Blood disseminated tumor cells immunomagnetic. Medical search. Frequent questions

The detection of CTCs in peripheral blood is one of the most promising approaches to identify disseminated disease in colorectal cancer (CRC). (researchsquare.com)
The isolation and molecular analysis of CTCs in peripheral blood is one of the most promising approaches to identify disseminated disease, in order to upgrade or downgrade the multimodal therapy. (researchsquare.com)
Background: Circulating tumour cells (CTCs) are cells that have detached from a primary tumour, circulate in the peripheral blood, and are considered to be the main root of distant metastases. (iiarjournals.org)
Even then he presumed that these cells, today known as circulating tumour cells (CTCs), could play a role in the development of distant metastases. (iiarjournals.org)
Nowadays, a huge body of literature describes CTCs as cells that, after detaching from the primary tumour and circulating through the blood or lymphatic system, are the main cause of the occurrence of metastases in patients with epithelial cancer ( 2 - 4 ). (iiarjournals.org)
Numerous studies suggest that detection of CTCs in the blood or disseminated tumour cells (DTCs) in the bone marrow of patients with cancer is linked to poor outcomes of the disease and reduced treatment efficiency ( 5 , 6 ). (iiarjournals.org)
There are several different methods for the detection of DTCs and CTCs from bone marrow or blood ( 7 - 9 ). (iiarjournals.org)
Currently, only one methodology for the discovery and enumeration of CTCs is approved, at least for the metastatic setting by the US Food and Drug Administration (FDA), namely the so-called Cell Search® System ( 10 ). (iiarjournals.org)
This method is based on the immunomagnetic enrichment of CTCs from patient's blood samples, with subsequent staining of these cells. (iiarjournals.org)
Cancer metastases descend from proliferative circulating tumor cells (CTCs) which disseminate from the primary tumor via blood and lymph vessels. (fraunhofer.de)
Nonetheless the small number of CTCs compared to billions of blood cells in a patient sample requires sensitive methods to obtain reproducible tumor cell recovery rates. (fraunhofer.de)
Circulating tumor cells (CTCs), as one of the materials of "liquid biopsy, " have important guiding roles in the early diagnosis of head and neck squamous cell carcinoma, judgment of prognosis and therapeutic response, and monitoring of tumor recurrence and metastasis. (sdu.edu.cn)
focus on circulating tumor cells (CTCs)[J]. Oral Oncol, 2017, 74: 83- 89. (sdu.edu.cn)
An emerging method to detect metastasis is the analysis of circulating tumor cells (CTCs), which has shown promise in filling the gaps left by other diagnostic methods. (iiarjournals.org)
CTCs are tumor cells originating from primary sites or metastases that circulate in the patients' bloodstream and are very rarely found in healthy individuals ( 9 , 10 ) ( Figure 1 ). (iiarjournals.org)
Morphological-based isolation separates CTCs according to size discrepancies, using isolation by size of epithelial tumor cells (ISET) or according to density, using density-gradient separation ( 4 ). (iiarjournals.org)
The enumeration of circulating tumor cells (CTCs) represents an effective prognostic and predictive biomarker, which is able to monitor efficacy of adjuvant therapies, detect early development of (micro)metastases and at last, assess therapeutic responses of advanced disease earlier than traditional imaging methods. (amegroups.org)
Moreover, since repeated tissue biopsies are invasive, costly and not always feasible, the assessment of tumor characteristics on CTCs, by a peripheral blood sample as a 'liquid biopsy', represents an attractive opportunity. (amegroups.org)
In the last decade, the detection of disseminated tumor cells (DTCs) in the bone marrow and circulating tumor cells (CTCs) in the blood have demonstrated to provide useful information for the clinical management of BC by predicting treatment benefit earlier than traditional imaging methods. (amegroups.org)
Prospective studies have demonstrated the effectiveness of identifying and quantifying CTCs in the prediction of malignant tumour treatment outcomes, including cancers of the breast, colon and prostate (3). (czytelniamedyczna.pl)
CTCs can be isolated from patients' blood in the course of most cancers due to the ease of identification of the epithelial phenotype of the cancer. (czytelniamedyczna.pl)
CTCs are suspected of being instrumental for dissemination of the cancer during surgery, which involves mechanical manipulation and traumatisation of the primary tumour (5). (czytelniamedyczna.pl)

