF-2α in pulmonary endothelium after exposure to hypoxia was not significantly different from normoxic WT mice and much lower than the RVSP values seen in WT littermate controls and mice with pulmonary endothelial deletion of HIF-1α exposed to hypoxia. (cam.ac.uk)
  • Endothelial HIF-2α deletion also protected mice from hypoxia remodeling. (cam.ac.uk)
  • This phenomenon has been modeled in C57BL/6 mice depleted of CD4 T cells prior to infection with DelNSs RVFV (RVFV containing a deletion of NSs), resulting in late-onset encephalitis accompanied by high levels of viral RNA in the brain in 30% of animals. (cdc.gov)
  • Our results indicate that the spectrum and incidence of tumors induced by the TALEN-mediated Trp53 mutant gene is greater in FVB/N-Trp53 em2Hwl /Korl KO mice than C57BL/6-Trp53 em1Hwl /Korl KO mice over 16 weeks. (biomedcentral.com)
  • Numerous Trp53 KO mice containing large deletions of the Trp53 gene have been produced to investigate the role of Trp53 during the developmental process or tumor formation in mammals. (biomedcentral.com)
  • This study was therefore undertaken to compared the tumorigenic phenotypes of FVB/N-Trp53 em2Hwl /Korl and C57BL/6-Trp53 em1Hwl /Korl KO mice encompassing the TALEN-mediated Trp53 mutant gene (Fig. 1 ). (biomedcentral.com)
  • A conditional knockout resource for the genome-wide study of mouse gene function. (nih.gov)
  • Using CRISPR/Cas9 technology, sgRNA and ssDNA are designed, and Nfya gene conditional knockout mice are obtained through high-throughput electrotransformation of fertilized eggs. (creative-biolabs.com)
  • The Sanger group performed gene targeting in the JM8 ES cells to delete the retrotransposon from the agouti locus and restore agouti gene function, permitting the visualization of ES cell-derived mice on an inbred C57BL/6 background by agouti coat color. (umassmed.edu)
  • Interleukin-6 (IL-6) seems to regulate both plasmacytoma development in BALB/c mice and oil granuloma formation in C57BL/6 mice during pristane-induced inflammation [ 10 , 12 ]. (hindawi.com)
  • Previous studies from our group and other labs have demonstrated that oil granulomas represent the major pathology in response to pristane injections in C57BL/6 mice [ 2 , 3 ]. (hindawi.com)
  • A molecular explanation for this may be that several of the engulfment genes expressed by macrophages, including the ABC1 transporter (believed to be part of the phagocytic machinery conserved from Caenorhabditis elegans to mouse), are not upregulated by these 'stand-in' phagocytes. (bris.ac.uk)
  • Mice deficient for TNF α develop defective oil granulomas with reduced recruitment of macrophages and neutrophils [ 10 ]. (hindawi.com)
  • The migration of inflammatory leukocytes, including dendritic cells, neutrophils, and macrophages, to the peritoneal mesentery has been shown to be promoted in LAT −/− (lack mature T cells) mice but inhibited in μ MT (lack mature B cells) mice during the pristane-induced immune response [ 10 ]. (hindawi.com)
  • Extending these observations, colon biopsies from E. faecalis -colonized IL-10 −/− mice exhibited crypt hyperplasia and increased staining for macrophages, TNF-α, netrin-1, NF-κB, Tnfrsf1b, and the proliferation marker proliferating cell nuclear antigen while also displaying a reduction in epithelial cell apoptosis. (aacrjournals.org)
  • reported that GGTase-I deficiency in mouse macrophages slowed down the progression of atherosclerosis [ 9 ]. (hindawi.com)
  • To understand the broader biological role of Hat1, we have generated a conditional mouse knockout model of this enzyme. (nih.gov)
  • ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. (gentaur.com)
  • ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. (gentaur.com)
  • ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. (gentaur.com)
