• In particular, stem cells, including mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs), and induced pluripotent stem cells (iPSCs), and immune cells, such as T-cells and Natural Killer (NK) cells, have been favored candidates for regenerative medicine and cell-based cancer immunotherapy, respectively. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs), or stromal stem cells, can differentiate into many different types of … In contrast, treatments that utilize MSCs from a patientâ s fat (adipose) sample have shown weak or unreliable responses. (tomeipowered.com)
  • As a result of over five decades of investigation, mesenchymal stromal/stem cells (MSCs) have emerged as a versatile and frequently utilized cell source in the fields of … MSC have been shown to differ-entiate in vitro into adipocytes, chon-drocytes, osteoblasts, myocytes, and ß-pancreatic islets cells. (tomeipowered.com)
  • The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. (tomeipowered.com)
  • Mesenchymal stem cells (MSCs) are an example of tissue or 'adult' stem cells. (tomeipowered.com)
  • The original mesengenic process pathway (Figure 1) was hypothesized and fashioned … Two opposing descriptions of so-called mesenchymal stem cells (MSCs) exist at this time. (tomeipowered.com)
  • The problem is what a mesenchymal stem cell (MSCs) is isn't always clearly defined, which has led to confusion in the scientific community, as well as public misunderstandings exploited by businesses marketing questionable cell-based treatments, the authors argue. (tomeipowered.com)
  • Properties of Mesenchymal … We found that … Platelets are known to enhance the wound-healing activity of mesenchymal stem cells (MSCs). (tomeipowered.com)
  • Among the cell types that can be used for this purpose, mesenchymal stem cells (MSCs) are considered as promising source of stem cells in personalized cell-based therapies. (tomeipowered.com)
  • Mesenchymal stem/stromal cells (MSCs) are multipotent cells that are emerging as the most promising means of allogeneic cell therapy. (tomeipowered.com)
  • Bone marrow-derived mesenchymal stem cells (BM-MSCs) were the first to be recognized and the most extensively studied for CVDs [ 3 ]. (biomedcentral.com)
  • ADSCs were first discovered and defined as mesenchymal stem cells (MSCs) isolated from processed lipoaspirate by Zuk and colleagues in 2001 [ 8 ]. (biomedcentral.com)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) from bone marrow play a critical role in osteochondral repair. (hindawi.com)
  • Mobilized pluripotent MSCs from the subchondral bone migrate into the defect filled with the clot, differentiate into chondrocytes and osteoblasts, and form a repair tissue over time. (hindawi.com)
  • The purpose of this review is to bridge the gap in our understanding between the basic science knowledge on MSCs and BMA and the clinical and technical aspects of marrow stimulation-based cartilage repair by examining available data on the role and mechanisms of MSCs and BMA in osteochondral repair. (hindawi.com)
  • Mesenchymal stem cells (MSCs) play a key role in articular cartilage repair. (hindawi.com)
  • They were first isolated from the bone marrow, and the potency of MSCs is currently being employed in the techniques of marrow stimulation for symptomatic small chondral defects. (hindawi.com)
  • In the orthopaedic field, additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation has been recently studied, since the bone marrow itself is both a source of MSCs, providing a cell population capable of chondrogenesis and of various growth factors stimulating cartilage repair [ 7 - 10 ]. (hindawi.com)
  • Moreover, the bone marrow clot provides a three-dimensional (3D) environment which supports the chondrogenesis of MSCs. (hindawi.com)
  • These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)
  • As the major cell precursors in osteogenesis, mesenchymal stem cells (MSCs) are indispensable for bone homeostasis and development. (nature.com)
  • Mesenchymal stem cells (MSCs) show trilineage differentiation because these cells differentiate into osteocytes, chondrocytes and adipocytes. (nature.com)
  • As primary sources of osteoblasts, MSCs show osteogenic functions that are closely associated with bone homeostasis and development. (nature.com)
  • Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. (biomedcentral.com)
  • The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs). (biomedcentral.com)
  • MSCs have been isolated from various tissues such as bone marrow (BM), umbilical cord blood, skeletal muscle and brain. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into bone, cartilage, fat and muscle cells and are being investigated for their utility in cell-based transplantation therapy. (biomedcentral.com)
