Bound to concanavalin. Medical search. Frequent questions

Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean (Canavalia ensiformis). (wikipedia.org)
Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. (vectorlabs.com)
I purchased an agarose bound lectin from Vector Labs. (vectorlabs.com)
Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
b) self-assembly of sugar-decorated grid-shaped metal complexes, able to bind multiple lectins (sugar-binding proteins) with concomitant formation of hybrid biopolymers (hierarchical self-assembly where cation binding is followed by sugar-lectin interaction). (europa.eu)
Metabolic labeling of immature jackbean cotyledons with 14C-amino acids was used to determine the processing steps involved in the assembly of concanavalin A. Pulse-chase experiments and analyses of immunoprecipitated lectin forms indicated a complex series of events involving seven distinct species. (rupress.org)
NH2-terminal sequencing of the abundant precursors confirmed that the intact subunit of concanavalin A was formed by the reannealing of two fragments, since the alignment of residues 1-118 and 119-237 was reversed in the final form of the lectin identified in the chase and the precursor first labeled. (rupress.org)
This processing activated the carbohydrate-binding activity of the lectin. (rupress.org)
Rapidly purify lectins, galactose-binding molecules, agglutinins, N-acetylgalactosamine-binding, N-acetylglucasomine-binding and carbohydrate binding molecules with our lectin purification reagents. (gbiosciences.com)
The rat enzyme, however, is heavily glycosylated and binds to wheat-germ lectin-Sepharose 6MB and 5-hydroxytryptamine-agarose, but not to concanavalin A-Sepharose 4B. (wikigenes.org)
Concanavalin A is a lectin found in Canavelia that exhibits immunostimulatory, pro-fibrotic, and anticancer chemotherapeutic activities. (lktlabs.com)
Lectin of Concanavalin A as an anti-hepatoma therapeutic agent. (lktlabs.com)
Noonan KD, Burger MM. The relationship of concanavalin A binding to lectin-initiated cell agglutination. (lktlabs.com)
Concanavalin A is a unique lectin purified from the jack bean, C. ensiformis, that selectively cross-links cell-surface glycoproteins and affects the initiation of cell agglutination, mitogenesis, and apoptosis. (bertin-bioreagent.com)
ConA is a lectin (carbohydrate-binding protein) that binds specifically to mannosyl- and glucosyl-containing extracellular glycoproteins. (tebubio.com)
The researchers of this study initially synthesised a mannose (a type of sugar) derivative of DTC to study its binding properties with the lectin, concanavalin. (researchmatters.in)
A similar result was observed when galactose (mannose but with a different arrangement of atoms) bound DTC reacted with the galactose-specific lectin, wheat germ agglutinin. (researchmatters.in)

