AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesHealth Care
Brucella abortusBrucellaBrucellosisBrucellosis, BovineBrucella VaccineBrucella melitensisBrucella ovisBrucella canisAbortion, VeterinaryAntibodies, BacterialAgglutination TestsCattleErythritolChlamydophilaBisonAntigens, BacterialBrucella suisComplement Fixation TestsOchrobactrum anthropiBacterial VaccinesMice, Inbred BALB CBacterial ProteinsVirulenceSpleenRose BengalDNA, BacterialMacrophagesBuffaloesLipopolysaccharidesBacterial Outer Membrane ProteinsVaccinationSheep DiseasesHydroxybenzoatesGoatsGlomerulonephritisPharyngitisStreptococcal InfectionsStreptococcus pyogenesRheumatic FeverGlomerulonephritis, MembranoproliferativePenicillin V
BrucellosisSuisRB51CattleCanisInfectionBacteriaVaccine strainSpeciesOvisSerologicalOrganismsPathogenAbortionAntibodyIsolatesHumansChlamydiaIncubation periodMiceVirulentSerumBrothBisonInfectHerdsSurvivalContributesEfficacyInnateVirulenceSeropositivityGram-negativeSeroprevalenceRoughCellsMeSHInfectionsCulturesSurprisinglyEssentialVaccinationImmuneGeneAssay
Brucellosis25
  • Brucella abortus is a Gram-negative bacterium in the family Brucellaceae and is one of the causative agents of brucellosis. (wikipedia.org)
  • Brucellosis: Brucella Abortus" (PDF). (wikipedia.org)
  • None of the exposed persons reported having previously had brucellosis or being unintentionally inoculated with Brucella vaccine. (cdc.gov)
  • Editorial Note: Brucellosis, also known as 'undulant fever' or 'Bangs disease,' is a systemic infection caused by Brucella sp. (cdc.gov)
  • The IDEXX Brucellosis Serum X2 Ab Test is an enzyme immunoassay for the detection of antibodies against Brucella abortus in individual serum and plasma, and pools of up to 10 individual serum or plasma samples of ruminants. (idexx.co.uk)
  • Brucellosis in cattle is a disease caused by Brucella abortus ( B. abortus ), a facultative, intracellular bacterium. (idexx.co.uk)
  • Brucellosis is a worldwide disease of humans and livestock that is caused by a number of very closely related classical Brucella species in the alpha-2 subdivision of the Proteobacteria. (nih.gov)
  • Three types of the bacteria that cause brucellosis - Brucella abortus , Brucella melitensis and Brucella suis - are designated as select agents. (cdc.gov)
  • Using Brucella abortus Strain 19 (S19) to control bovine brucellosis is restricted due to induce antibodies to the O-side chain of the smooth lipopolysaccharide (LPS) which may be difficult to differentiate vaccinated and infected animals. (ijbiotech.com)
  • The pathology of brucellosis reflects the outcome of the battle between the host genome and the Brucella genome. (ijbiotech.com)
  • Brucellosis, caused by Brucella abortus, is a major disease of cattle and humans worldwide distributed. (nau.edu)
  • Brucella abortus is the causative organism of the disease undulating fever or Brucellosis. (sciences360.com)
  • The immunogenic B. abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. (ac.ir)
  • Brucella abortus (B. abortus) is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant cells, and the causative agent of brucellosis. (elsevierpure.com)
  • This investigation is associated with the third known case of brucellosis from Brucella RB51 due to raw milk acquired in the U.S., since August 2017. (cdc.gov)
  • When ordering blood cultures to diagnose brucellosis, please advise the laboratory that blood culture may grow Brucella and that appropriate laboratory containment and precautions should be observed. (cdc.gov)
  • Although various potential intermediate hosts have harbored brucellosis in the extra-Mediterranean world, dairy cattle infected with B abortus have been particularly important hosts in North America. (medscape.com)
  • Outbreaks of epizootic bovine abortion due to B abortus should alert health care providers to the possibility of human brucellosis. (medscape.com)
  • Detection of antibodies to Brucella abortus and Brucella suis, the causative agents of brucellosis, using buffered acidified plate antigen (BAPA). (tamu.edu)
  • Detection of antibodies to Brucella abortus and Brucella suis, the causative agents of brucellosis, by card agglutination. (tamu.edu)
  • Brucellosis is considered a major study highlighted the antimicrobial A matched case-control study was animal-related occupational health resistance of Brucella spp. (who.int)
  • Brucellosis is caused by members of the Brucella genus. (who.int)
