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YeastsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsFungal ProteinsGenes, FungalYeast, DriedMolecular Sequence DataDNA, FungalSchizosaccharomycesAmino Acid SequenceSaccharomycetalesMutationBase SequenceSchizosaccharomyces pombe ProteinsTwo-Hybrid System TechniquesRNA, FungalSequence Homology, Amino AcidCloning, MolecularChromosomes, Artificial, YeastGenome, FungalChromosomes, FungalProtein BindingPlasmidsKiller Factors, YeastRecombinant Fusion ProteinsTranscription FactorsDNA-Binding ProteinsGene DeletionTranscription, GeneticCell Cycle ProteinsSequence AlignmentProtein Structure, TertiaryMitochondriaCandida albicansBinding SitesVacuolesNuclear ProteinsKineticsSpores, FungalCarrier ProteinsTemperatureMutagenesisTransformation, GeneticRecombinant ProteinsMembrane ProteinsRhodotorulaModels, BiologicalFermentationKluyveromycesConserved SequenceRestriction MappingDNA PrimersAdenosine TriphosphatasesCulture MediaEscherichia coliCell NucleusMitosisSuppression, GeneticSpecies SpecificityRNA, MessengerMeiosisSubstrate SpecificityMutagenesis, Site-DirectedDNA, ComplementaryRecombination, GeneticVesicular Transport ProteinsMycologyProtein KinasesProtein TransportBiological TransportAntifungal AgentsDNA ReplicationRNA, RibosomalPromoter Regions, GeneticRNA, TransferModels, MolecularCathepsin AHaploidyRepressor ProteinsGene LibraryMicroscopy, FluorescenceMycological Typing TechniquesYarrowiaProtein-Serine-Threonine KinasesGreen Fluorescent ProteinsTelomereAllelesGenes, Mating Type, FungalRNA-Binding ProteinsProtein Interaction MappingSequence Analysis, DNASequence DeletionCell WallFungiProton-Translocating ATPasesSequence Homology, Nucleic AcidAscomycotaChromosomal Proteins, Non-HistoneProtein BiosynthesisMacromolecular Substances
SaccharomycesOrganismChromosomeSchizosaccharomycesFission yeastsMulticellular and unicellular organismsGenesProteinProteinsHyphaeOrganismsMitotic cell cycleMechanismHomologueCellsChromosomesSpeciesEndocytosisGeneticsMoleculesLocalizationQuantificationPathwayPhysiologyMRNAOrganizationMultiplyRegulationReproduceBiologyFunctionalFunctionsOutgrowthGrewExpressionGeneticModelSynthesizeDetect
Saccharomyces10
  • We have previously shown that budding yeast Mak11, whose homologues in other eukaryotes were described as modulating a p21-activated protein kinase function, accumulates in Rlp24-associated pre-60S complexes when their maturation is impeded in Saccharomyces cerevisiae. (pasteur.fr)
  • Saccharomyces cerevisiae cells lacking the MDM12 gene product display temperature-sensitive growth and possess abnormally large, round mitochondria that are defective for inheritance by daughter buds. (rupress.org)
  • Alkalinization of the medium represents a stress condition for the budding yeast Saccharomyces cerevisiae to which this organism responds with profound remodeling of gene expression. (microbialcell.com)
  • The budding yeast Saccharomyces cerevisiae grows under a relatively broad range of external pH, but proliferates far better at acidic than at neutral or alkaline pH. (microbialcell.com)
  • Bud formation in Saccharomyces cerevisiae and a comparison with the mechanism of cell division in other yeasts. (ulster.ac.uk)
  • The cell cycles of the budding yeast Saccharomyces cerevisiae and the fission yeast, Schizosaccharomyces pombe are currently the best understood of all eukaryotes. (ox.ac.uk)
  • Research in Saccharomyces cerevisiae (budding yeast) has shown that four major deviations from the mitotic cell cycle during meiosis are essential for halving ploidy. (warwick.ac.uk)
  • The budding yeast Saccharomyces cerevisiae was used as eukaryotic reference cell to investigate the impact of αSyn on protein homeostasis and the interplay between αSyn and proteins associated with 26S proteasome. (uni-goettingen.de)
  • We demonstrated that this "generalized" adaptor-tagged competitive PCR (GATC-PCR) can be performed between genomic DNA and cDNA to accurately measure absolute expression level of each mRNA in the budding yeast Saccharomyces cerevisiae. (elsevierpure.com)
  • Saccharomyces cerevisiae (often known as budding yeast, or brewers yeast) is a single-celled micro- organism that is easy to grow and genetically manipulate. (lu.se)
Organism5
  • Our methods can be used to understand the lipid specificities of all yeast SNX-BARs or to produce recombinant SNX-BAR proteins from any other organism. (jove.com)
