Bovine articular chondrocytes chondrocytes. Medical search. Frequent questions

Lidocaine exhibits dose- and time-dependent cytotoxic effects on bovine articular chondrocytes in vitro. (nih.gov)
PRP was tested in vivo , after bovine intrauterine administration, and in vitro on endometrial cells. (biomedcentral.com)
For AC tissue cryopreservation, intact cored cartilage discs (5 mm diameter) and diced cartilage (0.2-1 mm cubes) from the same sized discs were step cooled and stored in liquid nitrogen for up to 48 h before chondrocyte isolation and in vitro assay of cell viability and proliferative potential. (ox.ac.uk)
Transduced bovine articular chondrocytes affect the metabolism of cocultured nucleus pulposus cells in vitro: implications for chondrocyte transplantation into the intervertebral disc. (uchicago.edu)
Effects of an articular cartilage lubrication with a viscosupplement in vitro and in vivo following osteochondral fractures in horses. (ucsd.edu)
We have studied an in vitro engineered cartilage model, consisting of bovine articular chondrocytes seeded on micro-porous scaffolds and perfused with very low regimens of interstitial flow. (iusspavia.it)
Parkkinen JJ, Lammi MJ, Helminen HJ, Tammi M. Local stimulation of proteoglycan synthesis in articular cartilage explants by dynamic compression in vitro. (lievers.net)
Effects of static and cyclic compressive loading on articular cartilage plugs in vitro. (lievers.net)
Currently, the only FDA-approved cell-based therapy for cartilage defects involves autologous chondrocyte implantation: chondrocytes harvested from low-contact areas are expanded in vitro and then re-injected directly into the damaged site 1 . (nature.com)

Optimizing CO2 normalizes pH and enhances chondrocyte viability during cold storage. (nih.gov)
Viability and volume of in situ bovine articular chondrocytes-changes following a single impact and effects of medium osmolarity. (dojindo.com)
The inhibitory doses had no adverse effect on the viability of chondrocytes. (aspetjournals.org)
Compared with intact cored discs, dicing of AC tissue into 0.2-1 mm cubes significantly increased the viability and proliferative capacity of surviving chondrocytes after cryopreservation. (ox.ac.uk)
The effect of hyaluronidase treatment on GAG content, chondrocyte viability, and biochemical composition of the extracellular matrix was also determined. (cardiff.ac.uk)
This hypothesis was tested by impacting bovine cartilage explants with nominal stresses ranging from 0.5 to 65 MPa and measuring proteoglycan biosynthesis, cell viability, and water content immediately after impaction and 24 hours later. (lievers.net)
Matrix damage and chondrocyte viability following a single impact load on articular-cartilage. (lievers.net)

In vivo effects of single intra-articular injection of 0.5% bupivacaine on articular cartilage. (nih.gov)
Intra-articular injection of hyaluronic acid (HA) or corticosteroids was also shown to improve pain, whereas the HA administration showed more prolonged effect [ 10 , 11 ]. (biomedcentral.com)

The purpose of this study was to examine the effects of PRP on cell proliferation and matrix synthesis by porcine chondrocytes cultured in alginate beads, conditions that promote the retention of the chondrocytic phenotype, in order to determine the plausibility of using this plasma-derived material for engineering cartilage. (qxmd.com)
Primary bovine intervertebral disc cells transduced with adenovirus overexpressing 12 BMPs and Sox9 maintain appropriate phenotype. (uchicago.edu)
The conventional approach utilized to induce dedifferentiation in chondrocytes are comprised of adding dedifferentiating agents or techniques which are often highly expensive, require specific conditions and a long time period to convert the chondrocyte phenotype into fibroblastic cells 8 . (researchsquare.com)
This study provides evidence for further testing on the molecular mechanism of the disease and also propose proteins as candidate markers of OA chondrocyte phenotype. (biomedcentral.com)
Notochordal cell conditioned medium (NCCM) regenerates end-stage human osteoarthritic articular chondrocytes and promotes a healthy phenotype. (unibas.ch)