Stiefel's thesis deals with detecting tumor features based on the automated isolation of circulating tumor cells from liquid biopsies and thus contributes significantly to establishing a platform technology of Fraunhofer IMM in applied research of liquid biopsy. (fraunhofer.de)
The defense of his dissertation on "Automated Isolation of Circulating Tumor Cells and their Molecular Biological Analysis" took place on January 30, 2023 at the Johannes Gutenberg University Mainz. (fraunhofer.de)
In doing so, CTCelect enabled automated dispensing of single circulating tumor cells from HNSCC patient samples, qPCR-based confirmation of tumor-related biomarkers with a simplified one-step protocol and immunostaining using a panel of tumor markers. (fraunhofer.de)
Progress in clinical research on circulating tumor cells in squamous cell carcinoma of the head and neck[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 18-24. (sdu.edu.cn)
Significance of circulating tumor cells in patients with squamous cell carcinoma of the head and neck: initial results[J]. Arch Otolaryngol Head Neck Surg, 2010, 136 (12): 1274- 1279. (sdu.edu.cn)
Evaluation of incidence, significance, and prognostic role of circulating tumor microemboli and transforming growth factor-β receptor I in head and neck cancer[J]. Head Neck, 2017, 39 (11): 2283- 2292. (sdu.edu.cn)
Circulating tumour cells in locally advanced head and neck cancer: preliminary report about their possible role in predicting response to non-surgical treatment and survival[J]. Eur J Cancer, 2012, 48 (16): 3019- 3026. (sdu.edu.cn)
A simple multicolor flow cytometry protocol for detection and molecular characterization of circulating tumor cells in epithelial cancers[J]. Cytometry A, 2012, 81 (6): 489- 495. (sdu.edu.cn)
Monitoring of circulating tumor cells and their expression of EGFR/phospho-EGFR during combined radiotherapy regimens in locally advanced squamous cell carcinoma of the head and neck[J]. Int J Radiat Oncol Biol Phys, 2012, 83 (5): e685- e690. (sdu.edu.cn)
Significance of circulating tumor cell detection using the CellSearch system in patients with locally advanced head and neck squamous cell carcinoma[J]. Eur Arch Otorhinolaryngol, 2013, 270 (10): 2745- 2749. (sdu.edu.cn)
Emerging techniques using the analysis of circulating tumor cells show promising results in predicting and identifying the early stages of breast cancer metastasis in patients. (iiarjournals.org)
Circulating tumor cells in the blood stream. (iiarjournals.org)
More recently, other potential blood-based markers are emerging as independent parameters for prediction development and outcome in metastatic disease, including circulating tumor microemboli and circulating tumor materials (CTMat). (amegroups.org)
This is a current review on the circulating tumour cells (CTC) in patients with renal cell carcinoma as a potential diagnostic tool that will allow for more accurate assessment of advanced disease and the monitoring of treatment effects. (czytelniamedyczna.pl)
Circulating Tumour Cells (CTC) are a population of cells that have detached from the tumour mass and are able to disseminate via the vascular system and create metastatic foci in the body (1). (czytelniamedyczna.pl)
Initially, the immune system eliminates the circulating cancer cells from the bloodstream, but over time, the cells remain there for longer periods and become identifiable. (czytelniamedyczna.pl)
The first published report of circulating tumour cells dates back to 1869 (2). (czytelniamedyczna.pl)
The circulating cells acquire properties of mesechymal cells, i.e. they are able to function independently. (czytelniamedyczna.pl)
demonstrated that 80% of circulating cells of prostate or breast cancer express mesenchymal and epithelial markers (8). (czytelniamedyczna.pl)
Circulating cancer cells are gradually gaining importance in clinical medicine. (czytelniamedyczna.pl)
The presence of high expressing epithelial cell adhesion molecule (EpCAM high ) circulating tumor cells (CTC) enumerated by CellSearch ® in blood of cancer patients is strongly associated with poor prognosis. (oncotarget.com)

In the last two decades, CTC analysis from blood samples was established for diagnostic and prognostic purposes and therapy monitoring in the course of liquid biopsy. (fraunhofer.de)
The aim of this thesis was to realize single cell isolation by means of automated immunomagnetic bead enrichment and antibody-based fluorescence detection in a chip-based microfluidic system and the molecular biological characterization of these cells to assay tumor features from liquid biopsy. (fraunhofer.de)
Liquid biopsy: an emerging prognostic and predictive tool in head and neck squamous cell carcinoma (HNSCC). (sdu.edu.cn)
The introduction of "liquid biopsy", for screening and treatment monitoring, forms a very attractive outlook, where cases could be easily found by a blood test. (czytelniamedyczna.pl)