  • The identification of an actomyosin-based contractile ring in budding yeast has recently established this organism as a general model for studying cytokinesis. (johnshopkins.edu)
  • Budding is a type of asexual reproduction in which a new organism develops from an outgrowth or bud due to cell division at one particular site . (moviecultists.com)
  • Budding, in biology, a form of asexual reproduction in which a new individual develops from some generative anatomical point of the parent organism . (moviecultists.com)
  • The yeast on systemic therapy with steroids or antibi- phase of this organism is classified as P. otics was excluded as well as those treated orbiculare [ 2,3 ]. (who.int)
Chromosome5
  • Complete DNA sequence of yeast chromosome II. (genome.jp)
  • RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts. (crcm-marseille.fr)
  • Our results indicate that in both yeasts, RPA directly facilitates telomerase activity at chromosome ends. (crcm-marseille.fr)
  • To determine how changes in chromosome positioning affect transcription across thousands of genes with minimal perturbation, we characterized nuclear organization and global gene expression in budding yeast containing chromosome fusions. (hal.science)
  • These data suggest that basal transcriptional activity is sensitive to radial changes in gene position, and provide insight into the functional relevance of budding yeast chromosome-level 3D organization in gene expression. (hal.science)
Schizosaccharomyces2
  • Moreover, we found that Skb15, the Mak11 homologue in Schizosaccharomyces pombe, also associated with preribosomes and affected 60S biogenesis in fission yeast. (pasteur.fr)
  • In a new study in the Journal of Agricultural and Food Chemistry , Wang and coworkers were able to toughen up budding yeast against caffeine by adding bfr1 , a gene from Schizosaccharomyces pombe that encodes the ABC transporter that shunts caffeine out of the cell. (yeastgenome.org)
Fission yeasts1
  • Cell cycle molecules and mechanisms of the budding and fission yeasts. (ox.ac.uk)
Multicellular and unicellular organisms2
  • Budding is another form of asexual reproduction seen in both multicellular and unicellular organisms. (pracnow.com)
  • Budding is a kind of asexual reproduction, which is most frequently related in both multicellular and unicellular organisms. (moviecultists.com)
Genes2
  • Reid, J. L., Iyer, V. R., Brown, P. O. & Struhl, K. Coordinate regulation of yeast ribosomal protein genes is associated with targeted recruitment of Esa1 histone acetylase. (nature.com)
  • Furthermore, we constructed a large-scale GATC-PCR system to measure absolute expression levels of 5,038 genes to show that the yeast contains more than 30,000 copies of mRNA molecules per cell. (elsevierpure.com)
Protein6
  • Assuming 4-5 pg of protein from a typical haploid yeast cell and an average protein molecular weight of 50 kDa, this gives a total constituency of ~50 million protein molecules. (harvard.edu)
  • Though endocytic protein abundance in S. pombe and S. cerevisiae are more similar than previously thought, membrane invagination speed and depth are two-fold greater in fission yeast than in budding yeast. (biorxiv.org)
  • The functional inactivation of WD40 repeat protein Mak11 interfered with the 60S rRNA maturation, led to a cell cycle delay in G(1), and blocked green fluorescent protein-tagged Rpl25 in the nucleoli of yeast cells, indicating an early role of Mak11 in ribosome assembly. (pasteur.fr)
  • We show that depletion of Top2 protein from budding yeast cells prevents DNA decatenation during S phase. (figshare.com)
  • Expression of αSyn in yeast results in growth impairment and cytoplasmic protein inclusions resembling the aggregates observed within LBs. A tandem fluorescent protein timer (tFT) was exploited to perform a high-throughput screen for proteins with altered turnover upon expression of αSyn or a phosphorylation-deficient S129A mutant. (uni-goettingen.de)
  • This study corroborates a complex mechanistic interplay between proteasome and pS129 αSyn causing a substantial altered protein homeostasis in yeast as model for PD. (uni-goettingen.de)
Proteins3
  • The data presented here is a result of systematically tracking the localization and abundance of 5330 yeast proteins at single-cell resolution under three different stress conditions (DTT, H 2 O 2 and nitrogen starvation) using the GFP-tagged yeast library. (weizmann.ac.il)
  • Purifying the SNB-BAR proteins from yeast cells allows the acquisition of native homo and hetero SNX-BAR dimers while minimizing the toxicity and insolubility frequently encountered with non-native expression systems. (jove.com)