Concanavalin A (Con A) affinity chromatography is the standard procedure to separate cholesterol nucleating biliary proteins from lipids and pigment. (wustl.edu)
Lipid radioactivity was associated with Con A-binding proteins prepared without taurocholate, but not in those prepared with taurocholate wash. (wustl.edu)
A number of inert ligands which were not expected to react with nor bind to any of the targeted enzymes or proteins were also included on the chip for comparison (see figure 2). (uchicago.edu)
After dipping the patterned chip in several solutions of different proteins or enzymes, the researchers observed that, in each case, selective binding or selective reaction occurred on the biochip. (uchicago.edu)
Nucleotide-binding oligomerization domain proteins (NODs) are modular cytoplasmic proteins implicated in the recognition of peptidoglycan-derived molecules. (hindawi.com)
Calmodulin resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells are available as resin only or in kit form including binding and elution buffers. (gbiosciences.com)
Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. (gbiosciences.com)
These findings provide additional evidence that calcium-binding EF-hand proteins play roles in host-parasite interaction. (biomedcentral.com)
Tegumental proteins (TegPs) belong to a family of calcium-binding proteins (CaBPs), which contain two EF-hand motifs at the N-terminal domain and a C-terminal dynein light chain-like domain [ 7 ]. (biomedcentral.com)
The biochemical properties of CaBPs from different parasites have been investigated, and despite the considerable resemblance between structural models of all family members, differences exist in the dimerization, ion-binding and drug-binding characteristics of these apparently similar proteins [ 14 ]. (biomedcentral.com)
Cleavage Under Targets and Tagmentation (CUT&Tag) is a method to investigate genomic localization of histone modifications and some transcription factors that reveals interactions between proteins and DNA or identifies DNA binding sites for proteins of interest. (epigenie.com)
Unlike MNase-Seq or ATAC-Seq methods that target open chromatin and are therefore dependent on chromatin accessibility, CUT&Tag utilizes an antibody-based enzyme tethering strategy to target specific histone modifications or proteins to reveal chromatin-binding information that is specific to those sites or proteins of interest. (epigenie.com)
Uncover the Correct Stain for Your Utility Con A and completely different lectins are carbohydrate binding proteins that acknowledge specific sugar moieties on glycoproteins. (swissprot20.org)
Proteins that share the common characteristic of binding to carbohydrates . (lookformedical.com)
Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates , however they are not considered lectins . (lookformedical.com)
PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). (lookformedical.com)
They have discovered a novel property of a class of compounds called dithiocarbamates (DTC), which act as a light switch whenever they bind to specific proteins called lectins. (researchmatters.in)
Lectins are proteins that play the role of connectors by binding to the surface sugars of two cells and aiding the exchange or transport of molecules. (researchmatters.in)
Furthermore, molecular docking demonstrated that the binding fraction of G13 with these proteins was high. (bvsalud.org)
To make proteins, such as binding, recognition and catalysis, often progress, it is therefore helpful to adopt a sceptical atti- involve specific interactions with individual water mol- tude. (lu.se)

Like most lectins, ConA is a homotetramer: each sub-unit (26.5kDa, 235 amino-acids, heavily glycated) binds a metallic atom (usually Mn2+ and a Ca2+). (wikipedia.org)
Agarose bound Ricinus communis agglutinin I is prepared using our affinity-purified lectins. (vectorlabs.com)
Ligands resembling streptavidin, antibodies and lectins are used every to grab specifically-tagged targets and for the isolation of cells and biomolecules that naturally categorical the ligand binding companion. (isotope.info)
The distinctive saccharide-binding properties of plant lectins, resembling Concanavalin A (Con A) have made them useful for the labeling and isolation of glycanpresenting cells and glycoproteins in serum and cell lysate. (isotope.info)
Several lectins like concanavalin (ConA) and wheat germ agglutinin (WGA), from both plants and animals, have been identified and studied. (researchmatters.in)
This discovery would help to visualise the binding properties of lectins in a much easier way and thus open new doors to probe the salient role of lectins in biological systems. (researchmatters.in)

One column was washed in the usual manner with Tris-HCl buffer and the other with buffer containing 10 mM taurocholate prior to eluting bound glycoproteins with α-D-methylmannopyranoside. (wustl.edu)
It binds specifically to certain structures found in various sugars, glycoproteins, and glycolipids, mainly internal and nonreducing terminal α-D-mannosyl and α-D-glucosyl groups. (wikipedia.org)
Internalization of ConA occurs preferentially to the mitochondria after binding to cell membrane glycoproteins, which triggers an autophagic cell death. (wikipedia.org)
it binds α-D-mannosyl and α-D-glucosyl residues on sugars, glycoproteins, and glycolipids. (lktlabs.com)
23899} Concanavalin A specifically binds to α-mannose and α-galactose structures found in sugars, glycoproteins, and glycolipids and has been used in affinity chromatography purifications of various glycoproteins and cellular structures. (bertin-bioreagent.com)
Many of these minor bands are accounted for by glycoproteins, as revealed by their binding of 125I-concanavalin A, and two of these are also stained blue by the stains-all procedure, a reaction specific for acidic glycoconjugates. (rupress.org)

