Bone marrow derived stem cells. Medical search. Frequent questions

In this study, we investigated the modulation of pDC differentiation in Flt-3 ligand (Flt3L)-supplemented BM cultures, using highly purified mesenchymal stem cells (MSCs) that were FACS-isolated from murine BM based on surface marker expression and used after in vitro expansion. (nih.gov)
Cytokines and growth factors (interleukin-8 transforming growth factor-beta stromal cell-derived factor-1alpha and vascular endothelial growth factor) that mediate endogenous bone regeneration were also produced by expanded MSCs from SCD patients. (bioskinrevive.com)
Mesenchymal stem cells (MSCs) are multipotent stem cells with a high capacity for self-renewal. (mbpinc.net)
In addition, bone marrow (BM)-derived mesenchymal stem cells (MSCs) have been suggested as an effective therapy for hepatic fibrosis and cirrhosis. (elsevierpure.com)
The aim of this study was to determine whether the combination treatment of simvastatin plus MSCs (Sim-MSCs) could have a synergistic effect on hepatic fibrosis in a thioacetamide (TAA)-induced cirrhotic rat model and hepatic stellate cells (HSCs). (elsevierpure.com)
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. (ijbs.com)
In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differentiate into various mesodermal lineages including osteoblast/osteocyte, chondrocyte, and adipocyte that are crucial for bone metabolism. (ijbs.com)
While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. (ijbs.com)
To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. (ijbs.com)
We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. (ijbs.com)
We describe a novel therapeutic approach for cirrhosis using mesenchymal stem cells (MSCs) and colony‐stimulating factor‐1‐induced bone marrow‐derived macrophages (id‐BMMs) and analyze the mechanisms underlying fibrosis improvement and regeneration. (birmingham.ac.uk)
Mouse MSCs and id‐BMMs were cultured from mouse bone marrow and their interactions analyzed in vitro. (birmingham.ac.uk)
Intravital imaging showed that after combination cell administration, a large number of id‐BMMs, which phagocytosed hepatocyte debris and were retained in the liver for more than 7 days, along with a few MSCs, the majority of which were trapped in the lung, migrated to the fibrotic area in the liver. (birmingham.ac.uk)
Indirect effector MSCs and direct effector id‐BMMs synergistically improved cirrhosis along with host cells in mice. (birmingham.ac.uk)
The key attributes like isolation efficiency, cell yield, colony-forming unit-fibroblast (CFU-F) frequency, phenotypic characteristics, and multi-lineage differentiation capacity were determined for the isolated MSCs from bone marrow specimens. (journalcra.com)
Further, when those MSCs were analyzed for their specific marker expression using flowcytometry, they were found to be positive for CD29, CD73, CD90, and CD105 and negative for CD34 and CD45 stem cell markers respectively. (journalcra.com)
Apart from this, it was observed that those MSCs, when induced to differentiate into cardiomyocytes, they successfully differentiated into cardiomyocyte like cells with flat, wide, myotube like structure with sarcomeric organization, which were later confirmed to be cardiomyocytes by qRT-PCR. (journalcra.com)
Thus, this study shows that MSCs were isolated from human bone-marrow and subsequently differentiated into cardiomyocytes with 100% success rate. (journalcra.com)
Administration of bone tissue marrow-derived mesenchymal stem cells (MSCs) can be an innovative strategy for the treating a variety of diseases that aren't curable by current therapies including center failure. (bio-biz-navi.com)
This review content summarizes protocols to isolate and broaden bone tissue marrow-derived MSCs in 47 latest scientific studies of MSC-based therapy that have been released after 2007 onwards and supplied sufficient methodological details. (bio-biz-navi.com)
1 Launch Recent research provides extensively proven the potential of bone tissue marrow- (BM-) produced mesenchymal stem cells (MSCs) for regenerative remedies in a variety of organs like the center [1]. (bio-biz-navi.com)
