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  • assays
  • Flow cytometry, expansion cultures, long-term BM cultures and clonogenic progenitor cell assays were used. (gulfcoaststemcell.com)
  • BrdU labeling of proliferative cells, biochemical assays of serum, cerebrospinal fluid and brain levels of trophic factors, growth factors, and stress-related proteins are proposed as indices of neurogenesis, while quantitative measurements of spontaneous movements will reveal psychomotor components of immobilization. (cognizantcommunication.com)
  • As ML-ICs and SRCs were both detected in blood of AC-SCD patients only, these assays may both measure primitive progenitors. (jci.org)
  • Stem cells can also be identified by functional assays. (wikipedia.org)
  • Below is a list of genes/protein products that can be used to identify various types of stem cells, or functional assays that do the same. (wikipedia.org)
  • These properties can be illustrated with relative ease in vitro, using methods such as clonogenic assays, where the progeny of a single cell is characterized. (wikipedia.org)
  • vascular
  • Stromal vascular fraction derived stem cells are not a recognized therapy for any known disease or disorder. (gulfcoaststemcell.com)
  • Hematopoietic stem cell transplantation rescues the hematological, immunological and vascular phenotype in DADA2. (amedeo.com)
  • The A2b adenosine receptor (A2bAR) is highly abundant in bone marrow macrophages and vascular smooth muscle cells (VSMC). (pnas.org)
  • Our earlier studies demonstrated that the A2bARs are notably expressed in vascular cells and macrophages and protect against excessive vascular adhesion ( 10 ), suggesting that A2bARs mediate a protective effect on the vasculature. (pnas.org)
  • In the current study we examined the role of vascular and bone marrow adenosine receptors in vascular response to injury using a model that mimics human restenosis after angioplasty. (pnas.org)
  • The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. (wikipedia.org)
  • Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
  • Injection of CD34+ hematopoietic Stem Cells has been clinically applied to treat various diseases including Spinal Cord Injury, Liver Cirrhosis and Peripheral Vascular disease. (wikipedia.org)
  • Flow cytometric quantification can be used to purify adipocytes from the stromal vascular fraction of most fat depots. (wikipedia.org)
  • mice
  • To test whether there may be an advantage of one approach over the other for WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome - a primary immunodeficiency disorder caused by gain-of-function autosomal dominant mutations in chemokine receptor CXCR4 - we performed competitive transplantation experiments using both lethally irradiated WT (Cxcr4+/+) and unconditioned WHIM (Cxcr4+/w) recipient mice. (jci.org)
  • Bone marrow and HSC from green fluorescent protein (GFP) transgenic mice were transplanted into irradiated wild‐type mice. (pubmedcentralcanada.ca)
  • At 1 month after transplantation, mice were sacrificed and their sclera examined by histology, immunohistochemistry (CD11b, CD11c, CD45), and transmission and scanning electron microscopy. (pubmedcentralcanada.ca)
  • To investigate bone marrow derived cell recruitment under inflammatory conditions, experimental autoimmune uveitis (EAU) was induced in transplanted mice. (pubmedcentralcanada.ca)
  • In EAU mice, vast infiltration of GFP (+) cells developed into the sclera. (pubmedcentralcanada.ca)
  • An etiological connection between BCR / ABL and leukemia is indicated by the observation that transgenic mice bearing a BCR/ABL DNA construct develop leukemia of B, T, and myeloid cell origin. (springer.com)
  • Her group is using cells and serum from both mice and primary specimens from healthy and diseased patients for these studies and using a variety of exciting new tools and methods to unlock future discoveries. (stanford.edu)
  • Mutant calreticulin knock-in mice develop thrombocytosis and myelofibrosis without a stem cell self-renewal advantage. (amedeo.com)
  • Hematopoietic stem cell gene therapy for IFNgammaR1 deficiency protects mice from mycobacterial infections. (amedeo.com)
  • Physiological Srsf2 P95H expression causes impaired hematopoietic stem cell functions and aberrant RNA splicing in mice. (amedeo.com)
  • In accordance, CXCR4, which is known to attract progenitor cells during tissue regeneration, is up-regulated in lesions of the KO mice. (pnas.org)
  • Bone marrow transplantation experiments indicated that the majority of the signal for lesion formation in the null mice originates from bone marrow cells. (pnas.org)
  • It is important to mention that Long-Term Hematopoietic Stem Cells (LT-HSCs) in mice and humans are the hematopoietic cells with the greatest self-renewal capacity. (wikipedia.org)
  • citation needed] Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. (wikipedia.org)
  • Knockout mice which genetically lack IL-7 receptor exhibit thymic atrophy, arrest of T-cell development at the double positive stage, and severe lymphopenia. (wikipedia.org)
  • Administration of IL-7 to mice results in an increase in recent thymic emigrants, increases in B and T cells, and increased recovery of T cells after cyclophosphamide administration or after bone marrow transplantation. (wikipedia.org)
  • By continuous intravital imaging in mice, researchers were able to explore the structure of the stem cell niche and to obtain the fate of individual stem cells (SCs) and their progeny over time in vivo. (wikipedia.org)
  • The gene encoding stem cell factor (SCF) is found on the Sl locus in mice and on chromosome 12q22-12q24 in humans. (wikipedia.org)
  • Mice with SCF or c-Kit mutations have severe defects in the production of mast cells, having less than 1% of the normal levels of mast cells. (wikipedia.org)
  • A study in mice has shown that G-CSF may decrease bone mineral density. (wikipedia.org)
  • Balanced (Bala) HSCs repopulate peripheral white blood cells in the same ratio of myeloid to lymphoid cells as seen in unmanipulated mice (on average about 15% myeloid and 85% lymphoid cells, or 3 ≤ ρ ≤ 10). (wikipedia.org)
