• Composite from Widefield fluorescence microscopy showing an image of bovine pulmonary artery endothelial (BPAE) cells acquired using the FS5 Spectrofluorometer with microscope add-on. (edinst.com)
  • Widefield fluorescence microscopy enables detailed cellular images like that of bovine pulmonary artery endothelial (BPAE) cells shown in Figure 1. (edinst.com)
  • The sample investigated was FluoCells™ Prepared Slide #1 from Invitrogen™ which contains bovine pulmonary artery endothelial (BPAE) cells. (edinst.com)
  • In order to determine whether PJ is capable of increasing the production of NO by vascular endothelial cells, PJ was tested for its capacity to upregulate and/or activate endothelial NO synthase (eNOS) in bovine pulmonary artery endothelial cells. (nih.gov)
  • Hemodynamically, PAH is defined by a mean pulmonary artery pressure (mPAP) of ≥20 mm Hg, a pulmonary arterial wedge pressure (PAWP) of ≥15 mm Hg, and a pulmonary vascular resistance (PVR) of ≥three Wood Units (WU), as established at the sixth World Symposium on Pulmonary hypertension (PH) in Nice 2018 [ 1 ] . (encyclopedia.pub)
  • In PAH, pulmonary vascular remodeling is characterized by an accumulation of vascular cells in the pulmonary arterial wall, such as changes in the pulmonary artery smooth muscle cells (PASMCs), endothelial cells, fibroblasts, myofibroblasts, and pericytes. (encyclopedia.pub)
  • EGM™-2 BulletKit™ - Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. (lonzabioscience.com.au)
  • EGM™-2 MV BulletKit™ - Coronary and Iliac artery, human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. (lonzabioscience.com.au)
  • Bovine pulmonary artery (BPAE) and microvascular endothelial (BMVE) cells were isolated, grown in tissue culture, and characterized by the presence of Factor VIII related antigen and LDL uptake. (johnshopkins.edu)
  • BMVE cells produced one tenth the amount of ET-1 as produced by the pulmonary artery endothelial cells under the same conditions. (johnshopkins.edu)
  • Though it has been proven within a rat style of pulmonary hypertension that there surely is a build up of endogenous NOS inhibitors, along with an increase of arginase activity in the pulmonary artery endothelial cells (11), the immediate ramifications of these endogenous NOS inhibitors on arginase possess however to been reported. (phytid.org)
  • Monolayers of bovine pulmonary artery ECs (BPAECs) in culture, following labeling of membrane phospholipids with [32P]orthophosphate, were exposed to mercuric chloride (inorganic form), methylmercury chloride (environmental form), and thimerosal (pharmaceutical form), and the formation of phosphatidylbutanol as an index of PLD activity was determined by thin-layer chromatography and liquid scintillation counting. (drcalapai.com)
  • However, these previous studies did not determine the relative contributions of COX (COX‐1 and COX‐2) and nitric oxide synthase (NOS) isoforms (endothelial constitutive (ecNOS) and inducible NOS (iNOS)) to the abnormal acinar microvessel response to HA 4 , 7 . (ersjournals.com)
  • The expression of caspase‑3, Bax, Bcl‑2, phosphorylated (p)‑p38MAPK/total (t‑)p38MAPK and p‑endothelial e nitric oxide synthase (eNOS)/t‑eNOS proteins were determined by western blotting. (spandidos-publications.com)
  • Here we show that the release of ATP induced by hypotonic stress (HTS) in bovine aortic endothelial cells (BAECs) occurs through volume-regulated anion channels (VRAC). (silverchair.com)
  • TGRL Lipolysis Products Induce Stress Protein ATF3 via the TGF-β Receptor Pathway in Human Aortic Endothelial Cells. (ucdavis.edu)
  • Shklover J, McMasters J, Alfonso-Garcia A, Higuita ML, Panitch A, Marcu L, Griffiths L . Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function. (mayo.edu)
  • Convincing data have been presented to show that production of reactive oxygen species (ROS) occurs in nonphagocytic cells, including endothelial cells ( 8 ), vascular smooth muscle cells ( 9 ), and aortic adventitial fibroblasts ( 10 ), and superoxide anion has a variety of biological functions that accompany induction of gene expression ( 11 ), cellular proliferation ( 12 ), apoptosis ( 13 , 14 ), and hypertrophy ( 15 ). (diabetesjournals.org)
