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  • infertility
  • It is often overlooked that human ESCs are generated from in vitro cultured, often surplus/'discard', embryos considered unsuitable for transfer in infertility clinics. (omicsdi.org)
  • viability
  • More recently this technique has been applied to the microinjection of CRISPR/Cas9 reagents into two-cell stage mouse embryos.7 The authors demonstrated significant increase in the knock-in efficiency and high viability of embryos using their method. (wpiinc.com)
  • In contrast to conventional microinjection, in targeted microinjection using the MICRO-ePORE™, the membrane does not tear and thus allows for superior viability of embryos. (wpiinc.com)
  • MICRO-ePORE™ was designed for a range of applications including generation of CRISPR/Cas9 mediated knock-in mice with large insertions by microinjection into two-cell stage embryos with high viability.7 The MICRO-ePORE™ has delivered accurate microinjection of morpholino oligomers (anti-sense "knockdown") in zebrafish tails. (wpiinc.com)
  • Therefore, several areas are investigated in search of additional markers of viability in order to reduce multiple gestation rates and improve implantation rates, but all have yet to prove their value in a clinical setting. (clinicaltrials.gov)
  • The present study investigated whether respiration rates of individual embryos were correlated with gestation length, type of parturition, birth weight, sex, and viability of the offspring. (forskningsdatabasen.dk)
  • Mitochondrial DNA
  • May-Panloup P, Chretien MF, Malthiery Y, Reynier P. Mitochondrial DNA in the oocyte and the developing embryo. (springermedizin.de)
  • Anderson S, de Bruijn MHL, Coulson AR, Eperon IC, Sanger F, Young IG (1982) Complete sequence of bovine mitochondrial DNA conserved features of the mammalian mitochondrial genome. (springer.com)
  • different stages
  • In this study, the levels of AEA and two other N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. (jove.com)
  • Genome-wide expression profiling by microarrays has proved a highly effective tool for high throughput analysis of transcriptomes of tissues, cell lines, or any biological mRNA pools, usually across different stages, conditions, or treatments. (biomedcentral.com)
  • Assay
  • Zavos, P.M., and Cohen, M.R. Bovine mucus penetration test: An Assay for normospermic and oligoteratoasthenospermic (OTA syndrome) patients. (zavos.org)
  • Accuracy of a rapid Y-Chromosome-Detecting Bovine Embryo Sexing Assay", personal communication. (patentgenius.com)
  • genes
  • We hypothesise that the quality of the embryo is reflected by the transcription of selected genes, the cleavage kinetics, and the metabolic profile. (clinicaltrials.gov)
  • expression
  • Heat stress increases embryo loss and reduces estrous expression, embryo quality and size, endocrine hormones, and immune function. (extension.org)
  • The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. (jove.com)
  • Notably, there are beneficial effects of mtDNA supplementation over the use of nuclear reprograming agents in terms of regulating gene expression in embryos. (springer.com)
  • Each stage contained a defined miRNA population, some of which showed stable expression while others showed progressive changes between stages that were subsequently confirmed by quantitative reverse transcription polymerase chain reaction (RT-PCR). (edu.au)
  • Pig zygotic genome activation was confirmed to occur at the 4-cell stage via genome-wide gene expression analysis. (biomedcentral.com)
  • endometrium
  • The endometrium responds differently to cloned versus fertilized embryos. (publish.csiro.au)
  • The receptors of both TH and TSH increase in the receptive endometrium, suggesting that they are important for implantation, possible by influencing inflammatory mediators such as leukemia inhibitory factor. (frontiersin.org)
  • follicular
  • We report on the effects of n-3 versus n-6 PUFA-enriched diets fed to 36 ewes over a 6-week period, prior to ovarian stimulation and follicular aspiration, on ovarian steroidogenic parameters and embryo quality. (bioscientifica.com)
  • characterize
  • The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. (jove.com)
  • transcripts
  • Transcripts for HDL and low-density lipoprotein (LDL) receptors ( SCARB1 and LDLR ) and stearoyl-CoA desaturase ( SCD ) are reported in sheep embryos. (bioscientifica.com)
  • Nonetheless, we identified 67 transcripts that significantly differed between these two groups of embryos at a 15% false discovery rate, including 33 transcripts showing at least a two-fold difference. (biomedcentral.com)
  • reproductive
  • DNA methylation reprogramming during oogenesis and interference by reproductive technologies: studies in mouse and bovine models. (publish.csiro.au)
  • RFD is the official journal of the International Embryo Technology Society and the Society for Reproductive Biology . (publish.csiro.au)
  • 2016). The extensive deleterious effects of cadmium on the reproductive system and developing embryos have been described, especially in the male (de Angelis et al. (deepdyve.com)