NHS Biotin is the simplest NHS ester activated biotinylation reagent for labeling primary amines such as protein lysines, The short spacer arm of NHS biotin allows membrane permeability. (soltecventures.com)
In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. (wikipedia.org)
Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). (wikipedia.org)
Most chemical biotinylation reagents consist of a reactive group attached via a linker to the valeric acid side chain of biotin. (wikipedia.org)
As the biotin binding pocket in avidin / streptavidin is buried beneath the protein surface, biotinylation reagents possessing a longer linker are desirable, as they enable the biotin molecule, once it has been attached to its target, to be more accessible to binding avidin/streptavidin/Neutravidin protein. (wikipedia.org)
Once tagged, the protein is then incubated with BirA allowing biotinylation to take place in the presence of biotin and ATP. (wikipedia.org)
ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology to enable reproducibility in your biotinylation process. (vectorlabs.com)
This reagent permits direct spectroscopic quantification of incorporated biotin. (vectorlabs.com)
For example, antibodies labeled with NHS-PEG4-Biotin exhibit less aggregation when stored in solution as compared to antibodies labeled with reagents having only hydrocarbon spacers. (thermofisher.com)
NHS-PEG12-Biotin is a very long (56.0Å), pegylated, water-soluble, NHS-ester biotinylation reagent to label amines and maximize solubility of antibodies and other proteins. (thermofisher.com)
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications. (thermofisher.com)
Varieties of biotin NHS-ester reagents differ in length, solubility, cell permeability and cleavability. (thermofisher.com)
LGC, Biosearch Technologies offers 3', internal and 5' biotinylation reagents, as well as biotin derivatives and DNP phosphoramidites, with various spacers for incorporation into oligos. (biosearchtech.com)
Biotin-dPEG ® 11 -azide, product number 10784, is part of a family of biotinylation products designed for use in azide-alkyne click chemistry reactions. (quantabiodesign.com)
Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or a surface such as glass or gold. (quantabiodesign.com)
Biotinylation is the process of covalently attaching biotin to molecules (such as amino acids, peptides, antibodies, or proteins). (newyorktimesnow.com)
Due to the small molecular weight of biotin, biotinylation is usually rapid and specific and is unlikely to interfere with the inherent function of the molecule. (newyorktimesnow.com)
NHS-LC-Biotin, Succinimidyl 6-(biotinamido)hexanoate, is an amino-reactive biotinylation reagent with a long spacer arm. (proteochem.com)
The sCIP method is enabled by a high yielding and scalable route to dialkoxydiphenylsilane fluorenylmethyloxycarbonyl (DADPS-Fmoc)-protected amino acid building blocks, which enable the facile synthesis of customizable, isotopically labeled, and chemically cleavable biotin capture reagents. (bvsalud.org)
Biotinylation is the addition of a biotin group to the terminus or side chain of a peptide to allow for binding to avidin and streptavidin. (nridigital.com)
Peptides2
Guided by our empirical comparison of fragmentation patterns of six CBCC reagent combinations, we achieved enhanced coverage of cysteine-labeled peptides. (bvsalud.org)
M imotopes provides custom peptide synthesis, catalogue peptides, PepSet™ peptide libraries, reagents, antibodies, and an array of other immunology services for the research market. (nridigital.com)
Bioscience2
Human IgG antibody Laboratories manufactures the jena bioscience gmbh ps-101 reagents distributed by Genprice. (nfkb-p65.info)
The Jena Bioscience Gmbh Ps-101 reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (nfkb-p65.info)
Linkers1
linkers that incorporate poly(ethylene) glycol (PEG) can make water-insoluble reagents soluble or increase the solubility of biotinylation reagents that are already soluble to some extent. (wikipedia.org)
Solubility4
Amine-reactive biotinylation reagents can be divided into two groups based on water solubility. (wikipedia.org)
The water solubility of sulfo-NHS-esters stems from their sulfonate group on the N-hydroxysuccinimide ring and eliminates the need to dissolve the reagent in an organic solvent. (wikipedia.org)
The solubility of biotinylation reagents will largely affect the labeling ability of proteins or other macromolecules. (newyorktimesnow.com)
Proteins have hydrophilic and hydrophobic regions depending on the side chains of the amino acids and the protein configuration, and these regions can promote or hinder the biotinylation process depending on the solubility of the biotinylation reagent. (newyorktimesnow.com)
Avidin2
Now you can measure the degree of biotinylation in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. (vectorlabs.com)
The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance for binding to avidin molecules. (thermofisher.com)
Antibodies1
EXPERIMENTAL PROCEDURES Antibodies and Reagents Antibodies against mouse caspase-1 Asc Nlrp3 and Nlrc4 have been described previously (16 17 Murine IL-1β antibody (AF-401-NA) was purchased from R&D Systems. (sciencepop.org)
Proteins8
Enzymatic biotinylation can be carried out in vitro but BirA also reacts specifically with its target peptide inside mammalian and bacterial cells and at the cell surface, while other cellular proteins are not modified. (wikipedia.org)
Specific labeling of cell surface proteins is another useful application for these water-soluble and membrane-impermeable reagents. (thermofisher.com)
Cell surface biotinylation and immunofluorescence analyses with confocal microscopy indicated that rMATE1 mutant proteins were localized at plasma membranes. (aspetjournals.org)