Because the initial scientific trial of BMC shot in 1995 [3] a lot more than 2 0 sufferers have been implemented with allogeneic or autologous MSCs for the treating various illnesses including graft-versus-host disease hematologic malignancies cardiovascular illnesses neurologic illnesses autoimmune diseases body organ transplantation refractory wounds and bone tissue/cartilage flaws [4]. (bio-biz-navi.com)
MSCs stand for 0.001-0.01% of BM mononuclear cells or lower [8]. (bio-biz-navi.com)
Confocal and transmission electron microscopy demonstrate that mono-SMBGs are internalized by human bone marrow (MSCs) and adipose-derived stem cells (ADSCs) and located within cell vesicles and in the cytoplasm. (ox.ac.uk)
Mesenchymal stem cells (MSCs) have been considered promising tools for new clinical concepts in supporting cellular therapy and regenerative medicine. (cellmolbiol.org)
In this study we compared MSCs derived from three different sources (iliac crest bone marrow (ICBM), adipose tissue (AT), and (RIA)) regarding the morphology, the success rate of isolating MSCs, colony frequency, expansion potential, osteogenic and chondrogenic differentiation capacity. (cellmolbiol.org)
MSCs were isolated from three different sources and flow cytometric analyses were performed for cell characterization. (cellmolbiol.org)
Colony-forming unit-fibroblast (CFU-F) assay and population doubling time (PDT) were evaluated for MSCs derived from three different sources and differentiation potential of RIA, ICBM-, and AT-MSCs were determined by staining. (cellmolbiol.org)
Flow cytometry analysis indicated that RIA- and AT-derived MSCs have more homogenous populations than ICBM-MSCs. (cellmolbiol.org)
Studies on therapeutic administration of bone marrow-derived mesenchymal stem cells (MSCs) in ALI caused by the viral infection have been limited in number and have shown conflicting results. (unair.ac.id)
Methods: MSCs were prepared from the bone marrow of 9 to 12 week-old BALB/c mice. (unair.ac.id)
Conclusion: The administration of MSCs prevented further lung injury and inflammation, and enhanced alveolar cell type II and I regeneration, while it did not significantly affect viral proliferation and mouse morbidity and mortality. (unair.ac.id)
Recently, mesenchymal stem cells (MSCs) have been suggested for use in the cell-based treatment of cartilage lesions. (researchgate.net)
These correlations were not observed in group B. Conclusion: Injection of MSCs with marrow stimulation treatment was encouraging in patients older than 50 years compared with patients treated with marrow stimulation treatment alone, especially when the lesion size was larger than 109 mm(2) or a subchondral cyst existed. (researchgate.net)
In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
Severe joint injuries can be career-ending for horses, but veterinarians have been using regenerative medicine routinely-specifically, mesenchymal stem cells (MSCs)-to address these injuries in their practices, determining optimal approaches. (thehorse.com)
He addressed not only the effects of MSCs in the equine joint but also the methods of obtaining and processing these cells. (thehorse.com)
Frisbie stated that although clinical sources of MSCs include both bone marrow and adipose (fat) tissue, bone marrow is the superior source. (thehorse.com)
MesenCult™ Adipogenic Differentiation Medium (Human) is specifically formulated for the in vitro differentiation of primary human mesenchymal stromal cells, and hPSC-derived mesenchymal progenitor cells (also known as mesenchymal stem cells or MSCs) into adipogenic lineage cells. (stemcell.com)
This kit is suitable for the differentiation of MSCs derived from human bone marrow (BM), adipose tissue, umbilical cord, or pluripotent stem cells (PSCs) that have been previously culture-expanded in serum- and animal component-free medium (e.g. (stemcell.com)
Adipogenic differentiation of human bone marrow-derived MSCs using MesenCult™ Adipogenic Differentiation Medium (Human) or a competitor medium. (stemcell.com)
Mesenchymal Stem Cells (MSCs) are a heterogeneous subset of stromal stem cells that originate from the mesoderm layer of the fetus. (tocris.com)
We have also developed grafting of exogenous, bone marrow-derived MSCs as a local, targeted therapy of GBM. (lu.se)
To evaluate the number of adverse events or severe adverse event assessed by Common Terminology Criteria For Adverse Events (CTCAE) of intravenous infusion of human allogeneic BM-derived MSCs product StromaForte for all study participants. (who.int)