  • However, it remains unclear if, and how, other proteins /pathways regulate TORC1 in simple eukaryotes like yeast . (bvsalud.org)
Hyphae2
  • •Detect yeast and/or pseudo-hyphae in skin, hair and nail specimens using 40X light microscopy. (slideshare.net)
  • KOH dissolves keratin found in cellular material and freeing fungal hyphae and yeast cells. (slideshare.net)
Organisms4
  • The finding of small yeast in fine-needle aspiration of lymph nodes suggests a variety of yeast and yeast-like organisms as well as yeast mimics. (cap.org)
  • The experiment aims to study the process of reproduction in two different types of organisms - unicellular Amoeba and multicellular Hydra and yeast - using prepared slides. (pracnow.com)
  • 16 .Discuss the significance of binary fission and budding in the growth and reproduction of unicellular and multicellular organisms. (pracnow.com)
  • Overview of Fungal Infections Fungi are eukaryotic organisms that exist as yeast, molds, or both forms. (msdmanuals.com)
Mitotic cell cycle1
  • This chapter provides an overview of our current knowledge of the molecules and mechanisms that regulate the mitotic cell cycle in these two yeasts. (ox.ac.uk)
Mechanism2
  • Genetics and live-cell imaging revealed core CME spatiodynamic similarities between the two yeasts, though two-zone actin assembly is a fission yeast-specific mechanism, which is not essential for CME. (biorxiv.org)
  • Both RNA polymerase I and II (Pol I and Pol II) in budding yeast employ a functionally homologous "torpedo-like" mechanism to promote transcriptional termination. (ox.ac.uk)
Homologue1
  • The yeast casein kinase I homologue Yck2p directly phosphorylates six serine residues located in the C-terminal tail of Ste2p, and mutation of these serine residues to alanine significantly decreased recruitment of Ste2p to CCPs. (elsevierpure.com)
Cells12
  • Begin by inoculating a large swab of yeast cells into a flask at least four times the volume of the culture containing 50 milliliters of standard YP medium supplemented with 2%raffinose and 0.1%glucose as a carbon source and grow the culture overnight in a 30 degree Celsius shaker. (jove.com)
  • The next morning, harvest cells by centrifugation and transfer the yeast pellet into a 50 milliliter conical tube. (jove.com)
  • While not as robust as bfr1-B , this mutant allowed yeast expressing it to tolerate caffeine concentrations up to 15 mg/ml, conditions under which cells with wild type bfr1 failed to grow. (yeastgenome.org)
  • However, single-cyclin cells fail to polarize or grow buds and thus cannot survive. (crick.ac.uk)
  • • Yeast cells stain blue [Gram positive]. (slideshare.net)
  • Examine stain for budding cells to confirm that it is a yeast cell and not an artifact. (slideshare.net)
  • High levels of Rpn14 were deleterious to yeast cells and enhanced αSyn-induced growth retardation. (uni-goettingen.de)
  • 2 .Observe the yeast cells under low magnification to locate the cells undergoing budding. (pracnow.com)
  • Under low magnification, yeast cells appeared as single cells. (pracnow.com)
  • Under high magnification, buds were observed growing on the surface of some cells. (pracnow.com)
  • Note the presence of budding yeast form cells. (cdc.gov)
  • Yeasts consist of solitary cells that reproduce by budding. (msdmanuals.com)
Chromosomes1
  • The deviations are (1) formation of a link between homologous chromosomes by crossover, (2) monopolar attachment of sister kinetochores during meiosis I, (3) protection of centromeric cohesion during meiosis I, and (4) suppression of DNA replication following exit from meiosis I. In this review we present the current understanding of the above four processes in budding yeast and examine the possible conservation of molecular mechanisms from yeast to humans. (warwick.ac.uk)
Species8
  • To gain fundamental mechanistic insights into this process, a side-by-side quantitative comparison of CME was performed on two distantly related yeast species. (biorxiv.org)
  • These studies identified conserved CME mechanisms and species-specific adaptations and have broad implications that extend from yeast to humans. (biorxiv.org)
  • Caloric restriction (CR) has been shown to extend lifespan and prevent cellular senescence in various species ranging from yeast to humans. (molcells.org)
