Borrelia mayonii was identified as a sensu lato (broad sense), genospecies (Bbsl) of the known microbe, Borrelia burgdorferi. (wikipedia.org)
Two isolates, gene strains, of B. mayonii, named MN14-1420 and MN14-1539, were sequenced, process of arranging a chain of genetic information to match up to a specific organism's genetic code, and compared to the genome or genetic material of Borrelia burgdorferi. (wikipedia.org)
Borrelia mayonii is a bacterial genospecies discovered in the Midwestern United States by Pritt and colleagues at the Mayo Clinic in Minnesota during routine polymerase chain reaction (PCR) of the oppA1 gene of B. burgdorferi in 2016. (wikipedia.org)
The Borrelia genus members have a linear chromosome which is about 900 kbp in length as well as an excess of both linear and circular plasmids in the 5-220 kbp size range. (prospecbio.com)
The Lyme disease spirochete, Borrelia burgdorferi , is transmitted by the slow-feeding ixodid tick Ixodes scapularis, whereas the relapsing fever spirochete, B. hermsii , is transmitted by Ornithodoros hermsi , a fast-feeding argasid tick. (cdc.gov)
B. burgdorferi , fever a causative agent of Lyme disease, and B. hermsii , a causative a Borrelia burgdorferi, B. garinii, and B. afzelii are known human pathogens. (cdc.gov)
Китове за Real Time PCR Бактерии и Паразити BOR SL- Kit for the detection of Borrelia burgdorferi sensu lato. (elta90.com)
Thereafter, I obtained my current position in Dr. Bankhead's lab, and am currently investigating the mechanisms of immune evasion and persistent infection by Borrelia burgdorferi. (bankheadlab.org)
Wong JK, Crowley MA, Bankhead T. Deletion of a Genetic Region of lp17 Affects Plasmid Copy Number in Borrelia burgdorferi. (bankheadlab.org)
Lone AG and Bankhead T. The Borrelia burgdorferi VlsE lipoprotein prevents antibody binding to an arthritis-related surface antigen. (bankheadlab.org)
Lyme disease is a multisystemic disorder caused by tick-borne infection of humans or other mammalian hosts with Borrelia burgdorferi. (uth.edu)
The present study investigated the prevalence of Borrelia burgdorferi sensu lato (s.l.) genospecies in Ixodes ricinus ticks collected in Hanover, Northern Germany, in 2010. (biomedcentral.com)
Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi. (cdc.gov)
Borrelia Elisa Laboratories manufactures the burrelia burgdorferi abtibody elisa 5.08 reagents distributed by Genprice. (mooneylab.com)
This manuscript offers an image presentation of diverse forms of Borrelia burgdorferi spirochetes which are not spiral or corkscrew shaped. (canlyme.com)
In America, this nine months' shift between MS and schizophrenic births is also reflected by the periodicity of Borrelia burgdorferi transmitting Ixodes pacificus ticks along the West Coast and the periodicity of Ixodes scapularis along the East Coast. (biomedcentral.com)
2007. p38 mitogen-activates protein kinase controls NF-kB transcriptional activation and tumor necrosis factor alpha production through RelA phosphorylation mediated by mitogen-and stress activated protein kinase 1 in response to Borrelia burgdorferi antigens. (charlotte.edu)
2006. Control of Borrelia burgdorferi -specific CD4 + T cell effector function by interleukin-12- and T-cell receptor-induced p38 mitogen-activated protein kinase activity. (charlotte.edu)
2005. Distinct bacterial dissemination and disease outcome in mice subcutaneously infected with Borrelia burgdorferi in the midline of the back and the footpad. (charlotte.edu)
The antigens of Borrelia burgdorferi can be separated according to their molecular weight by means of SDS-Page (laboratory method for the separation of proteins) and determined by a size marker. (altmeyers.org)
Borrelia burgdorferi has the ability of adhesion, invasion, persistence and immune evasion. (altmeyers.org)
During the infection Borrelia burgdorferi migrate into different tissues of the host organism to reach so-called "immune privileged niches" where they are protected from the attack of the immune system. (altmeyers.org)
In addition, some experts make the assumption that Borrelia burgdorferi sensu lato is not the only factor to explain this disease, in particular certain persistent forms as the persistent polymorphic syndrome possibly due to a tick bite (SPPT), officially recognized by the French High Authority for Health (HAS) (a governmental institution https://www.has-sante.fr ), and the post-treatment Lyme disease syndrome (PTLDS) (1). (fortuneonline.org)
Lyme disease is caused by the bacterium Borrelia burgdorferi, which is transmitted to humans through the bite of infected black-legged ticks. (rnpedia.com)
