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  • limbs
  • In lower vertebrates the blastema is particularly important in the regeneration of severed limbs. (britannica.com)
  • Position-specific induction of ectopic limbs in non-regenerating blastemas on Axolotl forelimbs. (uci.edu)
  • cAMP and cGMP measured by RIA and observed by immunohis-tochemistry in unamputated limbs and in regenerating newt limbs at early- and medium-bud stages showed cAMP and cGMP cytoplasmic accumulations in blastemata and in stumps of the regenerating limbs, with increases up to 1900% over unamputated controls. (springer.com)
  • In blastemata the cAMP increase was greater than that of cGMP while in stumps of the regenerating limbs the increase of cGMP was of a much greater magnitude than that of cAMP. (springer.com)
  • These results indicate that positional memory in regenerating limbs is directly related to blastema cell affinity, and that very similar or identical sets of level-specific affinity properties are shared by forelimb and hindlimb cells. (biologists.org)
  • We have found that pseudotyped Maloney Murine Leukemia Virus (MMLV) retroviruses can very efficiently infect axolotl cells in vitro and in vivo , and can be used for both lineage and functional analyses in regenerating limbs. (biologists.org)
  • stump
  • The assay involved autografting or homografting late bud forelimb blastomas derived from the wrist, elbow or midupper arm levels to the dorsal surface of the blastema-stump junction of an ipsilateral, medium-bud-stage hindlimb regenerating from the midthigh level. (biologists.org)
  • cytoplasmic
  • Stage III was characterized by the decrease in size and number of various cytoplasmic constituents and was considered to represent cells in the state of regression. (docme.ru)
  • appendage
  • Blastema , also called Regeneration Bud , in zoology, a mass of undifferentiated cells that has the capability to develop into an organ or an appendage. (britannica.com)
  • Beginning with the formation of a cellular aggregation (reportedly a blastema), these lizards are able to develop a replacement appendage that, at least superficially, resembles the original, complete with nerves, blood vessels and skeletal support. (biomedcentral.com)
  • After the loss of an appendage undifferentiated, pluri- or multipotent cells from different origins accumulate at the damaged surface to form a regeneration blastema. (biomedcentral.com)
  • lower vertebrates
  • However, some lower vertebrates such as teleost fish and some amphibians are able to compensate for the loss of body parts by regenerating a nearly perfect copy of the original part by dedifferentiating cells in vivo to facilitate regeneration. (biomedcentral.com)
  • biology
  • Albeit a great deal is now known about the biology of these cells, most molecular data have come from cDNA and genomic analyses. (biomedcentral.com)
  • Annual Review of Cell and Developmental Biology 18: 515-539. (els.net)
  • expression
  • Unique gene expression profile of the proliferating Xenopus tadpole tail blastema cells deciphered by RNA-sequencing analysis. (jove.com)
  • In the present study, we used RNA-sequencing analysis to analyze the gene expression profile of isolated proliferating blastema cells of regenerating Xenopus laevis tadpole tails. (jove.com)
  • Viral gene transfer is an attractive alternative, as it can provide stable gene expression and can, in principle, be targeted to specific cell types based on the availability of a receptor. (biologists.org)
  • genetic
  • Sequencing of the Schmidtea mediterranea genome and some expressed sequence tag projects have generated extensive data on the genetic profile of these cells. (biomedcentral.com)
  • However, very few molecular tools exist for studying crucial cell lineage relationships over regeneration or for robust and sustained misexpression of genetic elements to test their function. (biologists.org)
  • tail
  • We used flow cytometry to isolate proliferating cells, and non-proliferating blastema cells, from regenerating tadpole tails as well as proliferating tail bud cells from tail bud embryos, the latter two of which were used as control cells, based on their DNA content. (jove.com)
  • Among them, whole mount in situ hybridization revealed that chromosome segregation 1-like and interleukin 11 were expressed in the broad area of the tail blastema, while brevican, lysyl oxidase, and keratin 18 were mainly expressed in the notochord bud in regenerating tails. (jove.com)
  • We further combined whole mount in situ hybridization with immunohistochemistry for the incorporated 5-bromo-2-deoxyuridine to confirm that keratin 18 and interleukin 11 were expressed in the proliferating tail blastema cells. (jove.com)
  • We show that tail loss is followed by a latent period of scar-free healing of the wound site, and that regeneration is blastema-mediated. (biomedcentral.com)
  • regenerate
  • Much less is known about the cells that are specialized to generate and regenerate the blueprint (the pattern-forming cells) in order to instruct the pattern-following cells as to how and where to rebuild the structures. (springer.com)
  • The grafted blastemas consistently displaced to their corresponding levels on the proximodistal axis of the host regenerate, indicating the existence of level-specific differences in blastema cell affinity. (biologists.org)