  • Endothelin-1 (ET-1), a peptide product of endothelial cells, is mitogenic for fibroblasts and smooth muscle cells. (johnshopkins.edu)
  • Studies have shown that periodontitis is associated with altered expression of inflammatory mediators in gingival fibroblasts and periodontal membrane cells ( 3 , 4 ). (spandidos-publications.com)
  • Can the vascular endothelial growth factor (VEGF) and total estradiol 17β be used as a marker of equine oocyte maturation? (cloud-clone.com)
  • Resveratrol also down-regulated the increased expressions of vascular endothelial growth factor (VEGF) and kinase insert domain receptor (KDR, or VEGF receptor-2) induced by high glucose. (springer.com)
  • On the other side, vascular endothelial growth factor (VEGF), one of the strongest hyperpermeability inducers (Senger et al. (springer.com)
  • PJ and other antioxidant-containing products were found to augment the anti-proliferative action of NO (DETA/NO) on vascular smooth muscle cell (rat aorta) proliferation. (nih.gov)
  • The post-ischemic cerebral angiogenic response was inhibited by antibodies against TNFR1 but not TNFR2, and this correlated with reduced endothelial proliferation and decreased α5β1 and αVβ3 integrin expression after 4 and 7 days post-ischemia. (biomedcentral.com)
  • Consistent with these findings, in vitro studies showed that TNF-α induced endothelial proliferation and upregulation of α5β1 and αVβ3 integrins was abrogated by anti-TNFR1 but not anti-TNFR2 antibodies in cultured HBMECs. (biomedcentral.com)
  • Interestingly, brain endothelial cell (BEC) proliferation followed the same time-course, suggesting a mechanistic relationship, with the Fn-α5β1/αVβ3 integrin axis driving BEC proliferation and angiogenesis. (biomedcentral.com)
  • Primary cultured HTFs were incubated with transforming growth factor β1 (TGF-β1) alone or combined with nintedanib, and cell proliferation and migration were measured by cell counting kit-8 (CCK8) and the scratch wound assay, respectively. (molvis.org)
  • is the parent ROS molecule produced by the one electron reduction of oxygen catabolized by various enzymes including NADPH oxidase, cyclooxygenase, lipoxygenases, cytochrome P450 enzymes, enzymes in the mitochondrial electron transport chain ( Yang and Lüscher, 2002 ), and also endothelial NO ⋅ Synthase (eNOS, see below). (frontiersin.org)
  • The dominant expression of inwardly rectifying channels in cells from healthy donors is thought to support important glial cell functions such as the spatial buffering of extracellular K + . The downregulation of these channels and the less negative mean membrane potential in cells from patients should impair spatial buffering currents and neurotransmitter clearance. (arvojournals.org)
  • One of the main functions of Müller cells is the spatial buffering of the extracellular K + concentration in response to rises of neuronal activity. (arvojournals.org)
  • An increased dependence on extracellular glucose and a rightward shift in the K m for extracellular glucose were observed in NQO1-stimulated sulf-treated vs control cells. (marquette.edu)
  • Basement Membrane of Tissue Engineered Extracellular Matrix Scaffolds Modulates Rapid Human Endothelial Cell Recellularization and Promote Quiescent Behavior After Monolayer Formation. (mayo.edu)
  • Tissue engineered bovine saphenous vein extracellular matrix scaffolds produced via antigen removal achieve high in vivo patency rates. (mayo.edu)
  • Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold. (mayo.edu)
  • Adhesion of bovine airway smooth muscle cells activates extracellular signal-regulated kinases. (uchicago.edu)
  • Pulmonary myofibroblasts were divided into MFB group and MFB + hUC-MSCs group, and different doses of PFD were added to each group, and the levels of RGS2 and fibrosis markers were detected in each group. (researchsquare.com)
  • Here, the authors tested the hypothesis that mercury modulates the activity of the endothelial lipid signaling enzyme, phospholipase D (PLD), which is an important player in the endothelial cell (EC) barrier functions. (drcalapai.com)
