HealthGene Fast RNA and DNA Elution Kit™ provides high quality and easy-to-use reagents for nucleic acids elution from polyacrylamide gels in the shortest possible time. (healthgene.com)
Gradient Elution1
In contrast to RP-LC, gradient elution HILIC begins with a low-polarity organic solvent and elutes polar analytes by increasing the polar aqueous content [ 13 ]. (springer.com)
Lysis14
The tissue then undergoes an incubation in lysis buffer, tissue homogenization, a 30 second sonication followed by digestion with Enzymatic Shearing Cocktail. (activemotif.com)
Bring all three buffers (Lysis/Binding, Wash and Elution Buffers) to a final concentration of 8M Urea or 6M Guanidine. (neb.com)
When the recommended protocol is followed, each isolation requires the following volumes of buffer: 0.75 ml of Lysis/Binding Buffer, 0.75 ml of Wash Buffer and 0.4 ml of Elution Buffer. (neb.com)
Crude lysate should be prepared with a lysis buffer without imidazole. (neb.com)
Resuspend cell pellet in Lysis Buffer and lyse using method of choice (use approximately 5 ml of lysis buffer per gram of cell paste) . (neb.com)
It is recommended that imidazole be omitted from any lysis buffer. (neb.com)
Add 250 μl of Lysis/Binding buffer to the column. (neb.com)
Centrifuge column at 800 x g for 1 minute, discard the Lysis/Binding buffer. (neb.com)
RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. (thermofisher.com)
HOOK™ Cell Surface Protein Isolation Kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. (gbiosciences.com)
Resuspend the cells in 25 milliliters of lysis buffer, pre-chill to four degrees Celsius, containing one EDTA-free protease inhibitor tablet. (jove.com)
Next, equilibrate a gravity flow column containing 10ml of immobilized cobalt affinity resin with lysis buffer. (jove.com)
Then, wash the resin twice with 100ml portions of lysis buffer. (jove.com)
After lysis, apply cell lysate to a column in the presence of a high salt buffer allowing nucleic acid and nucleic acid binding proteins to adsorb to the matrix. (cdc.gov)
Phosphate Buffer1
The entire collected sample is eluted into 0.2 or 1 mL of sterile phosphate-buffered saline (PBS) using a customized particle removal system made of glass. (cdc.gov)
Tris-HCl1
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer. (novusbio.com)
Nucleic3
New RNA and DNA Elution Kit™ for fast extraction nucleic acids form polyacrylamide gels. (healthgene.com)
Apply a wash buffer to the same column to wash out contaminants in order to improve purity of the eluted nucleic acid. (cdc.gov)
Finally, apply an appropriate elution buffer of water to release the nucleic acid from the solid matrix into a collecting container. (cdc.gov)
Resuspend1
After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline, containing 0.08% sodium azide for storage. (vectorlabs.com)
NaCl2
For convenience and consistency we provide Streptavidin Binding Buffer (20mM NaPO4, 0.15M NaCl (pH7.5) and Streptavidin Elution Buffer (8M Guanidine.HCl pH1.5). (gbiosciences.com)
2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. (vectorlabs.com)
Wash5
To further minimize contaminants in the eluate, the concentration of imidazole in the wash buffer can be increased to 10 mM (≥10 mM can reduce the isolated yield but may result in increased purity). (neb.com)
The QIAGEN Plasmid Plus Kits also feature a dedicated wash buffer for endotoxin reduction. (qiagen.com)
The endotoxin wash buffer provided in the kits reduces bacterial endotoxins to levels usually below 1 EU/μg DNA. (qiagen.com)
Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. (vectorlabs.com)
4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. (vectorlabs.com)
Proteins3
Gentle Elution Buffer allows for elution of CL7-tagged proteins from Im7 resin and/or regeneration of Im7 resin without denaturing effects. (trialtusbioscience.com)
Unbound proteins are collected in the flow-through and/or in subsequent buffer washes. (wikipedia.org)
Pass a buffer containing a low amount of chaotropic salt through the column to remove these proteins. (cdc.gov)
Resin2
