• The northern blot, or RNA blot, is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. (wikipedia.org)
  • Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. (wikipedia.org)
  • Northern hybridization is used to measure the quantity and dimension of RNAs transcribed from eukaryotic genes and to estimate their abundance. (humccancer.org)
  • Component of hybridization solutions for Northern and Southern blotting. (nationaldiagnostics.com)
  • It is especially designed for use in Southern and Northern Blotting, and in situ hybridization procedures. (nationaldiagnostics.com)
  • The transfer buffer used for the blotting usually contains formamide because it lowers the annealing temperature of the probe-RNA interaction, thus eliminating the need for high temperatures, which could cause RNA degradation. (wikipedia.org)
  • Rapid transfer times Western, Southern and Northern Blots Economic Transfers due to very low buffer volumes Screw down lid Gels from 0.25 up to 10mm thick can be blotted Uniform heat dispersion Long life electrodes OVERVIEW These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting typically 15 to 30 minutes. (medicalexpo.com)
  • All units can be used for all types of blotting: Western, Southern and Northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. (medicalexpo.com)
  • Washing buffer for Northern and Southern blotting. (nationaldiagnostics.com)
  • Such terms as 'Southern' (transfer of DNA), 'Northern' (transfer of RNA), and 'Western' (transfer of protein) blots have been coined. (cdc.gov)
  • These cells were characterised by PCR, immunocytochemistry, electron microscopy, and Western and Northern blot analyses. (ersjournals.com)
  • We used quantitative Western blotting to observe Lcn2 serum levels in lean and obese mouse models. (diabetesjournals.org)
  • ABSTRACT Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross- reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
  • ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
  • With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression rates during differentiation and morphogenesis, as well as in abnormal or diseased conditions. (wikipedia.org)
  • The gels can be stained with ethidium bromide (EtBr) and viewed under UV light to observe the quality and quantity of RNA before blotting. (wikipedia.org)
  • Commonly cDNA is created with labelled primers for the RNA sequence of interest to act as the probe in the northern blot. (wikipedia.org)
  • Northern analysis detected a specific 2.5-kilobase pair hOAT1 transcript only in human kidney. (aspetjournals.org)
  • As new miRNAs are discovered, custom microarray chips accommodate the entire set of known miRNAs on a single chip allowing for rapid and reliable high-throughput analysis that have been validated on more widely accepted platforms such as qPCR and Northern blots. (bitesizebio.com)
  • NC sheets up to 20 x 20 cm are often used in these blotting procedures. (cdc.gov)
  • Strictly speaking, the term 'northern blot' refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. (wikipedia.org)
  • These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. (medicalexpo.com)
  • Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. (medicalexpo.com)
  • Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. (medicalexpo.com)
  • Transfer-messenger RNA (tmRNA) expression was recorded for each blot for normalization against internal loading controls. (cdc.gov)
  • Since the gels are fragile and the probes are unable to enter the matrix, the RNA samples, now separated by size, are transferred to a nylon membrane through a capillary or vacuum blotting system. (wikipedia.org)
  • A general blotting procedure starts with extraction of total RNA from a homogenized tissue sample or from cells. (wikipedia.org)
  • To create controls for comparison in a northern blot, samples not displaying the gene product of interest can be used after determination by microarrays or RT-PCR. (wikipedia.org)
  • Strictly speaking, the term 'northern blot' refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. (wikipedia.org)
  • Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. (wikipedia.org)
  • Fragments of DNA and RNA molecules separated by gel electrophoresis are transferred to a nylon or nitrocellulose membrane in a process termed as Southern and Northern blotting, respectively. (sigmaaldrich.com)
  • After the DNA samples are separated by gel electrophoresis, they are passed on to a membrane by blotting. (1800wheelchair.com)
  • A nylon membrane with a positive charge is the most effective for use in northern blotting since the negatively charged nucleic acids have a high affinity for them. (wikipedia.org)
  • Probes for northern blotting are composed of nucleic acids with a complementary sequence to all or part of the RNA of interest. (wikipedia.org)
  • The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. (sigmaaldrich.com)
  • Commonly, nucleic acids or proteins are electrophoretically separated on agarose or acrylamide gels and then transferred (blotted) from the gel onto the NC. (cdc.gov)
  • Since the gels are fragile and the probes are unable to enter the matrix, the RNA samples, now separated by size, are transferred to a nylon membrane through a capillary or vacuum blotting system. (wikipedia.org)
  • Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. (bvsalud.org)
  • The lower expression levels of the SCD1 gene in both diabetic groups compared with controls were further confirmed by Northern blot analysis and immunohistochemistry. (diabetesjournals.org)
  • Protein expression will be analyzed by immunohistochemistry (for localization) and western blotting (for quantification). (nih.gov)
  • A general blotting procedure starts with extraction of total RNA from a homogenized tissue sample or from cells. (wikipedia.org)
  • The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. (wikipedia.org)
  • Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. (wikipedia.org)
  • Southern Blotting - Southern Blotting is the technique for finding out a particular DNA sequence which is present in a DNA sample. (1800wheelchair.com)
  • A brief overview of a Southern Blot technique. (1800wheelchair.com)
  • Northern Blotting - The northern blotting technique helps in the better understanding of a specific RNA molecule's expression patterns. (1800wheelchair.com)
  • Southern blotting was introduced by Edwin Southern in 1975 as a method to detect specific sequences of DNA in DNA samples. (sigmaaldrich.com)
  • Our laboratory developed a northern blot method that can quantify relative levels of base modifications on multiple specific tRNAs ∼10 yr ago, which has been used to characterize four different TME deficiencies and is likely further extendable. (nih.gov)
  • To create controls for comparison in a northern blot, samples not displaying the gene product of interest can be used after determination by microarrays or RT-PCR. (wikipedia.org)
  • However, the entire process is commonly referred to as northern blotting. (wikipedia.org)
  • The major difference is that RNA, rather than DNA, is analyzed in the northern blot. (wikipedia.org)
  • With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression rates during differentiation and morphogenesis, as well as in abnormal or diseased conditions. (wikipedia.org)
  • An online browser, called 'Theta-Base' ( www.helmholtz-hiri.de/en/datasets/bacteroides ), is launched to interrogate the obtained gene expression data and annotations of ~4500 transcription start sites, untranslated regions, operon structures, and 269 noncoding RNA elements. (nature.com)
  • RNA expression will be analyzed by RT-PCR (for screening) and northern blot (for quantification). (nih.gov)