In a prototype enrichment device (IsoMAG), we established an isolation protocol with EpCAM-targeting 1 µm magnetic beads for automated enrichment tested on different epithelial cancer cell lines. (fraunhofer.de)
Epithelial cells in the peripheral blood of patients with cancer of the head and neck: incidence, detection and possible clinical significance[J]. Radiother Oncol, 2001, 59 (2): 213- 217. (sdu.edu.cn)
This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)

OBJECTIVE: The effects of intraoperative blood salvage (IBS) on time to tumor recurrence, disease-free survival and overall survival in hepatocellular carcinoma (HCC) patients undergoing liver transplantation were assessed to evaluate the safety of IBS. (bvsalud.org)

Vaccination with tumor lysate-pulsed dendritic cells elicits antigen-specific, cytotoxic T-cells in patients with malignant glioma. (nwbio.com)
However, the safety of IBS during liver transplantation for patients with HCC is questioned due to fear of disseminating malignant cells. (bvsalud.org)

The presence of such cells in the blood coexists with micrometastases, which are clinically silent, and/or precedes the clinical emergence of metastases (4). (czytelniamedyczna.pl)

However, in some cases, selective CD4+ T cell tolerance provides a unique therapeutic opportunity to maximize self antigen-targeted immune and antitumor responses without inducing autoimmunity by incorporating self antigen-independent CD4+ T cell epitopes into cancer vaccines. (wikipedia.org)
Because tumour dormancy provides a unique therapeutic window to prevent metastatic disease, we discuss on-going clinical trials and weigh the potential for immunotherapy to eradicate dormant disease. (nature.com)

Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)
6 ) emphasized that the inclusion of serum tumor markers is an important factor in the postoperative monitoring of breast cancer patients ( 7 , 8 ). (iiarjournals.org)

One example is antigen-specific CD4+ T cell tolerance, which serves as the primary mechanism restricting immunotherapeutic responses to the endogenous self antigen guanylyl cyclase c (GUCY2C) in colorectal cancer. (wikipedia.org)

Therefore, this work compares manual and automated immunomagnetic enrichment in terms of cell recovery in dependency of cell line and input number, bead characteristics, biofunctionaliza-tion protocol and blood cell contamination focusing on head and neck squamous cell carcinoma (HNSCC) and metastatic breast cancer. (fraunhofer.de)

The easier access for multiple sampling from peripheral blood makes it the method of choice. (iiarjournals.org)

The majority of deaths from breast cancer are not due to the primary tumor itself, but are the result of metastasis to other organs in the body ( 2 ). (iiarjournals.org)

CD4 + T cells contribute to the remodeling of the microenvironment required for sustained tumor regression upon oncogene inactivation. (nature.com)
Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. (mdpi.com)

Banchereau J, Ueno H, Dhodapkar M, Connolly J, Finholt JP, Klechevsky E, Blanck JP, Johnston DA, Palucka AK, Fay J. Immune and clinical outcomes in patients with stage IV melanoma vaccinated with peptide-pulsed dendritic cells derived from CD34+ progenitors and activated with type I interferon. (nwbio.com)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

Conclusion: By this methodology, cells from mammary carcinoma cell lines can be detected in blood samples. (iiarjournals.org)
In a mammary carcinoma model, depletion of CD25+ regulatory T cells increase the amount of CD8+CD11c+PD110, which target and kill the tumors. (wikipedia.org)
Persistence of solitary mammary carcinoma cells in a secondary site: a possible contributor to dormancy. (nature.com)

In the microfluidic benchtop platform CTCelect, we characterized each process step with two different carcinoma cell lines (MCF-7, SCL-1) demon-strating up to 87 Percent enrichment, 73 percent optical detection and dispensing efficiency. (fraunhofer.de)
Detection of rare disseminated tumor cells identifies head and neck cancer patients at risk of treatment failure[J]. Clin Cancer Res, 2003, 9 (14): 5287- 5294. (sdu.edu.cn)
Parasite clearance following treatment with antimalarial drugs resulted in decreased detection of HIV-1 particles derived from the CD14 macrophage cell subset and correlated with a marked diminution in systemic immune activation. (lww.com)