  • Mechanisms of pathogenesis of atherosclerosis are complex interplay between bloodstream cells and arterial wall components that leads to a chronic state of vascular oxidative stress and inflammation ( Hansson and Hermansson, 2011 ). (frontiersin.org)
  • The leukotoxin produced by the bacteria initiates a cascade of inflammatory events that results in the accumulation of pleura (chest) fluid, fibrinous pleuritis (inflammation of the lung lining), and pulmonary necrosis (lung death). (thebeefsite.com)
  • Inflammation and cell-cell interactions in airway hyperresponsiveness. (uchicago.edu)
  • Fetal haemoglobin (HbF) induction is the primary treatment response, along with improved anaemia, reduced haemolysis, myelosuppression and decreased endothelial inflammation. (cdc.gov)
  • In the cardiovascular system, mercury induces hypertension in humans and animals that has wide-ranging consequences, including alterations in endothelial function. (hindawi.com)
  • Sánchez-Muñoz, F. Pulmonary Arterial Hypertension: Therapeutic Pathways. (encyclopedia.pub)
  • Pulmonary Arterial Hypertension: Therapeutic Pathways" Encyclopedia , https://encyclopedia.pub/entry/1383 (accessed November 28, 2023). (encyclopedia.pub)
  • Pulmonary arterial hypertension (PAH) is a severe disease characterized by the loss and obstructive remodeling of the pulmonary arterial wall, causing a rise in pulmonary arterial pressure and pulmonary vascular resistance, which is responsible for right heart failure, functional decline, and death. (encyclopedia.pub)
  • Pulmonary arterial hypertension (PAH) is a life-threatening disease characterized by the obstruction, loss, and remodeling of the pulmonary arteries, which increases pulmonary arterial pressure (PAP) and pulmonary vascular resistance (PVR). (encyclopedia.pub)
  • in pulmonary hypertension (9). (phytid.org)
  • The endothelial impairment connected with pulmonary hypertension continues to be from the dysfunction from the NO pathway and endogenous NOS inhibition may represent a system for the linked pulmonary vascular 138112-76-2 supplier redecorating (10). (phytid.org)
  • Moreover, PJ did not enhance promoter activity in the eNOS gene (COS-7 cells transfected with eNOS). (nih.gov)
  • Glucose-6-phosphate dehydrogenase (G-6-PDH) inhibition partially blocked NQO1 activity in control and sulf-treated cells, but G-6-PDH overexpression via transient transfection with the human cDNA alleviated neither the restriction on intact sulf-treated cell NQO1 activity nor the impact on the NADPH/NADP + ratios. (marquette.edu)
  • The antiarrhythmic effect of Amiodarone is due to at least two major properties: a prolongation of the myocardial cell action potential duration and refractory period (potassium channel inhibition) and noncompetitive α- and β-adrenergic inhibition. (toxbank.net)
  • Considering that the bioavailability of L-arginine to arginase could be elevated secondary towards the competitive inhibition of NOS by ADMA, we 138112-76-2 supplier also hypothesized that ADMA would bring about greater practical cell numbers due to elevated L-arginine fat burning capacity by arginase. (phytid.org)
  • Observations were made with and without inhibition of constitutive (endothelial constitutive NOS (ecNOS) and COX‐1) and inducible isoforms (iNOS and COX‐2) of NOS and COX. (ersjournals.com)
  • The data suggest that glucose transport in the sulf-treated cells may be insufficient to support the increased metabolic demand for pentose phosphate pathway-generated NADPH as an explanation for the NQO1 threshold. (marquette.edu)
  • The death receptor pathway plays an important role in cell apoptosis ( 12 ). (spandidos-publications.com)
  • Asymptomatic "foamy cell" phospholipidosis is common, but acute and chronic life-threatening epithelial cell apoptosis with fibrosis and inflammatory responses are observed with significant frequency. (toxbank.net)
  • This process can also involve the loss of [ 4 ] precapillary arteries and perivascular infiltration of inflammatory cells (macrophages, dendritic cells, mast cells, and B and T-lymphocytes) [ 4 ] . (encyclopedia.pub)
  • Evidence has been provided that activation of the vascular endothelial cells in the presence of the risk factors promotes oxidative stress and vascular inflammatory responses, leading to acceleration of atherosclerotic vascular disease. (frontiersin.org)