ConA binds specifically α-D-mannosyl and α-D-glucosyl residues (two hexoses differing only in the alcohol on carbon 2) in terminal position of ramified structures from B-Glycans (rich in α-mannose, or hybrid and bi-antennary glycan complexes). (wikipedia.org)
PHA and ConA bind and cross-link components of the T cell receptor, and their ability to activate T cells is dependent on expression of the T cell receptor. (wikipedia.org)
This product contains Concanavalin A (ConA) conjugated to paramagnetic microspheres. (tebubio.com)

Light microscopy revealed the presence of cholesterol microcrystals and vesicles in some Con A-binding protein preparations prepared without a taurocholate wash. (wustl.edu)
concanavalin A was used as a model protein) and each concanavalin A can bind up to four grids. (europa.eu)
Involvement of water in carbohydrate-protein binding: concanavalin A revisited. (expasy.org)
The Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) has recently emerged as a leading candidate antigen against the blood-stage human malaria parasite. (nature.com)
High affinity biotin tagged protein resins are available along with streptavidin binding and elution buffers and reagents for the quantitation of biotin . (gbiosciences.com)
It has been reported that FR70 antibody blocks binding of mCD27-Ig fusion protein to mouse Cd70-transfected cells and inhibits some in vitro T-cell- and NK-cell-dependent responses. (bdbiosciences.com)
In this study, we investigated the immunomodulatory effects of a recombinant tegumental calcium-binding EF-hand protein 4 of F. gigantica (rFg-CaBP4) on goat monocytes. (biomedcentral.com)
Directly following antibody binding, the chromatin is digested and NGS libraries are prepared in a single step by tagmentation using the protein A-Tn5 (pA-Tn5) transposome enzyme that has been pre-loaded with sequencing adapters. (epigenie.com)
7: Marks A, Labourdette G. Succinyl concanavalin A stimulates and antimicrotubular drugs inhibit the synthesis of a brain-specific protein in rat glial cells Proc Natl Acad Sci U S A. 1977 Sep;74(9):3855-8. (moleculardepot.com)
protein_coding" "Cz02g32120.t1","No alias","Chromochloris zofingiensis","B-block binding subunit of TFIIIC [Interproscan]. (ntu.edu.sg)
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. (lookformedical.com)
Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein. (academicinfluence.com)
The high mobility of hydration water ensures that all thermally activated processes at the protein-water interface, such as binding, recognition and catalysis, can proceed at high rates. (lu.se)

All variants bound the PfRH5 receptor basigin and were recognized by a panel of monoclonal antibodies. (nature.com)

Versatile synthetic route has been developed to novel ligands possessing both cation and anion binding sites. (europa.eu)
To produce simple model system having these characteristics, we have designed and synthesised sugar-decorated ligands able to self-assemble into grid-like complexes upon binding with transition metal cations (so-called [2x2] grids, comprising 4 ligands and 4 cations). (europa.eu)
Most significantly, under specific conditions one of the grids was shown to agglutinate concanavalin A, whereas neither its ligands nor their components could do so. (europa.eu)
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. (lookformedical.com)

PGL-1 from M. leprae inhibits the proliferation of T lymphocytes after stimulation with concanavalin A ( 2 ). (cdc.gov)
Flow cytometer analyses demonstrated an increased frequency following the stimulation with Concanavalin A. The frequency of mAb 4-13- and mAb 4-58-positive cells matched the total frequency of CD4- and CD8-positive cells. (uni-muenchen.de)
In durchflusszytometrischen Analysen steigt die Frequenz der positiven Zellen nach Stimulation mit Concanavalin A an. (uni-muenchen.de)
mRNA coding for CD70 is transiently expressed after Concanavalin A stimulation of splenocytes or thymocytes. (bdbiosciences.com)

This last feature may lead to hierarchical self-assembly, where self-assembled structure binds other molecules forming even larger structures representing higher level of complexity. (europa.eu)
In biological systems, many important processes are set off only after a 'precise binding' of two molecules. (researchmatters.in)