Phase I/II study of vaccination with electrofused allogeneic dendritic cells/autologous tumor-derived cells in patients with stage IV renal cell carcinoma. (nwbio.com)
BACKGROUND: IBS is highly effective to reduce the use of allogeneic blood transfusion. (bvsalud.org)

Relationship between hematogenous tumor cell dissemination and cellular immunity in DCIS patients. (nature.com)
The dissemination of tumor cells from the primary tumor to distant sites through bloodstream cannot be early detected by standard imaging methods. (amegroups.org)

Goss, P. E. & Chambers, A. F. Does tumour dormancy offer a therapeutic target? (nature.com)
Folkman, J. Tumor angiogenesis: therapeutic implications. (nature.com)

Materials and Methods: Blood samples of a healthy donor were mixed with specific numbers of cells from different breast carcinoma cell line cells. (iiarjournals.org)
T cell depletion methods can be broadly categorized into either physical or immunological. (wikipedia.org)
Immunological methods utilize antibodies, either alone, in conjunction with homologous, heterologous, or rabbit complement factors which are directed against the T cells. (wikipedia.org)
Currently, ex vivo techniques most commonly employ positive or negative selection methods using immunomagnetic separation. (wikipedia.org)
Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)

As early as 1869, cells with the morphology of cancer cells had been found by Thomas Ashworth in the blood of a person who died from metastatic cancer ( 1 ). (iiarjournals.org)

Against this background, the Fraunhofer Institute for Microengineering and Microsystems has recognized the unmet need of standardized CTC isolation and developed automated microfluidic platforms to enrich, detect and isolate single tumor cells from 7.5 mL whole blood. (fraunhofer.de)
40 to 56.7 percent of cells were recovered after complete isolation from 7.5 mL untreated whole blood. (fraunhofer.de)
We highlighted the bottlenecks of microfluidic cell isolation in dependency of bead-binding on the cell surface and implemented a gating function for the cytometer subunit to selectively dispense cells instead of autofluorescent objects. (fraunhofer.de)

The primary cause of tumor-related death in breast cancer (BC) is still represented by distant metastasization. (amegroups.org)
As a result of changes in their adhesion molecules, the cells separate from the primary cell population and gain the ability to migrate independently within the extracellular matrix and, eventually, outside it (7). (czytelniamedyczna.pl)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

Currently, intensive research is conducted on the importance of CTC in the biology and therapy of renal cell carcinoma (RCC) and transitional cell carcinoma (TCC). (czytelniamedyczna.pl)
Dendritic cell immunotherapy for patients with metastatic renal cell carcinoma: University of Tokyo experience. (nwbio.com)
Bleumer I, Tiemessen DM, Oosterwijk-Wakka JC, Völler MC, De Weijer K, Mulders PF, Oosterwijk E. Preliminary analysis of patients with progressive renal cell carcinoma vaccinated with CA9-peptide-pulsed mature dendritic cells. (nwbio.com)

Fusion cell vaccination of patients with metastatic breast and renal cancer induces immunological and clinical responses. (nwbio.com)

Baseline characteristics were similar between groups (sex, Charlson Comorbidity score, tumor entity). (bvsalud.org)

Ex vivo techniques enable a more accurate count of the T cells in a graft and also has the option to 'addback' a set number of T cells if necessary. (wikipedia.org)
In contrast, in-vivo TCD is performed using anti-T cell antibodies or, most recently, post-HSCT cyclophosphamide. (wikipedia.org)
In haploidentical hematopoietic stem cell transplantation, in vivo TCD suppressed lymphocytes early on. (wikipedia.org)

Examples of physical separation include using counterflow centrifugal elutriation, fractionation on density gradients, or the differential agglutination with lectins followed by rosetting with sheep red blood cells. (wikipedia.org)

Immunomagnetic HIV-1 capture analysis was used to determine the cellular origin of cell-free virus particles present in all 30 plasma samples and indices of immune activation were measured using enzyme-linked immunosorbent assays. (lww.com)
Babatz J, Röllig C, Löbel B, Folprecht G, Haack M, Günther H, Köhne CH, Ehninger G, Schmitz M, Bornhäuser M. Induction of cellular immune responses against carcinoembryonic antigen in patients with metastatic tumors after vaccination with altered peptide ligand-loaded dendritic cells. (nwbio.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Antiretroviral therapy, the most common treatment for patients with HIV, has been shown to restore CD4+ T cell counts. (wikipedia.org)

