• Burkholderia pseudomallei , the causative agent of melioidosis is associated with soil. (cambridge.org)
  • Burkholderia pseudomallei is a Gram-negative, biothreat pathogen and the causative agent of melioidosis, an often fatal infectious disease for humans. (biomedcentral.com)
  • Melioidosis is a bacterial infection caused by Burkholderia pseudomallei (formerly known as Pseudomonas pseudomallei or Whitmore's bacillus), which is found in many tropical regions of the world ( 1 ). (cdc.gov)
  • Burkholderia pseudomallei (also known as Pseudomonas pseudomallei) is a Gram-negative, bipolar, aerobic, motile rod-shaped bacterium. (wikipedia.org)
  • Even when the isolate is recognized to be significant, commonly used identification systems may misidentify the organism as Chromobacterium violaceum or other nonfermenting, Gram-negative bacilli such as Burkholderia cepacia or Pseudomonas aeruginosa. (wikipedia.org)
  • Melioidosis is an infection caused by the gram-negative bacterium Burkholderia (formerly Pseudomonas ) pseudomallei . (msdmanuals.com)
  • Although it is mainly a soil-dwelling bacteria, a study performed by Apinya Pumpuang and others showed that Burkholderia pseudomallei survived in distilled water for 16 years, demonstrating that it is capable of living in water if a specific environment is provided. (wikipedia.org)
  • B. pseudomallei isolates from both patients and the environmental samples were all genetically similar and were distinct from previous known isolates, indicating bacteria from the environment was the likely source of infection for both patients and has been present in the area since at least 2020. (cdc.gov)
  • Melioidosis is an infectious disease caused by the bacteria Burkholderia pseudomallei . (cdc.gov)
  • Infections with bacteria of the genus BURKHOLDERIA . (lookformedical.com)
  • Chien (2021, Nov). Characterization of two chromate reducing bacteria isolated from heavy metal contaminated soil. (ncu.edu.tw)
  • These strains had been isolated from dairy product imports into China from 9 countries between 2005 and 6. (biomedcentral.com)
  • We compared the genome sequences of two strains of B. pseudomallei: the original reference strain K96243 from Thailand and strain MSHR305 from Australia. (edu.au)
  • These alternate genomic states define two distinct groups within B. pseudomallei: all strains contained either the BTFC gene cluster (group BTFC) or the YLF gene cluster (group YLF). (edu.au)
  • Most Burkholderia pseudomallei strains will readily kill mice, whereas B. thailandensis strains do not. (cdc.gov)
  • But one of the things that we found in our study is that the Burkholderia thailandensis strains that we do know about from the United States are very diverse--that is, when you compare them, they have a lot of differences in their DNA. (cdc.gov)
  • Real-time, live-cell imaging of B. pseudomallei strains demonstrated dynamic morphological changes in broth containing clinically relevant β-lactam antibiotics. (biomedcentral.com)
  • For all B. pseudomallei strains evaluated, increased exposure time and exposure to increased concentrations of AMC at and above minimal inhibitory concentrations (MICs) in broth resulted in cell morphology shifts from filaments to spheroplasts and/or cell lysis. (biomedcentral.com)
  • Both resistant and susceptible B. pseudomallei strains exhibited filamentation during early exposure to AMC and CAZ at concentrations used to interpret susceptibility (based on CLSI guidelines). (biomedcentral.com)
  • B. pseudomallei was identified through environmental sampling of soil and water in the Gulf Coast region of southern Mississippi during an investigation of two human melioidosis cases. (cdc.gov)
  • Burkholderia pseudomalle is a gram-negative bacterium and Tier 1 Select Agent that typically lives in soil and water in regions with tropical and subtropical climates around the world, such as South and Southeast Asia, northern Australia, parts of Central and South America, the U.S. Virgin Islands, and Puerto Rico. (cdc.gov)
  • David Wagner] Burkholderia thailandensis is a gram-negative bacterium that lives in the soil and water, and sometimes can be an opportunistic pathogen in humans. (cdc.gov)
  • B . pseudomallei dwells in soil and water, and is transmitted via percutaneous inoculation, inhalation, or the ingestion of infected food or water. (ophrp.org)
  • It has been isolated from soil and water in tropical regions, particularly Southeast Asia. (lookformedical.com)
  • Identification of B. pseudomallei and all occupational exposures must be reported to the Federal Select Agent Program immediately (i.e., within 24 hours), whereas states are not required to notify CDC's Bacterial Special Pathogens Branch (BSPB) of human infections. (cdc.gov)
