• In this study, we investigated the modulation of pDC differentiation in Flt-3 ligand (Flt3L)-supplemented BM cultures, using highly purified mesenchymal stem cells (MSCs) that were FACS-isolated from murine BM based on surface marker expression and used after in vitro expansion. (nih.gov)
  • Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. (nature.com)
  • Mesenchymal stem cells (MSCs) are multipotent stem cells with a high capacity for self-renewal. (mbpinc.net)
  • In addition, bone marrow (BM)-derived mesenchymal stem cells (MSCs) have been suggested as an effective therapy for hepatic fibrosis and cirrhosis. (elsevierpure.com)
  • The aim of this study was to determine whether the combination treatment of simvastatin plus MSCs (Sim-MSCs) could have a synergistic effect on hepatic fibrosis in a thioacetamide (TAA)-induced cirrhotic rat model and hepatic stellate cells (HSCs). (elsevierpure.com)
  • Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. (ijbs.com)
  • In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differentiate into various mesodermal lineages including osteoblast/osteocyte, chondrocyte, and adipocyte that are crucial for bone metabolism. (ijbs.com)
  • While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. (ijbs.com)
  • To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. (ijbs.com)
  • We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. (ijbs.com)
  • We describe a novel therapeutic approach for cirrhosis using mesenchymal stem cells (MSCs) and colony‐stimulating factor‐1‐induced bone marrow‐derived macrophages (id‐BMMs) and analyze the mechanisms underlying fibrosis improvement and regeneration. (birmingham.ac.uk)
  • Mouse MSCs and id‐BMMs were cultured from mouse bone marrow and their interactions analyzed in vitro. (birmingham.ac.uk)
  • Intravital imaging showed that after combination cell administration, a large number of id‐BMMs, which phagocytosed hepatocyte debris and were retained in the liver for more than 7 days, along with a few MSCs, the majority of which were trapped in the lung, migrated to the fibrotic area in the liver. (birmingham.ac.uk)
  • Indirect effector MSCs and direct effector id‐BMMs synergistically improved cirrhosis along with host cells in mice. (birmingham.ac.uk)
  • Mesenchymal stem cells (MSCs) have recently been shown to home to tumors and contribute to the formation of the tumor-associated stroma. (oncotarget.com)
  • In this study, we report that interleukin-8 (IL-8) was the most highly upregulated pro-angiogenic factor in MSCs co-cultured with CRC cells and was expressed at substantially higher levels in MSCs than CRC cells. (oncotarget.com)
  • To evaluate the effect of MSC-derived IL-8 on CRC angiogenesis and growth, we used MSCs that expressed small hairpin (interfering) RNAs (shRNA) targeting IL-8 (shIL-8-MSCs). (oncotarget.com)
  • The key attributes like isolation efficiency, cell yield, colony-forming unit-fibroblast (CFU-F) frequency, phenotypic characteristics, and multi-lineage differentiation capacity were determined for the isolated MSCs from bone marrow specimens. (journalcra.com)
  • Further, when those MSCs were analyzed for their specific marker expression using flowcytometry, they were found to be positive for CD29, CD73, CD90, and CD105 and negative for CD34 and CD45 stem cell markers respectively. (journalcra.com)
  • Apart from this, it was observed that those MSCs, when induced to differentiate into cardiomyocytes, they successfully differentiated into cardiomyocyte like cells with flat, wide, myotube like structure with sarcomeric organization, which were later confirmed to be cardiomyocytes by qRT-PCR. (journalcra.com)
  • Thus, this study shows that MSCs were isolated from human bone-marrow and subsequently differentiated into cardiomyocytes with 100% success rate. (journalcra.com)
  • Introduction Mesenchymal stem cells (MSCs) are essential members from the stem cells family members [1]. (researchassistantresume.com)
  • MSCs are ideal stem cells for tissues regeneration because of their excellent capacities for differentiation and proliferation. (researchassistantresume.com)
  • Administration of bone tissue marrow-derived mesenchymal stem cells (MSCs) can be an innovative strategy for the treating a variety of diseases that aren't curable by current therapies including center failure. (bio-biz-navi.com)
  • This review content summarizes protocols to isolate and broaden bone tissue marrow-derived MSCs in 47 latest scientific studies of MSC-based therapy that have been released after 2007 onwards and supplied sufficient methodological details. (bio-biz-navi.com)