  • We find that LTP of monosynaptic responses evoked by selective stimulation of excitatory associational fibers is substantially reduced in slices of anterior piriform cortex (APC) from Fmr1 KO mice, compared with wild-type (WT) controls. (jneurosci.org)
  • We report here that BTA-EG 4 decreases Aβ levels, alters cell surface expression of amyloid precursor protein (APP), and improves memory in wild-type mice. (jneurosci.org)
  • While AND-34 is widely expressed in wild type mice, histologic analysis of multiple organs in AND-34 −/− mice is unremarkable and analyses of lymphocyte development show no overt changes. (molvis.org)
  • We therefore used ultrasound biomicroscopy to assess left ventricular (LV) cardiac structure and function during development in Fgl2 KO and wild-type (WT) mice. (mcmaster.ca)
  • Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. (cdc.gov)
  • B6.129X1-MPO (MPO k/o) vs wild-type C57Bl/6 mice (w/t) and induction of mesotheliomas) [8,9]. (cdc.gov)
  • Male C57BL/6 J wild type (WT) were fed with standard (SD) or high-fat diet (HFD) for 12-weeks. (bvsalud.org)
  • In this study, mouse conditional knockout of VSMC GGTase-I (Pggt1b Δ / Δ mice) was generated using the CRISPR/Cas9 system. (hindawi.com)
  • EUCOMM--the European conditional mouse mutagenesis program. (nih.gov)
  • The knockout models are designed to be null alleles. (taconic.com)
  • Most of these mice showed similar phenotypes as an effective result of the null alleles. (biomedcentral.com)
  • 2012) Impaired Clearance and Enhanced Pulmonary Inflammatory/Fibrotic Response to Carbon Nanotubes in Myeloperoxidase-Deficient Mice. (cdc.gov)
  • CNT in biochemical models and in cells yielding the products engineered CNT represent a possible health risk due to their that did not cause pulmonary inflammation in mice [14]. (cdc.gov)
  • Following initial vacuolization and liquefaction of the lens cortex first observed at postnatal day three, posterior lens rupture occurs in all AND-34 −/− mice, beginning as early as three weeks and seen in all mice at three months. (molvis.org)
  • Indexes of systolic and/or diastolic cardiac function were also abnormal in KO mice at E13.5 and E17.5, in postnatal mice studied at P1, and in KO mice surviving to P28. (mcmaster.ca)
  • We conclude that Fgl2 deficiency is not associated with obvious structural cardiac defects but is associated with a high incidence of neonatal death as well as contractile dysfunction and rhythm abnormalities during embryonic and postnatal development in mice. (mcmaster.ca)
  • Substrate reduction reduces gangliosides in postnatal cerebrum-brainstem and cerebellum in GM1 gangliosidosis mice. (wikigenes.org)
  • In this study, we investigated the effects of N-butyldeoxygalacto-nojirimycin (N B-DGJ), an imino sugar that inhibits ganglioside biosynthesis, in normal C57BL/6J mice and in beta-gal knockout (beta-gal-/-) mice from postnatal day 9 (p-9) to p-15. (wikigenes.org)
  • In addition, the expression of Hat1 is linked to the normal aging process as Hat1 mRNA and protein becomes undetectable in many tissues in old mice. (nih.gov)
  • Activation of D5 receptors is shown to promote expression of brain-derived neurotrophic factor and increase phosphorylation of protein kinase B in rat and mice prefrontal cortex neurons. (wikipedia.org)
  • Synaptic function and plasticity were studied in mice lacking the fragile X mental retardation protein (FMRP), a model for the fragile X mental retardation syndrome. (jneurosci.org)
  • Here we report a role for the phylogenetically ancient mitogen-activated protein kinase (MAPK) signalling pathway in mouse sex determination. (plos.org)
  • We hypothesized that cardiac dysfunction was responsible for the high perinatal lethality that we previously reported in fibrinogen-like protein 2 ( Fgl2) knockout (KO) mice. (mcmaster.ca)
  • Herein, chronic inflammation was induced by intraperitoneal injection of pristane into mice. (hindawi.com)