So, it is of imminent importance to study the molecular process of chondrocytes differentiation and dedifferentiation in order to discover potential therapies to manipulate the activity to rehabilitate chondrogenic functions and morphology to prevent degeneration of articular joints in various cartilage associated diseases. (researchsquare.com)
HE staining and iron red solid green staining were used to observe the degeneration of articular chondrocytes in rats. (researchsquare.com)

Cells were isolated from the nucleus of bovine discs and from bovine articular cartilage, embedded in alginate beads and cultured at pH levels maintained within the ranges seen in normal and pathological discs: pH 7.4-pH 6.3 for 48 h. (ox.ac.uk)

Methods: Bovine articular chondrocytes were expanded and transferred into pellet culture at passage 3. (bvsalud.org)
METHODS: Collected media from bovine cartilage explant cultures stimulated with IL-1+OSM were subjected to anion-exchange chromatography. (ox.ac.uk)
This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. (ox.ac.uk)
The methods used to induce dedifferentiation in cell models of chondrocytes are often time consuming or require expensive reagents. (researchsquare.com)
Whereas we have utilized 2-deoxy-D-glucose as a dedifferentiating agent which is relatively cheap and quickly induces dedifferentiation in chondrocytes as compared to other methods 9 . (researchsquare.com)
In addition, the composition and structure of articular cartilage were investigated with multiple reference methods involving light microscopy, digital densitometry, polarized light microscopy and Fourier infrared imaging. (jukkaliukkonen.fi)
Reference methods indicated that acoustic anisotropy of ultrasound scattering arises mainly from non-uniform distribution of chondrocytes and anisotropic orientation of collagen fibers. (jukkaliukkonen.fi)

PRP isolated from autologous blood may be useful as a source of anabolic growth factors for stimulating chondrocytes to engineer cartilage tissue. (qxmd.com)
Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O,Peterson L: Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. (mikkolammi.com)
Nasal chondrocyte-based engineered autologous cartilage tissue for repair of articular cartilage defects: an observational first-in-human trial. (unibas.ch)

These mechanical loads are absorbed by the cartilage extracellular matrix (ECM), where they are subsequently dissipated and transmitted to chondrocytes (cartilage cells). (wikipedia.org)
The effect of extracellular pH on matrix turnover by cells of the bovine nucleus pulposus. (ox.ac.uk)
Mouse and bovine primary cells are available for basic research. (criver.com)
In equine chondrocytes, NHE is inhibited when cells are incubated for 3 h at low O(2), leading to intracellular acidosis. (ox.ac.uk)
The present study examines whether hypoxia can influence chondrocyte NHE activity and pH(i) over shorter timescales using the pH-sensitive fluoroprobe BCECF in cells isolated not only from equine cartilage but also from bovine tissue. (ox.ac.uk)
Biochemical analyses revealed that treatment with PRP growth factors did not markedly affect the types of PGs and collagens produced by porcine chondrocytes, suggesting that the cells remained phenotypically stable in the presence of PRP. (qxmd.com)
Bovine endometrial cells were cultured until passage (P) 10 with 5 % or 10 % PRP. (biomedcentral.com)
Further studies are required to determine whether these cells may retain cartilage differentiation capacity and provide sufficient chondrocytes for use as implants in clinical applications. (ox.ac.uk)
Of particular interest is an injectable, in situ gelling hydrogel for cell encapsulation that can be delivered with low cytotoxicity to native chondrocytes and encapsulated cells. (queensu.ca)
Passage 0 chondrocyte migration was less than 3% of seeded cells for all conditions investigated with no significant differences between groups. (queensu.ca)
Comparative effects of bone morphogenetic proteins and Sox9 overexpression on matrix accumulation by bovine anulus fibrosus cells: implications for anular repair. (uchicago.edu)
Comparative effects of bone morphogenetic proteins and sox9 overexpression on extracellular matrix metabolism of bovine nucleus pulposus cells. (uchicago.edu)
Chondrocytes are derived from mesenchymal cells through the process of differentiation during embryogenesis. (researchsquare.com)
However, in various cartilage degenerative diseases, the chondrocytes transition itself to more flattened fibroblast cells with different morphological, genetical and biochemical characteristics which is characterized as dedifferentiation 4,5 . (researchsquare.com)
Outcomes Arousal of bovine NP cells by both IGF-1 and BMP7 significantly potentiates anabolism through complementary and synergistic systems on matrix development in comparison with treatment with either development factor by itself. (researchassistantresume.com)
Alternatively IGF-1 considerably suppresses noggin manifestation the PI3K/Akt pathways and therefore potentiating BMP7 signaling in bovine NP cells. (researchassistantresume.com)
BMP7 has similar anabolic effects by stimulating matrix biosynthesis in human adult articular chondrocytes (13) bovine IVD cells (14) rabbit IVD cells (15) and human IVD cells (16). (researchassistantresume.com)
Osada and colleagues showed that IGF-1 stimulates PG synthesis in bovine NP cells in serum-free conditions in a dose-dependent manner and proposed an autocrine/paracrine mechanism of action (10). (researchassistantresume.com)
Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
Bovine cartilage explants were treated with hyaluronidase to deplete GAG content and then seeded with fluorescently tagged human endothelial cells (HMEC-1). (cardiff.ac.uk)
Our results support the hypothesis that articular cartilage GAGs are antiadhesive to endothelial cells and suggest that chondroitin sulfate and/or hyaluronan are responsible. (cardiff.ac.uk)
Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. (nature.com)

