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Coxiella burnetiiQ FeverCoxiellaGoat DiseasesSequence Analysis, DNAAntibodies, BacterialDNA, BacterialGoatsSpecies SpecificityRNA, Ribosomal, 16SVirulenceSheep DiseasesPolymerase Chain ReactionGenes, BacterialBacterial Typing TechniquesEscherichia coliMice, Inbred StrainsDNA, RibosomalAnti-Bacterial AgentsEnvironmental MicrobiologyAntigens, BacterialMicrobial Sensitivity Tests
CoxiellaInfectionEscherichiaFeverBacteriaClostridiumBacteriumGenotypesIsolatesCharacterizationAntibodiesBacterialNine MilePrevalencePathogenChaffeensisGenomeVirulentVirulenceHighly infectiousToxinTomato strATCCNeisseriaGoatHepatitisDose-dependentMilkAerosolPregnantEpidemicCloselySpecificSequenceAgentPresentStudyFoundCells
Coxiella44
  • Coxiella burnetii is a strict intracellular bacterium with potential as a bioterrorism agent. (cdc.gov)
  • Coxiella burnetii is a strict intracellular microorganism, included in the γ subdivision of the Proteobacteria phylum ( 1 ). (cdc.gov)
  • citation needed] Coxiella burnetii is also a coccobacillus. (wikipedia.org)
  • Q fever is a zoonotic disease with acute and chronic stages caused by the rickettsia-like organism Coxiella burnetii. (cdc.gov)
  • This area has more details on infection with Coxiella burnetii / Q Fever which is an important zoonotic disease with a wide geographical distribution. (interchem.ie)
  • Coxiella burnetii is an obligate intracellular gram-negative bacterium. (interchem.ie)
  • Schematic outcomes of an intrauterine infection with Coxiella burnetii in a pregnant animal. (interchem.ie)
  • Q fever (infection with Coxiella burnetii ) has been uncommon in Australia's Northern Territory, with no reported cases until 2002. (health.gov.au)
  • Documented cases of infection with Coxiella burnetii (Q fever), a notifiable zoonotic disease, have been uncommon in the Northern Territory. (health.gov.au)
  • Coxiella burnetii is an obligate intracellular bacterium that causes the human disease Q fever, which can manifest as an acute flu-like illness or a long-term chronic illness, such as endocarditis. (cdc.gov)
  • Three genotypes (ST8, ST16, and ST20) of Coxiella burnetii are commonly found in the contemporary US and are associated with specific animal hosts. (cdc.gov)
  • Q fever is a disease caused by the bacterial pathogen Coxiella burnetii. (cdc.gov)
  • Coxiella burnetii, the causative agent of Q fever, is widely present in dairy products around the world. (cdc.gov)
  • Khademi P, Ownagh A, Mardani K, Khalili M. PCR-RFLP of Coxiella burnetii Plasmids Isolated from Raw Milk Samples in Iran. (ijmm.ir)
  • Several methods have been employed to identify Coxiella burnetii isolates based on the specific Coxiella burnetii QpH1 plasmid to distinguish the acute form from the chronic form of Q fever disease in humans and animals owing to the presence of unique gene sequences in this plasmid. (ijmm.ir)
  • A total of 86 isolates of Coxiella burnetii QpH1 plasmid, which were confirmed by the Nested-PCR method in 2018, were used to determine the RFLP panel of the QpH1 plasmid. (ijmm.ir)
  • The results of the nucleic acid sequencing of all 4 samples indicated that they had a Coxiella burnetii type (Nine Mile RSA493 strain). (ijmm.ir)
  • RFLP patterns exhibited no difference on the Coxiella burnetii QpH1 plasmid isolated from cow and buffalo milk. (ijmm.ir)
  • Hence, all isolates were genetically identical, and the infection in animals could originate from one Coxiella burnetii strain (Nine Mile RSA493 strain). (ijmm.ir)
  • Genotyping of Coxiella burnetii in sheep and goat abortion samples. (ijmm.ir)
  • 2. Sobotta K, Hillarius K, Jiménez PH, Kerner K, Heydel C, Menge C. Interaction of Coxiella burnetii Strains of Different Sources and Genotypes with Bovine and Human Monocyte-Derived Macrophages. (ijmm.ir)
  • 3. Hendrix LR, Samuel JE, Mallavia LP. Differentiation of Coxiella burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE. (ijmm.ir)
  • 4. Heinzen R, Stiegler G, Whiting L, Schmitt S, Mallavia L, Frazier M. Use of Pulsed Field Gel Electrophoresis to Differentiate Coxiella burnetii Strains a. (ijmm.ir)
  • 5. Willems H, Ritter M, Jäger C, Thiele D. Plasmid-homologous sequences in the chromosome of plasmidless Coxiella burnetii Scurry Q217. (ijmm.ir)
  • 6. Savinelli EA, Mallavia LP. Comparison of Coxiella burnetii plasmids to homologous chromosomal sequences present in a plasmidless endocarditis-causing isolate. (ijmm.ir)
  • 7. Jäger C, Lautenschläger S, Willems H, Baljer G. Coxiella burnetii plasmid types QpDG and QpH1 are closely related and likely identical. (ijmm.ir)
  • Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing. (ijmm.ir)
  • Establishment of a genotyping scheme for Coxiella burnetii. (ijmm.ir)
