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  • transplant
  • Patients will receive a novel conditioning regimen with Busulfan, Cytoxan and Fludarabine (Bu/Cy/Flu) and either Alemtuzumab (Campath 1H) for recipients of a MUD or rabbit Antithymocyte Globulin (rATG) for recipients of unrelated UCB prior to hematopoietic stem cell transplant (HSCT). (clinicaltrials.gov)
  • HSPCs
  • This is because in RUNX1 knockout mice, primitive erythrocytes displayed a defective morphology and the size of blast cell population was substantially reduced, apart from the absence of HSPCs which would result in embryonic lethality by Embryonic day (E) 11.5 - 12.5. (wikipedia.org)
  • repopulation
  • This view was first challenged by the 2002 discovery by the Muller-Sieburg group in San Diego, who illustrated that different stem cells can show distinct repopulation patterns that are epigenetically predetermined intrinsic properties of clonal Thy-1lo Sca-1+ lin− c-kit+ HSC. (wikipedia.org)
  • microenvironment
  • Such efforts require a deeper understanding of the cell biology of HSC and HPC, the microenvironment that nurtures these cells, and greater mechanistic insight into cell surface receptors and intracellular signaling pathways regulating HSC/HPC function. (stembook.org)
  • progenitor cell
  • Caution is clearly required for non HSC/HPC stem/progenitor cell uses of CB, based on insufficient biological and preclinical information for MSC, EPC, and iPSC. (stembook.org)
  • yolk
  • Samokhvalov IM, Samokhvalova NI, Nishikawa S-I (2007) Cell tracing shows the contribution of the yolk sac to adult haematopoiesis. (springer.com)
  • In developing embryos, blood formation occurs in aggregates of blood cells in the yolk sac, called blood islands. (wikipedia.org)
  • Vogt used small chips of agar impregnated with a vital dye, (such as Nile Blue or Nile Red) which he placed on a particular cell or population of cells in Xenopus embryos until the dye absorbed into the yolk platelets within the desired cell(s). (wikipedia.org)
  • Cryopreservation
  • Here, we assess if the cells can further respond to additional cytokine stimulation and if function is maintained post cryopreservation. (ucl.ac.uk)
  • developmental
  • Mysm1 −/− p53 −/− mouse characterization showed a full rescue of Mysm1 −/− developmental and hematopoietic defects. (bloodjournal.org)
  • In developmental biology, fate mapping is a method of understanding the embryonic origin of various tissues in the adult organism by establishing the correspondence between individual cells (or groups of cells) at one stage of development, and their progeny at later stages of development. (wikipedia.org)
  • The disadvantage of this technique was that observation of single points in developmental time provide only snapshots of what cell movements are actually occurring and what fates are being assigned. (wikipedia.org)
  • Seth Blair, also in Stent's lab, introduced a novel ablation technique that could be used in tandem with lineage tracing to pursue the questions relating to developmental potential changes in cell fate in experimentally perturbed embryos that were first raised by Roux and Driesch (1980). (wikipedia.org)
  • lineage
  • 1 , 2 The ETS family transcription factor PU.1 ( Spi-1 ) is one of the most important regulators of hematopoietic lineage development. (bloodjournal.org)
  • 5 , 6 PU.1 expression at these early hematopoietic stages may be due to "lineage priming," 7 which presumably represents a selective open chromatin form characteristic of early oligopotent stages. (bloodjournal.org)
  • 13 - 17 Nonetheless, PU.1 can play an instructive role in hematopoietic lineage decisions. (bloodjournal.org)
  • The lymphoid lineage is primarily composed of T-cells and B-cells (types of white blood cells). (wikipedia.org)
  • When carried out at single-cell resolution, this process is termed cell lineage tracing. (wikipedia.org)
  • Genetic cell lineage tracing became an extremely powerful approach when Nat Sternberg and Daniel Hamilton identified a topoisomerase in P1 bacteriophage called Cre recombinase, which has site-specific (loxP site) recombination activity (1981). (wikipedia.org)
  • malignant
  • Natural Killer (NK) cells are effective at targeting malignant cells without being direct effectors of graft-versus-host disease. (ucl.ac.uk)
  • distinct
  • This finding revealed a new function for regulatory T cells that is distinct from their role in immunomodulation[disambiguation needed]. (wikipedia.org)
  • The information Vogt gathered from his tracing experiments of distinct cells and populations of cells in Xenopus was then pooled to construct a fate map. (wikipedia.org)
  • CD34
  • CD34 + cells were isolated using immuno-magnetic beads and cultured for up to 7 days. (scielo.br)