  • Sparstolonin B was showed to alleviate joint swelling in RA rats, inhibited inflammatory cell infiltration and the release of ROS, reduced damage caused by oxidative stress, and suppressed the rate of apoptosis in synovial cells. (hindawi.com)
  • Pyroptosis also releases the cell contents including IL-1 β , IL-18, and other inflammatory cytokines, causing the body to produce an inflammatory response. (hindawi.com)
  • Our findings suggest that fine PM enhances allergic inflammatory response in pulmonary tissue through mechanisms involving increased oxidative stress. (cdc.gov)
  • There are two hallmark phenotypes of allergic asthma: (1) inflammatory, due to a T helper cell type 2 (Th2)-mediated immune response resulting in elevated inflammatory cells in the airways, and (2) airway hyper-reactivity, during which volume and flow of inhaled air decreases as a consequence of airway constriction. (cdc.gov)
  • PJ was much more effective than the other products tested and elicited no effects when tested alone in the absence of added NO. Similarly, neither PJ nor the other products enhanced the anti-proliferative action of alpha-difluoromethylornithine, a stable substance that inhibits cell growth by NO-independent mechanisms. (nih.gov)
  • Mechanical stress induces auto/paracrine ATP release from various cell types, but the mechanisms underlying this release are not well understood. (silverchair.com)
  • One of the most important mechanisms and the sequence of events by which free radicals interfere with the cellular functions seem to be the lipid peroxidation leading eventually the cell death. (ncats.io)
  • In this study, we aimed to examine the effects of RCE in endothelial cells challenged with hypoxic exposure and to elucidate the underlying mechanisms. (mdpi.com)
  • To elucidate the biological mechanisms underlying pulmonary metastasis and identify superior therapeutic strategies, we developed a novel and clinically relevant murine RCC model exhibiting enhanced pulmonary metastasis. (biologists.com)
  • Ephrin family members receptor tyrosine kinases are mediators of angiogenesis that may also regulate endothelial barrier function in the lung. (biospraysehatalami.com)
  • This is consistent with our previous work in the chronic hypoxia model in which transgenic mice lacking endothelial expression of α5 integrin (α5-EC-KO mice) showed a delayed and reduced angiogenic response to mild hypoxia [ 6 ]. (biomedcentral.com)
  • The concept that disease rooted principally in chronic aberrant constitutive and reactive activation of mast cells (MCs), without the gross MC neoplasia in mastocytosis, first emerged in the 1980s, but only in the last decade has recognition of "mast cell activation syndrome" (MCAS) grown significantly. (degruyter.com)
  • Future treatment to lessen exacerbations of chronic obstructive pulmonary disease. (uchicago.edu)
  • Triglyceride-rich lipoprotein lipolysis products increase blood-brain barrier transfer coefficient and induce astrocyte lipid droplets and cell stress. (ucdavis.edu)
  • The administration of Sparstolonin B inhibited the secretion of IL-17 from Th17 cells and triggered the secretion of TGF- β from Treg cells, thus leading to the reduced expression of TLR4, MyD88, and NF- κ B, and the suppression of TNF- α secretion. (hindawi.com)
  • Augmentation of eosinophil degranulation and LTC(4) secretion by integrin-mediated endothelial cell adhesion. (uchicago.edu)
  • BPAE cells contain angiotensin converting enzyme (ACE), which is a vital component in the regulation of blood pressure by constricting and dilating blood vessels. (edinst.com)
  • In general, pulmonary endothelial dysfunction contributes to pulmonary vascular remodeling in all groups of PAH [ 5 ] [ 6 ] . (encyclopedia.pub)
  • Pyroptosis is a newly discovered form of cell death that differs from apoptosis and necrosis [ 6 ]. (hindawi.com)
  • According to Smith, Clinically, the BTV seems to present as underlying endothelial cell damage resulting in a vasculitis causing edema and eventually necrosis of epithelial and mucosal surfaces. (purdue.edu)