A variety of non-porous, high antibody binding capacity, cost effective magnetic beads are available. (gbiosciences.com)
The FR70 monoclonal antibody specifically binds to mouse CD70, a 30-33-kDa type-II transmembrane glycoprotein belonging to the TNF/NGF superfamily. (bdbiosciences.com)
Instead of the sonication of fixed chromatin and immunoprecipitation steps performed in ChIP-Seq protocols, in CUT&Tag, fresh (not frozen) unfixed cells are bound to concanavalin A beads and the antibody incubation is performed with cells in their native state. (epigenie.com)

Possibly they are binding to an equine CD45-molecule. (uni-muenchen.de)
Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule. (academicinfluence.com)

The concanavalin A receptor from human erythrocytes in lipid bilayer membranes. (moleculardepot.com)

Competitive fluorescence binding assays showed that RDP bound to ER with an IC10 of 0.26 µM, which is 20 folds lower than TPHP (5.6 µM). (bvsalud.org)

The percent inhibition of Concanavalin-A binding to polysaccharides was calculated from the absorbance value read at 420 nanometers. (cdc.gov)
Conversion of a hydroxyl group to an aldehydic or carboxyl function further enhances the binding inhibition effect of the compound. (cdc.gov)
5: Garrity P, Szelc C, Paquette C, McEvoy M, Millette R, Adler R. Inhibition of herpes simplex virus replication by succinyl concanavalin A Antimicrob Agents Chemother. (moleculardepot.com)
9: Kido N, Ohta M, Kato N. Inhibition by succinyl concanavalin A of strong adjuvant activity of lipopolysaccharides which possess mannans as the O-specific polysaccharide chains Cell Immunol. (moleculardepot.com)

Concanavalin A interacts with diverse receptors containing mannose carbohydrates, notably rhodopsin, blood group markers, insulin-receptor the Immunoglobulins and the carcino-embryonary antigen (CEA). (wikipedia.org)
The upper panel shows binding of H3K4me3 and Glucocorticoid Receptor around the Zfp866 gene, while the lower panel shows binding of SUZ12 and RING1B across the HoxA gene cluster. (cellsignal.com)
Receptor tyrosine kinases that bind COLLAGENS. (bvsalud.org)

Labib, M., Hedström, M. , Amin, M., Mattiasson, B. A novel competitive capacitive glucose biosensor based on concanavalin A-labeled nano-gold colloids assembled on a polytyramine-modified gold electrode. (lu.se)

1984. Covalent binding of benzidine to rat hemoglobin [Abstract]. (cdc.gov)

Bead- and column-based separation methods rely carefully on the tempo and ease of affinity binding applications. (isotope.info)

When bound to DTC, they make the resulting compound hydrophobic too, creating a shield around it from the surrounding solution. (researchmatters.in)

Initially, the mannose-bound DTC did not show effects of fluorescence when the light was shone on it. (researchmatters.in)

Increases in binding effectiveness with straight chain alcohols was related to chain length. (cdc.gov)
Moreover, PGL-1 seems to be associated with resistance to intracellular killing by macrophages ( 3 ) and promotes phagocytosis of M. leprae by macrophages and Schwann cells by binding to complement component C3 or laminin α2 chain, respectively ( 4 , 5 ). (cdc.gov)

whilst another promising immunogen has included conserved regions III-V of the erythrocyte-binding antigen 175 kDa (PfEBA-175) 10 . (nature.com)

However, when bound with concanavalin, it started emitting fluorescence, thereby signalling its bound state with concanavalin. (researchmatters.in)
Also, this effect increased with increasing concentrations of concanavalin saturating at 309 times the initial intensity of fluorescence. (researchmatters.in)

1. Buffer&Reagent Exchange 를 위해 세포를 Concanavalin A 으로 고정. (seoulin.co.kr)
3. Wash the particles by together with 1mL of Binding Buffer. (isotope.info)
5. Add 0.1mL of Binding Buffer to the serum sample from Step 1. (isotope.info)

