• The northern blot, or RNA blot, is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. (wikipedia.org)
  • Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)
  • RNA was extracted from some cells and the extent of expression of inducible NOS mRNA was determined by Northern blotting. (cdc.gov)
  • The predicted size of the COMT transcript was 1.8-2.0 kb, which could be confirmed from Northern hybridization analyses of the isolated rat liver mRNA. (lu.se)
  • Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. (wikipedia.org)
  • Strictly speaking, the term 'northern blot' refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. (wikipedia.org)
  • Southern and Northern blots and reverse-transcription PCR analyses indicate that the same rhodopsin gene is expressed in the retina and the brain but not in the pineal organ of ayu. (bioone.org)
  • These cells were characterised by PCR, immunocytochemistry, electron microscopy, and Western and Northern blot analyses. (ersjournals.com)
  • We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)
  • Northern hybridization is used to measure the quantity and dimension of RNAs transcribed from eukaryotic genes and to estimate their abundance. (humccancer.org)
  • It is also known as Northern hybridization, or RNA hybridization. (asu.edu)
  • Component of hybridization solutions for Northern and Southern blotting. (nationaldiagnostics.com)
  • It is especially designed for use in Southern and Northern Blotting, and in situ hybridization procedures. (nationaldiagnostics.com)
  • Commonly, nucleic acids or proteins are electrophoretically separated on agarose or acrylamide gels and then transferred (blotted) from the gel onto the NC. (cdc.gov)
  • Rapid transfer times Western, Southern and Northern Blots Economic Transfers due to very low buffer volumes Screw down lid Gels from 0.25 up to 10mm thick can be blotted Uniform heat dispersion Long life electrodes OVERVIEW These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting typically 15 to 30 minutes. (medicalexpo.com)
  • Such terms as 'Southern' (transfer of DNA), 'Northern' (transfer of RNA), and 'Western' (transfer of protein) blots have been coined. (cdc.gov)
  • All units can be used for all types of blotting: Western, Southern and Northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. (medicalexpo.com)
  • We used quantitative Western blotting to observe Lcn2 serum levels in lean and obese mouse models. (diabetesjournals.org)
  • ABSTRACT Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross- reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
  • ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
  • Northern analysis detected a specific 2.5-kilobase pair hOAT1 transcript only in human kidney. (aspetjournals.org)
  • Southern blot analysis of total cell DNA of the transformants shows that the wild type psaC gene has been replaced by the interrupted psaC gene through homologous recombination. (unige.ch)
  • Employing the DNA blot analysis only one COMT-encoding gene was found in the rat genome. (lu.se)
  • A general blotting procedure starts with extraction of total RNA from a homogenized tissue sample or from cells. (wikipedia.org)
  • The procedure for and theory behind Northern blotting is almost identical to that of Southern blotting, except you are working with RNA instead of DNA. (asu.edu)
  • Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. (wikipedia.org)
  • The transfer buffer used for the blotting usually contains formamide because it lowers the annealing temperature of the probe-RNA interaction, thus eliminating the need for high temperatures, which could cause RNA degradation. (wikipedia.org)
  • Washing buffer for Northern and Southern blotting. (nationaldiagnostics.com)
  • Since the gels are fragile and the probes are unable to enter the matrix, the RNA samples, now separated by size, are transferred to a nylon membrane through a capillary or vacuum blotting system. (wikipedia.org)
  • A nylon membrane with a positive charge is the most effective for use in northern blotting since the negatively charged nucleic acids have a high affinity for them. (wikipedia.org)
  • With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression rates during differentiation and morphogenesis, as well as in abnormal or diseased conditions. (wikipedia.org)
  • Transfer-messenger RNA (tmRNA) expression was recorded for each blot for normalization against internal loading controls. (cdc.gov)
  • Activation of Xwnt11 is induced in an immediate-early fashion by Xbra and its expression in vivo is abolished by a dominant-interfering form of Xbra , Xbra -En(R). Overexpression of a dominant-negative form of Xwnt11 , like overexpression of Xbra -En(R), inhibits convergent extension movements. (xenbase.org)
  • To create controls for comparison in a northern blot, samples not displaying the gene product of interest can be used after determination by microarrays or RT-PCR. (wikipedia.org)
  • The gels can be stained with ethidium bromide (EtBr) and viewed under UV light to observe the quality and quantity of RNA before blotting. (wikipedia.org)
  • Commonly cDNA is created with labelled primers for the RNA sequence of interest to act as the probe in the northern blot. (wikipedia.org)
  • QIAamp technology yields total cellular RNA from fresh whole blood and other sample sources that is ready to use in RT-PCR and blotting procedures. (qiagen.com)
  • NC sheets up to 20 x 20 cm are often used in these blotting procedures. (cdc.gov)
  • Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. (medicalexpo.com)
  • These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. (medicalexpo.com)
  • Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. (medicalexpo.com)
  • The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. (wikipedia.org)
  • This technique came to be known as Southern blotting. (asu.edu)
  • Is the Subject Area "Northern blot" applicable to this article? (plos.org)