  • 1 Launch Recent research provides extensively proven the potential of bone tissue marrow- (BM-) produced mesenchymal stem cells (MSCs) for regenerative remedies in a variety of organs like the center [1]. (bio-biz-navi.com)
  • Because the initial scientific trial of BMC shot in 1995 [3] a lot more than 2 0 sufferers have been implemented with allogeneic or autologous MSCs for the treating various illnesses including graft-versus-host disease hematologic malignancies cardiovascular illnesses neurologic illnesses autoimmune diseases body organ transplantation refractory wounds and bone tissue/cartilage flaws [4]. (bio-biz-navi.com)
  • MSCs stand for 0.001-0.01% of BM mononuclear cells or lower [8]. (bio-biz-navi.com)
  • Studies on therapeutic administration of bone marrow-derived mesenchymal stem cells (MSCs) in ALI caused by the viral infection have been limited in number and have shown conflicting results. (unair.ac.id)
  • Methods: MSCs were prepared from the bone marrow of 9 to 12 week-old BALB/c mice. (unair.ac.id)
  • Conclusion: The administration of MSCs prevented further lung injury and inflammation, and enhanced alveolar cell type II and I regeneration, while it did not significantly affect viral proliferation and mouse morbidity and mortality. (unair.ac.id)
  • Recent findings suggest that bone marrow-derived mesenchymal stem cells (BM-MSCs) are recruited into the microenvironment of developing tumors, where they contribute to metastatic processes. (houstonmethodist.org)
  • The aim of this study was to investigate the role of BM-MSCs in promoting osteosarcoma and hepatocellular carcinoma cell progression in vitro and the possible mechanisms involved in these processes. (houstonmethodist.org)
  • U2OS and SNU-398 are osteosarcoma and hepatocellular carcinoma cell lines, respectively, that can be induced to proliferate when cultured in the presence of BM-MSCs. (houstonmethodist.org)
  • To determine the effect of BM-MSCs on U2OS and SNU-398 cells, the AKT and ERK signaling pathways were investigated, and increases were observed in active P-Akt and P-Erk forms. (houstonmethodist.org)
  • Moreover, BM-MSCs caused an increase in tumor cell migration and invasion that was derived from the enhancement of CXCR4 levels.Thus, when tumor cells were treated with the CXCR4 antagonist AMD3100, a reduction in their migration and invasion was observed. (houstonmethodist.org)
  • Taken together, these results suggest that inhibiting CXCR4 impairs the cross-talk between tumor cells and BM-MSCs, resulting in reduced metastatic potential in osteosarcoma and hepatocellular carcinoma cells. (houstonmethodist.org)
  • Recently, mesenchymal stem cells (MSCs) have been suggested for use in the cell-based treatment of cartilage lesions. (researchgate.net)
  • These correlations were not observed in group B. Conclusion: Injection of MSCs with marrow stimulation treatment was encouraging in patients older than 50 years compared with patients treated with marrow stimulation treatment alone, especially when the lesion size was larger than 109 mm(2) or a subchondral cyst existed. (researchgate.net)
  • In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
  • MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
  • Mesenchymal Stem Cells (MSCs) are a heterogeneous subset of stromal stem cells that originate from the mesoderm layer of the fetus. (tocris.com)
  • Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
  • In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
  • Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
  • Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
  • The study also showed that the adult OCRs are distinct from mesenchymal stem cells (MSCs), which play a role in bone generation during development and adulthood. (sciencedaily.com)
  • Researchers presumed that MSCs were the origin of all bone, cartilage, and fat, but recent studies have shown that these cells do not generate young bone and cartilage. (sciencedaily.com)
  • The CUMC study suggests that OCR stem cells actually fill this function and that both OCR stems cells and MSCs contribute to bone maintenance and repair in adults. (sciencedaily.com)
  • We found that myocilin is expressed in bone marrow-derived mesenchymal stem cells (MSCs) and affects their differentiation into osteoblasts. (nih.gov)
  • To evaluate the number of adverse events or severe adverse event assessed by Common Terminology Criteria For Adverse Events (CTCAE) of intravenous infusion of human allogeneic BM-derived MSCs product StromaForte for all study participants. (who.int)
