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  • RNAi
  • These results identify a previously unknown class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi. (sciencemag.org)
  • Experiments such as a RNA polymerase II inhibitior and subsequent gene expression of CXorf26 could enlighten potential function as well as a complete knoockout of YPL225W in yeast using methods such as RNAi. (wikipedia.org)
  • genome
  • Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, from the Yale Genome Analysis Center. (factbites.com)
  • In sum, these results suggest that adaptive evolution of gene expression is common in yeast, that regulatory adaptation can occur at the level of entire pathways, and that similar genome-wide scans may be possible in other species, including humans. (pnas.org)
  • Thus, our work defines spatial organization within the budding yeast nucleus, demonstrates the conserved role of genome architecture in regulating DNA replication, and identifies a molecular mechanism specifically regulating interactions between pericentric origins. (pnas.org)
  • Yeast Genome. (wikipedia.org)
  • cells
  • phase, and duration of the budded phase of diverse cohorts of daughter cells born before, during, and after the transitory state. (factbites.com)
  • This manual is therefore an essential resource for all researchers, from graduate level upward, who use budding yeast to explore the intricate workings of cells. (cshlpress.com)
  • Autophagy is a non-selective and bulk intracellular degradation system of eukaryotic cells and is highly conserved from yeast to human. (genome.jp)
  • Yeast cells expressing the pheromone and receptor of the opposite mating type are capable of forming zygotes with cells of their original mating type. (genetics.org)
  • All the cells within a multicellular organism, or any single cell species i.e. yeast, displays a polarized organization necessary for its proliferation, differentiation or physiological function. (wikipedia.org)
  • Yeast cells share many features about cell polarity with other organism like: regulation by intrinsic and extrinsic cues, conserved regulatory molecules such as Cdc42GTPase, and asymmetry of the cytoskeleton. (wikipedia.org)
  • pointed out that such contacts are frequent between closely related yeast cells for two reasons. (wikipedia.org)
  • Another, more recently discovered function of WHI3 is encoding for memory in budding yeast cells. (wikipedia.org)
  • During sexual reproduction, two yeast cells signal their presence by diffusing pheromones, and it has been shown that when a cell is exposed to mating pheromones but does not perform mating, it "remembers" the event and is less likely to undergo mating afterwards. (wikipedia.org)
  • The daughter cells obtained from this budding, however, are not conditioned against mating, unlike the mother: the WHI3 aggregates have been shown to localize within the mother cell. (wikipedia.org)
  • A Super-Assembly of WHI3 Encodes Memory of Deceptive Encounters by Single Cells during Yeast Courtship. (wikipedia.org)
  • replication
  • Herein, we describe the existence of topologically associating domains in budding yeast and show that these domains regulate replication timing so that origins within a domain fire synchronously. (pnas.org)
  • mechanisms
  • In the present article, we provide an overview of the main signalling system and discuss the mechanisms by which yeast integrates and separates signals from these sources. (mendeley.com)
  • computational
  • We show in the budding yeast website and the companion paper that a computational model, built on the consensus picture of regulatory network derived from reductive biochemical studies and genetic manipulations, is largely successful (predicting 120 out of 133 cases) in explaining the phenotypes of currently characterized mutants of the regulatory system. (factbites.com)
  • Growth
  • budding yeast used for bread-making, where the carbon dioxide produced by growth in the dough causes the bread to rise. (factbites.com)
  • Amon has also examined trisomy in the mouse as a model of mammalian cell growth and physiology and demonstrated that mammalian aneuploidy results in a stress response analogous to yeast aneuploidy. (wikipedia.org)
  • model
  • This laboratory manual provides a comprehensive collection of experimental procedures that continue to make budding yeast an informative model. (cshlpress.com)
  • Efficiency
  • In yeast, selection for increased mating efficiency is likely to have continually boosted pheromone levels and the ability to discriminate between partners who make more and less pheromone. (genetics.org)
  • Search
  • The human orthologues of Mad2 (MAD2L1 and MAD2L2) were first cloned in a search for human cDNAs that would rescue the microtubule poison-sensitivity of a yeast strain in which a kinetochore binding protein was missing. (wikipedia.org)
  • describe
  • The contributors describe methods for culturing and genetically modifying yeast, strategies and tools (e.g., gene deletion collections) for functional analyses, approaches for characterizing cell structure and morphology, and techniques to probe the modifications and interactions of various cellular constituents (e.g., using one- and two-hybrid screens). (cshlpress.com)
  • data
  • In contrast, herein we analyze Hi-C data for budding yeast and identify 200-kb scale TADs, whose boundaries are enriched for transcriptional activity. (pnas.org)
  • The basis of these arguments is on the interaction data of human CXorf26 as well as its yeast homolog, YPL225W. (wikipedia.org)
  • study
  • The NCRR Yeast Resource Center at the University of Washington facilitates the study of yeast protein complexes by providing access to techniques such as mass spectrometry, two-hybrid arrays, deconvolution fluorescence microscopy and protein structure prediction. (factbites.com)
  • mate
  • This means that each yeast cell is of a certain mating type and can only mate with a cell of the other mating type. (wikipedia.org)