  • David Wagner] Infections with Burkholderia thailandensis are much less serious, both in mice and humans. (cdc.gov)
  • So we actually know very little about human infections with Burkholderia thailandensis , but given how common it can be in the environment in some parts of the world, it likely does not cause serious infections in people exposed to it under normal circumstances. (cdc.gov)
  • RÉSUMÉ La présente étude visait à estimer la prévalence et les facteurs de risque des infections liées aux procédures de soins dans tous les hôpitaux de la région du centre-est de la Tunisie, comptant neuf établissements, en 2005. (who.int)
  • Nous en concluons que les infections liées aux procédures de soins constituent un motif de préoccupation dans cette région de Tunisie. (who.int)
  • Naturally-acquired melioidosis infections are caused by inhalation, ingestion or exposure of broken skin to the pathogen Burkholderia pseudomallei . (biomedcentral.com)
  • A federal investigator who visited the Tulane National Primate Research Center (TNPRC) in late January as part of the response to the November 2014 Burkholderia pseudomallei infections of two macaques who may have been exposed to the bacterium. (outbreaknewstoday.com)
  • While poultry constitutes the major industry in Nepal, there is a paucity of evidence on colistin resistance in Escherichia coli isolates causing natural infections in poultry. (tropmedres.ac)
  • This study aimed to explore the prevalence of plasmid-mediated colistin resistance gene, mcr-1 in E. coli isolated from liver samples of dead poultry suspected of E. coli infections. (tropmedres.ac)
  • For environmental specimens only, differentiation from the nonpathogenic B. thailandensis using an arabinose test is necessary (B. thailandensis is never isolated from clinical specimens). (wikipedia.org)
  • This presentation will explain the role of laboratory professionals in detecting B. pseudomallei in clinical specimens and how to plan for possible exposure cases. (cdc.gov)
  • Of the 261 persons at risk for occupational exposure to B. pseudomallei while performing laboratory diagnostics, 43 (16%) persons had high-risk exposures, 130 (50%) persons had low-risk exposures, and 88 (34%) persons were classified as having undetermined or unknown risk. (cdc.gov)
  • While developing a rapid β-lactam antimicrobial susceptibility test based on cell-shape alone requires more extensive analyses, optical microscopy detected B. pseudomallei growth attributes that lend insight into antibiotic response and antibacterial mechanisms of action. (biomedcentral.com)
  • The results demonstrated that these isolates represent a rare and unusual, novel Burkholderia species for which we propose the name B. singularis. (edu.au)
  • Its genome sequence has an average mol% G+C content of 64.34%, which is considerably lower than that of other Burkholderia species. (edu.au)
  • B. singularis can be differentiated from other Burkholderia species by multilocus sequence analysis, MALDI-TOF mass spectrometry and a distinctive biochemical profile that includes the absence of nitrate reduction, a mucoid appearance on Columbia sheep blood agar, and a slowly positive oxidase reaction. (edu.au)
  • Comparisons with publicly available whole genome sequences demonstrated that strain TSV85, an Australian water isolate, also represents the same species and therefore, to date, B. singularis has been recovered from human or environmental samples on three continents. (edu.au)
  • In 2021, an intraoperative periaortic swab sample was confirmed positive for a Burkholderia species, and three of the thirty clinical staff-identified exposures were confirmed laboratory-acquired exposures. (cdc.gov)
  • Of all of the Burkholderia species described to date, none are more closely related to Burkholderia pseudomallei than Burkholderia thailandensis . (cdc.gov)
  • Of course, there could be more closely related species that just haven't been described yet, but from what we know so far, B. thailandensis is the most closely related organism to Burkholderia pseudomallei . (cdc.gov)
  • MALDI-TOF MS analysis of Burkholderia pseudomallei and closely related species isolated from soils and water in Khon Kaen, Thailand. (kku.ac.th)
  • B. pseudomallei infection has been identified in humans and multiple animal species. (cdc.gov)
  • Transmission may be horizontal and vertical, and as Brucella species have been isolated from lungworm and can survive in fish, ingestion may also be an important mode of transmission. (msdvetmanual.com)
  • Here, considering the high phylogenetic conservation within Burkholderia species, we ask whether cross reactivity can be reciprocally displayed by the synthetic epitope from B. cenocepacia. (unimi.it)