  • Additionally, it will explain the techniques of collecting, packaging, and transporting bone marrow aspirates for collection, expansion, and 150 injections into diseased tissues. (ivis.org)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers. (lu.se)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers significant implications for the study of cartilage biology and the discovery of potential therapeutic interventions for cartilage-related and associated disorders. (lu.se)
  • Bone marrow mesenchymal stem cells (BMSCs) have been reported to attenuate myocardial I/R injury via their paracrine effects, which can be enhanced by hypoxic preconditioning. (researchsquare.com)
  • We hypothesized that the donor heart preservation with hypoxic conditioned medium derived from BMSCs (CM-BMSCs) would improve post-transplant graft function. (researchsquare.com)
  • The administration of bone marrow-derived mesenchymal stem cells (BMSCs) is emerging as a promising approach for I/R injury-induced myocardial infarction due to its multiple differentiation potential(7, 8). (researchsquare.com)
  • Bone marrow mesenchymal stem cells (BMSCs) have potential therapeutic benefits for the treatment of endometrial diseases and injury. (biologists.com)
  • BMSCs interact with uterus parenchymal cells by direct contact or indirect secretion of growth factors to promote functional recovery. (biologists.com)
  • Accordingly, investigations on cellular therapies have therefore moved to progenitor cell populations such as bone marrow derived mesenchymal stem cells (BMSCs), which have the ability to differentiate into cartilage cells 4 . (nature.com)
  • Mouse bone marrow -derived mesenchymal stem cells (BMSCs) were selected and BMSC- exosomes (BMSC-EXO) were extracted and then identified. (bvsalud.org)
  • Bone tissue marrow-derived mesenchymal stem cells (BMSCs) are widely used in regenerative medicine in light of their ability to differentiate along the chondrogenic and osteogenic lineages. (researchassistantresume.com)
  • Bone marrow-derived mesenchymal stem/stromal cells (BMSCs) are fundamental to bone regenerative therapies, tissue engineering, and postmenopausal osteoporosis. (elsevierpure.com)
  • Donor variation among patients, cell heterogeneity, and unpredictable capacity for differentiation reduce effectiveness of BMSCs for regenerative cell therapies. (elsevierpure.com)
  • The cell surface glycoprotein CD24 exhibits the most prominent differential expression during osteogenic versus adipogenic differentiation of human BMSCs. (elsevierpure.com)
  • In undifferentiated human BMSCs, CD24 cell surface expression is variable among donors (range: 2%-10%) and increased by two to fourfold upon osteogenic differentiation. (elsevierpure.com)
  • RNAseq analysis of CD24pos and CD24neg BMSCs identified a limited number of genes with increased expression in CD24pos cells that are associated with cell adhesion, motility, and extracellular matrix. (elsevierpure.com)
  • Hence, expression of the cell surface glycoprotein CD24 identifies a subpopulation of human BMSCs with reduced capacity for proliferation and extracellular matrix mineralization. (elsevierpure.com)
  • Functional specialization among BMSCs populations may support their regenerative potential and therapeutic success by accommodating cell activities that promote skeletal tissue formation, homeostasis, and repair. (elsevierpure.com)
  • Bone marrow-derived mesenchymal stem cells (BMSCs) have been reported to have a potential therapeutic role on SAP, but the specific mechanism is unclear. (drcalapai.com)
  • The capacities of proliferation, migration, and repair of human umbilical vein endothelial cells were enhanced by BMSCs supernatant. (drcalapai.com)
  • BMSCs-derived exosomal miR-138-5p reduces neuron injury following IS in vivo. (biomedcentral.com)
  • However, there have been some cases where there were both improvement and toxicity inflicted on the targeted organ, as well as cases where treatment of mesenchymal stem cells did not show improvement of function at all. (wikipedia.org)
  • The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
  • MiR-29a-3p was enriched in hBMSC-derived Exo, and the Exo from miR-29a-3p mimics transfected hBMSCs could inhibit laryngocarcinoma cell malignant phenotypes in vitro and prevent tumor progression in vivo . (hindawi.com)
  • Currently, the only FDA-approved cell-based therapy for cartilage defects involves autologous chondrocyte implantation: chondrocytes harvested from low-contact areas are expanded in vitro and then re-injected directly into the damaged site 1 . (nature.com)
  • These studies have demonstrated that mechanical forces are able to shape the mesenchymal stem cell (MSC) fate when appropriately applied in in vitro models of cartilage regeneration. (nature.com)