The high salt and near-neutral pH of Gentle Elution Buffer maintains the native structure of the target protein and the Im7 resin ligand without need for subsequent refolding steps. (trialtusbioscience.com)
8 If the adsorption of the protein to the resin is of concern, the resin can be included in a dialysis buffer and the protein dialyzed. (sigmaaldrich.com)
Protein4
After it settles, the column is pre-equilibrated in buffer before the protein mixture is applied. (wikipedia.org)
Both of these techniques can displace the negatively charged protein which is then eluted into test tubes fractions with the buffer. (wikipedia.org)
Run a 15mcl aliquot of the eluere on a four to 15%SDS-PAGE gel to confirm the elution of the protein. (jove.com)
Purify the concentrated protein on a gel filtration column pre-equilibrated with Buffer A.One of the critical steps in this procedure is ensuring you remove all aggregates during the purification. (jove.com)
Concentration5
First, the salt concentration in the elution buffer is gradually increased. (wikipedia.org)
An excess volume of each concentrated buffer is provided for preparation of cell lysates or to optimize the concentration of imidazole in the washes. (neb.com)
The hemoglobin fractions (A1c, A1b, F, LA1c, SA1c, A0 and H-Var) are subsequently removed from the column material by step-wise elution using elution buffers each with a different salt concentration. (cdc.gov)
The sample elution system was also redesigned and optimized to achieve a practical solution yielding a high sample concentration rate. (cdc.gov)
60%. The use of 0.2 mL elution liquid resulted in a sample concentration rate of approx. (cdc.gov)
Volumes2
Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. (qiagen.com)
High-yield protocols and extra buffer volumes are provided with the QIAGEN Plasmid Plus Kits. (qiagen.com)
Digestion1
Digestion buffer for use with Zymo Research purification kits for DNA and RNA extractions. (thomassci.com)
Bead1
The optimized liquid handling parameters on ASSIST PLUS allow efficient DNA clean-up, without bead or buffer carryover. (integra-biosciences.com)
Salt1
After the removal of contaminants, the purified DNA is eluted with a low salt elution buffer or water. (primelabmed.com)
Product1
0.25x TE Rinse Buffer, 60mL This product is only available to U.S. Domestic Customers. (thomassci.com)
Volume3
Gentle Elution Buffer is also a component of TriAltus' Starter Kit in a 100 mL volume. (trialtusbioscience.com)
Blue Loading Pack ( #7722 ) or Red Loading Pack ( #7723 ) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. (cellsignal.com)
The Select-a-Size MagBeads selectively binds fragments based on the volume of buffer added relative to the sample. (zymoresearch.com)
Column3
Centrifuge column at 800 x g for 1 minute to remove the storage buffer, discard the buffer. (neb.com)
Elute Nsa1 from the column with 20ml of elution buffer. (jove.com)
1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity. (vectorlabs.com)
Reduces1
TE Buffer pH 7.0 reduces base hydrolysis through chelation of divalent cations by EDTA and through resistance to pH changes by the Tris buffer. (genelink.com)
Purification1
Buffer EB is the elution buffer used in the QIAquick PCR , Gel Extraction , Nucleotide Removal Kits , and MinElute Kits for DNA cleanup, and the QIAprep Miniprep Kits for small-scale plasmid purification. (qiagen.com)
Preparation1
All Gene Link TE Buffers are provided nuclease-free, ready for use, and require no extra preparation. (genelink.com)
System2
Its single buffer system can recover DNA from enzymatic reactions, impure extractions, library preparations, and other sources. (integra-biosciences.com)
Complexes of Vps34, the sole class III PI3K member, produce PI 3-phosphate (PI(3)P) in the endolysosomal system and during autophagy to regulate vesicle-mediated sorting en route to lysosomes 1 . (nature.com)
Prepare2
#4050 ) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH 2 O, mix. (cellsignal.com)
#12539 ) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH 2 O, mix. (cellsignal.com)
Solution1
DNA Elution Buffer (Low TE) solution consists of 10 mM Tris pH 8.0 and 0.1 mM EDTA. (genelink.com)