  • Scholars@Duke publication: Lipid A Has Significance for Optimal Growth of Coxiella burnetii in Macrophage-Like THP-1 Cells and to a Lesser Extent in Axenic Media and Non-phagocytic Cells. (duke.edu)
  • Here we report the characterization of the role of lipid A in Coxiella burnetii growth in axenic media, monkey kidney cells (BGMK and Vero), and macrophage-like THP-1 cells by using a potent LpxC inhibitor -LPC-011. (duke.edu)
  • In conclusion, our data suggest that lipid A has significance for optimal development of Coxiella-containing vacuoles, and for robust multiplication of C. burnetii in macrophage-like THP-1 cells. (duke.edu)
  • Q fever (see the image below) is a zoonosis caused by Coxiella burnetii, an obligate gram-negative intracellular bacterium. (medscape.com)
  • PCR, immunohistochemistry, or culture of affected tissue can provide definitive confirmation of infection by Coxiella burnetii. (medscape.com)
  • Study of the distribution of Coxiella burnetii infection in small ruminant farms in the Balearic Islands by taking environmental samples. (neiker.eus)
  • Goats are known reservoirs of Coxiella burnetii, the etiologic agent of Q fever. (biomedcentral.com)
  • Coxiella burnetii , an obligate intracellular pathogen of both humans and animals is the causative agent of Q-fever [ 1 ]. (biomedcentral.com)
  • Q fever bacterium common in U.S. An environmental study determined that Coxiella burnetii , the causative agent of Q fever - a bacterial infection that can affect the lungs, liver, heart, and other parts of the body - is fairly common in the United States, suggesting that exposure to the bacterium may be more frequent than what is suggested by the number of reported cases - fewer than 200 annually. (mlo-online.com)
  • One of these ticks, Rhipicephalus rossicus, which is reported occasionally from humans, seems to be particularly interesting because of its demonstrated vectorial role for zoonotic pathogens like Francisella tularensis, Coxiella burnetii, or CCHF and West Nile viruses. (nih.gov)
  • Because Marburg has a high case fatality rate of up to 90%, Soviet scientists preferred it to Coxiella burnetii (Q fever). (kignasu.com)
  • Coxiella burnetii, bacteria that cause Q fever. (umn.edu)
  • The bacterium Coxiella burnetii (which causes Q fever) had the highest incidence because of a goat-related Q fever epidemic in the Netherlands from 2007 to 2019, peaking at 2,424 cases in 2009. (umn.edu)
  • Microbes which have been shown to trigger CFS/ME include enteroviruses, Epstein-Barr virus, Chlamydia pneumoniae , parvovirus B19, Coxiella burnetii , Borna disease virus, varicella zoster virus, cytomegalovirus, and human herpesvirus type 6 (HHV-6). (bmj.com)
  • Recent success in the cultivation of Tropheryma whipplei [4] (which causes Wipple's disease) and Coxiella burnetii [5] (which causes Q fever) using genome-based metabolic pathway analyses has renewed interest in formulating axenic growth media for M. leprae . (internationaltextbookofleprosy.org)
  • First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a uncared for meals security hazard in ready-to-eat raw-milk product Background: World publications on Q fever have elevated after the 2007 epidemic within the Netherlands. (atto-gentaur.com)
Infection6
  • ST16 and ST20 isolates induced robust antibody and cytokine responses, while ST8 isolates produced significantly lower anti-C. burnetii titers early in the infection but saw increased titers in some animals several weeks post-infection. (cdc.gov)
  • This study indicates that seroprevalence of C. burnetii in Boer goats raised in Missouri was low, but it does not preclude the existence of a higher level of infection in Missouri's meat goat herds. (biomedcentral.com)
  • More research is warranted to corroborate the preliminary findings reported here in order to determine the public health significance C. burnetii infection risks associated with contemporary goat production systems in the US. (biomedcentral.com)
  • A review of the burden of Q fever in the US revealed a 41.6% average prevalence of C. burnetii infection in goats [ 9 ]. (biomedcentral.com)
  • According to the hospital, the suspicion of the novel strain of infection first came to notice in February 2010, but two patients have recovered and the third is still being treated for an unrelated illness. (mlo-online.com)
  • It is a fatal disease caused by infection with one of the Ebola virus strains transmitted to humans by wild animals. (kignasu.com)
Escherichia2
Fever4
  • Although macrolides and quinolones have some reported efficacy against C. burnetii, 2 patients experienced prolonged fever (5 and 9 days respectively) despite azithromycin, and the fatality occurred in a patient treated with multiple antibiotics including ciprofloxacin. (health.gov.au)