The use of mesenchymal stem cells or progenitor cells has been advocated for treatment of many musculoskeletal diseases in the equine. (ivis.org)
Bone marrow and adipose tissue have emerged as excellent sources of progenitor cells. (ivis.org)
In summary our results indicate that infusion of BMMCs enriched with stem/progenitor cells is a safe and effective treatment for the early stages of ONFH in SCD patients. (bioskinrevive.com)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
Most type II PNH cells (total lack of GPI-linked protein) are due to a frame shift mutation occurring in the early hematopoietic progenitor cells, resulting in same mutation in all blood cell lines. (medscape.com)
Downloaded from periodic thymic colonization by progenitor cells guarantees stage. (lu.se)

The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. (nature.com)
It is well-described that leptin receptor (LepR) + perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. (nature.com)
These Fgfr3-creER -marked endosteal stromal cells contribute to a stem cell fraction in young stages, which is later replaced by Lepr-cre -marked stromal cells in adult stages. (nature.com)
Further, Fgfr3 + endosteal stromal cells give rise to aggressive osteosarcoma-like lesions upon loss of p53 tumor suppressor through unregulated self-renewal and aberrant osteogenic fates. (nature.com)
These Fgfr3 + stem/stromal cells with OCT identities are abundant in the young bone marrow and depleted in the old bone marrow, denoting their transitional nature. (nature.com)
Overall, our findings indicate that Fgfr3 + endosteal stem/stromal cells with OCT identities dictate active and aggressive osteogenesis, identifying these cells as an important regulator of long-term bone homeostasis. (nature.com)
Bone marrow-derived mesenchymal stem/stromal cells (BMSCs) are fundamental to bone regenerative therapies, tissue engineering, and postmenopausal osteoporosis. (elsevierpure.com)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
We have identified and isolated mesenchymal stromal cells and studied some of their functions. (lu.se)
A Longitudinal Open-Label Multi-center Single-Arm Phase I/IIa Study to Evaluate the Safety and Preventive Effect on Cardiovascular Events of Human Allogenic Bone-Marrow-Derived Mesenchymal Stromal Cell Product, StromaForte, in Study Participants. (who.int)

liver cells and endothelial cells. (mbpinc.net)
The capacities of proliferation, migration, and repair of human umbilical vein endothelial cells were enhanced by BMSCs supernatant. (drcalapai.com)

Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers. (lu.se)
Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers significant implications for the study of cartilage biology and the discovery of potential therapeutic interventions for cartilage-related and associated disorders. (lu.se)
Bone marrow mesenchymal stem cells (BMSCs) have been reported to attenuate myocardial I/R injury via their paracrine effects, which can be enhanced by hypoxic preconditioning. (researchsquare.com)
We hypothesized that the donor heart preservation with hypoxic conditioned medium derived from BMSCs (CM-BMSCs) would improve post-transplant graft function. (researchsquare.com)
The administration of bone marrow-derived mesenchymal stem cells (BMSCs) is emerging as a promising approach for I/R injury-induced myocardial infarction due to its multiple differentiation potential(7, 8). (researchsquare.com)
Bone marrow mesenchymal stem cells (BMSCs) have potential therapeutic benefits for the treatment of endometrial diseases and injury. (biologists.com)
BMSCs interact with uterus parenchymal cells by direct contact or indirect secretion of growth factors to promote functional recovery. (biologists.com)
Mouse bone marrow -derived mesenchymal stem cells (BMSCs) were selected and BMSC- exosomes (BMSC-EXO) were extracted and then identified. (bvsalud.org)
This study evaluated the effect of bone marrow-derived stem cells (BMSCs) on degenerative changes of TMJ associated with bovine collagen type II (BCII) induced-arthritis. (thestemcellfoundation.com)
Donor variation among patients, cell heterogeneity, and unpredictable capacity for differentiation reduce effectiveness of BMSCs for regenerative cell therapies. (elsevierpure.com)
The cell surface glycoprotein CD24 exhibits the most prominent differential expression during osteogenic versus adipogenic differentiation of human BMSCs. (elsevierpure.com)
In undifferentiated human BMSCs, CD24 cell surface expression is variable among donors (range: 2%-10%) and increased by two to fourfold upon osteogenic differentiation. (elsevierpure.com)
RNAseq analysis of CD24pos and CD24neg BMSCs identified a limited number of genes with increased expression in CD24pos cells that are associated with cell adhesion, motility, and extracellular matrix. (elsevierpure.com)
Hence, expression of the cell surface glycoprotein CD24 identifies a subpopulation of human BMSCs with reduced capacity for proliferation and extracellular matrix mineralization. (elsevierpure.com)
Functional specialization among BMSCs populations may support their regenerative potential and therapeutic success by accommodating cell activities that promote skeletal tissue formation, homeostasis, and repair. (elsevierpure.com)
Bone marrow-derived mesenchymal stem cells (BMSCs) have been reported to have a potential therapeutic role on SAP, but the specific mechanism is unclear. (drcalapai.com)
BMSCs-derived exosomal miR-138-5p reduces neuron injury following IS in vivo. (biomedcentral.com)