  • On the other hand, the fraction of CD34 + CD38 - cells became 52.0 ± 29% of all CD34 + cells. (scielo.br)
  • The absolute number of CD34 + CD38 - cells was expanded on average 15 ± 12-fold when CD34 + cells were cultured with TPO + FL + KL for 7 days. (scielo.br)
  • We conclude that culture of CD34 + cells with TPO + FL + KL results in a significant increase in the number of candidate stem cells with the CD34 + CD38 - phenotype. (scielo.br)
  • About 1% of bone marrow cells express CD34, and generally less than 1% of these cells are CD38-negative. (scielo.br)
  • Pecora et al discovered that patients who had received more than two cycles of chemotherapy and were shown to have "chemotherapy-resistant NHL mobilized a significantly lower percentage of CD34+CD33− cells than did chemotherapy-sensitive NHL patients. (wikipedia.org)
  • They also found the reverse that those who had received less significant levels of chemotherapy treatment had higher levels of CD34+CD33− cells. (wikipedia.org)
  • In a small human clinical trial to evaluate the safety and efficacy of fucoidan ingestion (brown seaweed extract), 3g daily of 75% w/w oral fucoidan for 12 days increased the proportion of CD34+CXCR4+ from 45 to 90% and the serum SDF-1 levels, which could be useful in CD34+ cells homing/mobilization via SDF-1/CXCR4 axis. (wikipedia.org)
  • tumor
  • Nov overexpression results in reduced tumor size in glioma cells xenografts, but enhances metastatic potential in xenotransplanted melanoma cells. (wikipedia.org)
  • Another explanation of this observation is provided by a study that shows the ability of CXCL12 (and CCL2) producing tumors to entrain neutrophils that inhibit seeding of tumor cells in the lung. (wikipedia.org)
  • embryonic development
  • However, in terms of the actual terminology, in 1909 Alexander A. Maximow wrote in German of "Stammzelle" for the same concept in his paper "The lymphocyte as a stem cell, common to different blood elements in embryonic development and during the post-fetal life of mammals" (English translation). (wikipedia.org)
  • haematopoiesis
  • In some vertebrates, haematopoiesis can occur wherever there is a loose stroma of connective tissue and slow blood supply, such as the gut, spleen or kidney. (wikipedia.org)
  • clonal
  • As the HUMARA assay is based on the epigenetic state of cells, the underlying genetic determinants of the clonal expansion remained to be uncovered. (wikipedia.org)
  • embryo
  • North TE, de Bruijn MFTR, Stacy T, Talebian L, Lind E, Robin C, Binder M, Dzierzak E, Speck NA (2002) Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. (springer.com)
  • Müller AM, Medvinsky A, Strouboulis J, Grosveld F, Dzierzak E (1994) Development of hematopoietic stem cell activity in the mouse embryo. (springer.com)
  • The 46 chromosomes undergo changes prior to the meiotic division which leads to the formation of the embryo having two cells. (wikipedia.org)
  • Once the cells were effectively labeled, the agar chip could be removed and the embryo was allowed to develop normally. (wikipedia.org)
  • Although innovative for the time, this technique is limiting in that the size of a chip of agar may not accommodate single-cell resolution studies at later stages of development, since successive cell divisions will yield smaller cells (until the embryo develops into a larval form that can eat, and thereby grow larger). (wikipedia.org)
  • Additionally, the cell or cell population of interest must be superficial, since the agar chip with the dye must be placed on the surface of the embryo. (wikipedia.org)
  • Genetic
  • Patients with RD have a genetic defect that affects the T cells and at least one other type of immune cell. (wikipedia.org)
  • The genetic material of the sperm and egg then combine to form a single cell called a zygote and the germinal stage of prenatal development commences. (wikipedia.org)
  • Fertilization takes place when the spermatozoon has successfully entered the ovum and the two sets of genetic material carried by the gametes fuse together, resulting in the zygote (a single diploid cell). (wikipedia.org)
  • neutrophils
  • Filgrastim, sold under the brand name Neupogen among others, is a medication used to treat low blood neutrophils due to a number of causes. (wikipedia.org)
  • gene expression
  • The precise nature of the elements of gene expression that contribute to stem cell characteristics have been assessed by transcriptional profiling and the elements of a stem cell signature refined via comparative expression analyses. (bloodjournal.org)
  • Gene silencing is the regulation of gene expression in a cell to prevent the expression of a certain gene. (wikipedia.org)
  • RNA interference (RNAi) is a natural process used by cells to regulate gene expression. (wikipedia.org)