  • Therefore, we conclude that AT attenuates the progression of liver macrovesicular steatosis and inflammation through AMPK-PPAR-α signaling and PPAR-γ activation, respectively, improving insulin resistance in obese mice. (bvsalud.org)
  • Recent findings show that encountered in the workplace include toluene diisocya- sensitized mice develop airway hyperreactivity and inflammation, nate (TDI), diphenyl-methane diisocyanate and hexam- reflective of human disease. (cdc.gov)
  • Serum from both WT and MMP9-/- mice exhibited inflammatory bioactivity, however, suggesting that MMP9-cleaved ligands may drive vasodilatory impairments, but not endothelial inflammatory responses. (cdc.gov)
  • Finally, we found that infusion of AngII caused a more severe inflammatory response in AMPKα2 knockout mouse aortas, all of which were suppressed by chronic administration of fludarabine. (diabetesjournals.org)
  • When these C57BL/6N-based JM8 cells were injected into blastocysts from albino mice, a high proportion of chimeras displayed ES cell contribution to both germline and somatic tissues. (umassmed.edu)
  • We show that in a macrophageless mouse embryo, null for the haemopoetic-lineage-specific transcription factor, PU.1, the task of phagocytosis is taken over by 'stand-in' mesenchymal neighbours in a clear example of cell redundancy. (bris.ac.uk)
  • We identified a mouse mutant that causes embryonic gonadal sex reversal: the development of ovaries in an XY embryo. (plos.org)
  • Dullard/Ctdnep1 modulates WNT signalling activity for the formation of primordial germ cells in the mouse embryo. (jax.org)
  • Isolation of novel tissue-specific genes from cDNA libraries representing the individual tissue constituents of the gastrulating mouse embryo. (nih.gov)
  • Cyagen subdivides different mouse models into applied research fields and provides detailed information about the backgrounds and associated applications of the models to help simplify the model selection process. (cyagen.com)
  • Furthermore, we generated a mouse model of FSH elevation by intraperitoneally injecting exogenous FSH into ovariectomized (OVX) mice, in which a normal level of estrogen (E2) was maintained by exogenous supplementation. (nature.com)
  • Consistently, the results indicate that FSH, independent of estrogen, increases the serum cholesterol level in this mouse model. (nature.com)
  • For most lines, the mouse ES cells used in generating the model are of the 129S5 strain. (taconic.com)
  • In CKD patients or obstructive mice model, we observed that Plk1, a critical mitotic regulator, was upregulated in kidneys. (nature.com)
  • CONCLUSIONS: Thus, the C57BL/6 strain represents the first Ctns(-/-) mouse model to show clear renal defects. (cnrs.fr)
  • our data support a model in which LRRK2 and PPM1H mutant mice show altered responses to critical Hedgehog signals in the nigrostriatal pathway. (elifesciences.org)
  • In the present study, we investigated the role of TLR2 during the development of experimental deep dermatophytosis in normal mice and mice with alloxan-induced diabetes mellitus, an experimental model of diabetes that exhibits a delay in the clearance of the dermatophyte, Trichophyton mentagrophytes (Tm). (frontiersin.org)
  • The mouse model of diabetes-accelerated atherosclerosis was induced by streptozotocin injections and an atherogenic diet. (hindawi.com)
  • Among them, kenpaullone, an inhibitor of multiple kinases, including cyclin-dependent kinase 2 (CDK2), protected zebrafish lateral-line neuromasts from cisplatin toxicity and, when delivered locally, protected adult mice and rats against cisplatin- and noise-induced hearing loss. (nih.gov)
  • Depending upon the study, these mice often have to be backcrossed 9 more generations with C57BL/6 mice to place the mutant allele on an inbred C57BL/6 background. (umassmed.edu)