Comparison of toxicity of benzene metabolite hydroquinone in hematopoietic stem cells derived from murine embryonic yolk sac and adult bone marrow. (cdc.gov)

The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. (scielo.br)
On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)

Additionally, it will explain the techniques of collecting, packaging, and transporting bone marrow aspirates for collection, expansion, and 150 injections into diseased tissues. (ivis.org)

Monodispersed bioactive glass submicron particles and their effect on bone marrow and adipose tissue-derived stem cells. (ox.ac.uk)

One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
Their easy accessibility as well as exceptional genomic stability and their capacity for tissue repair with few immunological and ethical issues make them the favorable cell type for experimental and clinical use. (mbpinc.net)
However, the tissue distribution of the injected cells remains unknown. (stemcellclinicsofamerica.net)
Ethical and practical considerations limit the availability of human fetal-derived neural tissue and highlight the need to consider alternative sources of human NSCs. (ox.ac.uk)
Differentiation potential cell therapy stem cells regenerative medicine orthopedic tissue engineering. (cellmolbiol.org)
An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
The aim of this study was to compare the osteogenic potential of mesenchymal stem cells obtained from bone marrow (BM-MSC) with those extracted from adipose tissue (AT-MSC) of adult dogs. (scielo.br)
Tissue- engineered fabrication of an osteochondral composite graft using rat bone marrow-derived mesenchymal stem cells. (bvsalud.org)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
The applicant is responsible for a brain tumor tissue biobank which provides the basis for the studies on human GBM tumor cells and stroma. (lu.se)
Acute leukopenia, with almost total absence of granular leukocytes, leukoblastic groups and lymphoid tissue in the bone marrow, was reported in the case of a 36-year-old chemist who had worked with radium for 14 years (Reitter and Martland 1926). (cdc.gov)

MiR-29a-3p was enriched in hBMSC-derived Exo, and the Exo from miR-29a-3p mimics transfected hBMSCs could inhibit laryngocarcinoma cell malignant phenotypes in vitro and prevent tumor progression in vivo . (hindawi.com)
The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
As a third major line of research, we explore, in collaboration with associate professor Henrik Ahlénius group if GBM cells can be reprogrammed to neuron like cells in vitro and in vivo. (lu.se)
Although other irritants in the smoke may have contributed to the incident, there is supporting evidence that stable strontium can stimulate the release of histamine from mast cells in vitro (ATSDR 2001e). (cdc.gov)

Introduction Stem cell therapy with bone marrow-derived mononuclear cells (BMMCs) is an option for improving joint function in osteonecrosis of the femoral head (ONFH). (bioskinrevive.com)