Maturation and trafficking of monocyte-derived dendritic cells in monkeys: implications for dendritic cell-based vaccines. (nwbio.com)

CONCLUSION: Dissection with tungsten needle electrodes and PTFE-coated spatula electrodes appears an effective surgical blood-saving measure without increased risk of wound healing disorders. (bvsalud.org)

Vaccination of prostatectomized prostate cancer patients in biochemical relapse, with autologous dendritic cells pulsed with recombinant human PSA. (nwbio.com)

Dendritic cells acquire antigen from apoptotic cells and induce class I-restricted CTLs. (nwbio.com)

Intraoperative cell salvage while widely used still poses significant risks and hazards due to human error. (bvsalud.org)

Early on it was apparent that TCD was good for preventing GVHD, but also led to increased graft rejection, this problem can be solved by transplanting more hematopoietic stem cells. (wikipedia.org)
This procedure is called 'megadose transplantation' and it prevents rejection because the stem cells have an ability (i.e. veto cell killing) to protect themselves from the host's immune system. (wikipedia.org)

In this regard, we used blood samples from a healthy donor mixed with different numbers of established breast cancer cells (CAMA-1, MCF7, ZR-75-1) and carried out subsequent gene expression analysis for cytokeratins 8, 18 and 19. (iiarjournals.org)

T-cell depletion (TCD) is the process of T cell removal or reduction, which alters the immune system and its responses. (wikipedia.org)
Although TCD is beneficial to prevent GVHD there are some problems it can cause a delay in recovery of the immune system of the transplanted individual and a decreased Graft-versus-tumor effect. (wikipedia.org)
This problem is partially answered by more selective depletion, such as depletion of CD3+ or αβT-cell and CD19 B cell, which preserves other important cells of the immune system. (wikipedia.org)
However, over time, an individual's immune system can no longer continue to replace CD4+ T cells. (wikipedia.org)

White blood cell contamination, usually hampering further downstream analysis of enriched tumor cells, was measured by FACS and minimized to about 1200 cells starting from 7.5 mL blood. (fraunhofer.de)

Indeed, antigen-presenting cells are important reservoirs of HIV-1 [ 16,17 ] and induction of HIV-1 replication within these cells may contribute significantly to the cofactor effect of confections on HIV-1 pathogenesis. (lww.com)

Experiments show that transplantation of other types of veto cells along with megadose haploidentical HSCT allows to reduce the toxicity of the conditioning regimen, which makes this treatment much safer and more applicable to many diseases. (wikipedia.org)

Klein, G. Foulds' dangerous idea revisited: the multistep development of tumors 40 years later. (nature.com)

Another method is addition of cells back into the graft, after a comprehensive TCD method, examples are re-introduction of natural killer cells (NK), γδ T-cells and T regulatory cells (Tregs). (wikipedia.org)

INTRODUCTION: Resection of musculoskeletal tumors and reconstruction with tumor endoprostheses often results in blood loss requiring transfusion of blood products. (bvsalud.org)

Dormant cancer cells: programmed quiescence, senescence, or both? (nature.com)

Anatomy16

Organisms12

Diseases49

Chemicals and Drugs30

Analytical, Diagnostic and Therapeutic Techniques and Equipment50

Phenomena and Processes27

Disciplines and Occupations4

Technology, Industry, Agriculture5

Information Science2

Health Care6

CTCs22

Circulating21

Liquid biopsy4

Epithelial3

Recurrence1

Malignant2

Metastases1

Provides a unique therapeutic2

Markers2

Colorectal cancer1

Head and neck squamous cell carc1

Peripheral blood1

Metastasis1

Regression2

Melanoma2

Mammary carcinoma3

Detection3

Allogeneic2

Dissemination2

Therapeutic2

Methods5

18691

Isolation3

Primary3

Renal cell carc3

Clinical1

Characteristics1

Vivo3

Separation1

Cellular3

Counts1

Implications1

Conclusion1

Patients1

Acquire1

Widely1

Stem cells2

Breast cancer1

Immune system4

Analysis1

Significantly1

Treatment1

Development1

Method1

Introduction1

Cancer cells1