Therefore, biotinylation is widely used in biomedical fields, and biotinylated proteins are widely used in biotechnology as a molecular tool. (newyorktimesnow.com)
Western blotting for any protein expressed exclusively in an intracellular compartment which include the endoplasmic reticulum may be used to detect biotinylation of intracellular proteins. (adenosine-receptor.com)
Nonetheless, in our knowledge, the ratio of biotinylated/WCL ezrin or actin is 1:1,000 and as a result, these proteins are appropriate for determination of cell membrane integrity in the biotinylation-based assays. (adenosine-receptor.com)
We have discussed proteins in our blog ' Know your protein ' so naturally have decided to follow up with a discussion on sensor chips and reagents. (antibodyanalytics.com)
Thermo Scientific Pierce HABA is 4′-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules. (resurrectionofgavinstonemovie.com)
Reactive2
sCIP is compatible with both MS1- and MS2-based quantitation, and the sCIP-MS2 method is distinguished by its click-assembled isobaric tags in which the reporter group is encoded in the sCIP capture reagent and balancer in the pan cysteine-reactive probe. (bvsalud.org)
Widely adopted chemoproteomic sample preparation workflows rely on the use of pan cysteine-reactive probes such as iodoacetamide alkyne combined with biotinylation via copper-catalyzed azide-alkyne cycloaddition (CuAAC) or "click chemistry" for cysteine capture. (bvsalud.org)
Oligos1
With our extensive library of reagents, you can synthesise oligos with your modification of choice-from conjugation chemistry to cell delivery to probe detection, we've got you covered. (biosearchtech.com)
Conjugation1
Chemical biotinylation utilises various conjugation chemistries to yield nonspecific biotinylation of amines, carboxylates, sulfhydryls and carbohydrates (e.g. (wikipedia.org)
Streptavidin1
This biotinylation reaction can also go to completion, meaning that the product is generated with high uniformity and can be linked to streptavidin in a defined orientation e.g. for MHC multimers. (wikipedia.org)
Azide-alkyne cycloaddition1
This product enables biotinylation of molecules using either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC), ruthenium-catalyzed azide-alkyne cycloaddition (RuAAC), or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. (quantabiodesign.com)
Nucleic1
We offer a diverse array of biotinylation reagents for labelling nucleic acids. (biosearchtech.com)
Our wide range of reagents with unrivaled quality provides a single source for your oligonucleotide synthesis reagents-simplifying your supply chain so you can focus on your science and accelerate your path forward. (biosearchtech.com)
To address limitations of established chemoproteomics workflows, including cumbersome reagent synthesis and low throughput sample preparation, here, we established the silane-based cleavable isotopically labeled proteomics (sCIP) method. (bvsalud.org)
Histidine2
The transport of TEA via rMATE1 was inhibited by the sulfhydryl reagent p -chloromercuribenzenesulfonate (PCMBS) and the histidine residue modifier diethyl pyrocarbonate (DEPC) in a concentration-dependent manner. (aspetjournals.org)
Properties of the ligand of interest, e.g. histidine tag, biotinylation, Fc tail or no specific tag at all, dictate the choices of chip available to you. (antibodyanalytics.com)
Enables1
The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability. (thermofisher.com)
Dyes1
From gold-standard quenching reagents to our patented dyes, find the phosphoramidite, solid support and reagents you need to generate the ideal fluorescent signal. (biosearchtech.com)
Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. (thermofisher.com)
See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products. (quantabiodesign.com)
Functional groups1
Biotinylation reagents can be used to target a variety of functional groups, including primary amines, thiols, carbohydrates, and carboxyl groups. (newyorktimesnow.com)
Chemicals1
Mimotopes supplies and distributes peptide reagents and chemicals both within and outside of Australia. (nridigital.com)
Stability1
Increase oligo duplex stability with high-quality amidites and reagents for backbone modifications. (biosearchtech.com)
Efficiently1
In our expertise the nonreacted biotinylation reagent and GSH can be efficiently removed by gentle washing with PBS++ with out the use of quenching reagents. (adenosine-receptor.com)
Cell3
Because of the hydrophobicity of NHS-esters, NHS biotinylation reagents can also diffuse through the cell membrane, meaning that they will biotinylate both internal and external components of a cell. (wikipedia.org)
Her student Christopher Go created 200 stable cell lines that each express one organelle marker as a probe and performed proximity-dependent biotinylation to identify the composition of each organelle. (asbmb.org)
This functional group-specific variety is useful in selecting biotinylation reagents that do not inactivate the target macromolecule. (newyorktimesnow.com)
Extending the recently introduced labile mode of the MSFragger search engine, here we report an in-depth analysis of cysteine biotinylation via click chemistry (CBCC) reagent gas-phase fragmentation during MS/MS analysis. (bvsalud.org)
Wide range1
There is a large number of biotinylation reagents available that exploit the wide range of possible labelling methods. (wikipedia.org)
She is committed to sharing data, methods, reagents and protocols and to helping other scientists realize their objectives. (asbmb.org)
Loss1
The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. (thermofisher.com)
Technology1
This technology, in works with PEG as a blocking reagent, showed limited cross-reactivity when tested with Japanese Encephalitis Virus, which shares 56.5% sequence homology to ZIKV NS1. (broadpharm.com)