• Functional characterization of the horizontally transferred 4,5-DOPA extradiol dioxygenase gene in the domestic silkworm, Bombyx mori. (cqu.edu.cn)
  • Comparative analysis of iTRAQ-based proteomes for cocoons between the domestic silkworm (Bombyx mori) and wild silkworm (Bombyx mandarina). (cqu.edu.cn)
  • Identification of two isoforms of Pop in the domestic silkworm, Bombyx mori: Cloning, characterization and expression analysis. (cqu.edu.cn)
  • Transcription factor E74A affects the ecdysone titer by regulating the expression of the EO gene in the silkworm, Bombyx mori. (cqu.edu.cn)
  • Additional studies have also shown that hemolymph from the silkworm ( Bombyx mori ) can inhibit insect and mammalian cell apoptosis that is induced by viruses and several chemical inducers, such as staurosporine, camptothecin, and actinomycin D [ 6 - 8 ]. (hindawi.com)
  • A. M. Khurad, A. Mahulikar, M. K. Rathod, M. M. Rai, S. Kanginakudru, Nagaraju J. (2004) Vertical transmission of nu-cleopolyhedrovirus in the silkworm, Bombyx mori L. Journal of Invertebrate Pathology 87, 8-15. (scirp.org)
  • Screening and Identification of Candidate Proteins Interacting with BmHSP60 in the Silkworm ( Bombyx mori )[J]. Scientia Agricultura Sinica, 2019, 52(2): 376-384. (chinaagrisci.com)
  • Asano T., Ashida M. (2001) Cuticular pro-phenoloxidase of the silkworm, Bombyx mori . (apidologie.org)
  • Nakato H., Toriyama M., Izumi S., Tomino S. (1990) Structure and expression of mRNA for a pupal cuticle protein of the silkworm, Bombyx mori , Insect Biochem. (apidologie.org)
  • Izumi S., Tomino S. (1994) Structure and developmental expression of a larval cuticle protein gene of the silkworm, Bombyx mori , Biochim. (apidologie.org)
  • A molecular cell biology toolkit for the study of meiosis in the silkworm Bombyx mori. (stowers.org)
  • Structural analysis of neutral glycosphingolipids from the silkworm Bombyx mori and the difference in ceramide composition between larvae and pupae. (ritsumei.ac.jp)
  • 107 and 110 varieties showed the highest expression of HSP70 gene (552 and 53) among female and male silkworm larvae groups, respectively. (ac.ir)
  • disruption of bombyx mori nucleopolyhedrovirus orf71 (bm71) results in inefficient budded virus production and decreased virulence in host larvae. (ijust18.com)
  • In this study, we identified candidate reference genes based on transcriptome data covering embryos and larvae of early development, normal adult tissues, and the hindgut under sulfide stress using the coefficient of variation (CV) method in the echiuran Urechis unicinctus , resulting in 6834 (15.82%), 7110 (16.85%) and 13880 (35.87%) candidate reference genes, respectively. (biomedcentral.com)
  • For expressing foreign genes, a new host- vector system, with Bombyx mori silkworm larvae as the host and B. mori nuclear polyhedrosis virus (BmNPV) as the vector, has been developed. (microbiologyresearch.org)
  • BmNPV genomic DNA codes polyhedrin which is a major protein of inclusion bodies and is mass- produced in infected silkworm larvae. (microbiologyresearch.org)
  • The silkworm larvae infected with the recombinant virus produced 3·6 mg of the fusion protein per larva and the infected BM-N cells produced 0·3 mg per ml of culture. (microbiologyresearch.org)
  • This project is an exciting opportunity to see if the highly digestible protein in insect larvae could help meet that need. (lookformedical.com)
  • Dragon Grub uses highly palatable and digestible Black Soldier Fly larvae, which are high-protein and nutrient-rich insects with an ideal Calcium:Phosphorus ratio. (lookformedical.com)
  • 19. Changes in gene expression and apoptotic response in Spodoptera exigua larvae exposed to sublethal concentrations of Vip3 insecticidal proteins. (lookformedical.com)
  • Identification and Characterization of Genes Involved in Ecdysteroid Esterification Pathway Contributing to the High 20-Hydroxyecdysone Resistance ofHelicoverpa armigera. (cqu.edu.cn)
  • Lampe D.J., Willis J.H. (1994) Characterization of a cDNA and gene encoding a cuticular protein from rigid cuticles of the giant silkmoth, Hyalophora cecropia , Insect Biochem. (apidologie.org)
  • development and characterization of a new bombyx mori cell line for protein expression, journal of asia-pacific entomology. (ijust18.com)
  • Characterization of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from the halotolerant alga Dunaliella salina and inhibition of its expression by RNAi. (shengsci.com)