  • A needle is inserted into the back of the patients hip bone and cells are removed to be grown under controlled in vitro conditions in a lab. (wikipedia.org)
  • The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
  • In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
  • Korpershoek JV, Rikkers M, Wallis FSA, Dijkstra K, Te Raa M, de Knijff P, Saris DBF , Vonk LA. Mitochondrial Transport from Mesenchymal Stromal Cells to Chondrocytes Increases DNA Content and Proteoglycan Deposition In Vitro in 3D Cultures. (mayo.edu)
  • Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
  • This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
  • Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
  • Background: Chondrocytes are the primary cells responsible for maintaining cartilage integrity and function. (lu.se)
  • Besides the cell source choice, also mechanical stimuli are crucial in the development and maintenance of articular cartilage. (nature.com)
  • A stem cell capable of regenerating both bone and cartilage has been identified in bone marrow of mice. (sciencedaily.com)
  • Using this marker, the researchers found that OCR cells self-renew and generate key bone and cartilage cells, including osteoblasts and chondrocytes. (sciencedaily.com)
  • If you make a fracture in the mouse, these cells will come alive again, generate both bone and cartilage in the mouse--and repair the fracture. (sciencedaily.com)
  • Osteoarthritis is a chronic arthropathy characterized by disruption and potential loss of joint cartilage along with other joint changes, including bone hypertrophy (osteophyte formation). (msdmanuals.com)
  • Obesity triggers some of these defects in cartilage metabolism, leading to cartilage matrix damage and subchondral bone remodel-ing mediated by adipokines, such as leptin and adipsin, and compounded by mechanical factors due to excess weight. (msdmanuals.com)
  • We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
  • It is essential that the cells be subculture BEFORE reaching confluence as post-confluent cells exhibit changes in morphology, slower proliferation, and reduced differentiation after passaging. (ubpone.com)
  • We found that MSC-secreted IL-8 promoted human umbilical vein endothelial cell (HUVEC) proliferation and migration, tube-formation ability and CRC cell proliferation. (oncotarget.com)
  • The results showed that quercetin significantly enhanced the cell proliferation osteogenic differentiation and angiogenic factor secretion of rBMSCs in a dose-dependent manner with a concentration of 2 μM achieving the best stimulatory effect. (researchassistantresume.com)
  • Downregulated genes are associated with cell cycle regulation, and biological assays revealed that CD24pos cells have reduced proliferation. (elsevierpure.com)
  • Bone marrow-derived hematopoietic and mesenchymal stem cells as targets for Histoplasma capulatum infection: implications for hematopoiesis, differentiation, and proliferation. (urosario.edu.co)
  • To determine whether the interaction of hematopoietic stem cells (HSCs) and bone marrow-derived mesenchymal stem cells (BMMSCs) with Histoplasma capsulatum affects hematopoiesis, differentiation and proliferation of these precursor cells. (urosario.edu.co)
  • BMMSCs) with Histoplasma capsulatum affects hematopoiesis, differentiation and proliferation of these precursor cells. (urosario.edu.co)
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • We discovered that myocilin increased cell proliferation and survival. (nih.gov)
  • liver cells and endothelial cells. (mbpinc.net)
  • As demonstrated in previous studies many approaches have been applied to stimulate angiogenesis in the process of bone healing including the combination with endothelial cells the application of vascular growth factors vascular redesigning by microsurgery and gene transfection techniques [14-17]. (researchassistantresume.com)
  • In conclusion, hBMSC-derived Exo with upregulated miR-29a-3p inhibited laryngocarcinoma progression via regulating PTEN, providing a potential diagnostic and therapeutic target in this disease. (hindawi.com)
  • With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
  • In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
  • Recent evidence suggests that pharmacological treatment devoted to regulating stem cell function is a potential new therapeutic strategy that is drawing nearer to clinical practice. (elsevierpure.com)
  • In the research process of expanding the therapeutic uses of mesenchymal stem cells, they are grown in laboratories or grown using medication to stimulate new cell growth within the human body. (wikipedia.org)