  • Brucellosis in dogs is usually overlooked and yet there is extensive contact between humans and their pets.Objective: This study investigated brucellosis in children and dogs using a confirmatory serological testing series that screens for three Brucella sp.Methods: Residual blood samples from malaria smear-negative febrile children were collected and tested for Brucella sp and malaria parasite. (bvsalud.org)
  • Although brucellosis is mainly a bacterial disease of animals, several species of Brucella bacteria are known to cause disease in humans. (medscape.com)
  • Approximately 70%-75% of US brucellosis cases reported annually to CDC are due to the bacterial species B melitensis and B abortus . (medscape.com)
Suis4
  • small Gram-negative coccobacilli that can infect cattle (infection with B. abortus), goats and sheep (B. melitensis), pigs (B. suis), and dogs (B. canis). (cdc.gov)
  • We report the complete genome sequence of Brucella abortus field isolate 9-941 and compare it to those of Brucella suis 1330 and Brucella melitensis 16 M. The genomes of these Brucella species are strikingly similar, with nearly identical genetic content and gene organization. (nih.gov)
  • Several fragments previously identified as unique to either B. suis or B. melitensis were present in the B. abortus genome. (nih.gov)
  • Even though several fragments were shared between only B. abortus and B. suis, B. abortus shared more fragments and had fewer nucleotide polymorphisms with B. melitensis than B. suis. (nih.gov)
RB5112
  • The National Animal Disease Center, U.S. Department of Agriculture (USDA), identified the B. abortus isolate from the calf as the RB51 vaccine strain. (cdc.gov)
  • The vaccine had caused active B. abortus infection because the 14-month-old heifer delivering the calf was not known to be pregnant when she was vaccinated with RB51 at approximately 8 months of age, which was within the specified age range for vaccination. (cdc.gov)
  • Evidence that intrauterine infection was caused by the RB51 vaccine strain, and not by field strains of B. abortus or by S19, included immunohistochemical staining specific for RB51 (negative for S19), RB51-specific titer of greater than 1:10,000 on experimental dot-blot assay measuring antibody to RB51, and RB51-specific DNA sequences identified by polymerase chain reaction (PCR). (cdc.gov)
  • Characterization of recombinant B. abortus strain RB51SOD toward understanding the uncorrelated innate and adaptive immune responses induced by RB51SOD compared to its parent vaccine strain RB51. (ijbiotech.com)
  • Complementation of Brucella abortus RB51 with a functional wboA gene results in O-antigen synthesis and enhanced vaccine efficacy but no change in rough phenotype and attenuation. (ijbiotech.com)
  • Efficacy of Brucella abortus vaccine strain RB51 compared to the reference vaccine Brucella abortus strain 19 in water buffalo. (ijbiotech.com)
  • The New York State Department of Health and Pennsylvania Department of Health are investigating Brucella RB51 exposures that may be connected to consuming raw (unpasteurized) milk from Miller's Biodiversity Farm in Quarryville, Pennsylvania. (cdc.gov)
  • Milk samples from the dairy tested positive for Brucella strain RB51. (cdc.gov)
  • Brucella strain RB51 is a live-attenuated cattle vaccine strain, which can be shed in milk and can cause infections in humans. (cdc.gov)
  • Brucella abortus vaccine strain RB51 infection and exposures associated with raw milk consumption - Wise County, Texas, 2017. (cdc.gov)
  • Finally, people can become infected with Brucella RB51, a strain of B abortus that is used to vaccinate cattle in the United States and other parts of the world. (medscape.com)
  • However, B canis and Brucella RB51 won't show up on serology, so culture is needed to confirm the infection. (medscape.com)
Cattle10
  • B. abortus causes abortion and infertility in adult cattle and is a zoonosis which is present worldwide. (wikipedia.org)
  • Cattle and other livestock are the major host species for the bacteria B. abortus. (wikipedia.org)
  • B. abortus can last a lot longer in animals if they are not watched closely and if the cattle are not treated for it. (wikipedia.org)
  • A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. (harvard.edu)
  • In addition, infection may occur as the result of cattle ingesting B. abortus -contaminated feed or water. (idexx.co.uk)
  • Present study is aimed to determine the excretion of Brucella abortus organisms in milk of vaccinated lactating cattle and buffaloes. (scholarsresearchlibrary.com)
  • Eradication of B. abortus from cattle is nearly complete in the U.S., but the disease still occurs in some wild bison and elk herds in the western U.S. (cdc.gov)