  • Charles J.-P., Chihara C., Nejad S., Riddiford L.M. (1998) Identification of proteins and developmental expression of RNAs encoded by the 65A cuticle protein gene cluster in Drosophila melanogaster , Insect Biochem. (apidologie.org)
  • G4 and i-motif structures were identified in the promoter of the transcription factor gene BmPOUM2 , which regulates the expression of the wing disc cuticle protein gene (BmWCP4) during metamorphosis in Bombyx mori . (isocce.org)
  • Of the four putative novel defence proteins (DFPs) annotated by PSI-BLAST three showed similarity to extracellular matrix proteins from vertebrates implicated in innate immunity, while the fourth was similar to, yet distinct from, the anti-microbial protein cecropin. (biomedcentral.com)
  • Apple R.T., Fristrom J.W. (1991) 20-hydroxyecdys-one is required for, and negatively regulates, transcription of Drosophila pupal cuticle protein genes, Dev. (apidologie.org)
  • 4. Graham PL, Yanowitz JL, Penn JK, Deshpande G, Schedl P. The translation initiation factor eIF4E regulates the sex-specific expression of the master switch gene Sxl in Drosophila melanogaster. (prolekarniky.cz)
  • 5. Hashiyama K, Hayashi Y, Kobayashi S. Drosophila Sex lethal gene initiates female development in germline progenitors. (prolekarniky.cz)
  • Sexual back talk with evolutionary implications: stimulation of the Drosophila sex-determination gene sex-lethal by its target transformer. (prolekarniky.cz)
  • Autoregulation of the splicing of transcripts from the transformer-2 gene of Drosophila. (prolekarniky.cz)
  • The Drosophila genome contains two genes( CG8422 and CG1237 0) orthologous to Manduca and Acheta DH-Rs. (silverchair.com)
  • By microarray analysis, the CG8422 transcript was detectable in Drosophila head mRNA of different genotypes and under different environmental conditions. (silverchair.com)
  • The identification of a Drosophila receptor for the DH neuropeptide provides a basis for genetic analysis of this critical factor's roles in maintaining physiological homeostasis. (silverchair.com)
  • Here, using the model species Drosophila melanogaster , we used a combination of Genome Wide Association mapping (GWAS) and transcriptomic profiling to characterize whether genes associated with thermal tolerance are primarily involved in dynamic stress responses or preparatory processes that influence physiological condition at the time of thermal stress. (frontiersin.org)
  • A total of 32 differentially expressed proteins were identified by comparing the results of silver-linked mass spectrometry with those of silkworm genome database and NCBI database. (chinaagrisci.com)
  • The result of Southern blot indicated that there isonly one C23O gene in the genome of C-14-1. (researchgate.net)
  • The Bmuccr gene covers a 1.4 kb genome region and contains 3exons. (researchgate.net)
  • Genome-wide analysis of whole human glycoside hydrolases by data-driven analysis in silico │ Int. J. Mol. (ritsumei.ac.jp)
  • Matching to known miRNA alignment, a total of 25 known miRNAs were significantly different between them, with 1 up-regulated and 24 down-regulated in the flying matured virgin queens while there were 11 target genes of 19 known miRNA which were expressed differentially between them. (chinaagrisci.com)
  • 59/99 for heat), and overall GWAS candidates were more likely to be differentially expressed than other genes. (frontiersin.org)
  • S. Gomi, C. E. Zhou, W. Y. Yih, K. Majima, Maeda S. (1997) Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV. (scirp.org)
  • Bombyx mori nuclear polyhedrosis virus (BmNPV) is essentially the most damaging virus for the manufacturing of silkworm cocoons. (nuclearproteomics.org)
  • These results also have utility for directly comparing GWAS and transcriptomic approaches for identifying candidate genes associated with thermal tolerance. (frontiersin.org)
  • Ratios of nonsynonymous to synonymous nucleotide changes (ω) were used to test for selection, as a means of identifying candidate genes within each locus that control wing pattern. (biomedcentral.com)
  • Tests of selection reveal a cluster of candidate genes in each locus, suggesting that weak directional selection may be occurring within a small region of each locus, but coding changes alone are unlikely to explain the full range of wing pattern diversity. (biomedcentral.com)
  • A third of the transcriptome showed similarity with previously characterized immune-related genes that included both the known and putative immune genes. (biomedcentral.com)
