• The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. (wikipedia.org)
  • The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
  • Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
  • Endothelial progenitor cells (EPCs) contribute to neovascularization and play an important role in the development of these diseases. (hindawi.com)
  • The bone-marrow-derived endothelial progenitor cells (EPCs) provide an alternative source of endothelial cells (ECs) that contributes to neovessel formation in endothelium structure [ 4 , 5 ]. (hindawi.com)
  • The bone morrow contains several stem cell types including hematopoetic stem cells, endothelial progenitor cells, mesenchymal (stromal) stem cells and multipotent adult progenitor cells. (escardio.org)
  • Although there is no definitive evidence, combinations of progenitor cells seem to be more benefical than specific stem cell type for cardiac repair (3). (escardio.org)
  • Interestingly, contradictory data exist for transformation of bone marrow derived progenitor cells (BMPC) into new cardiomyocytes and alternative mechanisms as enhanced neovascularization, enhanced scar tissue formation due to augmented inflammatory response and decreased apoptosis have been suggested for the benefical effects of these cells on myocardial function after myocardial infarction (4). (escardio.org)
  • Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
  • Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
  • Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • Progenitor cell therapy describes the use of multipotent cells of various cell lineages (autologous or allogeneic) for tissue repair and/or regeneration. (southcarolinablues.com)
  • Progenitor cell therapy is being investigated for the treatment of damaged myocardium resulting from acute or chronic cardiac ischemia and for refractory angina. (southcarolinablues.com)
  • For individuals who have acute cardiac ischemia who receive progenitor cell therapy, the evidence includes 2 randomized controlled trials (RCTs) with 200 patients, numerous small RCTs, and meta-analyses of these RCTs. (southcarolinablues.com)
  • Overall, this evidence has suggested that progenitor cell treatment may be a promising intervention, but robust data on clinical outcomes are lacking. (southcarolinablues.com)
  • For individuals who have chronic cardiac ischemia who receive progenitor cell therapy, the evidence includes a nonrandomized comparative trial and systematic reviews of smaller RCTs. (southcarolinablues.com)
  • Results of the nonrandomized trial are encouraging, because this is the first controlled trial that has reported a significant mortality benefit for progenitor cell treatment. (southcarolinablues.com)
  • For individuals who have refractory angina who receive progenitor cell therapy, the evidence includes phase 2 trials and a phase 3 pivotal trial. (southcarolinablues.com)
  • Additional larger trials are needed to determine whether progenitor cell therapy improves health outcomes in patients with refractory angina. (southcarolinablues.com)
  • 1 Treatment with progenitor cells (i.e., stem cells) offers potential benefits beyond those of standard medical care, including the potential for repair and/or regeneration of damaged myocardium. (southcarolinablues.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • Enhancement of human erythroid progenitor cell growth by media conditioned by a human t-lymphocyte line. (shengsci.com)
  • Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
  • We investigated the origin of SF3B1 mutations within the bone marrow hematopoietic stem and progenitor cell compartments in patients with MDS-RS. (nih.gov)
  • All recurrent mutations identified in mononuclear cells could be tracked back to the phenotypically defined hematopoietic stem cell (HSC) compartment in all investigated patients and were also present in downstream myeloid and erythroid progenitor cells. (nih.gov)
  • While in agreement with previous studies, little or no evidence for clonal ( SF3B1 mutation) involvement could be found in mature B cells, consistent involvement at the pro-B-cell progenitor stage was established, providing definitive evidence for SF3B1 mutations targeting lymphomyeloid HSCs and compatible with mutated SF3B1 negatively affecting lymphoid development. (nih.gov)
  • Assessment of stem cell function in vitro as well as in vivo established that only HSCs and not investigated progenitor populations could propagate the SF3B1 mutated clone. (nih.gov)
  • In preclinical and clinical studies, progenitor cell therapy (cord blood and mesenchymal stem cells) has shown promise in reversing the underlying pathology of SNHL, the loss of cochlear sensory hair cells. (intechopen.com)
  • Progenitor cell therapy may also allow functional reorganization of the auditory pathways including primary auditory cortex (Heschl's gyrus). (intechopen.com)
  • We will present a summary of the effect of hearing loss on auditory development, existing preclinical and clinical data on progenitor cell therapy, and its potential role in the (re)habilitation of non-genetic SNHL. (intechopen.com)