  • If growth occurs - perform proper identification methods: Yeast identification methods Manual and automated biochemical reactions capable of identifying most but not all yeast species. (slideshare.net)
  • Newer methods (1) Mass spectrometry - MALDI-TOF and (2) 16 sRNA sequencing are more precise methods with the expanding taxonomy of yeast species. (slideshare.net)
  • However some species may be budded during the winter while they are dormant. (moviecultists.com)
  • However, a number of metazoan animals (e.g., certain cnidarian species) regularly reproduce by budding. (moviecultists.com)
  • Budding is the joining of two genetically different species of a particular fruit variety through the cohesion of their cambiums . (moviecultists.com)
Endocytosis4
  • In contrast to budding yeast, WASP-mediated actin nucleation activity plays an essential role in fission yeast endocytosis. (biorxiv.org)
  • Both budding and fission yeast have high internal turgor pressure, which strongly opposes plasma membrane invagination necessary for endocytosis. (biorxiv.org)
  • Live-cell super resolution analysis of endocytosis was also performed in fission yeast (Arasada, 2018). (biorxiv.org)
  • We previously used a fluorescently labeled mating pheromone derivative to reveal unappreciated molecular and spatiotemporal features of GPCR endocytosis in budding yeast. (elsevierpure.com)
Genetics2
  • Cell biology approaches, combined with the superb genetics of yeast, have advanced our understanding to CME. (biorxiv.org)
  • Of course unlike even the most grizzled 30 year coffee drinker with ideal genetics, the yeast almost certainly gets no joy from its morning Joe. (yeastgenome.org)
Molecules1
  • In both yeasts, Arp2/3 complex activation drives membrane invagination when triggered by the accumulation of ∼70 WASP molecules. (biorxiv.org)
Localization1
  • Ait1 regulates TORC1 signaling and localization in budding yeast. (bvsalud.org)
Quantification1
  • Absolute quantification of the glycolytic pathway in yeast: deployment of a complete QconCAT approach. (harvard.edu)
Pathway1
  • You can imagine adding the caffeine pathway from coffee to yeast and having the yeast merrily exporting caffeine to the culture medium where it can be harvested. (yeastgenome.org)
Physiology1
  • The budding yeast adapts its physiology to radical external and internal fluctuations in a rapid and robust manner. (weizmann.ac.il)
MRNA1
  • By using DNA microarrays, researchers precisely measured the decay of each yeast mRNA, after thermal inactivation of a temperature-sensitive RNA polymerase II. (harvard.edu)
Organization1
  • Septin Organization and Functions in Budding Yeast. (nih.gov)
Multiply2
Regulation1
  • Using yeast as a model system, we study the molecular mechanisms of telomere length regulation. (lu.se)
Reproduce1
Biology1
  • High-throughput robotic genetic technologies can be used to study the fitness of many thousands of genetic mutant strains of yeast, and the resulting data can be used to identify novel genetic interactions relevant to a target area of biology. (lu.se)
Functional1
  • When expressed in S. cerevisiae , the S. pombe Mdm12p homolog conferred a dominant-negative phenotype of giant mitochondria and aberrant mitochondrial distribution, suggesting partial functional conservation of Mdm12p activity between budding and fission yeast. (rupress.org)
Functions1
  • Myo2p is an essential class V myosin in budding yeast with several identified functions in organelle trafficking and spindle orientation. (elsevierpure.com)
Outgrowth2
  • In this process, a small bud grows on the surface of the parent cell, and as it grows, it forms an outgrowth. (pracnow.com)
  • The buds grew in size and formed an outgrowth. (pracnow.com)
Grew1
  • For example, whereas wild type yeast barely grew with 8 mg/ml caffeine, the engineered yeast did OK. (yeastgenome.org)
Expression1
  • Here, we present a workflow for the expression, purification and liposome binding of SNX-BAR heterodimers in yeast. (jove.com)
Genetic1
  • Maybe this genetic engineered yeast can make the equivalent of a Red Bull and vodka all in one step! (yeastgenome.org)
Model1
  • The yeast mating pheromone receptor Ste2p has been used as a model to investigate mechanisms of signal transduction, modification, and endocytic internalization of GPCRs. (elsevierpure.com)
Synthesize1
  • In the end we have a bullet-proof yeast when it comes to caffeine that should prove useful for anyone who wants yeast to synthesize caffeine for them. (yeastgenome.org)
Detect1
  • detect the age incidence of this yeast. (who.int)