  • The present study has identified many potential immune-related genes in A. mylitta some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. (biomedcentral.com)
  • From the transcriptome database of C. rogercresseyi , 57 putative members of ABC protein sequences were identified and phylogenetically classified into the eight subfamilies described for ABC transporters in arthropods. (biomedcentral.com)
  • Here we determine whether 38 putative genes within two major Heliconius patterning loci, HmYb and HmB , show evidence of positive selection. (biomedcentral.com)
  • Prediction of protein antigenic determinants from amino acid sequences. (scirp.org)
  • Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information. (biomedcentral.com)
  • 2014). Where annotations were not present or were different, the online Softberry server (http://linux1.softberry.com/berry.phtml) was used to predict mRNA and protein sequences using the FGNESH+ prediction algorithm (Solovyev et al. (molquest.com)
  • Combining deep sequencing and bioinformatics analysis, the percent of the common sequences in total sRNAs was 92.79%, but the number of unique sRNAs in the flying queens was more than that in non-flying queens. (chinaagrisci.com)
  • The coding sequences of the hepatitis B virus surface antigen were inserted into a baculovirus transfer vector produced from Autographa califomica nuclear polyhedrosis virus (AcNPV) so that the foreign gene was under the control of the AcNPV polyhedrin promoter. (microbiologyresearch.org)
  • Preliminary analyses using 454 transcriptome and Bacterial Artificial Chromosome (BAC) sequences from three Heliconius species highlighted a cluster of genes within each region showing relatively higher rates of sequence evolution. (biomedcentral.com)
  • Most eukaryotic protein coding genes are intervened by non-coding sequences called introns ( Int ervening r egi ons ) [ 1 ], which are being removed from the primary transcript in the process of splicing [ 2 - 3 ]. (ijbs.com)
  • Finally, we analysed the expression profiles of 15 potential immune-related genes, and the majority of them were induced more prominently with E. coli compared to Micrococcus luteus . (biomedcentral.com)
  • Notably, 3D20E transfer also induces expression of a reproductive gene that preserves egg development during Plasmodium infection, ensuring fitness of infected females. (nature.com)
  • Additionally, histopathological analysis and immunohistochemical alterations in the expression of caspase-3 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-4 (NOX-4) in kidney tissue were performed to confirm the findings of the in vivo experiments. (bvsalud.org)
  • In the current study, we cloned and expressed the gene encoding silkworm SP2 ( sp2 ) both in a prokaryotic expression system and a silkworm baculovirus system. (hindawi.com)
  • Expression of the polh gene of AnpeNPV in E. coli was examined by SDS-PAGE, Western blot and Mass-spectrum analysis. (scirp.org)
  • The result showed that the bacterium expression system was suitable for the virus gene expression. (scirp.org)
  • The BmHSP60 candidate interaction protein clone expression vector was constructed and fused with HA tag, and co-transfected with BmHSP60 into B. mori cells. (chinaagrisci.com)
  • Hiruma K., Hardie J., Riddiford L.M. (1991) Hormonal regulation of epidermal metamorphosis in vitro: control of expression of a larval-specific cuticle gene, Dev. (apidologie.org)
  • Nakato H., Izumi S., Tomino S. (1992) Structure and expression of gene coding for a pupal cuticle protein of Bombyx mori , Biochim. (apidologie.org)
  • Rebers J.E., Niu J., Riddiford L.M. (1997) Structure and spatial expression of the Manduca sexta MSCP14.6 cuticle gene, Insect Biochem. (apidologie.org)
  • gene cloning and expression analysis of the magpx family in morus alba l .acta botanica boreali-occidentalia sinica, 2019,3(6): 0991-1000. (ijust18.com)
  • expression and function analysis of mulberry osmotin gene. (ijust18.com)
  • Loss of Bmdsx gene expression significantly reduced the peripheral perception of the major pheromone component bombykol by reducing expression of the product of the BmOR1 gene which completely blocked courtship in adult males. (prolekarniky.cz)
  • For example, we found that expression of TK1 , a potential target gene of a MYBL2-linked enhancer, is associated with poor patient survival. (prolekarniky.cz)
  • Animal steroid hormones initiate signaling by passive diffusion into cells and binding to their nuclear receptors to regulate gene expression. (biologists.com)