  • Since then, allogeneic hematopoietic stem cell transplantation (HSCT) has evolved to become a frequently used and effective therapy for many hematologic malignancies. (longdom.org)
  • His research focus is on haematopoietic stem cell transplantation (HSCT) and translational research of normal and malignant stem cells. (edu.au)
  • It may also mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (issca.us)
  • Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (issca.us)
  • This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (issca.us)
  • Marrow cells enriched greater than or equal to 500-fold for LTC-IC (1-2% pure) by flow cytometry showed gene transfer efficiencies of 27% when infected in a similar fashion over a shorter period (24 h), but in the presence of added soluble IL-3 and G-CSF without stromal feeders, and this increased to 61% when Steel factor was also present during the infection period. (jci.org)
  • This paper describes successful treatment in three patients with Achilles tendonosis and interstitial tears using with non-manipulated autologous adipose derived stem/stromal cells and high-density platelet rich plasma concentrates (or bone marrow/platelet concentrate combination). (journalofprolotherapy.com)
  • Introduce a safe, practical, and effective protocol for minimally invasive treatment of Achilles tendon tears using non-manipulated autologous adipose-derived tissue stromal vascular fraction with additive of high-density platelet-rich plasma or bone marrow concentrates. (journalofprolotherapy.com)
  • Defining essential stem cell characteristics in adipose-derived stromal cells extracted from distinct anatomical sites. (ui.ac.id)
  • Peripheral blood mononuclear cells were obtained after admission by leukapheresis. (cellsurgicalnetwork.com)
  • Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque gradient from the blood samples collected at days 0, 7, 14, 28, and final day of transplantation. (magiran.com)
  • Bone marrow mononuclear cells (BMMNCs) consist of hemopoietic stem cells and mesenchymal stem cells together with progenitors of endothelial cells or somatic cells [ 1 , 2 ]. (biomedcentral.com)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • The EA.hy926 cell line was derived from human umbilical vein endothelial cells, and it has been shown to express differentiated functions that are characteristics of human vascular endothelium [66, 67]. (phosphatases.com)
  • However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones (B lymphocyte precursors) and CD34+ megakaryocytes. (wikipedia.org)
  • Despite reinduction therapy and 2 blocks of high-intensity chemotherapy, he had refractory disease that manifested as persistent cytopenia with more than 80% blasts in bone marrow, all of which were expressing CD7. (ajmc.com)
  • Preclinical and some small-scaled clinical trials have suggested feasibility and safety of cardiac stem cell therapy. (escardio.org)
  • Cell therapy is currently emerging as a potential new treatment for post MI patients with the assumption that recolonization of the areas of scarred myocardium with exogenously supplied surrogates or precursors of cardiomyocytes can restore function and ultimately affect clinical outcomes. (escardio.org)
  • Various cell types have been tested experimentally for cardiac repair so far, but only those of autologous origin have yet undergone clinical testing due to immune competency. (escardio.org)
  • In experimental and clinical studies unselected mononuclear bone marrow cells as well as specific subpopulations have been used for transplantation. (escardio.org)
  • It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
  • We also process hematopoietic stem cell, bone marrow, and mononuclear cell collections for hematopoietic stem cell transplantation as well as treatment of a wide variety of other clinical disorders. (uiowa.edu)
  • Engraftment, clinical, and molecular follow-up of patients with multiple myeloma who were reinfused with highly purified CD34+ cells to support single or tandem high-dose chemotherapy. (shengsci.com)
  • Eighty-two patients with advanced multiple myeloma (MM) were enrolled in 2 sequential clinical studies of 1 or 2 courses of myeloablative therapy with stem cell support. (shengsci.com)
  • Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
  • Moreover, xenotransplantation accurately predicted results in canine-to-canine allogeneic transplantation experiments, an important pre-clinical model for future human clinical trials. (biomedcentral.com)
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
  • Therefore, a central goal in clinical care of SDS patients is to identify incipient leukemic transformation and initiate pre-emptive treatment with allogeneic stem cell transplantation. (nature.com)
  • More recently, few clinical attempts have offered encouraging results with ex vivo or stem cell-based engineered airways and tracheal allografts implanted after heterotopic revascularization. (biomedcentral.com)
  • Clinical uses of gene transfer to bone marrow transplants require the establishment of a reproducible method for infecting large numbers of very primitive hematopoietic cells at high efficiency using cell-free retrovirus-containing media. (jci.org)
