When the inability of male transgenic rats to transmit the transgene is not associated with mosaicism, microinsemination techniques such as intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) can be used for passage of the transgenes. (go.jp)
Our ICSI procedure includes the use of a piezo-driven small-sized injection pipette (2-4 μm in diameter), isolated sperm heads, and freshly ovulated oocytes. (go.jp)
Studies that compared postnatal outcomes between in vitro-conceived (IVF, ICSI or IVM) and in vivo-conceived mammalian animal models were included. (bvsalud.org)
Fertilization2
Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
SECM technique may be a valuable tool for accurately assessing the quality of embryos and thereby contribute to improving outcomes associated with assisted reproduction, including human in vitro fertilization. (bioone.org)
Outcomes1
WIDER IMPLICATIONS: Conception via in vitro or in vivo means appears to have an influence on measurable outcomes of offspring physiology, manifesting differently across the species studied. (bvsalud.org)
Reproductive2
Therefore, in future, melatonin can be implicated as preferable therapeutic especially in IVF and assisted reproductive techniques. (jrhm.org)
The dormancy of primordial oocytes may be required to reserve the non-growing oocyte pool for the long reproductive life in mammals. (go.jp)
Growth2
Further, melatonin and its receptors play a significant role in antioxidant defense mechanism of ovary for follicular growth and maturation. (jrhm.org)
These quiescent primordial oocytes are activated, perhaps by certain mechanisms involving the interaction between stimulatory and inhibitory factors, to enter the growth phase. (go.jp)
Quality1
Since recent clinical studies have indicated that dehydroepiandrosterone (DHEA) supplementation may reverse the problem of oocyte quality, this review of the literature was undertaken in an attempt to find an explanation of why this is effective? (biomedcentral.com)
Transfer1
In vivo conception included mating, artificial insemination, or either of these followed by embryo transfer to a recipient animal with or without in vitro culture. (bvsalud.org)
Evidence2
Despite considerable heterogeneity, these studies suggest that the use of IVF or maturation results in offspring with higher birthweights and a longer length of gestation, with most of this evidence coming from studies in cattle. (bvsalud.org)
Today, as research techniques become more refined and the evidence generated more complex, silos of knowledge are generated and experts find it difficult to cross between sub-disciplines. (biomedcentral.com)