• 3). A produção de antígenos e peptídeos sintéticos possibilitou o desenvolvimento de testes que permitem a detecção de anticorpos contra o VHC (anti-VHC), como os testes ELISA ( enzyme-linked immunosorbent assay ) e RIBA ( recombinant immunoblot assay ). (scielosp.org)
  • With Affymetrix' ViewRNA ISH portfolio for tissue samples, researchers can rapidly design assays for virtually any mRNA transcript or long noncoding RNA in any tissue type. (technologynetworks.com)
  • ORB provides cost-effective customizable QuantiGene assays to rapidly characterize mRNA and microRNA targets as standalone projects or confirmation studies following broader interrogation strategies such as RNA sequencing and gene expression and microRNA microarray . (oceanridgebio.com)
  • For example, researchers have designed probes for the bDNA assay to measure cellular mRNA levels. (mybiosource.com)
  • and (iii) in what are often referred to as ''gene expression'' assays, QRT-PCR can be used to determine relative mRNA expression levels for different disease states. (asmscience.org)
  • We have evaluated the efficacy of this system using synthetic standard HBV DNA and several clinical samples and have assessed the correlation between the results obtained by this assay and those obtained using the bDNA assay. (asm.org)
  • The multiplex quantitation capability of an assay is dependent upon instrument model, and ranges from 50 analytes with use of the MAGPIX, to 500 analytes with the FLEXMAP 3D. (thermofisher.com)
  • Although these probe tests are still widely used, amplification-based methods are increasingly employed for diagnosis, identification and quantitation of pathogens, and characterization of antimicrobial-drug resistance genes. (cdc.gov)
  • To detect changes in DNA, there are four broad categories of tests, which are based on hybridisation, sizing, quantitation and determination of DNA sequence. (health.gov.au)
  • In this project, surface acoustic wave (SAW) signals are generated by interdigitated transducer (IDT) on LiNbO3 and propagated into disposable silicon superstrates on which a droplet of Loop-mediated isothermal amplification (LAMP) reaction mixture has been placed. (gla.ac.uk)
  • We attempted to develop a quantitative assay system for HBV DNA that is more sensitive, accurate, and reproducible than existing systems. (asm.org)
  • Enhanced chemiluminescence immunoassay (ECi) a quantitative assay based on chemiluminescent reaction, which is as good as ELISA. (oncquest.net)
  • The RNAscope ® Assays run on the automated Ventana Discovery Ultra staining platform. (bioneer.dk)
  • The combined use of digital whole slide imaging and image analysis provides opportunities to quantify chromogenic ISH signals from both LNA-based assays and RNAscope-based assays. (bioneer.dk)
  • Despite the fact that the starting material is not preamplified, bDNA assays can detect less than 100 copies of HIV-RNA per mL of blood. (primidi.com)
  • Discriminating 93 serotypes takes a lot of beads Ger Rijkers and the Pneumococcal Consortium are working to develop standardized multiplex assays for S. pneumoniae antibodies. (luminexcorp.com)
  • The indomitable aspect of HIV-1 infection is not that HIV-1 proviral DNA is integrated into host DNA but that it can also turn itself off, remaining invisible to drug or immune surveillance. (asm.org)
  • Important factors that must be considered when selecting HIV-1 diagnostic assays for pediatric patients and when choosing the timing of such assays include the age of the child, potential timing of infection of the child, whether the infection status of the child's mother is known or unknown, the antiretroviral exposure history of the mother and of the child, and characteristics of the virus. (aappublications.org)
  • A diagnosis of HIV-1 infection can be made on the basis of 2 positive HIV-1 DNA or RNA assay results. (aappublications.org)
  • Many clinicians confirm the absence of HIV-1 infection with a negative HIV-1 antibody assay result at 12 to 18 months of age. (aappublications.org)
  • However, the assay may be negative early in acute HCV infection, in immunosuppressed individuals. (oncquest.net)
  • Anti-HCV antibodies can be detected by rapid immunoassays, enzyme-linked immunosorbent assays (ELISA), or chemiluminescent immunoassays. (oncquest.net)
  • 14-gene assay interrogates circulating tumor cells Liquid biopsies are opening new avenues for cancer research and diagnosis, but even experts in the field continue to nail down the best protocols for analyzing the cell-free DNA and circulating tumor cells (CTCs) contained in these peripheral blood samples. (luminexcorp.com)
  • A set of six primers were designed derived from the dsbE-like gene of A.pleuropneumoniae and validate the assay using 9 A. pleuropneumoniae reference/field strains, 132 clinical isolates and 9 other pathogens. (bvsalud.org)
  • Following two months of treatment, the rats were euthanized and the gene expressions of BMP-2, OPG, RANKL and MCSF in the femoral bones were measured using a branch - DNA technique. (biomedcentral.com)
  • The DNA sequence derived revealed an operon-like gene structure with two open reading frames encoding an 11,122- and a 57,956-Da protein. (faintpower.ml)
  • The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. (bvsalud.org)
  • andc) detecting the amplified product or amplified signal of the translocated gene RNA or cDNA, or the fusion gene RNA or cDNA, or the translocated gene RNA or cDNA and the fusion gene RNA or cDNA. (patentsencyclopedia.com)
  • Although mainly a research tool, nucleic acid sequence analysis coupled with target amplification is clinically useful and helps detect and identify previously uncultivatable organisms and characterize antimicrobial resistance gene mutations, thus aiding both diagnosis and treatment of infectious diseases ( 5 , 8 , 9 ). (cdc.gov)
  • Using the LightCycler ® 480 System Bioneer offers qPCR based gene expression analyses in 96- or 384-well plate formats employing either TaqMan (5'-hydrolysis) probe or SYBR-green based assays. (bioneer.dk)
  • In zebrafish (F)ISH non-enzymatic and enzymatic amplification systems have been employed to obtain enhanced signal intensities and signal-to-noise ratios. (su.se)