  • By using a less highly enriched population of LTC-IC obtained by a bulk immunoselection technique applicable to large-scale clinical marrow harvests, gene transfer efficiencies to LTC-IC of 40% were achieved and this was increased to 60% by short-term preselection in G418. (jci.org)
  • These data demonstrate the feasibility of using cell-free virus preparations for infecting clinical marrow samples suitable for transplantation, as well as for further analysis of human marrow stem cell dynamics in vitro. (jci.org)
  • Mesenchymal stem cells: clinical applications and biological characterization. (ui.ac.id)
  • Safety of mesenchymal stem cells for clinical application. (ui.ac.id)
  • Ma DD , 2007 , 'Expression of Neurofilament Proteins in Adult Bone Marrow Mesenchymal Stem Cells and Embryonic Stem Cells and Their Clinical Potential' , in Davenport LP (ed. (edu.au)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • Initial management consisted of en una paciente con topical corticosteroids and oral antihistamines with little clinical response. (bvsalud.org)
  • CD34 is expressed in roughly 20% of murine haematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • 2 Haematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. (nih.gov)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • however, their role in β-cell regeneration remains elusive. (drcalapai.com)
  • The idea of myocardial regeneration with stem cell transplantation after myocardial infarction receives tremendous interest. (escardio.org)
  • BACKGROUND: Bone marrow stem cell clonal dysfunction by somatic mutation is suspected to affect post-infarction myocardial regeneration after coronary bypass surgery (CABG). (bvsalud.org)
  • Portal application of autologous CD133+ bone marrow cells to the liver: a novel concept to support hepatic regeneration. (e3ligase-signal.com)
  • 7 Yamada Y, Yokoyama S Fukuda N, et al A novel approach for myocardial regeneration with educated cord blood cells cocultured with cells from brown adipose tissue J . Biochem Biophys Res Commun 2007 353(1) 182-188. (xml-journal.net)
  • For example, embryonic stem cells exist only at the earliest stage of embryo and adult stem cells appear during fetal development and are retained throughout life. (bmrat.org)
  • Embryonic stem cells were first identified in mice (Martin, 1981). (bmrat.org)
  • Embryonic stem cells are pluripotent, meaning they are able to produce all cell types in the body. (bmrat.org)
  • These cells exist only in earliest stages of embryonic development known as the blastocyst stage. (bmrat.org)
  • Recently, scientists have discovered how to reprogram normal cells to behave like embryonic stem cells. (bmrat.org)
  • This is done by re-activating critical genes that define embryonic stem cells to make adult stem cells to revert to an embryonic-like state of pluripotency. (bmrat.org)
  • An early boost of intracardiac regenerative key mechanisms and angiogenetic niche signaling in cardiac mesenchymal stem cells (MSCs) could improve myocardial infarction (MI) healing. (bvsalud.org)
  • Previous studies from our group have shown that autologous adipose-derived regenerative cells (ADRCs) represent a promising approach to improve wound healing and, thereby, impact HTS development. (biomedcentral.com)
  • After more than 50 years of research, airway transplantation remains a major challenge in the fields of thoracic surgery and regenerative medicine. (biomedcentral.com)
  • Cell-based therapy has expanded its influence in cancer immunotherapy, regenerative medicine, and tissue engineering. (biomedcentral.com)
  • In particular, stem cells, including mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs), and induced pluripotent stem cells (iPSCs), and immune cells, such as T-cells and Natural Killer (NK) cells, have been favored candidates for regenerative medicine and cell-based cancer immunotherapy, respectively. (biomedcentral.com)
  • Unfortunately, these breakthrough discoveries in both regenerative medicine and cancer immunotherapy using cells as therapeutic reagents soon faced a common problem: the inability to control cellular functions to maximize the therapeutic benefits. (biomedcentral.com)
  • The developmental story of cell-based cancer immunotherapy is not so different from MSCs in regenerative medicine. (biomedcentral.com)
  • Dr. Novas holds key positions in prestigious organizations related to regenerative medicine and stem cell research, including the Gene-Therapy Biomedical Research College in Wellington, Florida, and the Far Eastern University in Manila, Philippines. (regenestem.com)
  • Stem cells are not only widely used for regenerative medicine, but are also considered as a useful tool for cancer treatment. (bmrat.org)
  • Prospect of adipose tissue derived mesenchymal stem cells in regenerative medicine. (ui.ac.id)
  • Suspension culture of human mast cells/basophils from umbilical cord blood mononuclear cells. (virascience.com)
  • Objective(s) Human umbilical cord blood (HUCB) is now considered as a valuable source for stem cell-based therapies. (virascience.com)
  • Human cord blood as a potential source of stem cells J . Curr Vasc Pharmacol 2007 5(1) 27-44. (xml-journal.net)