  • These results may provide some basis for why ingestion of C. burnetii as a mode of Q fever transmission is not often reported. (cdc.gov)
  • Although all three genotypes have been isolated from humans with Q fever, studies comparing virulence between C. burnetii sequence types have been rare. (cdc.gov)
  • Loftis and others detected C. burnetii in 42.9% (9/21) of commercial raw milk samples in the US [ 7 ], and a recent case report found Q fever clusters among raw milk consumers in the State of Michigan [ 4 ]. (biomedcentral.com)
Bacteria2
  • Unlike many bacteria, C. burnetii replication in axenic media and non-phagocytic cells was less dependent on normal lipid A biosynthesis. (duke.edu)
  • There is evidence that C. burnetii is a public health hazard in the US with humans being exposed to the bacteria through milk. (biomedcentral.com)
Clostridium1
  • A virulent strain of Clostridium difficile ( C diff ) has been detected in Australia for the first time. (mlo-online.com)
Bacterium1
  • Another example of diplococcus strain is Moraxella catarrhalis that is a gram-negative bacterium that causes infections of the respiratory system . (bioexplorer.net)
Genotypes1
  • Strain characterisation will contribute to the elaboration of a map of the distribution of C. burnetii genotypes in the eastern part of Spain, which is less studied in these aspects. (neiker.eus)
Isolates4
  • To characterize different isolates of C . burnetii at the molecular level, we performed multispacer sequence typing (MST). (cdc.gov)
  • These spacers were then tested in 159 additional isolates obtained from different geographic areas or different hosts or were implicated in different manifestations of human disease caused by C . burnetii . (cdc.gov)
  • Previous studies have shown that C . burnetii isolates differed respect to their plasmid type (QpH1, QpRS, QpDG, and QpDV) ( 3 - 6 ), lipopolysaccharide profiles ( 7 ), and analysis of endonuclease-digested DNA separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis ( 8 ) or pulsed-field gel electrophoresis (PFGE) ( 9 - 11 ). (cdc.gov)
  • We investigated the utility of multispacer sequence typing (MST) with 173 C . burnetii isolates. (cdc.gov)
Characterization1
  • Considering the fact that some subpopulation of the F. tularensis strains is more virulent, more significantly associated with mortality, and therefore poses more threat to humans, rapid identification and characterization of this subpopulation strains is of invaluable importance. (openmicrobiologyjournal.com)
Antibodies2
  • Banked serum samples were secondarily tested for C. burnetii specific antibodies. (biomedcentral.com)
  • paratuberculosis (MAP) in Missouri Boer goat herds were secondarily tested for C. burnetii specific antibodies [ 14 ]. (biomedcentral.com)
Bacterial1
  • MST allows easy comparison and exchange of results obtained in different laboratories and could be a useful tool for identifying bacterial strains. (cdc.gov)
Nine Mile1
  • Recently, the whole genome of the C . burnetii Nine Mile strain was sequenced ( 14 ). (cdc.gov)
Prevalence4
  • Given the high prevalence of C. burnetii in dairy products, we sought to examine the feasibility of transmitting the major sequence types (ST16, ST8 and ST20) of C. burnetii circulating in the United States. (cdc.gov)
  • However, there has been very little research on the prevalence of C. burnetii exposure and risk in meat goats farmed in the US. (biomedcentral.com)
  • No studies have evaluated the prevalence and risk factors of C. burnetii infections in meat goats farmed in Missouri. (biomedcentral.com)
  • It is not known whether these three cases are linked because the prevalence of this strain in the wider Australian community is unknown due to lack of testing. (mlo-online.com)
Pathogen1
  • however, C. burnetii is not believed to be a major gastrointestinal pathogen. (cdc.gov)
Chaffeensis2
  • Ehrlichia chaffeensis str. (yeastrc.org)
  • Bifunctional protein FolD OS=Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas) GN=folD PE=3 S. (yeastrc.org)
Genome1
  • Differentiation was also obtained by sequence determination of the isocitrate dehydrogenase gene ( 12 ), com1 gene, and mucZ gene, which was renamed djlA when the whole genome of C . burnetii was sequenced ( 13 , 14 ). (cdc.gov)
Virulent2
  • USA100 - a healthcare-associated MRSA strain - was the most common MRSA strain detected in patients, but the more virulent community-associated strain USA300 was more commonly found in HIV-infected patients, researchers found. (mlo-online.com)
  • A particularly highly virulent strain of this bug named the "Quebec strain" has previously affected thousands of people in Britain, North America, and Europe over the last 10 years. (mlo-online.com)