The transplantation of bone marrow-derived mesenchymal stem cells (BMMSCs) alleviates neuropathology and improves cognitive deficits in animal models with Alzheimer's disease. (biomedcentral.com)
Other treatment options for patients with enzyme or protein deficiencies include substrate reduction therapy, gene therapy, and bone-marrow derived stem cell transplantation. (wikipedia.org)
The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
Stem cell transplantation is the removal of stem cells (undifferentiated cells) from a healthy person and their injection into someone who has a serious blood disorder. (msdmanuals.com)
Overview of Transplantation Transplantation is the removal of living, functioning cells, tissues, or organs from the body and then their transfer back into the same body or into a different body. (msdmanuals.com)
But researchers think that these stem cells have the most potential for producing different kinds of cells and for surviving after transplantation. (msdmanuals.com)
These stem cells are most often used for transplantation. (msdmanuals.com)

This may result in clinicians opting out of pursuing this choice of stem cell therapy. (ivis.org)
Host macrophages and neutrophils infiltrated after combination therapy and contributed to liver fibrosis regression and promoted regeneration along with administered cells. (birmingham.ac.uk)
Our guide highlights the use of small molecules in stem cell research and cell therapy and lists relevant products. (tocris.com)
Cell Therapy for Parkinsons Disease: What Next? (lu.se)

Another group of rats received i.c. injection of EdU-labeled allogeneic penile smooth muscle cells (PSMCs). (stemcellclinicsofamerica.net)
Allogeneic adipose-derived stem cells rescue irradiation bone marrow cells via secretion of pro-survival and hematopoietic factors. (eurekalert.org)

Stem cells from umbilical cords are usually used only in children because umbilical cord blood does not contain enough stem cells to use in adults. (msdmanuals.com)
Stem cells can be obtained from the blood in the umbilical cord or placenta after a baby is born. (msdmanuals.com)

Previous studies indicated that exosomal miR-29a-3p derived from human bone mesenchymal stem cells (hBMSCs) could efficiently suppress cell migrative ability and vasculogenic mimicry in glioma [ 15 ]. (hindawi.com)
Due to its role in laryngocarcinoma, we speculated that the exosomal miR-29a-3p derived from hBMSCs might also exert a potential impact in cancer progression. (hindawi.com)
Mouse bone marrow derived mesenchymal stem cells-secreted exosomal microRNA-125b-5p suppresses atherosclerotic plaque formation via inhibiting Map4k4. (bvsalud.org)

They can develop into specialized cells of the body such as chondrocytes/osteoblasts/adipocytes. (mbpinc.net)
They can differentiate into cells of the mesodermal lineage, such as adipocytes, and osteocytes. (tocris.com)

We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
It is essential that the cells be subculture BEFORE reaching confluence as post-confluent cells exhibit changes in morphology, slower proliferation, and reduced differentiation after passaging. (ubpone.com)
Downregulated genes are associated with cell cycle regulation, and biological assays revealed that CD24pos cells have reduced proliferation. (elsevierpure.com)
We have already acquired proof-of-principle showing that reprogramming towards astrocytic fate causes morphological changes, reduces proliferation and bestow astrocytic function in human GBM cell lines. (lu.se)
Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids. (tocris.com)

The role of adult bone marrow-derived stem cells in choroidal neovascularization. (medscape.com)

Background: Chondrocytes are the primary cells responsible for maintaining cartilage integrity and function. (lu.se)

Postnatal astrocytes promote neural induction from adult human bone marrow-derived stem cells. (ox.ac.uk)

Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
They also have good pluripotential (the ability to become other cell types), but the cost of allogenic cells are high, and little is known about how these cells will differentiate. (thehorse.com)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)

Here we show that bone marrow endosteal SSCs are defined by fibroblast growth factor receptor 3 ( Fgfr3 ) and osteoblast-chondrocyte transitional (OCT) identities with some characteristics of bone osteoblasts and chondrocytes. (nature.com)
In this study, we report a novel population of SSCs with osteoblast-chondrocyte transitional (OCT) identities in the bone marrow endosteal space, which highly expresses Fgfr3 and contributes to both normal and aberrant osteogenesis. (nature.com)

However, for cell therapies MSC have to be expanded and/or manipulated to obtain a sufficient amount of cells that can be subsequently used for treatment. (biomedcentral.com)
Neural stem cells (NSCs) have generated considerable interest because of their potential as a source of defined cells for drug screening or cell-based therapies for neurodegenerative diseases. (ox.ac.uk)
Adult human bone marrow (BM) is the major source of mesenchymal stem cells (MSC) for cell-based therapies. (journalcra.com)
And hence, this study suggests that human bone marrow is a potential source of mesenchymal stem cells that could be differentiated in to ccardiomyocytes and used for cell based therapies for treating various cardiovascular diseases. (journalcra.com)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)