  • 3 Hirata Y Sata M Motomura N, et al Human umbilical cord blood cells improve cardiac function after myocardial infarction J . Biochem Biophys Res Commun 2005 327(2) 609-614. (xml-journal.net)
  • 12 Henning RJ Abu-Ali H Balis JU, et al Human umbilical cord blood mononuclear cells for the treatment of acute myocardial infarction J . Cell Transplant 2004 13(7-8) 729-739. (xml-journal.net)
  • Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
  • The recent emergence of cell-based therapies such as autologous bone marrow-derived mesenchymal stem cells (ABM-MSCs) and mononuclear cells (ABM-MNCs) seems to offer a pragmatic approach to augment β-cell function/mass. (drcalapai.com)
  • Improvement in insulin sensitivity with MSCs and increase in C-peptide response with MNCs provide newer insights in cell-based therapies. (drcalapai.com)
  • Concomitant fluorescence-activated cell sorting, co-cultures with neonatal cardiomyocytes, angiogenesis assays, ELISA, western blotting and RAMAN spectroscopy demonstrated that EPO could promote cardiomyogenic differentiation that was specific of tissue origin and enhance paracrine angiogenetic activity in cardiac CD45-CD44+DDR2+ MSCs. (bvsalud.org)
  • The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. (springer.com)
  • MSCs were obtained from the bone marrow (BM) cells remaining in the bag and filter used in the transplantation of hematopoietic stem cells. (springer.com)
  • NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. (springer.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (issca.us)
  • Therefore, the authors concluded that transplantation of isolated healthy allogeneic MSCs might cure the disease. (issca.us)
  • This implies homing of transplanted MSCs to sites in bone marrow and/or bone. (issca.us)
  • In a second study [6], these authors showed that autologous, enzyme-deficient MSCs transduced with a copy of the intact gene resulted in normal collagen production in bone cavities. (issca.us)
  • The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (issca.us)
  • and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (issca.us)
  • Adipose tissue derived mesenchymal stem cells (AT-MSCs) are relatively easy in isolation procedure compared to bone marrow-derived. (ui.ac.id)
  • This experimental study used cryopreserved adipose tissue derived MSCs stored in Stem Cell Medical Technology Integrated Service Unit Cipto Mangunkusumo Hospital. (ui.ac.id)
  • Cell therapy is a potential new treatment for post MI patients, but the main challenges are : the necessity to preserve immune competency and to gain adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
  • As such, stem cell therapy cannot be regarded as a valid therapeutic option for patients with cardiovascular disease in the present era of evidenced-based medicine, yet it holds great hope for the future. (escardio.org)
  • Several preliminary reports have demonstrated that local stem cell transplantation in patients with acute myocardial infarction is safe and may lead to improved myocardial function and perfusion. (escardio.org)
  • Stem-cell transplantation into the frontal motor cortex in amyotrophic lateral sclerosis patients. (cellsurgicalnetwork.com)
  • Ten patients with confirmed ALS without transplantation were used as a control group. (cellsurgicalnetwork.com)
  • RESULTS: The autologous transplantation of CD133(+) stem cells into the frontal motor cortex is a safe and well-tolerated procedure in ALS patients. (cellsurgicalnetwork.com)
  • This study aimed to identify the density of mononuclear cells (MNCs) and CD34+ cells in the bone marrow of patients with three neurologic conditions. (biomedcentral.com)
  • Bone marrow aspiration was conducted from the patients' bilateral anterior iliac crest under general anesthesia in an operating theater. (biomedcentral.com)
  • The density of MNCs was 5.22 million cells per mL and 5.03% CD34+ cells in patients with three neurologic conditions. (biomedcentral.com)
  • The highest density of MNCs in each ml of bone marrow was found in patients with CP due to OD, whereas the percentage of CD34+ cells was the highest among patients with CP related to NI. (biomedcentral.com)
  • In this study, we aimed to identify the concentrations of mononuclear cells (MNCs) and CD34+ cells in the bone marrow of these patients and their correlation with treatment outcomes. (biomedcentral.com)
  • Bone marrow aspiration was conducted from the patients' bilateral anterior iliac crest under general anesthesia in an operating theater at Vinmec Times City International Hospital. (biomedcentral.com)
  • New research in clustered regularly interspaced short palindromic repeats (CRISPR) technology suggests that base-edited T cells could be useful in patients with relapsed leukemia and could help anticipate the risks of immunotherapy -related complications. (ajmc.com)
  • Most children with T-cell acute lymphoblastic leukemia (ALL) can be treated with standard chemotherapy regimens, but patients with induction failure or elevated minimal residual disease after consolidation generally receive allogeneic stem-cell transplantation. (ajmc.com)