  • Primary pulmonary neoplasia in cats: assessment of computed tomography findings and survival. (ucdavis.edu)
  • Its effect attenuating bleomycin-induced pulmonary fibrosis in mice is related with the increase of RGS2. (researchsquare.com)
  • Mice underwent intrarenal implantation using luciferase-expressing Renca, a murine renal adenocarcinoma cell line. (biologists.com)
  • Among other effects, this combining activity protects pancreatic islets against damage by cytokines and prevents myocardial and pulmonary perfusion injuries. (lookformedical.com)
  • Northern blot analysis showed decreased ET-1 mRNA in cells exposed to hypoxia for 48 h. (johnshopkins.edu)
  • To determine which EphA receptors are portrayed in the standard rat lung we performed real-time RT-PCR evaluation of EphA receptor mRNA appearance for all those EphA receptors previously been shown to be portrayed in regular bovine Eribulin Mesylate lung tissues (19). (biospraysehatalami.com)
  • The results of CCK‑8 and flow cytometric analyses showed that pretreatment of HUVECs with NAC or p38MAPK inhibitor (SB203580) attenuated LPS‑induced decrease in cell viability and increase in cell apoptosis. (spandidos-publications.com)
  • Bovine pericardium has been used to fabricate conduits for vascular reconstruction. (medscape.com)
  • Li C, Shklover J, Parvizi M, Sherlock BE, Alfonso Garcia A, Haudenschild AK, Griffiths LG , Marcu L. Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging. (mayo.edu)
  • Photobleaching and low penetration depth, caused by the high scattering of the visible light, limit the utility of confocal microscopy in certain applications, particularly with living cells and/or thicker tissue samples. (photonics.com)
  • Li C, Alfonso-Garcia A, McMasters J, Bec J, Weyers B, Uyesaka L, Griffiths L , Panitch A, Marcu L. Simultaneous intraluminal imaging of tissue autofluorescence and eGFP-labeled cells in engineered vascular grafts inside a bioreactor. (mayo.edu)
  • A series of caspases proteins have been shown to be activated by the death induced signal complex [a form of death ligand that binds to the corresponding death receptor on the cell surface ( 13 )], which in turn induces apoptosis. (spandidos-publications.com)
  • Membrane transport proteins that actively co-transport ASCORBIC ACID and sodium ions across the CELL MEMBRANE. (lookformedical.com)
  • The results underscore the importance of PLD signaling in mercury-induced endothelial dysfunctions ultimately leading to cardiovascular diseases. (drcalapai.com)
  • To determine whether these reductions in albumin extravasation correlated with histological evidence of pulmonary edema lungs from HV animals receiving each method of EphA2 antagonism were fixed using a microwave fixation technique to better preserve edema fluid (5 28 As demonstrated in Fig. 5 lungs from HV animals showed patchy areas of alveolar flooding and septal thickening which were not found in control animals. (biospraysehatalami.com)
  • TNFR1 is expressed on all cell types, and this is a major signaling receptor for TNF-α, whereas TNFR2 is expressed primarily by endothelial cells and hematopoietic and some neuronal populations and mediates limited biological responses [ 12 ]. (biomedcentral.com)
  • TNF-α interacts with two types of cell surface receptors, TNF receptor 1 (TNFR1) and TNFR2 [ 12 ]. (biomedcentral.com)
  • Cells from patients displayed a less negative mean membrane potential (−52.8 mV) than cells from healthy donors (−80.6 mV). (arvojournals.org)
  • However, the membrane potentials in cells from patients scattered largely between− 6 and −99 mV. (arvojournals.org)
  • The increased activity of BK channels may support the proliferative activity of gliotic cells via feedback regulation of Ca 2+ entry and membrane potential. (arvojournals.org)
  • 1 The removal of excess K + from areas of high neuronal activity must be mainly mediated by inwardly rectifying K + (K IR ) channels because these channels are the only K + channels with a high open-state probability at the hyperpolarized resting membrane potential characteristic for Müller cells. (arvojournals.org)