MAb 4-13, mAb 4-58 and mAb 5-50 are binding specifically to equine T-cells. (uni-muenchen.de)
Preliminary data suggests that mab 4-39 is binding to a lektin, that can be found mainly on B-Lymphocytes and endothelial cells. (uni-muenchen.de)
23897,8536} At 20 mg/kg concanavalin A is also used to induce liver injury in experimental mouse models of autoimmune hepatitis in order to study immune regulation by macrophages and T cells. (bertin-bioreagent.com)
MATERIALS AND METHODS: We used a Concanavalin A-induced AIH mouse model and lipopolysaccharide-treated Raw264.7 cells to quantify serum biochemical indicators and confirm whether G13 exhibited protective effects in the AIH mice. (bvsalud.org)

Succinyl-Concanavalin A is a high quality Succinyl-Concanavalin A. This product has been used as molecular tool for various biochemical applications. (moleculardepot.com)
Molecular docking simulation revealed that both RDP and TPHP interacted with ER at the binding pocket of estradiol, although the hydrogen bonds were different. (bvsalud.org)
Nixon M. (2017) 'The transient manifold structure of the p53 extreme C-terminal domain: Insight into disorder, recognition, and binding promiscuity by molecular dynamics simulations' . (maynoothuniversity.ie)

Regulation of insulin receptors in frog erythrocytes by insulin and concanavalin A. Evidence for discrete classes of insulin binding sites. (aspetjournals.org)
Unfortunately however, no evidence of anion binding could be obtained for the resulting structures due to both stability problems (sequestering of cations by added anions) and intramolecular hydrogen bonding competing with anion binding. (europa.eu)

23899} Concanavalin A specifically bind. (bertin-bioreagent.com)

In animal models of hepatoma, concanavalin A induces autophagy and cell death and decreases tumor nodule formation. (lktlabs.com)
23900,23898} Concanavalin A is toxic to several tumor cell lines and has been reported to induce programmed cell death of cortical neurons by a mechanism similar to that of the amyloid β peptide. (bertin-bioreagent.com)

10: Mandal DK, Brewer CF. Differences in the binding affinities of dimeric concanavalin A (including acetyl and succinyl derivatives) and tetrameric concanavalin A with large oligomannose-type glycopeptides Biochemistry. (moleculardepot.com)

Capillary electrophoresis analysis of concanavalin A and its succinyl derivative J Pharm Biomed Anal. (moleculardepot.com)

They are characterized by the presence of extracellular DISCOIDIN DOMAINS that activate SIGNAL TRANSDUCTION PATHWAYS in response to collagen binding. (bvsalud.org)

Cross selling for this product : Concanavalin A There are 3 products. (bertin-bioreagent.com)

Effects of hydroxyl compounds on the interaction of concanavalin A with polysaccharides. (cdc.gov)
Effects of bovine serum albumin on the interaction of concanavalin A and succinyl-concanavalin A with phospholipid bilayers Can J Biochem Cell Biol. (moleculardepot.com)
Interaction with concanavalin A and succinyl-concanavalin A Biochim Biophys Acta. (moleculardepot.com)

DTCs are organic compounds whose solubility increases when bound with specific carbohydrates. (researchmatters.in)

They are found throughout nature in both the free and bound form. (lookformedical.com)

The figure shows binding across the HoxD gene cluster, a known target of SUZ12. (cellsignal.com)
The figure shows binding of SUZ12 and JARID2 across the HOXD gene cluster. (cellsignal.com)
Several PRRs have been identified, among them are the TLRs (Toll-like receptors), NLRs (nucleotide-binding and oligomerization domain-like receptors), and RLR (retinoic-acid-inducible gene-1-like receptors) [ 1 ]. (hindawi.com)

It has 4 binding sites, corresponding to the 4 sub-units. (wikipedia.org)

Concanavalin A is used in research to study cell adhesion. (lktlabs.com)
Electrokinetic response of granulocytes to concanavalin A and succinyl-concanavalin A Cell Biophys. (moleculardepot.com)

Mattiasson B. Continuous measurements of a binding reaction using a capacitive biosensor. (lu.se)

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