  • Patients who relapse after transplantation have a poor prognosis, with less than 15% long-term survival. (ajmc.com)
  • For these patients, cellular immunotherapy could help achieve deep leukemic clearance, although chimeric antigen receptor (CAR) T-cell strategies against T-cell cancers have been difficult. (ajmc.com)
  • We also collect hematopoietic stem cells and mononuclear cells from healthy donors and patients. (uiowa.edu)
  • Autologous infusion of expanded mobilized adult bone marrow-derived CD34+ cells into patients with alcoholic liver cirrhosis. (e3ligase-signal.com)
  • Feasibility and safety of autologous bone marrow mononuclear cell transplantation in patients with advanced chronic liver disease. (e3ligase-signal.com)
  • Between February 1986 and March 1990, 56 patients with relapsed Hodgkin's disease treated with high-dose cyclophosphamide, carmustine, and etoposide (CBV) received an autologous peripheral stem cell transplantation (PSCT) rather than an autologous bone marrow transplantation (ABMT) because each patient had a marrow abnormality, either hypocellularity or tumor involvement. (shengsci.com)
  • The 30 patients who had no marrow metastases at the time of PSC harvesting had an actuarial event-free survival of 47%, while those 26 patients with marrow metastases had a significantly different actuarial event-free survival of 27% (P = .02). (shengsci.com)
  • CBV and PSCT for patients with relapsed Hodgkin's diseases who have marrow hypocellularity in traditional harvest sites or histopathologic evidence of BM metastases can result in long-term event-free survival. (shengsci.com)
  • Preemptive immunotherapy in childhood acute myeloid leukemia for patients showing evidence of mixed chimerism after allogeneic stem cell transplantation. (shengsci.com)
  • Screening for recurrently mutated genes in the mononuclear cell fraction revealed mutations in SF3B1 in 39 of 40 cases (97.5%), combined with TET2 and DNMT3A in 11 (28%) and 6 (15%) patients, respectively. (nih.gov)
  • The aim of this study was to assess whether the lnc-DC plays a role in patients with aGVHD by measuring its expression levels compared to non-aGVHD patients on specific time intervals following transplantation. (magiran.com)
  • Participants included 38 patients who underwent primary allogeneic bone marrow transplantation. (magiran.com)
  • Current surveillance strategies for patients with SDS and other leukemia predisposition syndromes rely on monitoring hematologic status by serial peripheral blood counts to identify worsening cytopenias and bone marrow examinations to identify morphologic changes or development of clonal chromosomal abnormalities 11 . (nature.com)
  • Between May 2005 and February 2010, 126 consecutive patients underwent intramyocardial cell injection. (tci-thaijo.org)
  • For a long time, stem cells have been utilized to renew the immune system for radiation or chemo- therapy treated patients. (bmrat.org)
  • In general, transplantation yields the best results when performed early in the course of the disease (ie, in an asymptomatic affected sibling of a child with a lysosomal storage disorder), in centers with experience in performing transplantations to treat inherited metabolic disorders, and in patients healthy enough to tolerate the conditioning and transplantation regimen. (medscape.com)
  • Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
  • En outre, il y avait une aug- mentation significative de la forme soluble du c-kit dans le sérum des patients atteints de pemphigus vulgaire actif par rapport aux témoins. (who.int)
  • All patients can evolve into trisomy 8+ MDS [12], cells, since acquiring adequate samples diagnosed with MDS have a reduced in which 8+ appears to confer a favour- for flow cytometric analysis renders the life expectancy compared with age- able prognosis [13]. (who.int)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • 11 Zhang S Zhang P Guo J, et al Enhanced cytoprotect1 and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function J . Eur J Cardiothorac Surg, 2004, 25(2):188-195. (xml-journal.net)
  • Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
  • Due to their secretory functions, differentiation capabilities, specific homing effects through chemotaxis, distinctive therapeutic potentials, and ex vivo expandability, cells have become an attractive reagent for advanced therapeutic strategies. (biomedcentral.com)
  • Therefore, the ability to modify cells and manipulate their functions according to intended therapeutic designs has been the central scientific interest in the field of biomedical research. (biomedcentral.com)
  • Cell surface engineering to provide new characteristics and functions to cells has drawn continuous interest from researchers in biomedical science as cell therapy has emerged as a prominent therapeutic strategy equivalent and complementary to the conventional therapeutic approaches. (biomedcentral.com)
  • Heart infarct in NOD-SCID mice: therapeutic vasculogenesis by transplantation of human CD34+cells and low dose CD34+KDR+ cells J . FASEB J 2004 18(12) 1392-1394. (xml-journal.net)
  • Recently, stem cells are being engineered to carry therapeutic reagents to target tumor sites. (bmrat.org)
