PorcineViralRecombinant adenovirusCaprineVectorsCanine disteRespiratoryImmunizationReplicationSheepResearchersGene deliveryCellsProteinsVirusSystemFigureWebsiteHerpesvirusNovel adenovirusRespiratorySerotypesHerpes virusHelper virusDetectionSpeciesEpidemiologyVirusesDeerStrainRuminantLiverCattleHumanOriginally
Porcine1
- Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, and porcine IL-7. (bio-techne.com)
Viral5
- Maedi-visna (MV), visna/maedi or ovine progressive pneumonia (OPP) is a chronic viral disease of sheep and goats with worldwide prevalence. (cabi.org)
- Due to the induction of strong innate and adaptive immune responses and limited the duration of transgene expression of first-generation adenovirus vector, researchers have further developed the second-generation adenovirus vectors by deleting the E2 or E4 coding sequences, resulting in some beneficial effects of reducing the expression of viral proteins and vector-directed immune responses. (genemedi.com)
- Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus. (9lb.cc)
- The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. (9lb.cc)
- E1A is critical to start viral replication by promoting transcription from rep gene promoters, P5 and P19, and facilitate viral replication by activating the early adenovirus promoters. (9lb.cc)
Recombinant adenovirus9
- Recombinant adenovirus type 5 (rAd) has been used as a vaccine platform against many infectious diseases and has been shown to be an effective vaccine vector. (mdpi.com)
- Traditionally, recombinant adenovirus vectors used in gene delivery were prepared with a plasmid containing the transgene flanked by inverted terminal repeats (ITRs), co-transfected with packaging plasmid pAd-BHGlox(delta)E1, E3. (genemedi.com)
- The current method of the recombinant adenovirus production is based on Adeasy and Ad.MAX™ system. (genemedi.com)
- The two plasmids co-transfection system of recombinant adenovirus. (genemedi.com)
- Recombinant adenovirus (Adenovirus), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. (9lb.cc)
- We also show that recombinant adenovirus vaccination induced T cell responses to the same epitopes, and led to memory T cell differentiation. (biomedcentral.com)
- T cells primed by these recombinant adenovirus vaccines expanded after PPRV challenge and probably contributed to protection. (biomedcentral.com)
- These data validate the use of recombinant adenovirus expressing PPRV genes as DIVA strategies to control this highly contagious disease. (biomedcentral.com)
- Recombinant adenovirus vaccines should therefore aim at replicating the naturally occurring PPRV immunity. (biomedcentral.com)
Caprine1
- Bovine IFN-τ is N-glycosylated at ASN78, caprine IFN-τ is a combination between nonglycosylated and glycosylated forms and ovine IFN-τ is not glycosylated. (wikipedia.org)
Vectors3
- To date, there have been many different generations of adenovirus vectors, differing in the extent to which the genome from wild-type adenovirus is attenuated, among them, most studies involving adenovirus vectors utilize the simple E1-deleted, first-generation adenovirus vector. (genemedi.com)
- The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can't be integrated into host genome, guaranteeing the security for subsequent operations. (9lb.cc)
- Transduction results of various Adenovirus vectors. (9lb.cc)
Canine diste1
- Immunohistochemical and molecular assays designed to identify common infectious disease pathogens of dogs did not confirm the participation of canine distemper virus, canine parvovirus, or canine adenovirus in the target tissues of this dog. (bvsalud.org)
Respiratory1
- Deep nasal swabs (DNS) from asymptomatic calves, calves with pulmonary discomfort, and diarrheic calves after episodes of respiratory distress were used in molecular assays designed to detect the principal pathogens associated with bovine respiratory disease (BRD). (bvsalud.org)
Immunization1
- Immunization strategies against this morbillivirus using recombinant adenoviruses expressing PPRV-F or -H proteins can protect PPRV-challenged animals and permit differentiation of infected from vaccinated animals. (biomedcentral.com)
Replication3
- Since wild-type adenovirus is associated with a wide range of illnesses and enlists a variety of immune responses, so recombinant replication deficiency virus has been an attractive vector for gene therapy [20]. (genemedi.com)
- The E1 gene contains E1A and E1B, involved in the replication of adenovirus. (9lb.cc)
- AAV belongs to the Parvoviridae family and Dependovirus genus, whose members require co-infection with a helper virus such as adenovirus to promote replication, and AAV establishes a latent infection in the absence of a helper. (justia.com)
Sheep4
- by the sublin- on the coding region between Aichi TCA-3 corresponding to nt 7518- eage position of the sheep strain on (strain A846/88), bovine (U-1), and 7540 of bovine kobuvirus) ( 4 ). (cdc.gov)
- Reaction Kit (Applied Biosystems, 2 potential ruminant hosts, cattle and Aichi virus and bovine kobuvirus Warrington, UK) with the PCR prim- sheep. (cdc.gov)