Fibrosis regression, liver regeneration, and liver‐migrating host cells were evaluated. (birmingham.ac.uk)
Bladder regeneration studies have yielded inconclusive results possibly due to the use of unfavorable cells and primitive scaffold design. (northwestern.edu)
We hypothesized that human mesenchymal stem cells seeded onto poly(1,8-octanediol-co-citrate) elastomeric thin films would provide a suitable milieu for partial bladder regeneration. (northwestern.edu)

Because of their ready availability, their ability to be easily expanded, and reports of neural potential, bone marrow-derived populations have become the focus of intense study with regard to their potential clinical utility. (ox.ac.uk)
Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)

Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt's macular dystrophy: follow-up of two open-label phase 1/2 studies. (medscape.com)

The exosomes (Exo) from human bone marrow mesenchymal stem cells (hBMSCs) after the transfection of miR mimics/inhibitor/si-PTEN were isolated and used to stimulate M4E and Hep2 cells. (hindawi.com)
Human MSC are easy to isolate from small aspirate of bone marrow via their adherence ability. (biomedcentral.com)
Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
In this study we asked if human MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
GENERAL INFORMATION: GFP HBMMSCs (UBP-0050) are isolated from human Bone Marrow tissues and demonstrated with spindle-shaped-, and fibroblast-like cells. (ubpone.com)
In this study, we demonstrate the monoclonal neural and mesodermal potential of adult human bone marrow mesenchymal cells. (ox.ac.uk)
The FDA has approved ERTs that are derived from other human cells, animal cells (i.e. (wikipedia.org)
In the present study, six samples of human bone-marrow were collected. (journalcra.com)
Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
PyV-induced tumorigenesis in laboratory animals, by simian virus 40 (SV40) or by multiple human PyVs, such as JC virus (JCV), is used extensively as a cell transformation model. (cdc.gov)
Because the embryos then lose the ability to grow into a complete human being, the use of stem cells from embryos is controversial. (msdmanuals.com)

In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. (tocris.com)
Our Stem Cells review gives an overview of the use of small molecules in the control of stem cell growth & differentiation and somatic cell reprogramming. (tocris.com)
Due to its location on the X chromosome, and due to X inactivation in female somatic cells, only one mutation is required in either males or females to abolish the expression of GPI-linked proteins. (medscape.com)

Although our approach has several advantages, further investigation is required to address its limitations, such as the potential differences between chondrocytes generated using our protocol and those derived from other established methods, and to refine the model for broader applicability and clinical translation. (lu.se)
However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. (biomedcentral.com)
Stem Cells (ADSC's) for clinical research and deployment. (stemcellclinicsofamerica.net)
Recent evidence suggests that pharmacological treatment devoted to regulating stem cell function is a potential new therapeutic strategy that is drawing nearer to clinical practice. (elsevierpure.com)
Purpose: To compare the clinical outcomes of MSC injection and arthroscopic marrow stimulation treatment with those of arthroscopic marrow stimulation treatment alone for the treatment of OLTs in older patients. (researchgate.net)
Patients were divided into 2 groups: 35 patients (37 ankles) treated with arthroscopic marrow stimulation treatment alone (group A) and 30 patients (31 ankles) who underwent MSC injection along with arthroscopic marrow stimulation treatment (group B). Clinical outcomes were evaluated according to the visual analog scale (VAS) for pain, the American Orthopaedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot Scale, and the Roles and Maudsley score. (researchgate.net)
The name of the disorder is a descriptive term for the clinical consequence of red blood cell (RBC) breakdown with release of hemoglobin into the urine, which manifests most prominently as dark-colored urine in the morning (see image below). (medscape.com)
3. Have a clinical history of malignancy within 2.5 years (i.e., study participants with prior malignancy must be cancer free for 2.5 years) except curatively treated basal cell carcinoma, melanoma in situ, or cervical carcinoma. (who.int)