  • Mesenchymal stem cells: immunology and therapeutic benefits. (ui.ac.id)
  • 2011. http://www.intechopen.com/books/stem-cells-in-clinic-and-research/mesenchymal-stem-cells-immunology-and-therapeutic-benefits . (ui.ac.id)
  • The isolated cells (2.5-7.5×10(5)) were resuspended in 300 microL of the patient's cerebrospinal fluid, and implanted in motor cortexes using a Hamilton syringe. (cellsurgicalnetwork.com)
  • On day 27, the patient's bone marrow was hypocellular and in morphologic remission with undetectable minimal residual disease and she was discharged 52 days after stem-cell transplantation. (ajmc.com)
  • At the time Grillo wrote his reference textbook, tracheal replacement using the tissue-engineered technique had not yet been applied to malignant tumors, as it required the use of the patient's own cells and because several months were required to construct the graft. (biomedcentral.com)
  • The stem cells are isolated from the patient's own blood and cultured. (tci-thaijo.org)
  • blood stem cell (PBSC) transplants, Barbara L. Haller, for which DNA was extracted from Patricia M. Denn, serum specimens collected at 6 and Christopher S. Hall,* Parvoviruses in 12 months, respectively, after trans- and Julie L. Gerberding plantation. (cdc.gov)
  • Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. (wikipedia.org)
  • CD34+ cells may be isolated from blood samples using immunomagnetic techniques and used for CD34+ transplants, which have lower rates of graft-versus-host disease. (wikipedia.org)
  • Children who received transplants showed improved growth rates and started to synthesize intact bone. (issca.us)
  • Moreover, children who received transplants approached growth curves similar to the children transplanted with allogeneic complete bone marrow [6]. (issca.us)
  • CD34 derives its name from the cluster of differentiation protocol that identifies cell surface antigens. (wikipedia.org)
  • Moreover, it seems that paracrine action of skeletal myoblasts facilitate neighboring cardiomyocytes to maintain their replicative potential and/or stimulate differentiation of native cardiac stem cells (8,9). (escardio.org)
  • Propagation and differentiation of neural stem cells at different O2 levels: comparative studies in vitro and in vivo. (remedicon.hu)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • 2016). Natural killer cell-based adoptive immunotherapy eradicates and drives differentiation of chemoresistant bladder cancer stem-like cells. (springer.com)
  • As an intergenic lncRNA, the lncDC was shown to regulate the human monocytes differentiation and antigen presenting cells (APCs) activation during immune responses. (magiran.com)
  • Repair of scar tissue constitutes a challenge for cardiac stem cell therapy due to lack of adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
  • NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
  • Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
  • In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
  • Together, these data suggest that CXCR4 plays an important role in donor cell engraftment. (biomedcentral.com)
  • Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)
  • In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
  • We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
  • However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
  • We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
  • Engraftment of donor-type MSC-derived osteoblasts was shown using bone specimens and microsatellite DNA marker analysis. (issca.us)
  • The use of base editing to generate universal, off-the-shelf CAR T cells is a promising approach for relapsed leukemia, with potential implications for the future of gene therapy. (ajmc.com)
  • Understanding of molecular mechanisms in natural killer cell therapy. (springer.com)
  • 2014). Human NK cells: From surface receptors to the therapy of leukemias and solid tumors. (springer.com)
  • She also actively participates as an academic member of the European Society of Gene and Cell Therapy and the International Society for Cellular Therapy, among others. (regenestem.com)
  • Previous studies have shown that children with acute myeloid leukemia (AML) who developed mixed chimerism (MC) were at high risk for relapse after allogeneic stem-cell transplantation (allo-SCT). (shengsci.com)
  • To understand the mechanisms that mediate germline genetic leukemia predisposition, we studied the inherited ribosomopathy Shwachman-Diamond syndrome (SDS), a bone marrow failure disorder with high risk of myeloid malignancies at an early age. (nature.com)
  • Moreover, cell surface engineering plausible for cardiac remodeling and the future prospective will be discussed at the end. (biomedcentral.com)
  • We tested whether shock wave (SW) offers additional benefits in improving left ventricular (LV) function after acute myocardial infarction (AMI) in rabbits receiving SW-treated autologous bone marrow-derived mononuclear cells (BMDMNCs) transplantation. (softwavetrt.com)
  • The team transduced healthy volunteer donor T cells using a lentivirus to express a CAR with specificity for CD7, which is expressed in T-cell ALL. (ajmc.com)