Spread by Ticks Caused by bacteria (borrelia) (Borrelia Burgdorferi) It causes borreliosis (lymes disease) The disease is multisystemic - involvement of skin, heart, joints and CNS Affects mostly young and middle-aged people. (slideserve.com)
Borrelia burgdorferi strain B31 A3 is an auto player crossfire clonal derivative of the type strain B31 Burgdorfer et al. (feiqizhili.cn)
Several accounts of Borrelia burgdorferi L-forms (also referred to as cysts or round-bodies) were observed after starvation conditions [31, 32], in which serum-minus media and water were each used for induction. (camkkinases.com)
Lyme4
In this study, we have identified and characterized an elaborate genetic system in the Lyme disease spirochete B. burgdorferi that promotes extensive antigenic variation of a 34-kDa surface-exposed lipoprotein, VlsE. (uth.edu)
B. burgdorferi sensu stricto resides in the cerebrospinal fluid of mammals in Europe and the United States and is most often associated with Lyme arthritis. (mooneylab.com)
Complement inhibitor factor H binding to Lyme disease spirochetes is mediated by inducible expression of multiple plasmid-encoded outer surface protein E paralogs. (ouhsc.edu)
Lyme arthritis, for example, occurs mainly in infections with B. burgdorferi s. s. (altmeyers.org)
Genospecies2
Additional B. burgdorferi sensu lato genospecies are suspected of causing illness, but are not confirmed by culture. (newworldencyclopedia.org)
Especially within the human pathogenic genospecies (B. burgdorferi s. s. (altmeyers.org)
Spirochete1
The spirochete travels from the tick mid-gut during tick feeding, to the tick salivary glands and into the mammal host, and it is believed that this migration is facilitated by changes in expression of different B. burgdorferi genes. (thenativeantigencompany.com)
Spirochetes3
To do so, we performed next-generation RNA sequencing on doxycycline-treated spirochetes and treated spirochetes following regrowth, comparing them to untreated B. burgdorferi . (frontiersin.org)
Detailed studies of B. burgdorferi were initiated in 1982 used by tick-borne spirochetes to adapt for transmission by when Burgdorfer and coworkers reported these bacteria in their tick vectors, may lead to unique disease prevention strat- adult Ixodes scapularis ticks collected from vegetation on egies. (cdc.gov)
The life transmission cycle of B. burgdorferi requires migration of spirochetes from tick's gut to its salivary glands during vertebrate's blood sucking, penetrating to the vertebrate's tissues and their colonization. (aaem.pl)
Genes5
Our goal was to examine gene transcription by B. burgdorferi following doxycycline treatment in an effort to identify both persister-associated genes and possible targets for antimicrobial intervention. (frontiersin.org)
Genes upregulated in the treated B. burgdorferi included a number of Erp genes and rplU , a 50S ribosomal protein. (frontiersin.org)
Studies are underway to determine if these same genes are perturbed in B. burgdorferi treated with doxycycline in a host environment. (frontiersin.org)
The plasmids are atypical, as compared to most bacterial plasmids, since they contain many paralogous sequences, a large number of pseudogenes and, in some cases, essential genes. (prospecbio.com)
Garinii3
B. burgdorferi sensu stricto (predominant in North America, but also in Europe), B. afzelii, and B. garinii (both predominant in Eurasia). (newworldencyclopedia.org)
At present, diagnostic tests are based only on B. burgdorferi sensu stricto (the only species present in the United States), B. afzelii, and B. garinii. (newworldencyclopedia.org)
Like B. garinii, B. burgdorferi is a trigger of neuroborreliosis. (altmeyers.org)
Including plasmid transfers2
Genetic exchange, including plasmid transfers, contributes to the pathogenicity of the organism. (newworldencyclopedia.org)
B. burgdorferi sensu stricto commonly undergoes genome-wide genetic exchange, including plasmid transfers. (mooneylab.com)
Ticks5
We describe how B. burgdorferi and B. hermsii change their outer surface during their alternating infections in ticks and mammals, which in turn suggests biological functions for a few surface-exposed lipoproteins. (cdc.gov)
Simultaneously, results obtained in 2010 were compared to infection rates from 2005 to evaluate the development of B. burgdorferi s.l. infection rates in Hanoverian ticks. (biomedcentral.com)
Overall, 22.7% (476/2,100) of collected ticks were tested positive for B. burgdorferi s.l. infections. (biomedcentral.com)