  • In addition, RT-qPCR experiments with the other main clock genes showed that pdp1 , antagonist of vrille in the modulatory feedback loop of the circadian clock, showed higher expression levels in the rice-strain than in the corn-strain. (biomedcentral.com)
  • Real-time quantitative PCR (RT-qPCR) is a crucial and widely used method for gene expression analysis. (biomedcentral.com)
  • The expression stability of the ten genes was analyzed using four methods (geNorm, NormFinder, BestKeeper, and ∆Ct). (biomedcentral.com)
  • Our study is the first to select reference genes for RT-qPCR from transcriptome data in Echiura and provides important information for future gene expression studies in U. unicinctus . (biomedcentral.com)
  • Reference genes are used for the normalization of gene expression because of the stability of expression levels among different tissues, different developmental stages, or under various treatments [ 5 ]. (biomedcentral.com)
  • Aim】 To prepare polyclonal antibody of the fungal-resistance factor BmSPI39 in Bombyx mori and to analyze its expression in response to Beauveria bassiana invasion. (insect.org.cn)
  • Methods】 Prokaryotic expression and immobilized nickel ion affinity chromatography were used to obtain the highly-purified recombinant BmSPI39 protein. (insect.org.cn)
  • The heat map analysis of the expression data of BmSPI39 showed that BmSPI39 was expressed in the integument of B. mori at the wandering stage and the pre-pupal stage, with a higher expression level in the fat body at the pre-pupal stage, but not in the midgut at all the tested developmental stages. (insect.org.cn)
  • Expression of the fungal-resistance factor BmSPI39 in Bombyx mori in response to Beauveria bassiana invasion [J].Acta Entomologica Sinica, 2021, 64(1): 61-69. (insect.org.cn)
  • An ectotherm's thermal tolerance is determined by both fixed genetic factors and plastic changes in behavior, morphology, physiology, and gene expression. (frontiersin.org)
  • The theoretical basis for this hypothesis is that every gene possesses a modular arrangement of 5' CREs that control temporal and spatial patterns of expression, and that these regions are less subject to the negative pleiotropic effects constraining coding changes, allowing adaptive evolution to proceed at a higher rate [ 10 ]. (biomedcentral.com)
  • R. D. Possee, S. C. Howard, (1987) Analysis of the polyhedrin gene promoter of the Autographa californica nuclear polyhedro-sis virus. (scirp.org)
  • Binger L.C., Willis J.H. (1994) Identification of the cDNA, gene and promoter for a major protein from flexible cuticles of the giant silkmoth Hyalophora cecropia , Insect Biochem. (apidologie.org)
  • Both proteins were expressed using a construct in which a promoter was inserted upstream of each gene. (shengsci.com)
  • This fusion protein gene was combined in a plasmid with the promoter and 5′ and 3′ flanking regions of the polyhedrin gene. (microbiologyresearch.org)
  • Interestingly, we found that mating behavior was regulated independently by another sexual differentiation gene, Bmfru . (prolekarniky.cz)
  • Identification of genes involved in sex pheromone biosynthesis and metabolic pathway in the Chinese oak silkworm, Antheraea pernyi. (cqu.edu.cn)
  • 7. Valcárcel J, Singh R, Zamore PD, Green MR. The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA. (prolekarniky.cz)
  • The genes were introduced as an operon, but although mRNA was detected for both genes, no Cyt1Aa toxin was detected. (shengsci.com)
  • E. A. van Strien, D. Zuidema, R.W. Goldbach, Vlak, J. M. (1992) Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis vi-rus. (scirp.org)
  • Label-free proteomic analysis of silkworm midgut infected by Bombyx mori nuclear polyhedrosis virus. (nuclearproteomics.org)
  • Several genes were present as members of gene families, as has also been observed in other insect species. (biomedcentral.com)
  • Totally, 38 representative species in evolution were selected for genomic analysis of the DNA secondary structure, distribution and evolution. (isocce.org)
  • Three genes from each locus with the highest average pairwise ω values were amplified from additional Heliconius species and races. (biomedcentral.com)
  • Two selected genes, fizzy-like ( HmYb ) and DALR ( HmB ), were too divergent for amplification across species and were excluded from further analysis. (biomedcentral.com)