  • Instead, it will blot out its center, leaving a ring at the edges. (popsci.com)
  • Notable among these were a study of hard-metal workers in several small factories in northern Italy. (who.int)
  • The lower expression levels of the SCD1 gene in both diabetic groups compared with controls were further confirmed by Northern blot analysis and immunohistochemistry. (diabetesjournals.org)
  • Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. (wikipedia.org)
  • [ 37 ] Northern membrane was probed by using corresponding hybridization fragments of mouse Cx36, mouse Cx45 [ 38 ] and human Cx59 (1.5 kb) as well as human Cx62 (1.6 kb) genomic DNA (this study). (medscape.com)
  • Subsequently, the amounts of total RNA on the Northern blot were standardized by hybridization of to a probe of glyceraldehydephosphate dehydrogenase (GAPDH). (medscape.com)
  • This was analysed by Northern hybridization with a 280 base pair probe containing sequences derived from exons 3 and 4 of the insulin-like growth factor 1 gene. (nih.gov)
  • A cDNA encoding a defensin-like peptide, Coprisin, was isolated from bacteria-immunized dung beetle, Copris tripartitus , by using differential dot blot hybridization. (hindawi.com)
  • In this study, we constructed a full-length cDNA library from bacteria-immunized C. tripartitus and then selected up-regulated clones using differential screening by a dot blot hybridization analysis. (hindawi.com)
  • Coprisin cDNA clones were identified by a dot blot hybridization. (hindawi.com)
  • Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. (bvsalud.org)
  • Protein expression will be analyzed by immunohistochemistry (for localization) and western blotting (for quantification). (nih.gov)
  • A general blotting procedure starts with extraction of total RNA from a homogenized tissue sample or from cells. (wikipedia.org)
  • The amounts of total RNA on the Northern blot were roughly standardized by determination of the intensities of the ethidium bromide stained 18S- and 28S rRNA. (medscape.com)
  • Since the gels are fragile and the probes are unable to enter the matrix, the RNA samples, now separated by size, are transferred to a nylon membrane through a capillary or vacuum blotting system. (wikipedia.org)
  • A nylon membrane with a positive charge is the most effective for use in northern blotting since the negatively charged nucleic acids have a high affinity for them. (wikipedia.org)
  • Probes for northern blotting are composed of nucleic acids with a complementary sequence to all or part of the RNA of interest. (wikipedia.org)
  • The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. (sigmaaldrich.com)
  • Commonly, nucleic acids or proteins are electrophoretically separated on agarose or acrylamide gels and then transferred (blotted) from the gel onto the NC. (cdc.gov)
  • She has hardware and software which scans the autoradiogram coming out of Northern blot analysis into a TIFF file on her PC. (bio.net)
  • TNF-α gene transcription was monitored by Northern blot analysis. (portlandpress.com)
  • In some experiments, changes in NEP-specific mRNAs in the presence or absence of dexamethasone and/or the inhibitors were also evaluated by Northern blot analysis. (cdc.gov)
  • Northern blot analysis indicated that NEP-specific mRNAs were also increased by dexamethasone. (cdc.gov)
  • Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain. (thermofisher.com)
  • Northern-blot analysis of poly(A)+ RNA from several human tissues indicates a complex expression pattern differing across tissues. (tcdb.org)
  • About 10 years ago, the Maraia Lab developed an assay, based on a northern blot, to identify these types of mutations in tRNA modification enzymes (TME). (nih.gov)
  • Falsified the results for NT2 binding to the Col11a2 DNA sequence in electrophoretic mobility shift assays in Figures 2D and 6B, and falsified the Western blot for NT2 mutant proteins in Figure 8B in MCB 22:4256-4267, 2002. (nih.gov)
  • Western blotting was used to confirm asymptomatic VL ( 8 ). (cdc.gov)
  • Southern blotting was introduced by Edwin Southern in 1975 as a method to detect specific sequences of DNA in DNA samples. (sigmaaldrich.com)
  • Commonly cDNA is created with labelled primers for the RNA sequence of interest to act as the probe in the northern blot. (wikipedia.org)
  • 20X SSC (93017) may be used in place of SSPE in similar blotting protocols. (sigmaaldrich.com)
  • The transfer buffer used for the blotting usually contains formamide because it lowers the annealing temperature of the probe-RNA interaction, thus eliminating the need for high temperatures, which could cause RNA degradation. (wikipedia.org)
  • Our laboratory developed a northern blot method that can quantify relative levels of base modifications on multiple specific tRNAs ∼10 yr ago, which has been used to characterize four different TME deficiencies and is likely further extendable. (nih.gov)
  • HVL has not been reported) and 11 sites (2,086 serum samples) in the HVL-endemic northern region, where the disease was previously reported in 8 of 11 sites ( Figure ). (cdc.gov)
  • Falsified the Northern blots in multiple panels of Figure 3, MCB 20:4428-4435, 2000. (nih.gov)
  • In this study, HVL seroprevalence in northern Israel was compared with that in a region free of clinical disease. (cdc.gov)
  • Your comments on the use of these packages, in light of our Northern blot application, will be of interest to us. (bio.net)
  • Harris, CJ, Baulcombe, DC & Molnar, A 2017, Improved denaturation of small RNA duplexes and it's application for northern blotting . (ed.ac.uk)
  • However, the entire process is commonly referred to as northern blotting. (wikipedia.org)
  • In 1815, Mount Tambora erupted and its emissions spread across the globe, blotting out the sun for almost an entire year. (eschoolnews.com)
  • The major difference is that RNA, rather than DNA, is analyzed in the northern blot. (wikipedia.org)