  • We characterized 188 B. abortus isolates from CR recovered from cattle, humans and water buffalo, from 2003 to 2018, and whole genome sequencing (WGS) was performed in 95 of them. (nau.edu)
  • Brucella abortus causes spontaneous abortion in cattle. (sciences360.com)
  • Brucella abortus is a facultative intracellular gram-negative bacterial pathogen that causes abortion in pregnant cattle and undulant fever in humans. (ac.ir)
Canis4
Infection22
  • On May 26-27, 1997, nine persons (a farmer, four veterinary clinicians, and four veterinary students) in Manhattan, Kansas, participated in an attempted vaginal delivery, a cesarean delivery, and a necropsy on a stillborn calf that died because of Brucella abortus infection. (cdc.gov)
  • The infection was confirmed by isolation of B. abortus from placental and fetal lung tissue cultures. (cdc.gov)
  • Brucella abortus and Pregnancy in Mice: Impact of Chronic Infection on Fertility and the Role of Regulatory T Cells in Tissue Colonization. (harvard.edu)
  • B Lymphocytes provide an infection niche for intracellular bacterium Brucella abortus. (harvard.edu)
  • However, NOD1, NOD2, and Rip2 knockout mice were no more susceptible to infection with virulent B. abortus than wild-type mice. (hindawi.com)
  • Taken together, this study demonstrates that NOD1, NOD2 and Rip2 are dispensable for the control of B. abortus during in vivo infection. (hindawi.com)
  • The immune response against Brucella infection involves many molecules and cells to trigger a Th1 immune response and activation of CD8+ T cells [ 12 - 14 ]. (hindawi.com)
  • The innate immune response against B. abortus infection begins with the recognition of molecular structures related to this pathogen by receptors such as Toll-like receptors (TLRs) [ 15 ]. (hindawi.com)
  • Moreover, our group and others have demonstrated that MyD88 is essential for host control of Brucella infection in vivo and the induction of proinflammatory cytokines [ 21 ]. (hindawi.com)
  • So far, no study has demonstrated the role of NOD-like receptors in the control of Brucella infection. (hindawi.com)
  • Herein, we have shown that NOD1, NOD2, or the adaptor molecule Rip2 plays no role in enhancing resistance to B. abortus infection in vivo . (hindawi.com)
  • Exposure to most species of Brucella , such as those associated with certain types of animals, could potentially lead to infection. (cdc.gov)
  • Serological testing for antibodies to Brucella will also indicate infection. (sciences360.com)
  • We used Brucella culture of fetus/calf tissues to determine the efficacy of vaccination for preventing infection, and we found that the number of infected fetuses/calves did not differ between controls and vaccinates (P = 0.14). (lsu.edu)
  • In the present study, we found that production of regulated upon activation normal T-cell expressed and secreted (RANTES) due to B. abortus infection contributes to abortion in pregnant mice. (elsevierpure.com)
  • B. abortus infected pregnant interferon-γ (IFN-γ) knockout mice died within 15 days of infection, but non-pregnant IFN-γ knockout mice were still alive. (elsevierpure.com)
  • With infection by wild type B. abortus, a large amount of RANTES production was observed in pregnant IFN-γ knockout mice, and induction of RANTES was also observed in normal pregnant mice infected with the wild type, but not in those infected with the intracellular replication-defective mutant. (elsevierpure.com)
  • Neutralization of RANTES, induced by B. abortus infection, served to prevent abortion. (elsevierpure.com)
  • The effects of Cobalt (II) chloride (CoCl2) in the context of Brucella abortus (B. abortus) infection have not been evaluated so far. (gnu.ac.kr)
  • Secondly, the activation of trafficking regulators LAMP1, LAMP2, and lysosomal enzyme GLA at the transcriptional level activated immune responses, weakening the B. abortus growth at 4 h post-infection (pi). (gnu.ac.kr)
  • Altogether, CoCl2 treatment contributed to host resistance against B. abortus infection with immunomodulatory effects. (gnu.ac.kr)
  • [ 7 ] Surprisingly, infection with Brucella species accounts for as many as 10% of laboratory-acquired infections, 24% of laboratory-acquired bacterial infections, and 11% of occupational-exposure deaths in the United States. (medscape.com)
Bacteria1
  • The complete genomic sequence of B. abortus provides an important resource for further investigations into determinants of the pathogenicity and virulence phenotypes of these bacteria. (nih.gov)
Vaccine strain2
  • Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes. (ijbiotech.com)
  • The vaccinate groups received Brucetta abortus vaccine strain 19 (S19) by hand-delivered intramuscular injection. (lsu.edu)
Species6
  • Brucella has twelve different kinds of species, one being Brucella abortus. (wikipedia.org)
  • 5. Zygmunt MS, Blasco JM, Letesson JJ, Cloeckaert A, Moriyon I. DNA polymorphism analysis of Brucella lipopolysaccharide genes reveals marked differences in O-polysaccharide biosynthetic genes between smooth and rough Brucella species and novel species-specific markers. (ijbiotech.com)
  • This woman was infected with another Brucella species, Brucella melitensis. (sciences360.com)
  • Because the diagnostic test and treatment vary, it is important to identify whether a patient is infected with Brucella , and if so, which [species of] Brucella is causing disease. (medscape.com)
  • Each species of Brucella has distinct risk factors. (medscape.com)
  • Most clinical diagnostic laboratories are able to run serologic tests, which detect an antibody response to most of the Brucella species. (medscape.com)
Ovis1
  • Characterization of Brucella abortus O-polysaccharide and core lipopolysaccharide mutants and demonstration that a complete core is required for rough vaccines to be efficient against Brucella abortus and Brucella ovis in the mouse model. (ijbiotech.com)
Serological1
Organisms2
  • The major mode of disease transmission is ingestion of B. abortus organisms that may be present in tissues of aborted foetuses, foetal membranes and uterine fluids. (idexx.co.uk)
  • Sterile Potato infusion agar (PIA) media plate was used for detection of Brucella abortus organisms. (scholarsresearchlibrary.com)
Pathogen2
  • Here we investigated its role in the α-Proteobacterium Brucella abortus, a zoonotic intracellular pathogen. (unamur.be)
  • Enzootic abortion is caused by a pathogen called Chlamydophila abortus (Chlamydia psittaci serotype 1). (oregonstate.edu)
Abortion2
  • Location , age, parity , and abortion history were associated with Brucella spp. (bvsalud.org)
  • The questionnaire survey established that 44% of respondents had recently faced reproductive disease challenges within their flocks, with 34% correctly identifying abortion causes and only 10%, 6% and 4% having specific knowledge of Brucella spp. (bvsalud.org)
Antibody6
  • Murine monoclonal IgG1 antibody reactive with LPS of B. abortus. (arxsciences.com)
  • Karimi R, Mostafaie A, Tabaraie B, Bahrami Y, Abdolalizadeh J. Reaction of Native and Denatured Brucella abortus (S19) Proteins with Antibody Using Affinity Chromatography and Immunoblotting. (ac.ir)
  • Description: Quantitative sandwich ELISA for measuring Human Brucella Antibody IgM (Brucella-Ab-IgM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (iowaodes.com)
  • Description: Quantitative sandwich ELISA for measuring Human Brucella Antibody IgA (Brucella-Ab-IgA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (iowaodes.com)
  • Human IgG antibody Laboratories manufactures the canine brucella test saliva reagents distributed by Genprice. (postgenomeconsortium.com)
  • Description: Quantitativesandwich ELISA kit for measuring Human Brucella Antibody (IgG) in samples from serum, plasma, cell culture supernates, tissue homogenates. (postgenomeconsortium.com)
Isolates1
  • Our results show circulation of five B. abortus lineages (I to V) in CR, phylogenetically related to isolates from the United States, United Kingdom, and South America. (nau.edu)
Humans1
  • There are many different ways B. abortus can spread from the different animals and even to humans. (wikipedia.org)
Chlamydia3
  • Toxoplasma gondii and Chlamydia abortus in small ruminants from selected smallholder commercial farms of Zimbabwe. (bvsalud.org)
  • Toxoplasma gondii , and Chlamydia abortus have long been recognized as zoonoses and significant causes of reproductive failure in small ruminants globally. (bvsalud.org)
  • Toxoplasma gondii and Chlamydia abortus in 398 small ruminants from four districts of Zimbabwe (Chivi, Makoni, Zvimba, and Goromonzi) using Indirect-ELISAs. (bvsalud.org)
Incubation period2
  • Once infected with Brucella the incubation period can be from one to two months. (sciences360.com)
  • Although acquired outside the United States, these cases are not diagnosed until after the patient has returned home, due to the long incubation period for Brucella . (medscape.com)
Mice2
  • These results indicate that the production and function of RANTES are correlated with IFN-γ in pregnant mice infected with B. abortus. (elsevierpure.com)