Virulence1
Highly infectious2
  • C . burnetii is highly infectious by the aerosol route and can survive for long periods in the environment. (cdc.gov)
  • National laboratories are select laboratories, such as CDC and USAMRIID, that have unique resources to handle highly infectious agents and to identify specific strains of an agent. (cdc.gov)
Toxin2
  • An entity may request the exclusion of an attenuated strain of a select agent or a select toxin modified to be less potent or toxic. (cdc.gov)
  • The Intragovernmental Select Agents and Toxins Technical Advisory Committee (ISATTAC) reviews the exclusion request by conducting a risk assessment to determine whether the attenuated strain or modified toxin has the potential to pose a severe risk to public health and safety. (cdc.gov)
Tomato str1
ATCC6
  • All the strains were propagated on Vero cell monolayers (ATCC CRL 1587). (cdc.gov)
  • Bifunctional protein FolD 1 OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) GN=fol. (yeastrc.org)
  • Bifunctional protein FolD OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2. (yeastrc.org)
  • Bifunctional protein FolD 1 OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=folD1 PE=3 S. (yeastrc.org)
  • Bifunctional protein FolD OS=Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) GN=f. (yeastrc.org)
  • Bifunctional protein FolD 1/3 OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) GN=folD1. (yeastrc.org)
Neisseria1
  • One example of a diplococci strain is Neisseria gonorrhea . (bioexplorer.net)
Goat1
  • The aim of this pilot investigation was to determine whether Boer goats, the preponderant meat goat breed in Missouri, were exposed to C. burnetii and to estimate seroprevalence of the exposure. (biomedcentral.com)
Hepatitis1
  • Chronic microbial infections which have been documented in patients with CFS/ME include C burnetii , parvovirus B19, C pneumoniae , hepatitis C, enteroviruses and human retroviruses. (bmj.com)
Dose-dependent1
  • Despite being able to induce an immune response in a dose-dependent manner, administration of C. burnetii via OG is the least efficient route tested. (cdc.gov)
Milk1
  • A recent report found that 94% of bulk tank milk samples collected from US dairy herds contained C. burnetii specific DNA [ 6 ]. (biomedcentral.com)
Aerosol1
  • Therefore, we delivered three strains of C. burnetii (representing three pertinent sequence types in the United States, such as ST16, ST20, and ST8) to immunocompetent mice in four doses via aerosol challenge (AC), intraperitoneal injection (IP), or OG. (cdc.gov)
Pregnant1
  • While infected ruminants remain largely asymptomatic, the primary signs of C. burnetii include spontaneous, late-term abortions in pregnant animals. (biomedcentral.com)
Epidemic1
  • The Quebec strain got its name from an epidemic there in 2003 that caused 7,000 people to fall ill and killed nearly 1,300. (mlo-online.com)
Closely1
  • Officials said successful immunization campaigns would hinge on authorized vaccines that closely match circulating strains alongside robust surveillance strategies. (umn.edu)
Specific1
  • notice with recommendations to add or remove specific BSATs or exclude a subset of BSAT strains or serotypes from the select agent list. (cdc.gov)
Sequence1
  • TSA: Nitella hyalina strain KGK0190 nhya_c13900_c mRNA sequence. (nig.ac.jp)
Agent2
  • As of December 2017, DSAT has excluded 50 strains of biological agents and 10 modified toxins from the select agent regulations. (cdc.gov)
  • The joint analysis of the characterisation data and the environmental setting in which each variant of C. burnetii develops will make it possible to describe epidemiological aspects of great interest, both in terms of the host-microorganism relationship and the natural cycle of this agent. (neiker.eus)
Present1
  • This result is inconclusive because this study was disadvantaged by the small number of individual animal and herds tested, which compromised the statistical power of this study to detect a possible higher seroprevalence of C. burnetii in this population, if present. (biomedcentral.com)
Study1
  • The C . burnetii strains included in this study are listed in Table A1 . (cdc.gov)
Found2
  • C. burnetii is found in every country except New Zealand, 1 and in multiple animal hosts including wild and domestic mammals, birds and ticks. (health.gov.au)
  • We found that regularly changing inhibitor-containing media was required for sustained inhibition of C. burnetii LpxC in cells. (duke.edu)
Cells1
  • Infected cells were maintained in a 5% CO 2 atmosphere at 35°C. C . burnetii cells were harvested, pelleted, resuspended in 200 μL MEM, and mixed with 500 μL Chelex 100 20% (Bio-Rad, Ivry sur Seine, France). (cdc.gov)