  • Finally, patient 3 is a 15-year-old boy who first presented with mixed-phenotype acute leukemia in 2016 and underwent a first allogeneic stem cell transplantation from a matched unrelated donor. (ajmc.com)
  • We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
  • Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
  • This way of making iPSCs from adult cells circumvents the need to destroy an embryo from living donor. (bmrat.org)
  • The Allogeneic bone-marrow transplantation represents the only curative treatment and requires a compatible donor HLA. (longdom.org)
  • We initially search a potential donor within the siblings of the patient by determining HLA type, true genetic identity card of the cells. (longdom.org)
  • Comparison of NOD-like receptors in limbal stem cell-derived corneal epithelial cells and in immortalized corneal epithelial cell line. (remedicon.hu)
  • that binds heparin and dermatan sulfate, and facilitates binding of B. burgdorferi to epithelial cells and glial cells [50]. (phosphatases.com)
  • For example, Vero cells, which were derived from monkey kidney epithelium [65], can be used as a representative of epithelial cells for studying GAGs-mediated adherence. (phosphatases.com)
  • mononuclear cells (PBMCs). (cdc.gov)
  • In- duced pluripotent stem cells have been used to create active T cells to support cancer immunotherapy. (bmrat.org)
  • This re- view discusses stem cell applications in transplantation, stem cell-based carriers, induced-pluripotent stem cells, can- cer stem cells, and potential of stem cells engineering to revolutionize cancer treatment. (bmrat.org)
  • These cells are called induced pluripotent stem cells (iPSCs). (bmrat.org)
  • HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
  • HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
  • In experimental settings, the ability of human HSCs to reconstitute hematopoiesis is measured by transplantation into genetically immunocompromised mice (e.g. (stemcell.com)
  • Upon transplantation into immune-deficient mice, SF3B1 mutated MDS-RS HSCs differentiated into characteristic ring sideroblasts, the hallmark of MDS-RS. (nih.gov)
  • Hematopoietic stem cells (HSCs) are very rare cells, representing approximately one in 100,000 bone marrow (BM) cells in the adult. (longdom.org)
  • The method of stimulating isolated cells with CSE in vitro has been explored and frequently applied to determine the direct causes in the relationships between cigarette smoking and cellular functions [ 15 ]. (hindawi.com)
  • In this study, in attempt to provide fresh information about the impact of CS on proliferation of EPCs to elucidate the pathophysiological mechanisms of the diseases related to CS in cellular level, we assessed the proliferation of EPCs after interfering the cells with a series of concentrations of CSE for various times of exposure in vitro . (hindawi.com)
  • Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
  • Southern analysis of DNA from the nonadherent cells produced by these LTC over a 6-wk period provided evidence of clonal evolution of LTC-IC in vitro. (jci.org)
  • CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
  • tissue colonization and multisystemic disease during mammalian infection, many different types of host cell lines can be employed to investigate Rucaparib manufacturer adherence [58-64]. (phosphatases.com)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • The percutaneous use of a combination of autologous adipose tissue complex (inclusive of its heterogeneous cell populations and native bioactive scaffold) and either HD-PRP or HD-PRP/BMAC combination appear as promising options for non-surgical management of Achilles tendonosis and partial thickness interstitial tears. (journalofprolotherapy.com)
  • Proteomics is a powerful approach to study the whole set of proteins expressed in an organism, organ, tissue or cell resulting in valuable information on physiological or pathological state of a biological system. (oaji.net)
  • Those are due to the unique characteristics of stem cells, such as immunological tolerance, migration, and tissue reparation. (bmrat.org)
  • Stem cells continue to play a role in repairing damaged tissue and replacing cells that are lost every day. (bmrat.org)
  • Side-by-side comparison of the biological characteristics of human umbilical cord and adipose tissue-derived mesenchymal stem cells. (ui.ac.id)
  • Cell Tissue Res. (ui.ac.id)
  • Cell Tissue Transplant Ther. (ui.ac.id)
  • The activation of these cells leads to the release of chemical mediators such as histamine and arachidonic acid metabolites, which increase vascular permeability and tissue swelling 9 . (bvsalud.org)
  • GALNS deficiency induces the accumulation of glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6-sulfate (C6S) in multiple tissues, particularly bone, cartilage, heart valves, and cornea, whereas β-galactosidase deficiency induces the accumulation of only KS in those tissues. (medscape.com)
  • Centeno CJ, Busse D, Kisiday J, Keohan C, Freeman M, Karli D: Increased knee cartilage volume in degenerative joint disease using percutaneously implanted, autologous mesenchymal stem cells. (e3ligase-signal.com)