The first tissue-engineered, ear-shaped appendages made from bovine chondrocytes and biocompatible scaffolds by the Vacanti group were prone to deformation when xenografted onto immune-compromised mice, highlighting the lack of long-term stability [ 6 ]. (biomedcentral.com)
OBJECTIVE: In a previous study, we identified a 50-kDa G3-containing aggrecan degradation product in bovine cartilage, released from the tissue after interleukin-1 (IL-1) stimulation in the presence of oncostatin M (OSM). (ox.ac.uk)
The bovine endometrium is a complex tissue that comprises the tunica mucosa (lamina epithelialis and lamina propria consisting of loose connective tissue) and the tunica submucosa of the uterus. (biomedcentral.com)
In conclusion, modification of appropriate factors, such as the size of the tissue, cryoprotective agent, and isolation protocol, can allow successful isolation of viable chondrocytes with high proliferative capacity from cryopreserved human articular cartilage tissue. (ox.ac.uk)
Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. (columbia.edu)
Regenerative Potential of Tissue-Engineered Nasal Chondrocytes in Goat Articular Cartilage Defects. (unibas.ch)

Here, we show that in primary human femoral head osteoarthritic and normal bovine chondrocytes, TWHF partially or completely inhibited mRNA and protein expression of tumor necrosis factor-α, interleukin (IL)-1, and IL-17-inducible MMP-3 and MMP-13. (aspetjournals.org)
We performed a quantitative proteomic analysis in osteoarthritic and normal chondrocytes followed by functional analyses to investigate proteome changes and molecular pathways involved in OA pathogenesis. (biomedcentral.com)
In this study we compared the quantitative changes in proteins synthesized in osteoarthritic compared to normal chondrocytes. (biomedcentral.com)

Histologic and immunohistochemical evaluations indicated a higher differentiation potential of chondrocytes treated with 84-EVi. (bvsalud.org)
BMP7 a member of the transforming growth factor-β (TGF-β) superfamily is expressed in cartilage and exerts potent anabolic effects by stimulating differentiation and metabolic functions of both osteocytes and chondrocytes (12). (researchassistantresume.com)

The conserved nature and rapidity of the response to hypoxia has considerable implications for chondrocyte homeostasis and potentially for the maintenance of cartilage integrity. (ox.ac.uk)

Chondrocyte dedifferentiation is a cellular phenomenon in which chondrocyte loses its chondrogenic characteristics and ability to synthesize extracellular matrix. (researchsquare.com)
The differentiated chondrocyte is capable of synthesizing and regulating the extracellular matrix such as collagen type II, proteoglycans which are responsible for cartilage development and repair 1-3 . (researchsquare.com)

Influence of medium composition, static and stirred conditions on the proliferation of and matrix protein expression of bovine articular chondrocytes cultures in 3-D collagen scaffold. (novotec-labs.com)
Collagen, proteoglycans and chondrocytes can contribute to ultrasound scattering in articular cartilage. (jukkaliukkonen.fi)
The biomechanical function of the collagen fibril ultrastructure of articular cartilage. (lievers.net)
In Paper III, we developed and validated a robust and specific ELISA assay measuring a MMP-1 and MMP-13 cleaved type II collagen fragment (T2CM) that we identified in the study for Paper I. It was elevated in response to pro-inflammatory cytokine treatment of bovine articular cartilage, and in osteoarthritis patients referred for a total knee joint replacement surgery compared to patients with moderate or severe disease. (lu.se)

