• Inquiries about various issues relating to Western blot (WB) testing are frequently posted to the Lyme disease discussion groups on the Internet. (google.com)
  • Western blotting in the serodiagnosis of Lyme disease. (nih.gov)
  • There are currently no accepted criteria for positive Western blots in Lyme disease. (nih.gov)
  • Indeed, Western blotting has been used at one time or the other to aid in the diagnosis of infectious diseases including hepatitis C (HCV), HIV, Lyme disease, and syphilis, as well as autoimmune disorders such as paraneoplastic disease and myositis conditions. (aacc.org)
  • The Odyssey Fc System is the only system that offers superior chemiluminescent detection and the benefits of infrared western blot detection as well as the ability to document DNA gels using the 600nm channel. (selectscience.net)
  • 5. Place the pre-run gels onto the transfer membrane making sure that the gel marker is facing toward you and use the Blotting Roller to remove all air bubbles. (peprotech.com)
  • 6. Place the pre-soaked (in deionized water) iBlot Filter Paper onto the pre-run gels and remove air bubbles using the Blotting Roller. (peprotech.com)
  • In fact, in the third sentence of their abstract ( 1 ) they wrote, "For sodium dodecyl sulfate gels, the original band pattern was obtained with no loss of resolution, but the transfer was not quantitative," suggesting that they considered accurate protein quantification by means of Western blotting to be a challenge. (mcponline.org)