  • Algal proteins are sourced from microalgae or single-cell microorganisms containing up to 70% proteins from species such as chlorella or spirulina. (lookformedical.com)
  • METHODS: Ticks submitted by study participants from April-September 2011 and 2012 were identified to species and life stage, and preliminarily screened for the genus Rickettsia by nested PCR targeting the 17-kDa protein gene. (lookformedical.com)
  • We utilized the YI , Y+ I , + Y I and + Y + I mutants of Bombyx mori to characterize lipid absorption and transport from midgut to silk glands via lipophorin. (confex.com)
  • The target genes of the regulatory cascade include genes of various functions required for morphological development and secondary metabolite formation. (go.jp)
  • The co-localization of BmHSP60 candidate interaction proteins with mitochondria was analyzed by Mito-Tracker-Green. (chinaagrisci.com)
  • Conclusion】 Heat shock protein BmHSP60 of B. mori was identified to interact with BmANT and BmHSP90, and co-localized in mitochondria. (chinaagrisci.com)
  • further, functional analysis revealed that loss of the product of BmOR3 played a key role in terminating male mating behavior. (prolekarniky.cz)
  • Here, we present multiple lines of evidence to support the hypothesis that the orphan CG8422 G-protein-coupled receptor is a functional DH-R. When expressed in mammalian cells, CG8422 conferred selective sensitivity to DH, as indicated by translocation of aβ-arrestin-2-GFP reporter from the cytoplasm to the cell membrane. (silverchair.com)
  • Insect genes that harbor U12-type introns belong to several functional categories among which proteins binding ions and nucleic acids are enriched and these few categories are also overrepresented among these genes that preserved minor type introns in Diptera . (ijbs.com)
  • Their arrangement was consistent with the first sequenced mitogenome of Lepidoptera, from Bombyx mori (Bombycidae). (authorea.com)
  • Palli S.R., Locke M. (1987) The synthesis of hemolymph-proteins by the larval epidermis of an insect Calpodes ethlius (Lepidoptera, Hesperiidae), Insect Biochem. (apidologie.org)
  • This result shows that the mini gene contained the information necessary for the correct regulation of alternative splicing. (bioone.org)
  • The results of this study provide new insights into the molecular mechanism of the secondary structures in epigenetic regulation of gene transcription. (isocce.org)
  • Further, several GWAS candidates were regulatory genes that may participate in the regulation of stress responses, and gene ontologies related to development and morphogenesis were enriched, suggesting many of these genes influence thermal tolerance through effects on development and physiological status. (frontiersin.org)
  • In the present study we constructed and analysed the immune transcriptome of the lepidopteran Antheraea mylitta , an economically important Indian tasar silkmoth with a view to unravel the potential immune-related genes and pathways. (biomedcentral.com)
  • Definition of the homologous of bovine subunit VII of ubiquinol-cytochrome C reductaseshould facilitate further analysis of structure/function relationships of silkworm cytochrome bcl complex. (researchgate.net)
  • Using RADtags, we identified this QTC to be homologous to Bombyx mori C27, on which the clock gene vrille is located, which thus became the major candidate gene. (biomedcentral.com)
  • The present study gives a comprehensive transcriptome analysis of ABC proteins from C. rogercresseyi, providing relevant information about transporter roles during ontogeny and in relation to delousing drug responses in salmon lice. (biomedcentral.com)
  • With the increasing application of high-throughput sequencing, transcriptome analysis has become an effective method for identifying novel stable reference genes. (biomedcentral.com)
  • Kiely M.L., Riddiford L.M. (1985) Temporal programming of epidermal cell protein synthesis during the larval-pupal transformation of Manduca sexta , Wilhelm Roux's, Arch. (apidologie.org)
  • Ochieng V.O., Osir E.O., Ochanda J.O., Olembo N.K. (1993) Temporal synthesis of cuticle proteins during larval development in Glossina morsitans , Comp. (apidologie.org)
  • Cox D.C., Willis J.H. (1985) The cuticular proteins of Hyalophora cecropia from different anatomical regions and metamorphic stages, Insect Biochem. (apidologie.org)
  • Western blotting analysis demonstrated the presence of CBP only in the yellow colored tissues of the silk gland, midgut, testis and ovary. (confex.com)