  • Finally, our results demonstrated that CoCl2 administration induced the production of serum cytokines IFN-gamma and IL-6, which is accompanied by dampened Brucella proliferation in the spleen and liver of treated mice, and reduced the splenomegaly and hepatomegaly. (gnu.ac.kr)
Virulent1
  • Brucella abortus virulent strain S2308 was obtained from our laboratory collection [ 22 ]. (hindawi.com)
Serum2
  • In this study proteins of Brucella abortus (S19) was extracted by a mild method and reaction of the extracted proteins with serum of infected human and goat and immunized rabbit compared by affinity chromatography and immunoblotting. (ac.ir)
  • The Canine Brucella Test Saliva reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (postgenomeconsortium.com)
Broth1
  • The strain S2308 was grown in Brucella Broth liquid medium (BB) (DIFCO) at 37°C under constant agitation. (hindawi.com)
Bison1
Infect1
  • Effects of gamma radiation and azathioprine on Brucella abortus infect" by P. H. Elzer, G. E. Rowe et al. (lsu.edu)
Herds1
  • Unpasteurized soft cheeses can present a risk and milk to produce such cheese is often sourced from certified Brucella free herds. (sciences360.com)
Survival1
  • Temperature plays a huge role in the survival of B. abortus. (wikipedia.org)
Contributes2
  • In conclusion, we propose that PdeA contributes to bacterial morphology and virulence in B. abortus, but it is not crucial for polarity and asymmetric growth. (unamur.be)
  • These results suggest that znuA does not contribute to intracellular trafficking of B. abortus , but contributes to utilization of zinc required for intracellular growth. (go.jp)
Efficacy2
  • Efficacy of single calfhood vaccination of elk with Brucella abortus s" by Thomas J. Roffe, Lee C. Jones et al. (lsu.edu)
  • Based on these data, single calfhood vaccination with S19 has low efficacy, will likely have only little to moderate effect on Brucella prevalence in elk, and is unlikely to eradicate the disease in wildlife of the GYA. (lsu.edu)
Innate2
  • Brucella abortus enters phagocytes that invade human and animal innate defenses which in turn, cause chronic disease in the host. (wikipedia.org)
  • The lipopolysaccharide core of Brucella abortus acts as a shield against innate immunity recognition. (ijbiotech.com)
Virulence1
  • Brucella abortus depends on pyruvate phosphate dikinase and malic enzyme but not on Fbp and GlpX fructose-1,6-bisphosphatases for full virulence in laboratory models. (harvard.edu)
Seropositivity1
  • Location was also associated with both T. gondii and C. abortus seropositivity. (bvsalud.org)
Gram-negative1
  • Brucella is a Gram-negative bacterium which is pathogenic of human and animals [ 11 ]. (hindawi.com)
Seroprevalence3
Rough1
  • a stable rough strain of Brucella abortus . (cdc.gov)
Cells1
  • Firstly, we found that CoCl2 treatment inhibited the phagocytosis of B. abortus into RAW 264.7 cells. (gnu.ac.kr)
MeSH1
  • Brucella abortus" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
Infections2
  • Brucella infections respond to treatment with the antibiotics doxycycline combined with rifampacin. (sciences360.com)
  • In pregnant patients, Brucella infections can be associated with miscarriage. (cdc.gov)
Cultures1
Surprisingly1
  • Surprisingly, deletion of all predicted cyclic-di-GMP synthesizing or degrading enzymes did not drastically impair the growth of B. abortus, nor its ability to grow inside cell lines. (unamur.be)
Essential1
  • We found that the phosphodiesterase PdeA, the ortholog of the essential polar growth factor RgsP of the Rhizobiale Sinorhizobium meliloti, is required for rod shape integrity but is not essential for B. abortus growth. (unamur.be)
Vaccination1
  • Assurer une vaccination animale, éduquer la population en ce qui concerne les bonnes méthodes de pasteurisation du lait et de manipulation des produits carnés, et relever les niveaux de suivi de la sécurité sanitaire des aliments sont des éléments clés de la lutte contre la brucellose. (who.int)
Immune1
  • Brucella spp noncanonical LPS: structure, biosynthesis, and interaction with host immune system. (ijbiotech.com)
Gene2
  • The aim of this study was to employ gene knockout B. abortus S19 for the first time to eliminate diagnostic defects and obtain the attenuated mutant strain. (ijbiotech.com)
  • The wbkA gene, which is one of the LPS O-chain coding genes, was knocked out in vaccinal Brucella abortus S19. (ijbiotech.com)
Assay1
  • In the second phase, Brucella toethanol (2-MET) in the assay disulfide spp. (who.int)