- The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. (ac.ir)
- A BETARETROVIRUS that causes pulmonary adenomatosis in sheep ( PULMONARY ADENOMATOSIS, OVINE ). (lookformedical.com)
Researchers1
- Genemedi has launched a comprehensive adenovirus production service, including a variety of different adenoviral expression systems used for gene over-expression and shRNA mediated gene down-regulation, which may also allow researchers to produce adenoviruses by themselves as they need. (9lb.cc)
Gene delivery1
- Adenovirus packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. (9lb.cc)
Cells3
- IFN-τ is constitutively secreted by trophoblast and endometrial cells during ovine pregnancy. (wikipedia.org)
- Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues. (9lb.cc)
- Selected results of Adenovirus transduction in the adult rat cardiac cells (A), eyes (B), neuron cells (C). (9lb.cc)
Proteins1
- CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (bio-techne.com)
Virus1
- 2018) Valoración de un nuevo ensayo quimioluminiscente en comparación con ELISA en la detección de IgG contra el virus de la hepatitis E. Revista de la Sociedad Andaluza de Microbiología y Parasitología Clínica. (vircell.com)
System1
- Synthetic gene for ovine IFN-τ was produced using Pichia pastoris yeast system. (wikipedia.org)
Figure1
- These primers were to bovine kobuviruses (Figure). (cdc.gov)
Website1
- Adenovirus User Manual on the Genemedi website. (9lb.cc)
Herpesvirus1
- One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3. (zoologix.com)
Novel adenovirus1
- Our data demonstrate that we have successfully vectorized a novel adenovirus type candidate with low seroprevalence. (bvsalud.org)
Respiratory2
- Ultrasensitive detection of bovine respiratory syncytial virus by reverse transcription coupled real time PCR. (zoologix.com)
- Bovine respiratory syncytial virus (BRSV) is an RNA virus belonging to the paramyxoviridiae family. (zoologix.com)
Serotypes1
- A species of adenovirus comprising Bovine adenovirus serotypes 4-8. (nih.gov)
Herpes virus1
- As its name implies, AAV is dependent upon the presence of a helper virus, such as an adenovirus or herpes virus, for active replication. (justia.com)
Helper virus1
- AAV belongs to the Parvoviridae family and Dependovirus genus, whose members require co-infection with a helper virus such as adenovirus to promote replication, and AAV establishes a latent infection in the absence of a helper. (justia.com)
Detection1
- In order to test the primers and the method for the detection of the causative agent of the Rubarth's disease of dogs (canine adenovirus type 1, CAV-1) in the infected liver tissues we elaborated a simple, effective sample processing method and used it successfully not only for the exposition of CAV-1 DNA, but for goose parvovirus (GPV) DNA or mycoplasma genomic DNA as well in the ensuing protocols. (huveta.hu)
Species4
- The type species is Ovine adenovirus D. (nih.gov)
- To assess the prevalence of adenoviruses in psittacine birds kept in Slovenia, 258 cloacal swabs were collected from different psittacine species and screened by a nested PCR with degenerate, consensus primers targeting the adenoviral DNA polymerase gene. (bvsalud.org)
- By sequencing, 28 samples from 10 different parrot species were identified as the formerly described siadenovirus, psittacine adenovirus 2 (PsAdV-2). (bvsalud.org)
- Human adenovirus species D (HAdV-D) types are currently being explored as vaccine vectors for coronavirus disease 2019 (COVID-19) and other severe infectious diseases. (bvsalud.org)
Epidemiology1
- Epidemiology and control of bovine spongiform encephalopathy (BSE. (microbiologyresearch.org)
Viruses1
- Adenoviruses are DNA viruses that naturally infect many vertebrates, including humans and monkeys, and cause a wide range of clinical illnesses in humans. (nih.gov)
Deer1
- The PrP gene encodes the putative causative agent of the transmissible spongiform encephalopathies (TSEs), a heterogeneous group of fatal, neurodegenerative disorders including human Creutzfeldt-Jakob disease, bovine spongiform encephalopathy, ovine scrapie and chronic wasting disease (CWD) of North American deer and elk. (microbiologyresearch.org)
Strain2
Ruminant1
Liver1
- A total of 108 bovine liver samples ended up cut having a diode laser beam from various configurations within pulsed and also micropulsed setting as well as histologically assessed to ascertain the place as well as degree associated with carbonization, necrosis as well as reversible injury, as well as incision detail and size. (hif-signals.com)
Cattle1
- The lineage) with the kobuvirus strains in and Bovine kobuvirus ( 2 ), and 1 can- continuous 3D and 3 untranslated cattle. (cdc.gov)
Human1
- Here, we describe the discovery and vectorization of a chimeric human adenovirus, which we call HAdV-20-42-42. (bvsalud.org)
Originally1
- IFN-τ was first discovered in ruminants as the signal for the maternal recognition of pregnancy and originally named ovine trophoblast protein-1 (oTP-1). (wikipedia.org)