• B. melitensis could be identified by the amplification of fragments of BCSP31, omp2b/2a and omp31 using pair of primers B4/B5, JRF/JPR-ab and omp31. (nih.gov)
  • Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. (nih.gov)
  • Protective responses of the immunized mice were evaluated by intraperitoneal challenge with pathogenic Brucella melitensis 16M and Brucella ovis PA76250. (bvsalud.org)
  • We have developed a combinatorial polymerase chain reaction (PCR) procedure to identify four major species of the genus Brucella simultaneously. (nih.gov)
  • is considered as the most pathogenic species, followed by B. suis , whereas B. abortus is the mildest type of brucellosis. (medscape.com)
  • During the past 3 decades, contacts between cetaceans and humans have increased worldwide ( 1 ), augmenting the risk for transmission of pathogenic Brucella spp. (cdc.gov)
  • Additionally, Brucella isolates from marine mammals can cause human infections. (medscape.com)
  • 9 ). Genotyping of Brucella isolates was performed by PCR, using 5′-GGCTGATCTCGCAAAGAT-3′ and 5′-CCAGGTCCTTGGCTTCCTTGAG-3′ primers (Invitrogen Corporation, Carlsbad, CA, USA) for the amplification of ribosomal protein L12 and PCR- StyI restriction fragment length polymorphism of the omp2b locus, as described ( 11 , 12 ). (cdc.gov)
  • antibodies and molecular methods for the detection of Brucella spp. (medscape.com)
  • Ten striped dolphins, Stenella coeruleoalba, stranded along the Costa Rican Pacific coast, had meningoencephalitis and antibodies against Brucella spp. (cdc.gov)
  • from blood, tissue specimens, body fluids and bone marrow, serological tests for the detection of anti- Brucella spp. (medscape.com)
  • [ 7 ] The most commonly used methods for detection and segregation of Brucella spp. (medscape.com)
  • Molecular detection of Brucella spp. (nih.gov)
  • Fresh tissue impressions or pellets from supernatants of macerated tissues were fixed with cold 3% paraformaldehyde for 15 min on ice and subjected to immunofluorescence for detection of Brucella spp. (cdc.gov)
  • Identification of B. canis could be achieved when the amplicons of omp2b/2a were detected by B. canis-specific primers, as could the identification of BCSP31 and omp31. (nih.gov)
  • PCR using these primers gave rise to specific patterns of amplification for each Brucella spp. (nih.gov)
  • is associated with a risk of laboratory-acquired infections and time consuming, and culture sampling sensitivity is often low, depending on the culture medium, Brucella species, disease stage and quantity of circulating bacteria. (medscape.com)
  • How do bacteria localize proteins to the cell pole? (google.co.cr)
  • Indeed, Brucella marine strains are capable of infecting humans and livestock ( 3 , 4 ). (cdc.gov)
  • Polymerase chain reaction (PCR) technique offers a sensitive and specific way of detecting Brucella spp. (medscape.com)
  • B. abortus could be identified when fragments of BCSP31 and omp2b/2a were amplified by B. abortus-specific primers. (nih.gov)
  • For these tests, rabbit immunoglobulin (Ig) G anti- B. abortus lipopolysaccharide (LPS) was produced and isolated as described elsewhere ( 5 ). (cdc.gov)