This pilot study aimed to investigate the effect of characterized MSC-EVs on chondrogenesis in a 3D chondrocyte inflammation model with the pro-inflammatory cytokine TNFα. (bvsalud.org)
The major pathologic manifestations of rheumatoid arthritis (RA) and osteoarthritis (OA) are joint inflammation and articular cartilage resorption by proinflammatory cytokine-stimulated matrix metalloproteinases (MMPs) and aggrecanases. (aspetjournals.org)
In Paper I we investigated the effect of pro-inflammatory cytokine interleukin-17 on articular cartilage in a bovine explant model system. (lu.se)
We also found that interleukin-17A can upregulate other pro-inflammatory cytokine release from chondrocytes and a release of both proteins and protein metabolism fragments into conditioned media. (lu.se)

Specifically we assessed the biological and mechanistic effects of co-administering BMP7 and IGF-1 on cartilage homeostasis using bovine IVD as a pre-translational model. (researchassistantresume.com)
Dysregulated cartilage homeostasis, articular cartilage degradation and abnormal bone remodeling are common denominators in joint disease and can lead to severe disability for the patients. (lu.se)

Chondrocyte intracellular pH (pH(i)) is a powerful modulator of matrix synthesis and is principally regulated by Na(+)-H(+) exchange (NHE). (ox.ac.uk)
IGF-1 is a single chain polypeptide that is Idasanutlin (RG7388) structurally similar to insulin a key growth factor that enhances PG synthesis in articular cartilage (17). (researchassistantresume.com)

Rapid effects of hypoxia on H+ homeostasis in articular chondrocytes. (ox.ac.uk)

Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (CPA). (ox.ac.uk)

During the progression of FJOA, these pro-inflammatory cytokines are considered to be key factors in promoting chondrocyte apoptosis. (researchsquare.com)

Objective To assess therapeutic benefits for intervertebral disc matrix repair and regeneration the synergism of IGF-1 and BMP7 in Idasanutlin (RG7388) bovine spine discs were evaluated and molecular/mobile mechanisms were elucidated. (researchassistantresume.com)

Cell therapy using articular chondrocytes overexpressing BMP-7 or BMP-10 in a rabbit disc organ culture model. (uchicago.edu)
Here in our protocol, we describe the utility of using 2-deoxy-D-glucose as a dedifferentiating agent for rabbit chondrocytes for studying dedifferentiation process. (researchsquare.com)
For this purpose, various cell models are deployed such as chicken, bovine, rat and rabbit, chondrocytes as they are analogues to human chondrocytes 7 . (researchsquare.com)

Most previous studies have evaluated single proteins, but have not addressed the total chondrocyte proteome. (biomedcentral.com)
We analyzed the proteome of chondrocytes using high resolution mass spectrometry and quantified it by label-free quantification and western blot analysis. (biomedcentral.com)

Articular cartilage undergoes severe loss of proteoglycan and its constituent glycosaminoglycans (GAGs) in osteoarthritis. (cardiff.ac.uk)

With the aim to describe more clearly the effects of GS and CS on cartilage biology and characterize their mechanism of action, we performed proteomic analyses of articular chondrocytes treated with exogenous GS and/or CS. (biomedcentral.com)
Pour aller toujours plus loin dans la caractérisation des tissus, des hydrogels et des cellules, Novotec propose des analyses en Microscopie Electronique à Transmission : préparation des échantillons (inclusion en résine, coupes semi-fines et ultrafines. (novotec-labs.com)

Adult porcine chondrocytes were cultured in the presence of 10% PRP, 10% PPP or 10% fetal bovine serum (FBS) for 3 days. (qxmd.com)
Fetal bovine aggrecan was digested with full-length recombinant ADAMTS-4 and ADAMTS-5 and resulting degradation products were analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE) and immunoblotting using an anti-G3 antiserum and an anti-neoepitope antibody that had been generated to the new N-terminus of the G3 fragment. (ox.ac.uk)