  • The work also suggests a much more efficient way of differentiating stem cells for therapeutic purposes. (sciencedaily.com)
  • Interestingly Chinese medicine which could promote the secretion of osteogenic factors such as collagen type 1 (COL1) bone morphogenetic protein 2 (BMP-2) osteocalcin (OCN) and osteopontin (OPN) and the gene manifestation of BMP-4 runt-related transcription element 2 (Runx2) OCN and OPN has been increasingly applied in osteogenic induction study [18-20]. (researchassistantresume.com)
  • The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. (scielo.br)
  • On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)
  • The findings also help explain why the process of differentiating stem cells into specific lineages in the laboratory has been highly inefficient. (sciencedaily.com)
  • At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
  • Previous studies indicated that exosomal miR-29a-3p derived from human bone mesenchymal stem cells (hBMSCs) could efficiently suppress cell migrative ability and vasculogenic mimicry in glioma [ 15 ]. (hindawi.com)
  • Due to its role in laryngocarcinoma, we speculated that the exosomal miR-29a-3p derived from hBMSCs might also exert a potential impact in cancer progression. (hindawi.com)
  • Mouse bone marrow derived mesenchymal stem cells-secreted exosomal microRNA-125b-5p suppresses atherosclerotic plaque formation via inhibiting Map4k4. (bvsalud.org)
  • These bone marrow-derived mesenchymal stem cells were isolated from a young adult male Fischer rat leg bone. (mbpinc.net)
  • Adult human bone marrow (BM) is the major source of mesenchymal stem cells (MSC) for cell-based therapies. (journalcra.com)
  • Adult mesenchymal stem cells are being used by researchers in the fields of regenerative medicine and tissue engineering to artificially reconstruct human tissue which has been previously damaged. (wikipedia.org)
  • In mesenchymal stem cell therapy, most of the cells are extracted from the adult patient's bone marrow Mesenchymal stem cells can be obtained via a procedure called bone marrow aspiration. (wikipedia.org)
  • In an adult, roughly half of the bone marrow is still red. (medscape.com)
  • Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)
  • 2001. Subchronic dermal application of N,N-diethyl m-toluamide (DEET) and permethrin to adult rats, alone or in combination, causes diffuse neuronal cell death and cytoskeletal abnormalities in the cerebral cortex and the hippocampus, and purkinje neuron loss in the cerebellum. (cdc.gov)
  • Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
  • They can develop into specialized cells of the body such as chondrocytes/osteoblasts/adipocytes. (mbpinc.net)
  • The researchers believe that OCR stem cells will be found in human bone tissue, as mice and humans have similar bone biology. (sciencedaily.com)
  • Led by Sui Huang, MD, PhD, a Visiting Associate Professor in the Children's Hospital Boston Vascular Biology Program (now also on the faculty of the University of Calgary), and Hannah Chang, an MD/PhD student in Children's Vascular Biology Program, the researchers examined how blood stem cells "decide" to become white blood cell progenitors or red blood cell progenitors. (sciencedaily.com)
  • Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
  • However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
  • Mesenchymal stem cells are able to differentiate, or mature from a less specialized cell to a more specialized cell type, to replace damaged tissues in various organs. (wikipedia.org)
  • Over a course of two or three weeks, the cells will multiply and differentiate into specialized cells. (wikipedia.org)
  • During long-term culture, mesenchymal stem cells age, lose their ability to differentiate, and have a higher chance to undergo malignant transformation. (wikipedia.org)
  • They can differentiate into many cell types including bone, fat, and muscle which allow them to treat a large range of disorders. (wikipedia.org)
  • They can differentiate into cells of the mesodermal lineage, such as adipocytes, and osteocytes. (tocris.com)
  • The conventional view of how stem cells differentiate assumes that cells are "instructed" to progress along prescribed signaling pathways. (sciencedaily.com)
  • It reveals an elegant yet simple system that maintains cells in a stable state, yet enables them to differentiate under the right conditions. (sciencedaily.com)