  • Isolation and cultivation of mesenchymal stem cells from iliac crest bone marrow for further cartilage defect management. (ui.ac.id)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Organs and tissues are built up by specialized cells from the pool of stem cells that form shortly after fertilization. (bmrat.org)
  • Stem cells are widely defined by two main characteristics: the ability to self-renew (divide in a way that reproduces more identical stem cells) and to differentiate (to turn stem cells into specialized cells that form different organs and tissues). (bmrat.org)
  • In this investigation we sought to answer the question concerning the presence of mast cells in human and rat dental pulp tissues, under normal and inflammatory conditions. (bvsalud.org)
  • The histopathological changes such as the infiltration of inflammatory cells, edema, and fibrosis were also examined. (oaji.net)
  • It is possible that melatonin protects against IR-induced lung injury via the downregulation of this pathway and attenuation of inflammatory cells infiltration. (oaji.net)
  • CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
  • Mast cells play an important role in a variety of biological processes and actively participate in the inflammatory response. (bvsalud.org)
  • The role of mast cells in the inflammatory dental pulp response is not clear. (bvsalud.org)
  • Since mast cells are active during inflammatory responses it becomes crucial to find out if these cells are actually present and play a role in dental pulp under normal and inflammatory conditions either in humans or in rats. (bvsalud.org)
  • The proliferations of EPCs isolated from bone marrow of C57BL/6J mice were assessed by MTT after incubating the EPCs with a series of concentrations of CSE (1.0%, 2.5%, 5.0%, and 10.0%) for different times (3, 6, and 24 hours) as well as with 1.0% CSE in presence of 5-AZA-CdR for 24 hours. (hindawi.com)
  • Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
  • Early transplantation of bone marrow mononuclear cells promotes neuroprotection and modulation of inflammation after status epilepticus in mice by paracrine mechanisms. (fiocruz.br)
  • Adult stem cells were firstly isolated from bone marrow in mice (Spangrude, Heimfeld, and Weissman, 1988) and later in humans. (bmrat.org)
  • Shortly thereafter, it was demonstrated that intravenous injection of BM cells also provided radioprotection of lethally irradiated mice. (longdom.org)
  • These include bone marrow-derived cells, peripheral blood derived stem cells and skeletal myoblasts (3). (escardio.org)
  • The resistant state of skeletal myoblasts to ischemia renders these cells suitable candidates for repair of chronically infracted and failing heart (5). (escardio.org)
  • Estudio de la interacci n entre el sistema cannabinoide y el sistema serotonin gico en un modelo gen tico de depresi n por deficiencia del CB1R y en un modelo de depleci n farmacol gica de serotonina (5-HT) y en el alcoholismo materno fetal. (uba.ar)
  • CD133(+) stem cells are known to have the capacity to differentiate into neural lineages. (cellsurgicalnetwork.com)
  • Our bodies contain a pool of stem cells that have the ability to differentiate into any other cell type in the body. (bmrat.org)
  • Bone marrow is the place for the production of the hematopoietic cells which differentiate in to three groups: red blood cells, white blood cells and platelets. (longdom.org)
  • Bone marrow aspiration is carried out by a puncture on the level of the sternum or the posterior iliac peaks under local anesthesia by the doctor. (longdom.org)
  • He underwent lymphodepletion and received 1x106 BE-CAR7 T cells per kilogram. (ajmc.com)
  • This was associated with increased infiltration of macrophages, lymphocytes, and mast cells. (oaji.net)
  • Pemphigus vulgaris (PV) is a life-threate- disorders and to shed a light on the role of ning blistering skin disease in which pa- mast cells in autoimmune diseases [ 7 ]. (who.int)
  • There is a controversy in the literature whether mast cells are present in dental pulp. (bvsalud.org)
  • Our results showed that inflamed and non-inflamed dental pulps neither from humans nor from rats presented mast cells. (bvsalud.org)
  • It has been a matter of controversy in the literature whether mast cells are present in dental pulp. (bvsalud.org)
  • In addition there is a lack of information regarding the presence of mast cells in dental pulp of rats, which are animals used to study important aspects of the pathophysiology of dental pulp in vivo 6 . (bvsalud.org)
  • Mast cells play an important role in a variety of biological processes including allergic reactions, atherosclerosis and inflammation 7,8 . (bvsalud.org)
  • Therefore, the purpose of the present work was, under the same experimental conditions, to identify mast cells in healthy and inflamed dental pulp from rats and humans using toluidine blue histochemistry and immunohistochemistry techniques. (bvsalud.org)
  • Recent studies have shown that soluble factors elaborated by human T lymphocytes enhance erythroid burst formation by human peripheral blood null cells. (shengsci.com)