  • Abrin works by binding to and penetrating the cells of the body, inhibiting cell protein synthesis after being transported to the endoplasmic reticulum (ER). (wikipedia.org)
  • Applications also include cell sorting, multiphoton imaging, matrix-assisted laser desorption ionization time-of-flight (Maldi-Tof) for protein analysis, fluorescence analysis in proteomics and genomics, and a novel treatment for ischemic strokes. (photonics.com)
  • 2 3 Therefore, a high expression level of K IR channels is one of the main indicators of the differentiated state of Müller cells. (arvojournals.org)
  • The effects were also observed at very low peptide concentrations (10 -13 M) with no apparent linear correlation between peptide dosage and increase of fluorescence intensity,which implied co-expression of different corticotropin releasing factor receptor forms in the same cell. (biologists.com)
  • To determine differences of K + channel activity between Müller glial cells obtained from retinas of healthy human donors and of patients with retinal detachment and proliferative vitreoretinopathy. (arvojournals.org)
  • Using confocal laser scanning microscopy we investigated the Ca 2+ distribution in single corticotropin releasing factor- and urocortin-stimulated human skin cells. (biologists.com)
  • Moreover, at 0.1-10 μM it also exhibited neuroprotective activity against tau toxicity induced by okadoic acid in human neuroblastoma SH-SY5Y cells, that was comparable to that of estradiol and PD38. (ibecbarcelona.eu)
  • In this study we examined the effect of hypoxia on ET-1 production by bovine pulmonary vascular endothelial cells. (johnshopkins.edu)
  • In both cell types, hypoxia (0% O 2 ) significantly reduced the amount of ET-1 at 48 h. (johnshopkins.edu)
  • These data demonstrate that pulmonary vascular endothelial cells respond to hypoxia by reversibly decreasing ET-1 production, and this attenuation is likely regulated at the level of transcription. (johnshopkins.edu)
  • The incidence of renal cell carcinoma (RCC) is high, and its outcomes remain poor. (biologists.com)
  • The isolated lung was fixed on its dorsal surface on a microscope stage and perfused with Krebs-Henseleit solution containing 3% bovine serum albumin at a constant recirculating flow rate of 0.2 mL·s −1 . (ersjournals.com)
  • To identify the anatomic compartment within the lung in which EphA2 manifestation improved in the HV animals we performed immunohistochemical studies of lung cells from control and HV animals. (biospraysehatalami.com)
  • Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. (lonzabioscience.com.au)
  • The results described in this paper indicate that mercury exposure, even at low doses, affects endothelial and cardiovascular function. (hindawi.com)
  • Sickle cell anemia, which primarily affects Black or African American people, is associated with a shorter life span and life-threatening complications that can affect all parts of the body. (cdc.gov)
  • Isolation and culture were crucial, as all emerging bacteria have been grown on artificial media or cell lines or at least propagated in animals. (cdc.gov)
  • Müller cells were enzymatically isolated from retinas of healthy donors and from excised retinal pieces of patients. (arvojournals.org)
  • The inwardly rectifying K + permeability in cells from patients was strongly reduced (0.3 pA/pF) when compared with cells from healthy donors (6.0 pA/pF). (arvojournals.org)
  • and the limiting toxicity in humans is normally pulmonary, with adverse events observed in 5% of patients. (toxbank.net)
  • Immunoproteomic Identification of Noncarbohydrate Antigens Eliciting Graft-Specific Adaptive Immune Responses in Patients with Bovine Pericardial Bioprosthetic Heart Valves. (mayo.edu)
  • Bovine Respiratory Disease Complex (BRDC) continues to be the most significant and challenging feedlot health problem throughout North America. (thebeefsite.com)
  • Managing sickle cell anemia in children is complex. (cdc.gov)
  • Once in the bloodstream, mercury undergoes catalase and peroxidase-mediated oxidation in red blood cells and tissues and is transformed into inorganic mercuric mercury (Hg ++ ) and mercurous mercury (Hg + ), a process that limits its absorption [ 9 , 12 ]. (hindawi.com)