Comparing B. burgdorferi s.l . infections in Hanoverian I. ricinus ticks in 2010 with data from 2005, a statistically significant increase of infected larvae was noted, whereas the other stages revealed no statistically significant differences. (biomedcentral.com)
Transmission of B. burgdorferi s.l. from ticks to hosts may occur through different developmental stages of I. ricinus, as transstadial transmission is very efficient. (biomedcentral.com)
Protein4
The vls locus with the vlsE site, as it is present in strain B. burgdorferi B31, encodes a lipoprotein, a fatty protein, that allows B. burgdorferi to infect various mammalian hosts. (wikipedia.org)
However, the isolates lack various genomic regions seen in B. burgdorferi such as the region that codes for CspZ (complement inhibitor) and BB_K32 (fibronectin binding protein). (wikipedia.org)
A 28-kilobase linear plasmid of B. burgdorferi B31 (lp28-1) was found to contain a vmp-like sequence (vls) locus that closely resembles the variable major protein (vmp) system for antigenic variation of relapsing fever organisms. (uth.edu)
B. burgdorferi sensu stricto has a linear plasmid (lp54) which carries a two-gene operon encoding two surface lipoproteins, DbpA and DbpB, both of which bind Decorin Binding Protein A (DbpA). (thenativeantigencompany.com)
Genome2
One of the most striking features of B. burgdorferi as compared with other bacteria is its unusual genome, which is far more complex than that of its spirochetal cousin Treponema pallidum, the agent of syphilis. (newworldencyclopedia.org)
[9] The genome of B. burgdorferi includes a linear chromosome approximately one megabase in size, and 21 plasmids (12 linear and 9 circular)-the largest number of plasmids (double-stranded DNA molecules separate from the chromosomal DNA ) found in any known bacterium [10] . (newworldencyclopedia.org)
Pathogenicity1
This frequent recombination implies a potential for rapid adaptive evolution as well as a possible polygenic basis of B. burgdorferi sensu stricto pathogenicity. (mooneylab.com)
VlsE2
B. burgdorferi has 15 silent cassettes with a vlsE expression site. (wikipedia.org)
This combinatorial form of antigenic variation could potentially yield millions of VlsE variants in the mammalian host, and thereby contribute to immune evasion, long-term survival, and pathogenesis of B. burgdorferi. (uth.edu)
Chromosomally encoded1
2004. Effects of temperature on detection of plasmid or chromosomally encoded gfp - and lux- labeled Pseudomonas fluorescens in soil. (charlotte.edu)
Isolates1
Isolates showing evidence for plasmid-chromosome recombination. (cdc.gov)
Phenotype2
In the set of experiments described in this report, two keys aspects of doxycycline-treated B. burgdorferi phenotype were evaluated. (frontiersin.org)
The presence of lp28-1 correlates with the high-infectivity phenotype in B. burgdorferi strains tested. (uth.edu)
Detection1
Detection of recA amplicon of B. burgdorferi in the presence of human genomic DNA in a multiplex real-time PCR assay We had PRIMA-1MET manufacturer already optimized molecular beacons and PCR conditions for quantitative detection of B. burgdorferi DNA by real-time PCR [61]. (pkc-inhibitors.com)
Proteins2
[11] Long-term culture of B. burgdorferi results in a loss of some plasmids and changes in expressed proteins. (newworldencyclopedia.org)
The location of DbpA and DbpB in the outer membrane of B. burgdorferi allows exposure of these proteins to the host immune system. (thenativeantigencompany.com)
OspA1
OspA serotype 1 corresponds to the species B. burgdorferi s.s. (altmeyers.org)
Amplicon1
If the sequence result was of low quality, the amplicon was cloned into a plasmid vector using a TOPO TA cloning kit (Invitrogen, Carlsbad, California) and then sequenced using the primers provided with the kit. (who.int)
Recombination1
These unusual properties of borreliae are associated with large number of plasmids, which show a high variability as a result of recombination with each other. (aaem.pl)
Elisa1
The Burrelia Burgdorferi Abtibody Elisa 5.08 reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (mooneylab.com)
Chromosome1
It has a linear chromosome with an additional 15 plasmids, eight linear and seven circular. (wikipedia.org)
Molecular mechanisms1
Analysis of the results of expression studies of the luxS gene shows that the molecular mechanisms of this phenomenon in B. burgdorferi are only fragmentarily known. (aaem.pl)
Immune evasion1
2009). Another mechanism of immune evasion is the ability of B. burgdorferi to bind host-specific complement regulators (Reconectin/FHL-1 and Factor H) to the cell surface. (altmeyers.org)
Commonly1
Resistance to β-lactams is commonly due to cells containing plasmid encoded β-lactamases. (toku-e.com)