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  • progenitors
  • Transplantation with autologous hematopoietic progenitors remains an important consolidation treatment for patients with multiple myeloma (MM) and is thought to prolong the disease plateau phase by providing intensive cytoreduction. (edu.au)
  • Another possibility is smoke-related alterations in myeloid or vascular progenitors in the marrow. (arvojournals.org)
  • Concurrently, Cdc42 deficiency caused anemia and splenomegaly accompanied with decreased bone marrow erythroid burst-forming units (BFU-Es) and colony-forming units-erythroid (CFU-Es) activities and reduced immature erythroid progenitors, suggesting that Cdc42 deficiency causes a block in the early stage of erythropoiesis. (bloodjournal.org)
  • The increased myelopoiesis and decreased erythropoiesis of the knockout mice are associated with an altered gene transcription program in hematopoietic progenitors, including up-regulation of promyeloid genes such as PU.1, C/EBP1α, and Gfi-1 in the common myeloid progenitors and granulocyte-macrophage progenitors and down-regulation of proerythroid gene such as GATA-2 in the megakaryocyte-erythroid progenitors. (bloodjournal.org)
  • vivo
  • Two weeks after in vivo transfer of MSC, we detected significantly reduced serum levels of anti ds-DNA antibodies and 24 hour proteinuria in MRL/lpr mice as compared with control groups without MSC transplantation. (gulfcoaststemcell.com)
  • More specifically, we established in vivo differentiation system generating fully functional HSCs from mouse induced pluripotent stem cells. (nii.ac.jp)
  • apoE
  • Inactivation of the apoE gene in mice leads to a prominent increase in serum cholesterol and triglyceride levels and the development of premature atherosclerosis. (ahajournals.org)
  • The concentration of apoE detected in serum was found to be gene dosage dependent, being 3.52±0.30%, 1.87±0.17%, and 0% of normal in transplanted mice receiving either apoE +/+ , apoE +/− , or apoE −/− bone marrow, respectively. (ahajournals.org)
  • After 4 months on a "Western-type" diet, atherosclerosis was evidently reduced in mice transplanted with apoE +/+ bone marrow, compared with control transplanted mice. (ahajournals.org)
  • The serum half-life of (β)VLDL in transplanted mice, compared with control apoE-deficient mice, was shortened mainly as a consequence of an increased recognition and uptake by the liver. (ahajournals.org)
  • In conclusion, macrophage-derived apoE can dose-dependently reduce hypercholesterolemia in apoE-deficient mice owing to increased recognition and uptake of (β)VLDL by parenchymal liver cells, leading to a decreased susceptibility to atherosclerosis. (ahajournals.org)
  • Recently, apoE-deficient mice have been generated by targeted inactivation of the apoE gene in embryonic stem cells. (ahajournals.org)
  • 11-13 Inactivation of the apoE gene in these mice is associated with a prominent increase in serum cholesterol levels and the development of premature atherosclerosis. (ahajournals.org)
  • Methods and Results- Apolipoprotein E (apoE)-knockout (KO) mice were divided into 4 groups: 20 nonischemic mice receiving intravenous injection of either saline (n=10) or 10 6 BM-MNCs from wild-type animals (n=10) and 20 mice with arterial femoral ligature receiving intravenous injection of either saline (n=10) or 10 6 BM-MNCs from wild-type animals (n=10) at the time of ischemia induction. (ahajournals.org)
  • However, transplantation of 10 6 BM-MNCs into apoE-KO mice with hindlimb ischemia induced a significant 48% to 72% increase in lesion size compared with the other 3 groups ( P =0.0025), despite similar total cholesterol levels. (ahajournals.org)
  • Transplantation of 10 5 BM-MNCs produced similar results, whereas transplantation of 10 6 apoE-KO-derived BM-MNCs had neither proangiogenic nor proatherogenic effects. (ahajournals.org)
  • In the present study, we examined the effects of transplantation of BM-MNCs on the extent and composition of atherosclerotic plaques in the aortic sinus of apolipoprotein E (apoE)-knockout (KO) mice. (ahajournals.org)
  • Bone marrow cells were obtained by flushing the tibias and femurs of male 100% C57BL/6J wild-type or apoE-KO mice. (ahajournals.org)
  • Objective- We first showed that absence of p53 accelerates atherosclerosis development in apoE-deficient mice. (ahajournals.org)
  • Our laboratory first reported the effect of p53 inactivation on atherosclerosis development in apoE-deficient mice. (ahajournals.org)
  • 7 We found that, despite a similar degree of hypercholesterolemia, p53 −/− /apoE −/− mice developed aortic atherosclerosis much faster than p53 +/+ /apoE −/− littermates. (ahajournals.org)
  • Although this result corroborated that of Guevara et al 7 on apoE −/− mice, there was apparently a difference in the mechanism that led to the increase in lesion size in the two mouse models. (ahajournals.org)
  • In contradiction to the observation of Guevara et al 7 on p53 −/− /apoE −/− mice, van Vlijmen et al 8 found an insignificant trend toward increased apoptosis but no difference in cellular proliferation as measured by BrdU labeling in the apoE*3-Leiden mice that received p53 −/− marrow. (ahajournals.org)
  • The two mutant apoE mouse models develop accelerated atherosclerosis because of the massive accumulation of remnant lipoproteins, a highly unusual cause of atherosclerosis in humans. (ahajournals.org)