  • Instead, it supports the idea that cells differentiate through the collective behavior of multiple genes in a network that ultimately leads to just a few endpoints -- just as a marble on a hilltop can travel a nearly infinite number of downward paths, only to arrive in the same valley. (sciencedaily.com)
  • But most important, the differences in Sca-1, GATA1 and PU.1 levels across the three cell groups became less pronounced over time, as did the variability in the cells' propensity to differentiate, suggesting that the differences are transient. (sciencedaily.com)
  • Together, the findings make the case that a slow fluctuation or cycling of gene activity tends to maintain cells in a stable state, while also priming them to differentiate when conditions are right. (sciencedaily.com)
  • As needed, the stem cells differentiate to become a particular kind of cell-a white blood cell, red blood cell, or platelet. (medscape.com)
  • T lymphocytes originate via lymphoid stem cells that migrate to the thymus and differentiate under the influence of the thymic hormones thymopoietin and thymosin. (medscape.com)
  • Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • A critical length of telomere repeats is required to ensure proper telomere function and avoid the activation of DNA damage pathways that result in replicative senescence or cell death. (nature.com)
  • Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. (tocris.com)
  • A new study suggests that the conventional view, which assumes that cells are "instructed" to progress along prescribed signaling pathways, is too simplistic. (sciencedaily.com)
  • The exosomes (Exo) from human bone marrow mesenchymal stem cells (hBMSCs) after the transfection of miR mimics/inhibitor/si-PTEN were isolated and used to stimulate M4E and Hep2 cells. (hindawi.com)
  • In this study, exosomes from bone marrow plasma were successfully extracted and identified. (techscience.com)
  • Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
  • The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
  • This may result in clinicians opting out of pursuing this choice of stem cell therapy. (ivis.org)
  • Host macrophages and neutrophils infiltrated after combination therapy and contributed to liver fibrosis regression and promoted regeneration along with administered cells. (birmingham.ac.uk)
  • Human mesenchymal stem cell therapy is limited due to variation in individual response to treatment and the high number of cells needed for treatment. (wikipedia.org)
  • Our guide highlights the use of small molecules in stem cell research and cell therapy and lists relevant products. (tocris.com)
  • Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)
  • Human MSC are easy to isolate from small aspirate of bone marrow via their adherence ability. (biomedcentral.com)
  • Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
  • GENERAL INFORMATION: GFP HBMMSCs (UBP-0050) are isolated from human Bone Marrow tissues and demonstrated with spindle-shaped-, and fibroblast-like cells. (ubpone.com)
  • In the present study, six samples of human bone-marrow were collected. (journalcra.com)
  • And hence, this study suggests that human bone marrow is a potential source of mesenchymal stem cells that could be differentiated in to ccardiomyocytes and used for cell based therapies for treating various cardiovascular diseases. (journalcra.com)
  • We hypothesized that human mesenchymal stem cells seeded onto poly(1,8-octanediol-co-citrate) elastomeric thin films would provide a suitable milieu for partial bladder regeneration. (northwestern.edu)
  • Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
  • The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
  • Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
  • Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
  • In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
  • Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
  • In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
  • PyV-induced tumorigenesis in laboratory animals, by simian virus 40 (SV40) or by multiple human PyVs, such as JC virus (JCV), is used extensively as a cell transformation model. (cdc.gov)
  • A Longitudinal Open-Label Multi-center Single-Arm Phase I/IIa Study to Evaluate the Safety and Preventive Effect on Cardiovascular Events of Human Allogenic Bone-Marrow-Derived Mesenchymal Stromal Cell Product, StromaForte, in Study Participants. (who.int)
  • These protective effects may be associated with the inhibition of apoptosis and inflammation, as reflected by less TUNEL-positive cells and lower levels of serum proinflammatory cytokines (Interleukin-1β, Interleukin-6, tumor necrosis factor-α) and cardiac troponin I in the N-CM group compared with the vehicle group. (researchsquare.com)