Hypertonic conditions enhance cartilage formation in scaffold-free primary chondrocyte cultures. (mikkolammi.com)

CS and GS differentially modulate the proteomic profile of human chondrocytes. (biomedcentral.com)

Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis. (qxmd.com)

For equine and bovine cell populations subjected to step change in O(2) by resuspension in hypoxic (1%) solutions, a decline in acid extrusion and pH(i) was observed within 10 min and continued throughout the recording period. (ox.ac.uk)

This pharmacoproteomic approach unravels the complex intracellular mechanisms that are modulated by these drugs on IL1β-stimulated human articular chondrocytes. (biomedcentral.com)

so perhaps on the proximal end of the phalanx bone there may be a periosteal membrane despite being separated by articular cartilage. (heightquest.com)

Here we show, in articular chondrocytes, that IL-1β-induces primary cilia elongation with alterations to cilia trafficking of arl13b. (biomedcentral.com)
Chondrocytes from OA patients were used in primary culture and stimulated with 10 ng/ml interleukin(IL)-1β in the presence or absence of AD-MSC microvesicles, exosomes or conditioned medium. (karger.com)
Chondrocytes were isolated from articular cartilage of ten patients with primary OA undergoing knee replacement surgery and six normal donors undergoing fracture repair surgery without history of joint disease and no OA clinical manifestations. (biomedcentral.com)

Chemotaxis of passage 0 and passage 2 bovine chondrocytes toward bovine ASCs was measured using a modified Boyden chamber assay. (queensu.ca)

A method of isolating viable chondrocytes with proliferative capacity from cryopreserved human articular cartilage. (ox.ac.uk)
In this protocol, we utilized 2 weeks old New Zealand rabbits to acquire articular chondrocyte that offers more genetic resemblance to human chondrocyte and can be used to accurately study the molecular mechanism behind cartilage degeneration. (researchsquare.com)
Loeser and colleagues noted that neither BMP7 nor IGF-1 alone are mitogenic in human adult articular cartilage but BMP7 and IGF-1 together may modestly increase chondrocyte number and PG accumulation (19). (researchassistantresume.com)

We identified several pathways and proteins to be associated with OA chondrocytes. (biomedcentral.com)

Chondrocytes sense and convert the mechanical signals they receive into biochemical signals, which subsequently direct and mediate both anabolic (matrix building) and catabolic (matrix degrading) processes. (wikipedia.org)
Osteoarthritis (OA) is a multi-factorial disease leading progressively to loss of articular cartilage and subsequently to loss of joint function. (biomedcentral.com)

Besides the cell source choice, also mechanical stimuli are crucial in the development and maintenance of articular cartilage. (nature.com)

These results reinforce the notion that, at early time points in culture, hydrodynamic shear below 10 mPa may promote formation of extra-cellular matrix specific to hyaline cartilage in chondrocyte-seeded constructs. (iusspavia.it)

While hypertrophy of chondrocytes is a physiological process implicated in the longitudinal growth of long bones, hypertrophy-like alterations in chondrocytes play a major role in OA. (biomedcentral.com)

Due to its unique location at joint surfaces, articular cartilage experiences a range of static and dynamic forces that include shear, compression and tension. (wikipedia.org)
Osteochondral samples were obtained from intact bovine patellas, and cartilage was imaged in two perpendicular directions: through articular and lateral surfaces. (jukkaliukkonen.fi)

Anatomy21

Organisms4

Diseases8

Chemicals and Drugs65

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes24

Disciplines and Occupations1

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Information Science2

Vitro9

Viability7

Intra-articular2

Phenotype5

Degeneration of articular2

Embedded in alginate beads1

Methods7

Autologous3

Cells22

Tissue6

Osteoarthritic3

Differentiation2

Implications for chondrocyte1

Extracellular2

Collagen4

Cytokine4

Cartilage homeostasis2

Synthesis2

Homeostasis1

Osteoarthritis patients1

Apoptosis1

Intervertebral Disc1

Rabbit3

Proteome2

Proteoglycan1

Analyses2

Fetal2

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Proteomic1

Stimulates1

Equine1

Intracellular1

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Human3

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