  • We perform comparative analyses of the conformational preferences and diagnostic performances of the corresponding epitopes from the two Burkholderia species when presented in the context of the full-length proteins or as isolated peptides. (unimi.it)
  • Multiple-locus VNTR analysis revealed extensive diversity within the global isolate set containing B. pseudomallei and B. mallei, and it detected genotypic differences within clonal lineages of both species that were identical using previous typing methods. (nau.edu)
  • Comparative genomics of Burkholderia singularis sp. (edu.au)
  • We used large-scale comparative genomics to assess the genetic diversity, phylogeography and potential origins of B. pseudomallei in Myanmar. (ox.ac.uk)
  • The classic textbook description of B. pseudomallei in clinical samples is of an intracellular, bipolar-staining, Gram-negative rod, but this is of little value in identifying the organism from clinical samples. (wikipedia.org)
  • Melioidosis is an infectious disease caused by the soil organism Burkholderia pseudomallei . (cambridge.org)
  • 1,2 A recent model estimated an incidence of 165,000 melioidosis cases per year (an incidence rate of 5.0 per 100,000 people at risk), with a predicted mortality of 89,000 per year, among the 3 billion people residing in areas likely to contain B. pseudomallei . (health.mil)
  • 3 The true global distribution of B. pseudomallei and the incidence of melioidosis remain poorly understood, and it is not yet known if the growing number of melioidosis cases reported worldwide reflects an unmasking of long-standing bacterial presence or the spread of B. pseudomallei to previously unaffected areas. (health.mil)
  • With the increased presence of B. pseudomallei and Melioidosis cases, laboratory workers are at an increased risk of exposure. (cdc.gov)
  • Thus, a systematic literature search was performed using PubMed and the Korean Medical Database, with the following keywords "melioidosis" and " Burkholderia pseudomallei " to identify melioidosis cases that occurred before 2011. (ophrp.org)
  • Four Burkholderia pseudomallei-like isolates of human clinical origin were examined by a polyphasic taxonomic approach that included comparative whole genome analyses. (edu.au)
  • We present the first whole-genome sequences of B. pseudomallei isolates from Myanmar: nine clinical and seven environmental isolates. (ox.ac.uk)
  • An infection would be counted as a new infection if a person is culture-positive within an 18-month time period with an isolate that is distinct from the previous infection by whole genome sequencing. (cdc.gov)
  • This region was previously identified in comparisons of the genome of B. pseudomallei strain K96243 with the genome of strain E264 from the closely related B. thailandensis. (edu.au)
  • Here, we show that the homologous genomic region in B. pseudomallei strain 305 is similar to that previously identified in B. thailandensis strain E264. (edu.au)
  • We have named this region in B. pseudomallei strain 305 the B. thailandensis-like flagellum and chemotaxis (BTFC) gene cluster. (edu.au)
  • We'll be discussing the detection of Burkholderia thailandensis in the environment in the United States. (cdc.gov)
  • Sarah Gregory] What is Burkholderia thailandensis ? (cdc.gov)
  • Sarah Gregory] And how serious is the B. thailandensis infection compared to the B. pseudomallei infection? (cdc.gov)
  • David Wagner] Burkholderia thailandensis is usually found in Southeast Asia and the tropical northern end of Australia. (cdc.gov)
  • Most people, including us, do not go out and look for Burkholderia thailandensis on its own, but rather, we find it and isolate it when we're out looking for Burkholderia pseudomallei . (cdc.gov)
  • Given that the global distribution of Burkholderia pseudomallei itself is still pretty poorly understood, I'd say that we know almost nothing about the global distribution of Burkholderia thailandensis . (cdc.gov)
  • So that suggests that Burkholderia thailandensis has been in the United States for a long time and it has had a lot of time to persist in local locations, hence all the DNA differences among different isolates from different locations in the United States. (cdc.gov)
  • Burkholderia thailandensis is what we would call an opportunistic pathogen. (cdc.gov)
  • We then screened 104 of the potentially polymorphic loci across a diverse panel of 31 isolates including B. pseudomallei, B. mallei and B. thailandensis in order to identify loci with varying degrees of polymorphism. (nau.edu)
  • B. pseudomallei, amongst other pathogens, has been found in monkeys imported into the United States from Asia for laboratory use, posing a risk that the pathogen could be introduced into the country. (wikipedia.org)