  • However, the involvement of MSC-derived cytokines in colorectal cancer (CRC) angiogenesis and growth has not been clearly addressed. (oncotarget.com)
  • The regeneration of bone tissue defects specifically includes a series of complicated procedures that are controlled by a number of cytokines and natural signals. (researchassistantresume.com)
  • The miR mimics or inhibitor was transfected into laryngocarcinoma cell lines M4E and Hep2 for the investigation of the biological functions (proliferative, invasion, migratory rates, and apoptotic rates) of this miRNA. (hindawi.com)
  • MiR mimics could inhibit proliferative, invasive migratory rates while promoting apoptotic rates of M4E and Hep2 cells. (hindawi.com)
  • For example, miR-552 promotes cell proliferative rate of laryngocarcinoma cells by modulating p53 signaling [ 5 ]. (hindawi.com)
  • MiR-125b participates in the oncogenic role of lncRNA MALAT1 in the phenotypes including cell proliferative and invasive rates in laryngocarcinoma [ 6 ]. (hindawi.com)
  • Recently, few studies revealed that miR-29a-3p could suppress the proliferative and invasive rates in laryngocarcinoma cells [ 10 , 11 ]. (hindawi.com)
  • however, the level of telomerase activity is low or absent in the majority of stem cells regardless of their proliferative capacity. (nature.com)
  • Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
  • As stem cells have elongated proliferative capacity, they should have a mechanism that maintains telomere length through many cell divisions. (nature.com)
  • and NF-?B signaling play a critical role in cigarette smoke-induced epithelial-mesenchymal transition of retinal pigment epithelial cells in proliferative vitreoretinopathy. (rochester.edu)
  • How to Harvest Bone Marrow Derived Mesenchymal Stem Cells for Expansion and Injection. (ivis.org)
  • Mesenchymal stem cells have also been shown to travel to sites of inflammation far from the injection site. (wikipedia.org)
  • Purpose: To compare the clinical outcomes of MSC injection and arthroscopic marrow stimulation treatment with those of arthroscopic marrow stimulation treatment alone for the treatment of OLTs in older patients. (researchgate.net)
  • Patients were divided into 2 groups: 35 patients (37 ankles) treated with arthroscopic marrow stimulation treatment alone (group A) and 30 patients (31 ankles) who underwent MSC injection along with arthroscopic marrow stimulation treatment (group B). Clinical outcomes were evaluated according to the visual analog scale (VAS) for pain, the American Orthopaedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot Scale, and the Roles and Maudsley score. (researchgate.net)
  • Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia. (physiciansweekly.com)
  • The lack of any hydrogel-derived immunoreaction was demonstrated in vivo by mice subcutaneous implantation. (nature.com)
  • The pathophysiological characteristics of AD depend on the location and severity of neuropathology, which is derived from the comprehensive effect of different mechanisms such as autophagy, oxidative stress, apoptosis, inflammation, and immunoregulation. (biomedcentral.com)
  • The findings, published in the May 22 issue of Nature, give a glimpse into how that collective behavior works, and show that cell populations maintain a built-in variability that nature can harness for change under the right conditions. (sciencedaily.com)
  • They began by examining populations of seemingly identical blood stem cells, and found that a cell marker of "stemness," a protein called Sca-1, was actually present in highly variable amounts from cell to cell -- in fact, they found a 1,000-fold range. (sciencedaily.com)
  • This study focuses to identify, isolate and characterize the mesenchymal stem cells that are believed to be present in BM. (journalcra.com)
  • Expression profile of microRNAs following bone marrow‑derived mesenchymal stem cell treatment in lipopolysaccharide‑induced acute lung injury. (logosbio.com.cn)
  • Although our approach has several advantages, further investigation is required to address its limitations, such as the potential differences between chondrocytes generated using our protocol and those derived from other established methods, and to refine the model for broader applicability and clinical translation. (lu.se)
  • However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. (biomedcentral.com)
  • Their easy accessibility as well as exceptional genomic stability and their capacity for tissue repair with few immunological and ethical issues make them the favorable cell type for experimental and clinical use. (mbpinc.net)