  • Of course, one could breed the chimeras with inbred C57BL/6N mice to maintain a pure C57BL/6N substrain background- but then one could not use coat color in the G1 generation to identify germline-transmitting chimeras, as all the offspring would be of a black coat color regardless of germline transmission. (umassmed.edu)
  • post-menopausal women with a higher serum FSH (≥78.3 IU/L) had higher serum TC and LDL-C levels than those with relatively lower FSH levels (40-78.3 IU/L), and ovariectomized mice had higher serum FSH and lipid levels and reduced hepatic LDL receptor expression. (nature.com)
  • Acute 10microg MWCNT exposure resulted in a 90% increase in serum TSP-1 in WT mice compared to DM controls. (cdc.gov)
  • The transfer of lymphocytes or ethylene di-isocyanate (HDI), which are used in a serum from sensitized mice can cause clinical disease in naive variety of industries including polyurethane foam mice. (cdc.gov)
  • The depletion of myeloid-derived suppressor cells (MDSCs) has no impact on mouse breast cancer growth. (frontiersin.org)
  • Here, we analysed the distribution of GABA B receptors using highly sensitive SDS-digested freeze-fracture replica labelling in mouse cerebellar Purkinje cells. (springer.com)
  • iGal-8KO mice displayed anincreased development of splenomegaly (evaluated by cellularity and weight) compared to iWT.Spleen cells analysis by Flow-cytometry showed no percentage differences among CD4 Tlymphocytes, myeloid cells and their different subpopulations, between iGal-8KO and iWT. (conicet.gov.ar)
  • however, TLR2 −/− mice showed a noteworthy increase in production of IFN-γ, IL-10, and IL-17, and an increased percentage of splenic CD25 + Foxp3 + Treg cells. (frontiersin.org)
  • Likewise, p16/p21 double knockout mice, which have very few senescent cells, showed similar delays in wound closure. (jax.org)
  • To understand how senescent cells promote repair, the Campisi team surveyed wound sites in GCV-treated p16-3MR mice and found fewer endothelial cells and fibroblasts, including myofibroblasts, which are contractile cells responsible for wound closure. (jax.org)
  • This is because 129-derived ES cells have proven to be very robust in generating chimeras, with the agouti (129-derived) coloration easily visible on the black (C57BL/6-derived) coat in the chimeras. (umassmed.edu)
  • Furthermore, 129-strain ES cells often contribute to the germline of the chimeras, and when breeding chimeras with C57BL/6 test mice, the dominant agouti color in offspring signifying germline transmission is also easily detected in the G1 generation litters. (umassmed.edu)
  • Recently, researchers from the Sanger Institute (UK) have isolated stable and germline competent embryonic stem (ES) cells from C57BL/6N mice (1). (umassmed.edu)
  • However, one still has to genotype all of the G1 generation mice from germline transmitting chimeras or risk losing some black colored mice that are ES cells derived. (umassmed.edu)
  • Mice were inoculated subcutaneously with Lewis lung carcinoma cells. (bvsalud.org)
  • The viral epitopes targeted by CD4 and CD8 T cells were defined in C57BL/6 mice, and tetramers for both CD4 and CD8 T cells were generated. (cdc.gov)
  • Hat1 +/- mouse embryonic fibroblasts (MEFs) display modest increases in endogenous DNA damage but have significantly higher levels of reactive oxygen species (ROS). (nih.gov)
  • The present study showed that Polo-like kinase 1 (Plk1) was upregulated in the kidneys of CKD patients and mice subjected to unilateral ureteral obstruction (UUO) with location in proximal tubules and tubulointerstitial fibroblasts. (nature.com)
  • Interestingly, PDGF-AA applied topically to GCV-treated mice increased myofibroblast numbers and rescued wound closure rates. (jax.org)
  • Here, we employed MPO knockout genotoxic effects [7] possibly associated with carcinogenesis (e.g. (cdc.gov)
  • Therefore, the aim of this study was to analyze the effect of 8-weeks AT in non-alcoholic liver disease (NAFLD) outcomes in obese mice. (bvsalud.org)
