Loading...
  • VEGF
  • Blockade of VEGF-A signalling hastened barrier formation at the growing vascular front, suggesting that the BRB can only fully formed following completion of the superficial vascular plexus and the concomitant down-regulation of VEGF-A levels. (arvojournals.org)
  • Mechanisms
  • At later stages, two more mechanisms were at work: (1) fenestrae developed in the intraepithelial capillaries, and (2) the RPE attenuated, losing its barrier function. (arvojournals.org)
  • The potential mechanisms linking retinal neurodegeneration and early microvascular impairment, and the effects of neuroprotective drugs are summarised. (springer.com)
  • One of the biggest barriers to treating ALS is that its progression is dynamic-many different cell types and mechanisms are involved-so treating it at one stage of the disease might have very different, and potentially harmful, consequences at a different stage," said Tom Maniatis, PhD, a principal investigator at Columbia's Mortimer B. Zuckerman Mind Brain Behavior Institute and the study's senior author. (medicalxpress.com)
  • ocular
  • Retinas of Long Evans rats were documented with bright-field imaging, FA, & OCT. Some animals received ERG, FA and OCT during a single session in an advanced ocular imaging suite of the Pediatric Retinal Research Laboratory. (arvojournals.org)
  • However, because of early retinal vascular defects in the knockout models, it has not been possible to study FZD4 contribution in ocular neovascular disease. (arvojournals.org)
  • Methods
  • Unlike conventional methods for measuring the TEER where electrodes are on each side of the cell barrier, a method with only electrodes on the substrate has been validated. (rsc.org)
  • breakdown
  • This condition is referred to as blood retinal barrier (BRB) breakdown. (hindawi.com)
  • We are investigating immunopathomechanisms of equine recurrent uveitis, an autoimmune inflammatory disease in horses leading to breakdown of the outer blood-retinal barrier and influx of autoreactive T-cells into affected horses' vitrei. (mdpi.com)
  • As we believe that initiating events, leading to the breakdown of the outer blood-retinal barrier, take place at the cell surface of RPE cells as a particularly exposed barrier structure, this differential characterization of cell surface proteomes of native and cultured equine RPE cells is a prerequisite for future studies. (mdpi.com)
  • vitro
  • 9. Hjelle JT, Baird-Lambert J, Cardinale G, Specor S, Udenfriend S. Isolated microvessels: the blood-brain barrier in vitro. (springer.com)
  • These results validate the proposed microfluidic device with microgrooves as a promising in vitro tool for the compartmentalization and monitoring of barrier tissues. (rsc.org)
  • molecules
  • Yudilevich DL, DeRose N: Blood-brain transfer of glucose and other molecules measured by rapid indicator dilution. (springer.com)
  • vessel
  • No significant difference was found for measures of baseline retinal thickness or summarized retinal trunk vessel diameters. (arvojournals.org)
  • 4 5 Also of interest is the relation between changes in retinal vessel diameters and retinal thickening. (arvojournals.org)
  • Following the same eye, sub-retinal fluid accumulation was seen by OCT as vessel dilation resolved. (arvojournals.org)
  • He and colleagues figured that if the p53 protein was responsible for the positive switch, then blocking it in mice would halt the transition from scar-forming cells to blood vessel-forming cells. (medicalxpress.com)
  • macular
  • DME is a consequence of DR in the macular area and is secondary to retinal barrier rupture, which is in turn secondary to a range of metabolic changes brought about by hyperglycemia [ 4 ]. (hindawi.com)
  • disease
  • Plasticity of the blood-retinal barrier in disease. (arvojournals.org)
  • We suggest that the rat urethane retinopathy models the plasticity of BRB components--RPE and endothelia--over the course of retinal disease. (arvojournals.org)
  • Despite this intricate relationship, retinal neurodegeneration is a critical endpoint and neuroprotection, itself, can be considered a therapeutic target, independently of its potential impact on microvascular disease. (springer.com)
  • microfluidic
  • This research team developed a streamlined solution - a microfluidic chip that contains the cells present at the blood-retinal barrier, in a unique architecture that allows cellular communication and mechanical stimuli. (medgadget.com)
  • His lab is fabricating microfluidic, or "lab-on-a-chip," devices-chips as small as a dime that efficiently sort cells, separating out those of interest from a sample of blood or biological fluid. (medicalxpress.com)
  • inhibitor
  • Lastly, MLCK inhibitor also hastened barrier function, suggesting a critical need to regulate the acto-myosin cytoskeleton during BRB formation. (arvojournals.org)
  • cells
  • 14. Takata K, Kasahara T, Kasahara M, Ezaki O, Hirano H. Ultracytochemical localization of the erythrocyte/HepG2-type glucose transporter (GLUT1) in cells of the blood-retinal barrier in the rat. (springer.com)
  • Here, we studied ZIKV interactions with human retinal cells and evaluated ZIKV's pathobiology in mouse eyes. (jci.org)
  • Collectively, our study provides the first evidence to our knowledge that ZIKV causes retinal lesions and infects the cells lining the BRB and that ISG15 plays a role in retinal innate defense against ZIKV infection. (jci.org)
  • Then, human monocytic cells, THP-1, infected with Toxoplasma gondii or their conditioned medium were treated and the barrier function was evaluated by measurement of transepithelial electrical resistance (TEER) and immunocytochemistry of tight junction proteins. (arvojournals.org)
  • The purpose of this study was to characterize the cell surface proteome of native compared to cultured equine retinal pigment epithelium (RPE) cells. (mdpi.com)
  • A new discovery from the University of Virginia School of Medicine has revealed an unknown clockwork mechanism within the body that controls the creation of oxygen-carrying red blood cells. (medicalxpress.com)
  • Hematopoietic stem cells (HSCs) have long been regarded as the granddaddy of all blood cells. (medicalxpress.com)
  • The device is a relatively simple, passive cell-sorter that Karnik says may efficiently sort out material such as white blood cells-cells that may quickly be counted to identify conditions such as sepsis and inflammation. (medicalxpress.com)
  • protein
  • 1 - 2 , 4 Recently, the secreted protein Norrin (NDP) signaling, through a receptor complex composed of Frizzled-4 (FZD4), LRP5, and Tetraspanin-12 (TSPAN12), has been shown to direct retinal angiogenesis during development in both mice and humans. (arvojournals.org)
  • Among the latter were two RPE markers with highly specialized RPE functions: cellular retinaldehyde-binding protein (CRALBP) and retinal pigment epithelium-specific protein 65kDa (RPE65). (mdpi.com)
  • cell
  • Interestingly, ISG15 KO eyes exhibited severe chorioretinitis, which coincided with increased retinal cell death and higher ZIKV replication. (jci.org)