  • In previous studies which observed the safety of clinical mesenchymal stem cell use, no serious side effects were noted. (wikipedia.org)
  • 3. Have a clinical history of malignancy within 2.5 years (i.e., study participants with prior malignancy must be cancer free for 2.5 years) except curatively treated basal cell carcinoma, melanoma in situ, or cervical carcinoma. (who.int)
  • One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
  • Fibrosis regression, liver regeneration, and liver‐migrating host cells were evaluated. (birmingham.ac.uk)
  • since bone tissue regeneration cannot effectively occur with out a bloodstream supply achieving the bone tissue defect angiogenesis CEP-28122 is certainly important for bone tissue regeneration in vivo [6-10]. (researchassistantresume.com)
  • It really is reported that angiogenesis may be the foundational part of bone tissue regeneration particularly in calvarial and limb bone tissue defects [11-13]. (researchassistantresume.com)
  • after that both angiogenesis and osteogenesis participate in the bone regeneration and promote the effects of each additional [13]. (researchassistantresume.com)
  • Bladder regeneration studies have yielded inconclusive results possibly due to the use of unfavorable cells and primitive scaffold design. (northwestern.edu)
  • Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
  • Each lot of UBP-0050 is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM), UBP-35), when cultured following the detailed protocol described below). (ubpone.com)
  • Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. (tocris.com)
  • Our Stem Cells review gives an overview of the use of small molecules in the control of stem cell growth & differentiation and somatic cell reprogramming. (tocris.com)
  • Blood stem cells with low levels of Sca-1 differentiated into red blood cell progenitors seven times more often than cells high in Sca-1 when exposed to erythropoietin, a growth factor that promotes red blood cell production. (sciencedaily.com)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
  • Bone marrow supersedes the liver as the major hematopoietic organ at 32-36 weeks' gestation. (medscape.com)
  • Also, transplanted mesenchymal stem cells pose little risk for rejection as they are derived from the patients own tissue, so are genetically identical, however graft versus host disease is a possibility, where the cells change enough while outside the patient's body that the immune system recognizes them as foreign and can attempt to reject them. (wikipedia.org)
  • However, for cell therapies MSC have to be expanded and/or manipulated to obtain a sufficient amount of cells that can be subsequently used for treatment. (biomedcentral.com)
  • Our findings raise the possibility that drugs or other therapies can be developed to stimulate the production of OCR stem cells and improve the body's ability to repair bone injury--a process that declines significantly in old age," says Timothy C. Wang, MD, the Dorothy L. and Daniel H. Silberberg Professor of Medicine at CUMC, who initiated this research. (sciencedaily.com)
  • This simple pellet-based 3D culture system allows for cell aggregation and spheroid formation, facilitating cell-cell and cell-extracellular matrix interactions that better mimic the in vivo cellular environment compared with 2D monolayer cultures. (lu.se)
  • In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
  • Chondrocytes have the longest cell cycle in the body (similar to central nervous system and muscle cells). (msdmanuals.com)
  • The mature cells are then harvested and injected back into the patient through local delivery or systemic infusion. (wikipedia.org)
  • Conversely, when stem cells were exposed to granulocyte--macrophage colony-stimulating factor, which stimulates white blood cell formation, those that were highest in Sca-1 were the most likely to become white cells. (sciencedaily.com)
  • When the oxygen content of body tissues is low, if there is loss of blood or anemia, or if the number of red blood cells decreases, the kidneys produce and release erythropoietin, a hormone that stimulates the bone marrow to produce more red blood cells. (medscape.com)
  • There is a signal crosstalk between autophagy and apoptosis, which may be regulated to produce synergistic effect on the preconditioning of stem cells. (biomedcentral.com)
  • Oppositely, expression of mutated myocilin sensitizes cells to apoptosis induced by oxidative stress. (nih.gov)
  • Progenitor cell (stem cell) lines in the bone marrow produce new blood cells and stromal cells. (medscape.com)
  • We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)