  • umbilical
  • In another study, myeloablative therapy followed by infusion of unrelated umbilical cord blood cells was performed in 57 adult patients with high-risk, hematological disease. (biomedcentral.com)
  • The FDA has approved five hematopoietic stem-cell products derived from umbilical cord blood, for the treatment of blood and immunological diseases. (wikipedia.org)
  • The term encompasses multipotent cells derived from other non-marrow tissues, such as placenta, umbilical cord blood, adipose tissue, adult muscle, corneal stroma or the dental pulp of deciduous baby teeth. (wikipedia.org)
  • self-renewal
  • A niche is a subgroup of tissue cells and extracellular substrates that can indefinitely harbor one or more stem cells and control their self-renewal and progeny in vivo [ 3 ]. (hindawi.com)
  • A stem cell possesses two properties: Self-renewal, which is the ability to go through numerous cycles of cell division while still maintaining its undifferentiated state. (wikipedia.org)
  • Symmetric division gives rise to two identical daughter cells, both endowed with stem cell properties, whereas asymmetric division produces only one stem cell and a progenitor cell with limited self-renewal potential. (wikipedia.org)
  • Stem cell self-renewal is thought to occur in the stem cell niche in the bone marrow, and it is reasonable to assume that key signals present in this niche will be important in self-renewal. (wikipedia.org)
  • epithelial
  • This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. (wikipedia.org)
  • Differentiated airway epithelial cells can revert into stable and functional stem cells in vivo. (wikipedia.org)
  • Stromal cells include epithelial cells of the thymic cortex and medulla, and dendritic cells. (wikipedia.org)
  • The cortex is mainly made up of thymocytes, supported by a network of finely-branched epithelial reticular cells, which is continuous with a similar network in the medulla. (wikipedia.org)
  • These are concentric, layered whorls of epithelial cells that increase in number throughout life. (wikipedia.org)
  • extracellular
  • It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • Several factors are important to regulate stem-cell characteristics within the niche: cell-cell interactions between stem cells, as well as interactions between stem cells and neighbouring differentiated cells, interactions between stem cells and adhesion molecules, extracellular matrix components, the oxygen tension, growth factors, cytokines, and the physicochemical nature of the environment including the pH, ionic strength (e.g. (wikipedia.org)
  • The cells, which are long and thin, are widely dispersed and the adjacent extracellular matrix is populated by a few reticular fibrils but is devoid of the other types of collagen fibrils. (wikipedia.org)
  • It is a highly complex process involving extensive interplay between cells, soluble factors, and the extracellular matrix (ECM). (wikipedia.org)
  • dendritic
  • We have provided direct and novel evidence for the migration of bone marrow and HSC cells into the sclera differentiating into macrophages and dendritic cells. (pubmedcentralcanada.ca)
  • Macrophages and dendritic cells are bone marrow derived antigen presenting cells (APC) which serve in the first step of the acquired immune responses. (pubmedcentralcanada.ca)
  • Recently, the presence of APCs such as macrophages and dendritic cells was reported in the cornea. (pubmedcentralcanada.ca)
  • 15 , 16 The CD45+/CD11b+/CD11c+ dendritic cells distributed in corneal epithelium 8 and corneal stroma, 9 , 17 and CD11b+/CD11c‐ macrophages in the corneal stroma. (pubmedcentralcanada.ca)
  • Cox regression analyses evaluated risk strata, age, gender, and the numbers of nucleated cells, CD3-positive T cells, CD34-positive hematopoietic cells, and type 2 dendritic cells (DC2) as covariates for event-free survival, relapse, and nonrelapse mortality. (cancernetwork.com)
  • Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. (wikipedia.org)
  • murine
  • Here, we show that bone marrow stromal cells express endocannabinoids (anandamide and 2-arachidonylglycerol), whereas CB₂ receptors are expressed in human and murine HSPCs. (420magazine.com)
  • citation needed] CD34 is expressed in roughly 20% of murine hematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • Working under the supervision of Bruce (AACR Award), and in association with Till and McCulloch and a vibrant group of graduate students and post-doctoral fellows, Eaves completed his PhD in Medical Biophysics at the University of Toronto with a thesis entitled Studies on the Control of Murine Bone Marrow Function (1974). (wikipedia.org)
  • endogenous
  • In another study, upon exposure to cyclosporine A, an immunosuppressant, there was enhanced recovery of cortical injury following stroke secondary to endogenous stem cell activity and migration [ 2 ]. (mdpi.com)
  • Pharmacological activation of endogenous neural stem cells has been reported to induce neuroprotection and behavioral recovery in adult rat models of neurological disorder. (wikipedia.org)
  • maturation
  • Certain cytokines such as IL-3, IL-6, IL-11, LIF, erythropoietin, and thrombopoietin all stimulate the maturation of megakaryocytic progenitor cells. (wikipedia.org)
  • One example is the transformation of iris cells to lens cells in the process of maturation and transformation of retinal pigment epithelium cells into the neural retina during regeneration in adult newt eyes. (wikipedia.org)
  • fetal
  • In fact, fetal HSCs in cell culture are 6 times more sensitive to SCF than adult HSCs based on the concentration that allows maximum survival. (wikipedia.org)
  • In mammalian embryology, the first definitive HSCs are detected in the AGM (aorta-gonad-mesonephros), and then massively expanded in the fetal liver prior to colonising the bone marrow before birth. (wikipedia.org)
  • allogenic stem
  • As one may expect, the use of allogenic stem cells may predispose an individual to various immunologic complications upon treatment, giving rise to the significant limitation of graft rejection with this method of treatment. (mdpi.com)