  • Gloverin is an inducible antibacterial insect protein which inhibits the synthesis of vital outer membrane proteins leading to a permeable outer membrane. (wikipedia.org)
  • The most persuasive roles for HSV and HCMV in human tumourigenesis are as mutagens, as activators of cellular transcription or in switching on the synthesis of host cell proteins not normally expressed in untransformed cells. (microbiologyresearch.org)
  • Zinc finger protein 483 (ZNF483) regulates neuronal differentiation and methyl-CpG-binding protein 2 (MeCP2) intracellular localization. (ritsumei.ac.jp)
  • W. B. Wang, S. Y. Zhu, L. Q. Wang, F. Yu, Shen W. D. (2005) Cloning and sequence analysis of the Antheraea pernyi nucleo-polyhedrovirus gp64 gene. (scirp.org)
  • The number of the G4 motif in the upstream regulatory region of the genes increased from lower to higher organisms. (isocce.org)
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the candidate reference genes were significantly enriched in cellular metabolic process, protein metabolic process and ribosome in early development and normal adult tissues as well as in cellular localization and endocytosis in the hindgut under sulfide stress. (biomedcentral.com)
  • The spliceosomes consist of four small nuclear ribonucleoproteins (snRNPs) and over a hundred of non-snRNP proteins that associate with snRNPs at some point during the splicing [ 4 - 6 ]. (ijbs.com)
  • according to morphological, physiological and its 16S rRNA gene sequence. (researchgate.net)
  • Morphological transformation studies have failed to demonstrate a viral oncogene, a virus-coded transforming protein or any sequence of DNA that uniquely transforms cells according to one-hit kinetics. (microbiologyresearch.org)
  • IGF II was released from the fusion protein produced by BM-N cells infected with the recombinant virus by CNBr treatment, purified by extraction with guanidine-HCl, column chromatography and HPLC and the correct amino-terminal amino acid sequence confirmed. (microbiologyresearch.org)
  • As one such antiapoptotic component in silkworm hemolymph, the 30 K protein has been studied widely [ 6 , 7 ]. (hindawi.com)
  • A recent study showed that another protein in silkworm hemolymph, storage protein 2 (SP2), can also inhibit staurosporine-induced HeLa cell apoptosis and ROS generation [ 8 ]. (hindawi.com)
  • Here by using CRISPR/Cas9 and electrophysiological techniques we found that courtship and mating behaviors are regulated in male silk moths by mutating genes in the sex determination cascade belonging to two conserved pathways. (prolekarniky.cz)
  • 3. Gempe T, Beye M. Function and evolution of sex determination mechanisms, genes and pathways in insects. (prolekarniky.cz)
  • Knockdown experiments of FOXM1 and MYBL2 suggest that these factors regulate genes involved in controlling cell cycle progression and cell division. (prolekarniky.cz)
  • The nucleotide composition was obviously AT-biased, and all protein-coding genes, except for the cox1 gene (CGA), used ATN as the start codon. (authorea.com)
  • Objective】 HSP60 is an important member of heat shock proteins, which plays an important role in the innate immunity of insects, and is also an essential factor for insect growth and development. (chinaagrisci.com)
  • The objective of this study is to identify the interaction proteins of BmHSP60, and to provide a basis for further exploring the mechanism in the innate immunity of B. mori . (chinaagrisci.com)
  • As a first step toward discovery of the cis-element, the Bmdsx mini gene, which consisted of exon 1 and 5 and internally shortened introns 2 to 4, was constructed, and transgenic silkworms expressing this construct were generated. (bioone.org)
  • Transcriptomic profiles for ABC proteins subfamilies were evaluated throughout C. rogercresseyi development. (biomedcentral.com)
  • Through QTL analyses we identified one major Quantitative trait chromosome (QTC) underlying differentiation in circadian timing of mating activity. (biomedcentral.com)
  • In this study, the sp2 gene was cloned and expressed in Escherichia coli to produce a 6xHis-tagged fusion protein, which was then used to generate a polyclonal antibody. (hindawi.com)
  • The expressed protein in Escherichia coli was used to generate polyclonal antibodies. (hindawi.com)
  • Enzymes of various catalytic functions in Streptomyces are useful as members of an artificial gene cluster constructed in Escherichia coli for fermentative production of plant-specific flavonoids, including isoflavones and unnatural compounds. (go.jp)