  • ischemic
  • 2 In particular, transplantation of bone marrow-derived mononuclear cells (BM-MNCs) has been shown to stimulate neovascularization after experimental ischemic injury, resulting in long-term salvage and survival of viable tissue, reduced tissue remodeling, and improved organ function. (ahajournals.org)
  • To address this issue, BMSCs were implanted into the ischemic barrel cortex of adult mice 1 and 7 days after focal barrel cortex stroke. (drcalapai.net)
  • The recovered circuit activity likely contributes to the improved sensorimotor function after focal ischemic stroke and BMSC transplantation. (drcalapai.net)
  • serum
  • Removing mature T cells from the marrow with anti-Thy 1.2 serum and complement before injection prevented GVHD. (rupress.org)
  • Monaco AP, Wood ML and Russel PS (1966) Studies on heterologous anti-lymphocyte serum in mice: III. (springer.com)
  • 1976) Possible active enhancement of a human cadaver renal allograft with antilymphocytic serum (ALS) and donor bone marrow: Case report of an initial attempt. (springer.com)
  • phenotype
  • Conclusions- p53 plays a crucial role in atherosclerosis lesion development and remodeling, and macrophage-specific p53 deficiency stimulates cellular proliferation leading to a vulnerable-appearing phenotype of lesions in a mouse model of familial hypercholesterolemia. (ahajournals.org)
  • recipient mice
  • Hill and colleagues have first highlighted the role of conditioning regimen in LPS leakage in the blood circulation and demonstrated that higher dose of irradiation in recipient mice correlated with more lethal GVHD. (frontiersin.org)
  • atherosclerosis
  • In this study, we investigated how macrophage-specific loss of p53 function might modulate atherosclerosis development in LDL receptor-deficient mice, a model for familial hypercholesterolemia. (ahajournals.org)
  • survival
  • This work demonstrates that stem cell transplantation in the muscle prolonged the lifespan, increased motoneuron survival and slowed disease progression, which was also assessed by genetic expression analysis. (biomedcentral.com)
  • BMSC transplantation also promoted directional migration and survival of doublecortin (DCX)-positive neuroblasts in the peri-infarct region. (drcalapai.net)
  • 1986) Prolongation of renal allograft survival in antilymphocyte-serumtreated dogs by postoperative injection of density-gradientfractionated donor bone marrow. (springer.com)
  • significantly
  • Two days after the first transplantation (3 days after stroke), the infarct volume determined by TTC staining was significantly smaller in BMSC-treated compared to vehicle-treated stroke mice. (drcalapai.net)
  • Immunohistochemical staining disclosed that the density of neurons, axons, and blood vessels in the peri-infarct region was significantly higher in BMSC-treated mice, accompanied with enhanced local blood flow recovery. (drcalapai.net)
  • Histopathological examination showed significantly reduced renal pathology in MSC-treated mice. (gulfcoaststemcell.com)
  • Taken together, we have demonstrated that transplantation of human MSC can significantly inhibit the autoimmune progression in MRL/lpr mice. (gulfcoaststemcell.com)
  • He has coauthored more than 70 original papers or chapters and has contributed significantly to both the pediatric surgical and the transplantation literature. (childrenshospital.org)
  • outcomes
  • BMT with and without irradiation yielded no difference in BV and BMD outcomes for both OI and WT mice, at 3 weeks. (springer.com)
  • Ramos-Gonzalez G, LaQuaglia M, O'Neill AF, Elisofon S, Zurakowski D, Kim HB, Vakili K. Long-term outcomes of liver transplantation for hepatoblastoma: A single-center 14-year experience. (childrenshospital.org)
  • novel
  • The present investigation thus provides novel evidence that BMSC transplantation has the potential to repair the ischemia-damaged neural networks and restore lost neuronal connections. (drcalapai.net)
  • normal
  • Mice exposed to cigarette smoke demonstrated typically large CNV compared to normal controls without smoke exposure. (arvojournals.org)
  • In contrast, in the inverse experiment, mice that received four weeks of smoke exposure prior to BMT, then reconstituted with normal marrow, demonstrated no difference in size of CNV from control mice (n=6, 2.5±0.3 DA for normal receiving normal marrow v. 2.6±0.5 DA for smoked mice receiving normal marrow, p=ns). (arvojournals.org)
  • myeloid
  • We report here that inducible deletion of cdc42 in cdc42 -floxed mouse bone marrow by the interferon-responsive, Mx1-Cre -mediated excision led to myeloid and erythroid developmental defects. (bloodjournal.org)
  • Cdc42-deficient mice developed a fatal myeloproliferative disorder manifested by significant leukocytosis with neutrophilia, myeloid hyperproliferation, and myeloid cell infiltration into distal organs. (bloodjournal.org)
  • models
  • Many approaches which have proven successful in rodent models of solid organ or cellular transplantation have not translated well to large animals or humans. (springer.com)
  • cell
  • and/or the alternative, smoke alters vascular precursor biology, such that the effect of smoke resides in the vascular precursor cell subset in the marrow. (arvojournals.org)