  • A dose-response to QDs in rat bone marrow MSCs was assessed in Control (no-QDs), Low concentration (LC, 5 nmol/L) and High concentration (HC, 20 nmol/L) groups. (biomedcentral.com)
  • In addition, functional integration of QD labeled MSCs in an in vitro cardiomyocyte co-culture was assessed. (biomedcentral.com)
  • Finally, in a cardiomyocyte co-culture QD labeled MSCs were easy to locate and formed functional cell-to-cell couplings, assessed by dye diffusion. (biomedcentral.com)
  • In the present study, rat bone marrow MSCs were used to evaluate QD exposure on labeled MSC yield, QD retention and proliferation. (biomedcentral.com)
  • We assessed the ability to track QD labeled MSCs in an in vitro cardiomyocyte co-culture. (biomedcentral.com)
  • Mesenchymal stem/stromal cells (MSCs) are a reservoir for tissue homeostasis and repair that age during organismal aging. (encyclopedia.pub)
  • During aging of the organism, MSCs also age, and this implies an impairment of stem cell functions contributing to the progressive decrease in tissue maintenance and repair, a characteristic of the aging process. (encyclopedia.pub)
  • Due to the low abundance of MSCs in human adult tissues (about 1/10 6 cells in adult bone marrow and 1/10 3-4 cells in adipose tissue and umbilical cord) [ 16 ] , frequently ex-vivo expansion precedes therapeutic administration, to obtain a clinically relevant number of cells. (encyclopedia.pub)
  • The administration of bone marrow-derived mesenchymal stem cells (BMSCs) is emerging as a promising approach for I/R injury-induced myocardial infarction due to its multiple differentiation potential(7, 8). (researchsquare.com)
  • Stem cells, including embryonic stem cells (ESCs) and adult stem cells (ASCs), are cells capable of self-renewal and differentiation into a variety of phenotypes, and could be used for the treatment of heart failure [ 3 , 4 ]. (biomedcentral.com)
  • In particular, stem cells are highly sensitive to extracellular signals that play a critical role in mainten- ance of stem cell characteristics, differentiation, and interplay with somatic cells. (unicyte.ch)
  • Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
  • 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • Here, we investigate the cytotoxic effects of in vitro QD labeling on MSC proliferation and differentiation and use as a cell label in a cardiomyocyte co-culture. (biomedcentral.com)
  • The established dogma has however been challenged by recent findings suggesting that multipotent stem cells have a broader differentiation potential than previously thought. (biomedcentral.com)
  • These cells possess both differentiation plasticity (stemness) and tissue supportive functions (stromalness) that can coexist and overlap, with differences depending on tissue source, donor characteristics, culture conditions and delivery strategies, leading to alternative best fittings for the term "stem" or "stromal" [ 7 ] . (encyclopedia.pub)
  • Moreover, hEGFR KI/KI mice display accelerated chondrocyte and osteoblast differentiation, a phenotype that is also present in Egfr -/- mice and has not been previously described. (silverchair.com)
  • These cells have been shown to have the capability of differentiating into cardiomyocytes, vascular smooth muscle cells, and endothelial cells. (biomedcentral.com)
  • These cells have the potential to differentiate into cardiovascular lineages, such as cardiomyocytes (CMs), endothelial cells (ECs), and vascular smooth muscle cells (VSMCs) [ 7 ]. (biomedcentral.com)
  • Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
  • Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
  • He has been appointed as the Coordinator of various pan-European consortia including the large-scale integrated EU-projects FunGenES ("Functional Genomics of Engineered Embryonic Stem Cells), CRYSTAL ("Cryo-banking of stem cells for human Therapeutic Application"), ESNATS ("Embryonic stem cell-based Novel Alternative Testing Strategies"), and DETECTIVE - Detection of endpoints and biomarkers for repeated dose toxicity using in vitro systems. (wikipedia.org)
  • The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. (drcremers.com)
  • To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro . (drcremers.com)
  • 2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
  • Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
  • This review will examine the clinical potential of several stem and progenitor cells that may be utilised to regenerate defunct or damaged vasculature and restore blood flow to the ischaemic tissue. (biomedcentral.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • CC-ASC generated epithelial cells with undifferentiated morphology in all culture methods, among which CC-ASC in 3D culture supported the highest cell doubling (cells doubled 9.0 times compared to cells doubled 4.9 times in control) while maintained the percentage of putative limbal stem/progenitor cells compared to the control. (drcremers.com)