  • ATP-binding cassette (ABC) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane. (biomedcentral.com)
  • Laemmli U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685. (apidologie.org)
  • In this gene an ATG codon for methionine was introduced for cleavage by CNBr at the beginning of mature IGF II. (microbiologyresearch.org)
  • The biosynthesis gene clusters for grixazone and hexahydroxyperylenequinone are examples. (go.jp)
  • We also identified several unknown proteins, some of which could have probable immune-related functions based on the results of the ProDom analysis. (biomedcentral.com)
  • The size of the bands was aligned with the NCBI database to screen for candidate interaction proteins according to the mass spectrometry results. (chinaagrisci.com)
  • In this work, we use both approaches to study drug-albumin binding and support the results by comparison with other complementary techniques and by structural and computational analysis. (mdpi.com)
  • Together, our results indicate that the allochronic differentiation in the two strains of S. frugiperda is associated with differential transcription of vrille or a cis-acting gene close to vrille, which contributes to the evolution of prezygotic isolation in S. frugiperda . (biomedcentral.com)
  • Selecting suitable reference genes is extremely important for the accuracy of RT-qPCR results. (biomedcentral.com)
  • The comprehensive results indicated that the new candidate reference genes were more stable than most commonly used reference genes. (biomedcentral.com)
  • Therefore, two or more reference genes are desirable to improve the reliability and accuracy of the RT-qPCR results. (biomedcentral.com)
  • However, these results have been underestimated because genes for the two key enzymes of the cycle, isocitrate lyase (ICL) and malate synthase (MS), are not annotated in Leishmania genomes. (degruyter.com)
  • Results】 The results of in-gel activity staining showed that the recombinant BmSPI39 protein could strongly inhibit the activity of subtilisin A. Enzyme-linked immunosorbent assay (ELISA) showed that the antibody titer of BmSPI39 antiserum reached 1∶128 000. (insect.org.cn)
  • The results of Western blot and in-gel activity staining showed that the purified BmSPI39 antibody could effectively bind to the BmSPI39 antigen protein in different multimerization states. (insect.org.cn)
  • The results support previous research suggesting that insect protein has the greatest potential to reduce the food-related carbon footprints of European consumers, if edible insects - such as crickets, flies, and worms - are consumed directly or processed as food. (lookformedical.com)
  • Our results indeed suggest that it is more sustainable to use insect protein for food rather than to use it to replace soybean meal in animal feed. (lookformedical.com)
  • This work will lay a foundation for the development and utilization of protein drugs from economically important insects for treatment of vascular diseases. (hindawi.com)
  • Compared to other insects, Bombyx mori , the domesticated silkworm, has relatively simple sexual behaviors as it cannot fly. (prolekarniky.cz)
  • Insects are a noteworthy sustainable source of animal protein for food and animal feed. (lookformedical.com)
  • Genetic and genomic analysis for cocoon yield traits in silkworm. (cqu.edu.cn)
  • Protein-coding change is one possible genetic mechanism underlying the evolution of adaptive wing colour pattern variation in Heliconius butterflies. (biomedcentral.com)
  • With reference to insect immunity, the ESTs have been obtained from baculovirus-infected B. mori cultured cells and pupae, but no large scale information on bacteria-induced immune genes is as yet available. (biomedcentral.com)
  • Cells infected with this recombinant virus (gG-VAC) synthesized glycosylated proteins of 48K, 57K and 61K mol. (microbiologyresearch.org)
  • proteins in HSV-1-infected cells. (microbiologyresearch.org)
  • Made from insect protein, one of the most digestible and sustainable forms of protein on the planet. (lookformedical.com)
  • Genomic analysis and physicochemical screening of ikarugamycin, fumaquinone, and a new compound, pudicin from Streptomyces sp. (scienceasia.org)
  • A strongly hydrophilic region was predicted at positions from 30 to 50 of the An-peNPV Polh protein by bioinformatics analysis. (scirp.org)
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