  • Conclusion: The high prevalence of mcr-1 in colistin-resistant E. coli isolates in our study is a cause of concern for the probable coming emergence of colistin resistance in human pathogens, due to horizontal transfer of resistant genes from poultry to human isolates. (tropmedres.ac)
  • A study published yesterday in Emerging Infectious Diseases finds low a incidence of pathogens with bioterrorism potential in the Netherlands, which the authors say would hamper efforts to isolate them from natural sources for intentional release. (umn.edu)
  • We identified and categorized tandem repeat arrays and their distribution throughout the genome of B. pseudomallei strain K96243 in order to develop a genetic typing method for B. pseudomallei. (nau.edu)
  • B. pseudomallei appears genetically di- virus in domestic small ruminants in southern Gabon. (cdc.gov)
  • ABSTRACT A 21-year survey conducted in northeast Thailand of antimicrobial resistance to parenteral antimicrobial drugs used to treat melioidosis identified 24/4,021 (0.6%) patients with one or more isolates resistant to ceftazidime ( n = 8), amoxicillin-clavulanic acid ( n = 4), or both drugs ( n = 12). (tropmedres.ac)
  • abstract = "Burkholderia pseudomallei is the etioiogic agent of melioidosis. (edu.au)
  • We screened for these different genomic components across additional genome sequences and 571 B. pseudomallei DNA extracts obtained from regions of endemicity. (edu.au)
  • Recent genomic studies showed that B. pseudomallei originated in Australia and spread to Asia, with phylogenetic evidence of repeated reintroduction of B. pseudomallei across countries bordered by the Mekong River and the Malay Peninsula. (ox.ac.uk)
  • The frequency, location and duplicate nature of tandemly repeated regions within the B. pseudomallei genome indicate that these tandem repeat regions may play a role in generating and maintaining adaptive genomic variation. (nau.edu)
  • Coxiella burnetii antibodies in bulk milk from cattle, sheep, cases as sporadic, isolated events in a few geographic and goats in Jordan. (cdc.gov)
  • In an effort to assess the risk of B. pseudomallei infection to service personnel in Australia, 341 paired samples, representing pre- and post-deployment samples of Marines who trained in Australia, were analyzed for antibodies against B. pseudomallei antigens. (health.mil)
  • Burkholderia pseudomallei was isolated from the psoas abscess pus cultures and the diagnosis of melioidosis was confirmed with high titers of Burkholderia pseudomallei antibodies and positive PCR. (biomedcentral.com)
  • Our study provides new insights into global patterns of B. pseudomallei dissemination, most notably the dynamic nature of movement of B. pseudomallei within densely populated Southeast Asia. (ox.ac.uk)
  • The role of anthropogenic influences in both ancient and more recent dissemination of B. pseudomallei to Myanmar and elsewhere in Southeast Asia and globally requires further study. (ox.ac.uk)
  • Burkholderia pseudomallei is not fastidious and grows on a large variety of culture media (blood agar, MacConkey agar, EMB, etc. (wikipedia.org)
  • Burkholderia pseudomallei grown on sheep blood agar for 96 hours. (health.mil)
  • modeling suggests that the environmental conditions found in the Gulf Coast states are conducive to the growth of B. pseudomallei [1]. (cdc.gov)
  • A fixed-interval sampling strategy was used as the basis for detection and quantitation by culture of B. pseudomallei in soil in two environmental sites (disused land covered with low-lying scrub and rice field) in northeast Thailand. (plos.org)
  • We discuss the implications of the uneven distribution of B. pseudomallei in soil for future environmental studies, and describe a range of established geostatistical sampling approaches that would be suitable for the study of B. pseudomallei that take account of our findings. (plos.org)
  • In addition, clinical isolates are more likely to belong to group YLF, whereas environmental isolates are more likely to belong to group BTFC. (edu.au)
  • The Department of Health and Human Services' Centers for Disease Control and Prevention, along with the U. S. Department of Agriculture (USDA) and the Environmental Protection Agency (EPA), continues to work with Tulane University and state and local officials to identify, isolate, mitigate and prevent further transmission of BURKHOLDERIA PSEUDOMALLEI within the compound. (outbreaknewstoday.com)
  • Given the health threat to humans and livestock and the potential for B. pseudomallei to be released intentionally, MLVA could prove to be an important tool for fine-scale epidemiological or forensic tracking of this increasingly important environmental pathogen. (nau.edu)
  • Unusual flora -Burkholderia pseudomallei (occasionally reported in melioidosis), actinomycotic and mycobacterial abscesses, most typically seen in immunosuppressed patients. (medscape.com)