  • Mei H, González S, Nakatsu MN, Baclagon ER, Chen FV, Deng SX (2017) Human adipose-derived stem cells support the growth of limbal stem/progenitor cells. (drcremers.com)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • Pasca , assistant professor of psychiatry and behavioral sciences, and Scherrer , assistant professor of anesthesiology, perioperative and pain medicine and of neurosurgery, were named 2017 New York Stem Cell Foundation Robertson Investigators. (stanford.edu)
  • The first expanded access program (EAP) of cord blood stem cell therapy for cerebral palsy launched in the United States at Duke University in Oct. 2017, under FDA registration as clinical trial NCT03327467. (biohellenika.gr)
  • We quantified CXCL10 in human CKD plasma and tested the response of human iPSC-derived cardiomyocytes and primary cardiac fibroblasts to serum from CKD donors. (biomedcentral.com)
  • In humans, we found increased plasma CXCL10 concentrations in advanced CKD, and identified the production of CXCL10 in cardiomyocytes and cardiac fibroblasts. (biomedcentral.com)
  • First identified in embryonic stem cells, SEs mediate pluripotent state maintenance by promoting the expression of pluripotent identity genes, including Oct4 , Sox2 , Nanog and Klf4 . (nature.com)
  • We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)
  • In addition, the authors also review future strategies in DN treatment such as transcriptional reprogramming of mature adult kidney cells into uncommitted induced pluripotent stem cells for renal repair and therapeutics. (emjreviews.com)
  • The cells giving rise to the foetus, are called pluripotent embryonic stem cells, since they can form every cell type in the body. (biomedcentral.com)
  • A recent study indicates that even adult humans might harbor cells resembling pluripotent embryonic stem cells. (biomedcentral.com)
  • Human Induced Pluripotent Stem Cell-Derived Pericytes as Scalable and Editable Source to Study Direct Lineage Reprogramming Into Induced Neurons. (antibodiesinc.com)
  • Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
  • He achieved a first functional characterization of cardiomyocytes developed from embryonic stem cells which is an important prerequisite for their subsequent use in regenerative medicine. (wikipedia.org)
  • The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
  • The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
  • Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
  • He was the first scientist worldwide to perform electrophysiological and functional experiments on stem cells and differentiated phenotypes thereof. (wikipedia.org)
  • They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)
  • In vascular medicine, various stem cells and adult progenitors have been highlighted as having a vasoreparative role in ischaemic tissues. (biomedcentral.com)
  • However, stem cell-based transplantation therapy faces the problems of poor donor cell engraftment and survival in the ischemic heart. (biomedcentral.com)
  • The final result is that, after MI, billions of cardiomyocytes (CMs) with limited proliferation capacity are lost and substituted by heterogeneous collagen-rich fibrotic scar tissue [ 2 ] [ 3 ] . (encyclopedia.pub)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. (biomedcentral.com)
  • Beginning with studies on cellular signal transduction, and starting with works on signalling pathways in different cells (g-protein phosphorylation, electrophysiology of channels) he focussed on embryonic stem cells and has since defined many important basic aspects of both fundamental research and clinical applications. (wikipedia.org)
  • Among progressing the use of stem cells for translational purposes, aiming at tapping the potential of stem cell biology towards clinical application, Hescheler actively promotes the vision of EPAA through innovative alternative testing strategies, applying stem cells towards toxicity testing. (wikipedia.org)
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • Implications of findings from both translational and clinical studies using BMA concentrate-enhanced marrow stimulation are discussed. (hindawi.com)
  • Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
  • Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
  • Expecting parents mut be informed about the current and future use of cord blood stem cells and decide for family use or for public donation. (biohellenika.gr)
  • Cerebral Palsy Expanded Access Program in Europe November 2019 Frances Verter, PhD Cerebral Palsy patients and families living in Europe now have expanded access to a program of therapy with cord blood stem cells. (biohellenika.gr)