  • Input-output curves and paired-pulse plasticity were not significantly altered in KO compared with WT mice in either the olfactory cortex or hippocampus. (jneurosci.org)
  • Postsynaptic responses to patterned burst stimulation in KO mice showing impaired LTP were not significantly different from those in WT mice, suggesting that the LTP deficit was not caused by alterations in circuit properties. (jneurosci.org)
  • RESULTS: C57BL/6 Ctns(-/-) mice showed significantly higher renal cystine levels than the FVB/N strain. (cnrs.fr)
  • N B-DGJ significantly reduced total ganglioside and GM1 content in cerebrum-brainstem (C-BS) and in cerebellum of normal and beta-gal-/- mice. (wikigenes.org)
  • These mice have reduced glycogen stores in adipose tissue, liver, heart, and skeletal muscle, corresponding with decreased glycogen synthase activity and glycogen synthesis rate. (jci.org)
  • Our results demonstrated that inoculation of Tm into the footpads of normal mice increases the expression of TLR2 in CD115 + Ly6C high blood monocytes and, in hypoinsulinemic-hyperglycemic (HH) mice infected with Tm, the increased expression of TLR2 was exacerbated. (frontiersin.org)
  • At 15 to 25 weeks of age, the homozygote variants of these mice were found to be dramatically susceptible to the development of multiple tumor types, and malignant lymphoma and sarcomas were frequently observed in the thymus and major visceral organs of these animals [ 9 ]. (biomedcentral.com)
  • Alterations of their tumorigenic phenotypes including survival rate, tumor formation and tumor spectrum, were assessed in FVB/N-Trp53 em2Hwl /Korl and C57BL/6-Trp53 em1Hwl /Korl knockout (KO) mice over 16 weeks. (biomedcentral.com)
  • The most frequently observed tumor in both the Trp53 KO mice was malignant lymphoma, while soft tissue teratomas and hemangiosarcomas were only detected in the FVB/N-Trp53 em2Hwl /Korl KO mice. (biomedcentral.com)
  • To investigate the impact of different exercise training schedules (following a fixed schedule or at random times of the day) on clock genes and myokine expression patterns in the skeletal muscle of tumor-bearing mice. (bvsalud.org)
  • Mice were divided into three groups: tumor (LLC), tumor + exercise training (LLC + T) always performed at the same time of the day (ZT2) and exercise training at random times of the day (ZTAlt). (bvsalud.org)
  • A small percentage of AND-34 −/− mice show distinctive small white eye lesions resulting from the migration of ruptured cortical lens tissue into the anterior chamber. (molvis.org)
  • In conclusion, GGTase-I knockout efficiently blocked diabetes-accelerated atherosclerosis, and this protective effect must be related to the inhibition of VSMC proliferation. (hindawi.com)
  • These data demonstrate that MAP3K4-dependent signalling events are required for normal expression of Sry during testis development, and create a novel entry point into the molecular and cellular mechanisms underlying sex determination in mice and disorders of sexual development in humans. (plos.org)
  • To understand the role of TLR2 during the development of murine experimental deep dermatophytosis, we employed TLR2 knockout mice. (frontiersin.org)
  • Fmr1 KO mice exhibit hyperactivity in open field tests, heightened seizure susceptibility, exaggerated responses to sensory stimulation, normal fear conditioning, and mild impairments in spatial learning tasks ( Kooy, 2003 ). (jneurosci.org)
  • 1994. Ah receptor in embryonic mouse palate and effects of TCDD on receptor expression. (cdc.gov)
  • Previously, we showed that cholinergic interneurons of the dorsal striatum lose their cilia in R1441C LRRK2 mutant mice (Dhekne et al. (elifesciences.org)
  • Although their wounds eventually healed, GCV treated mice had substantial fibrotic tissue at the injury site. (jax.org)