  • Burkholderia pseudomallei, the causative agent of the potentially fatal tropical disease melioidosis, is known to be highly resistant to oxidative stress although the mechanism of this resistance remains to be fully elucidated. (dna-pksignal.com)
  • The median B. pseudomallei cfu/gram from positive sampling points was 378 and 700 for the disused land and the rice field, respectively (p = 0.17). (plos.org)
  • B. pseudomallei can be identified in exudates by methylene blue or Gram stain and by culture. (msdmanuals.com)
  • Burkholderia pseudomallei (Bp) is a saprophytic, gram-negative aerobe and the causative agent of the disease melioidosis. (bepress.com)
  • Detection of Plasmid-Mediated Colistin Resistant mcr-1 Gene in Escherichia coli Isolated from Infected Chicken Livers in Nepal. (tropmedres.ac)
  • Five patients had been notified with no confirmatory isolate received. (ox.ac.uk)
  • The federal select agent program designates B. pseudomallei as a Tier 1 overlap select agent, which can affect both humans and animals. (cdc.gov)
  • And Burkholderia pseudomallei can cause very serious disease in humans, even death, especially in immunocompromised people. (cdc.gov)
  • Global phylogenetics demonstrated that Myanmar isolates group in two distantly related clades that reside in a more ancestral Asian clade with high amounts of genetic diversity. (ox.ac.uk)
  • The diversity of B. pseudomallei from Myanmar and divergence within our global phylogeny suggest that the original introduction of B. pseudomallei to Myanmar was not a recent event. (ox.ac.uk)
  • Across geographically diverse B. pseudomallei and B.mallei isolates, the 32 VNTR loci displayed between 7 and 28 alleles, with Nei's diversity values ranging from 0.47 and 0.94. (nau.edu)
  • Additionally, we will study the metagenomics of the household water supply for both patients and controls, including the presence of B. pseudomallei. (ox.ac.uk)
  • and 3) all possible occupational exposures to B. pseudomallei be reported voluntarily to BSPB. (cdc.gov)
  • Again, because the disease is rarely seen in Western countries, identification of B. pseudomallei in cultures may not actually trigger alarms in physicians unfamiliar with the disease. (wikipedia.org)
  • This comprehensive approach will allow us to identify B. pseudomallei and their paired host genetic factors associated with disease acquisition and severity. (ox.ac.uk)
  • The United States Federal Select Agent Program includes B. pseudomallei as a Tier 1 biological select agent. (biomedcentral.com)
  • 40 UK cases were confirmed as B. pseudomallei by the reference laboratory. (ox.ac.uk)
  • Clinical presentation is extremely variable and can range from acute septicemia with bacterial dissemination to distant sites, to an isolated pulmonary infection. (bepress.com)
  • Soil sampling was performed using a fixed-interval grid of 100 sampling points in each of two sites (disused land and rice field) in northeast Thailand, and the presence and amount of B. pseudomallei determined using culture. (plos.org)
  • In AST, 95.1% (137/144) and 82.6% (119/144) of E. coli isolates were resistant against tetracycline and ciprofloxacin, respectively, while 13.2% (19/144) and 25.7% (37/144) isolates were resistant to cefoxitin and imipenem, respectively. (tropmedres.ac)
  • In the same assay, 76.4% (110/144) E. coli isolates were multi-drug resistant (MDR). (tropmedres.ac)
  • In the PCR assay, 43.9% (18/41) of colistin-resistant isolates were screened positive for plasmid-mediated mcr-1. (tropmedres.ac)
  • Analysis of pre- and post-deployment serum samples obtained from the Department of Defense Serum Repository identified serological evidence of possible infection with B. pseudomallei in U.S. Marines who trained in Australia during 2012-2014. (health.mil)
  • Here, we identified β-lactam targets in B. pseudomallei by in silico analysis. (biomedcentral.com)
  • A subset of these tandem repeat arrays were subsequently developed into a multiple-locus VNTR analysis to examine 66 B. pseudomallei and 21 B. mallei isolates from around the world, as well as 95 lineages from a serial transfer experiment encompassing ∼18,000 generations. (nau.edu)
  • General Information: This strain was a clinical isolate from Thailand. (up.ac.za)
  • Blood cultures were positive for B. pseudomallei, and both patients recovered following appropriate antibiotic therapy. (cdc.gov)
  • To address this hypothesis, we aim to collect, sequence, and analyse genetic data from melioidosis patients and controls, along with isolates of B. pseudomallei obtained from patients. (ox.ac.uk)
  • Additionally, we are isolating B. pseudomallei from the melioidosis patients to obtain bacterial DNA. (ox.ac.uk)
  • Patients have to be isolated from mosquitoes so as to avoid spread of the infection. (targetwoman.com)