In conclusion, hBMSC-derived Exo with upregulated miR-29a-3p inhibited laryngocarcinoma progression via regulating PTEN, providing a potential diagnostic and therapeutic target in this disease. (hindawi.com)
With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
Once inside the body the vector introduces the therapeutic gene into host cells, and the protein encoded by the newly inserted gene is then produced by the body's own cells. (wikipedia.org)
Spherical monodispersed bioactive particles are potential candidates for nanocomposite synthesis or as injectable particles that could be internalized by cells for the local sustained delivery of inorganic therapeutic ions (e.g., calcium or strontium). (ox.ac.uk)

In this study we tracked i.c.-injected adipose-derived stem cells (ADSCs) in various tissues. (stemcellclinicsofamerica.net)
The results show that ADSCs exited the penis within days of i.c. injection and migrated preferentially to bone marrow. (stemcellclinicsofamerica.net)
Allogenicity did not affect the bone marrow appearance of ADSCs at either 2 or 7 days, whereas CN injury reduced the number of ADSCs in bone marrow significantly at 7 but not 2 days. (stemcellclinicsofamerica.net)

The authors feel that it is important to aspirate bone marrow at the region of the tuber coxae located one-half of the way between the dorsal, ventral, and cranial border and caudal border. (ivis.org)
Flow cytometry analysis showed a significantly higher number of hematopoietic stem/endothelial progenitor cell markers in concentrated BMMCs when compared with bone marrow aspirate indicating an enrichment of these cell types. (bioskinrevive.com)
Direct aspirate using the cells straight from harvestwithout further processing. (thehorse.com)

The discovery of this new class of SSCs is based on the conjunction of evidence from unbiased single-cell molecular profiling and functional dissection of the BMSC lineage hierarchy using in vivo cell lineage analysis. (nature.com)

This study focuses to identify, isolate and characterize the mesenchymal stem cells that are believed to be present in BM. (journalcra.com)

Stem cells translational medicine , 8 (3), 271-284. (birmingham.ac.uk)

How to Harvest Bone Marrow Derived Mesenchymal Stem Cells for Expansion and Injection. (ivis.org)
The intracavernous (i.c.) injection of stem cells (SCs) has been shown to improve erectile function in various erectile dysfunction (ED) animal models. (stemcellclinicsofamerica.net)
At 2 and 7 days post injection, penises and femoral bone marrow were processed for histological analyses. (stemcellclinicsofamerica.net)

The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)

The miR mimics or inhibitor was transfected into laryngocarcinoma cell lines M4E and Hep2 for the investigation of the biological functions (proliferative, invasion, migratory rates, and apoptotic rates) of this miRNA. (hindawi.com)
MiR mimics could inhibit proliferative, invasive migratory rates while promoting apoptotic rates of M4E and Hep2 cells. (hindawi.com)
For example, miR-552 promotes cell proliferative rate of laryngocarcinoma cells by modulating p53 signaling [ 5 ]. (hindawi.com)
MiR-125b participates in the oncogenic role of lncRNA MALAT1 in the phenotypes including cell proliferative and invasive rates in laryngocarcinoma [ 6 ]. (hindawi.com)
Recently, few studies revealed that miR-29a-3p could suppress the proliferative and invasive rates in laryngocarcinoma cells [ 10 , 11 ]. (hindawi.com)
however, the level of telomerase activity is low or absent in the majority of stem cells regardless of their proliferative capacity. (nature.com)
Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
As stem cells have elongated proliferative capacity, they should have a mechanism that maintains telomere length through many cell divisions. (nature.com)

Positive for CD29, CD44, CD73, CD90, CD105, and CD166 (greater than 95% of the cell population expresses these markers by flow cytometry). (ubpone.com)
Negative for CD14, CD31, CD34, and CD45 (less than 2% of cell population expresses these markers by flow cytometry). (ubpone.com)
Cells are characterized by flow cytometry for CD90/CD105/CD29/CD45/CD34 and are negative for mycoplasma/yeast/fungi/bacteria. (mbpinc.net)
Whole femoral bone marrows were also analyzed for EdU-positive cells by flow cytometry. (stemcellclinicsofamerica.net)