  • This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)
  • Rejuvenation therapies aim to reverse or repair age-related cellular changes such as molecular waste, calcification , tissue stiffening , loss of stem cell function , genetic alterations, and impaired energy production . (fightaging.org)
  • The relationship between monocyte subset and macrophage phenotype is still to be resolved, however tissue macrophages clearly have diverse functions and are key inflammatory mediators [ 23 ]. (biomedcentral.com)
  • 1. Activation of adipose tissue and related mesenchymal stem cells: promotion of anti-inflammatory properties through SOX2 transcription factor and HuR RNA-binding protein modulation. (neurogelenmarche-usa.org)
  • Human MSC are easy to isolate from small aspirate of bone marrow via their adherence ability. (biomedcentral.com)
  • A focus is on the steps of mobilization of cells from the subchondral bone and repair tissue formation, including adherence of the bone marrow clot to the subchondral bone. (hindawi.com)
  • He became the first researcher to accomplish an electrophysiological characterization of stem cells and was also among the first scientists in Germany obtaining permission to do research on human embryonic stem cells. (wikipedia.org)
  • In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
  • Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • We treat acute myocardial infarction by targeting exogenous bone marrow-derived stem cells (expressing CD45) or endogenous CD34-positive cells to injured cardiomyocytes (expressing myosin light chain. (regenerativemedicine.net)
  • The severity of the phenotypes correlates with the expression levels of the hEGFR KI allele, which is not efficiently expressed in epithelial and bone cells, but is expressed at similar and even higher levels as the endogenous Egfr in brain and heart. (silverchair.com)
  • The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
  • The myocardial nicheplays a critical role in directing the function and fate of cardiomyocytes;therefore, engineering a biomimetic scaffold holds excellent promise.We produced an electroconductive cardiac patch of bacterial nanocellulose(BC) with polypyrrole nanoparticles (Ppy NPs) to mimic the naturalmyocardial microenvironment. (ibecbarcelona.eu)
  • The additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation is thought to enhance cartilage repair as it may provide both an additional cell population capable of chondrogenesis and a source of growth factors stimulating cartilage repair. (hindawi.com)
  • Such enhanced techniques of marrow stimulation have been shown to improve articular cartilage repair in both animal models and patients. (hindawi.com)
  • These processes influence gene expression patterns or cellular phenotypes and disease states with no underlying change in DNA sequence. (emjreviews.com)
  • Adipose-derived stem cells (ADSCs) are easily obtained and expanded, and have emerged as a novel source of adult stem cells for the treatment of cardiovascular diseases. (biomedcentral.com)
  • Myocardial infarction (MI) is one of the clusters of numerous other cardiovascular diseases and occurs due to the blockage of the coronary artery delivering blood (ischemia) to the ventricle with consequent oxygen shortage to the contractile cells (cardiomyocytes) [ 1 ] . (encyclopedia.pub)
  • In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
  • Bone-targeting ZBTB16 overexpression had a therapeutic effect on the decreased bone density and remodeling capacity of Brd4 fl/fl Prx1 -cre mice and osteoporosis (OP) models. (nature.com)
  • A bone marrow clot forms within the cartilage defect either as a result of marrow stimulation or during the course of the spontaneous repair of osteochondral defects. (hindawi.com)
  • If bone marrow fills a cartilage defect either as a result of marrow stimulation for chondral defects or the course of the spontaneous repair of osteochondral defects, a bone marrow clot forms within the cartilage defect. (hindawi.com)
  • Growing evidence suggests that transcriptional regulators and secreted RNA molecules encapsulated within membrane vesicles modify the phenotype of target cells. (unicyte.ch)
  • Stem cells are known to work via a variety of methods to heal tissue depending on where the stem cells come from and into which tissue it is being placed. (drcremers.com)
  • If members of the family do not match, the next options are to seek a match from the registries of unrelated adult donors, or from a registry of donated cord blood. (biohellenika.gr)
  • But another factor parents need to consider, is that stem cell transplants have fewer complications and better survival rates with donors who are not just a match on the HLA types, but who are related to the patient. (biohellenika.gr)