This simple pellet-based 3D culture system allows for cell aggregation and spheroid formation, facilitating cell-cell and cell-extracellular matrix interactions that better mimic the in vivo cellular environment compared with 2D monolayer cultures. (lu.se)
Use of PriCoat TM T25 Flasks ( G299 ) or Applied Cell Extracellular Matrix ( G422 ) is required for cell adhesion to the culture vessels. (mbpinc.net)

Blood cells, molecules & diseases 2022 8 97 102698. (cdc.gov)

Therefore, Fgfr3 + endosteal SSCs are abundant in young bone marrow and provide a robust source of osteoblasts, contributing to both normal and aberrant osteogenesis. (nature.com)
Of note, we define OCT identities as a state with some characteristics of both osteoblasts and chondrocytes, instead of cell-type plasticity between osteoblasts and chondrocytes. (nature.com)

that is, they have the ability to generate other stem cells and perpetuate themselves. (bvsalud.org)

Multiplacenta derived stem cell/cytokine treatment increases survival time in a mouse model with radiation-induced bone marrow damage. (stembook.org)
However it is important to note that the viability of some sensitive cell types may be reduced by the temperature shift of such treatment, making recovery more difficult. (mbpinc.net)
Critically, we show that sequential treatment with the mitogens epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2) followed by postnatal hippocampal astrocyte conditioned medium significantly promotes the generation of neurofilament(+)/beta-tubulin(+) cells from bone marrow precursors. (ox.ac.uk)
Expression profile of microRNAs following bone marrow‑derived mesenchymal stem cell treatment in lipopolysaccharide‑induced acute lung injury. (logosbio.com.cn)
Stem cells have potential as a source of cells and tissues for research and treatment of disease. (tocris.com)
Hemin (plasma-derived intravenous heme) is the definitive treatment and mainstay of management. (medscape.com)

The multifunctional nuclear protein large T-antigen was detectable by immunohistochemical analyses in a subset of neoplastic cells. (cdc.gov)

Stem cells are undifferentiated cells that have the potential to become one of 200 types of cells in the body, including blood, nerve, muscle, heart, glandular, and skin cells. (msdmanuals.com)

Thus, although PyV-induced oncogenesis in laboratory animals has been a prolific model for the study of the cell cycle and cell transformation, natural infections rarely result in tumor formation, so the steps in cell transformation after natural infection are being revealed more slowly. (cdc.gov)
1) measurement of a sample of subjects for bone lead to determine the correlation of the blood lead measurements with cumulative exposure as measured by bone lead, and 2) assessment of whether Helicobacter pylori infection has been more common among those with higher blood leads. (who.int)

In detail, thymus-settling progenitors enter the thymus and generate early T-cell progeni- tors (ETP/DN1 stage), which in turn give rise to DN2, DN3 and DN4 cells. (lu.se)

Anatomy55

Organisms14

Diseases33

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment58

Phenomena and Processes50

Disciplines and Occupations3

Technology, Industry, Agriculture3

Information Science3

Named Groups1

Health Care5

MSCs45

Progenitor cells6

Stromal10

Endothelial cells2

BMSCs17

Hematopoietic Stem1

Osteogenic differentiation2

Diseased tissues1

Adipose tissue-derived1

Tissue12

Vitro5

Mononuclear cells1

Transplantation7

Therapy4

Allogeneic2

Umbilical2

Exosomal3

Adipocytes2

Proliferation5

Pluripotent stem1

Adult bone1

Cartilage1

Astrocytes1

Differentiate3

Osteoblast2

Therapies6

Regeneration3

Populations2

Embryonic2

Human15

Somatic5

Clinical8

Therapeutic5

ADSCs3

Aspirate3

BMSC1

Isolate1

Translational medicine1

Injection3

Neurons2

Proliferative8

Flow cytometry4

Extracellular Matrix2

Diseases1

Osteoblasts2

Ability to generate1

Treatment6

Analyses1

Potential1

Infection2

Generate1