Biological k562 cells. Medical search. Frequent questions

After the treatment time period induced apoptosis in K562 cells without increasing G2/M-phase cells. (ewha.ac.kr)

Here we design a high-throughput reporter assay called enhancer × promoter self-transcribing active regulatory region sequencing (ExP STARR-seq) and applied it to examine the combinatorial compatibilities of 1,000 enhancer and 1,000 promoter sequences in human K562 cells. (nature.com)
Cell viability was measured using the MTT assay. (hindawi.com)
5(R),7(S)-bisepoxy-substituted compound 11 was the most potent DNA cross-linker and induced G2/M arrest in a cell cycle assay in a dose- and time-dependent manner. (ewha.ac.kr)
Because Kaiso is thought of a methylation dependent op portunistic oncogene, it had been conceivable to discover the biological purpose of Kaiso over the cells growth in vitro, the pro liferation of K562 cells was evaluated by a WST one assay. (srcsignaling.com)
Cytotoxicity of different concentrations of extracts (acetone 20, 50 and 100 %, ethanol 20, 50 and 100%, acetone dichloromethane (1-1), and pure methanol) was evaluated against cultured cells, using MTT assay.Findings: Acetone-dichloromrthane (1-1) then pure acetone extract were the most cytotoxic extracts. (ac.ir)
Here, we use a human cell-based reporter assay to characterize off- target cleavage of Cas9-based RGENs. (cdc.gov)

in other words, it differentiates the weak binding sites which are reproducible across replicated samples from background signals (i.e., artifactual binding sites), taking into consideration if the replicates are biological or technical [ 8 ]. (biomedcentral.com)
Chromatin Conformation Capture in two biological replicates of K562 cells to identify interactions between tRNA genes in human cells. (nih.gov)
Final results are introduced as an average of three organic replicates and info is revealed as imply six SEM relative to non-nucleofected cells. (jnkinhibitor.com)
Outcomes are introduced as an typical of 3 biological replicates and the relative endogenous E3: E4 ratio was established by normalizing to the endogenous E3: E4 ratio of K562 cells that have been not nucleofected with siRNA. (jnkinhibitor.com)

Raw and processed data for ExP STARR-seq, motif ExP STARR-seq, HS-STARR-seq and K562 PRO-seq can be found at the NCBI's Gene Expression Omnibus under accession number GSE184426 . (nature.com)
We analyzed differential gene expression in the peripheral white blood cells (WBC) from 7 female patients diagnosed with occupational benzene poisoning and 7 matched controls. (cdc.gov)
The reproducibility and low noise of this high-throughput single cell data allowed us to deconstruct cell populations and infer gene expression relationships. (uth.edu)
However, SALL4 expression status and related pathway in bone marrow (BM) cells and relationship with somatic gene mutations in MDS patients has not been explored. (confex.com)
MBS is required for induction of singlet oxygen-dependent gene expression and, upon oxidative stress, accumulates in distinct granules in the cytosol of Arabidopsis cells. (refine.bio)
Offered that Kaiso is overexpressed while in the cytoplasm of K562 cells, this study set out to examine how reduction of Kaiso and Regorafenib VEGFR their companion p120ctn impacted gene expression and cell proliferation of CML BP. (srcsignaling.com)
K562 cells were www.selleckchem.com/products/Bortezomib.html both transfected with siRNA scrambled that will not target any human gene or transfected with siRNA to Kaiso or p120ctn either alone or in combination. (srcsignaling.com)
Remarkably, our results show POLR2A and H3K36me3 can highly and consistently predict gene expression in three cell lines. (oncotarget.com)
Earlier studies [ 1 - 4 ] showed transcription factors (TFs) binding and histone modifications (HMs) were critical for gene expression, and the abnormities of TFs binding and HMs may affect the cell fate such as differentiation and apoptosis [ 5 ]. (oncotarget.com)
Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
details] The synergic effects of Crocus sativus L. and low frequency electromagnetic field on VEGFR2 gene expression in human breast cancer cells [med. (emf-portal.org)

In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)

Therefore, a central goal in clinical care of SDS patients is to identify incipient leukemic transformation and initiate pre-emptive treatment with allogeneic stem cell transplantation. (nature.com)
Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo, in human promyelocytic leukemic cell lines, and ex vivo in human blood neutrophils. (cdc.gov)
Studies in murine models, as well as in human samples, have demonstrated that SALL4 is essential for leukemic cell survival and that SALL4 transgenic mice develop an MDS-like phenotype prior to transformation to AML, suggesting that SALL4 can be a driver of MDS/AML pathogenesis. (confex.com)

Similar results were observed in Lin-CD34+CD38- hematopoietic stem cells, CD11b+ mature myeloid cells, CD19+ B cells, CD235+ erythroid cells. (confex.com)

Compared to healthy controls (n=24), DLBCL patients (n=33) showed significant lymphopenia, due to low CD3 + CD4 + T helper and CD3 - CD56 + NK cell counts, while cytotoxic CD3 + CD8 + T cell counts were similar. (frontiersin.org)
In vitro , CD3 + CD8 + CD27 - CD28 - compared to CD3 + CD8 + CD27 + CD28 + CART cells displayed similar CD19 + target cell-specific cytotoxicity, but were hypoproliferative and produced less cytotoxic cytokines (IFN-γ and TNF-α). (frontiersin.org)
In our earlier studies, some novel derivatives of etodolac exhibited stronger cytotoxic effects on prostate cell lines and had similar effects on leukemia cells in pre-screening experiments. (ksbu.edu.tr)

In the present study, we tested whether CX-5461 could potentiate the effect of imatinib in the human chronic myeloid leukemia cell line K562. (mendeley.com)
Cells were divided into 3 treatment groups (with 3 independent biological replications in each group): control, imatinib (100 nM for 48 hr) alone, and imatinib+CX-5461 (100 nM for 48 hr). (mendeley.com)
To verify the cytoplasmic localization of Kaiso in CML BP, we analyzed cytoplasmic Inhibitors,Modulators,Libraries expression of Kaiso protein by western blot examination, evaluating expression in cytoplasmic and nuclear protein extracts in K562 cell line and imatinib resistant K562 cell line. (srcsignaling.com)
Important cytoplasmic expression of Kaiso was only observed in K562 cell line whereas in imatinib resistant K562 cell line was clearly down regulated. (srcsignaling.com)
We also confirmed the weak expression of Kaiso in imatinib resistant K562 cell line by immunofluorescence. (srcsignaling.com)
As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild‑type) or TCCY‑T315I (human imatinib‑resistant) and the Ba/F3‑(T315I/E279K/Y253H) (mouse BCR/ABL point mutation‑transfected cells). (spandidos-publications.com)
Interestingly, AVM appeared to be more sensitive to imatinib‑resistant (T315I, Y253H, and E279K) than wild‑type BCR/ABL cells, indicating its potential to overcome imatinib‑resistant severe issues in CML. (spandidos-publications.com)
Thus, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 [human wild-type (WT)] or TCCY-T315I [human imatinib-resistant (IR)] and the Ba/F3-(T315I/E279K/Y253H) (mouse BCR/ABL point mutation-tranfected cells). (spandidos-publications.com)
Consistent with the results obtained using imatinib, CFTRinh-172 led to a significant reduction in p-BCR-ABL in both K562 and SUP-B15 cells as well as significant down-regulation oft-BCR-ABL in K562 cells (Figure 3A) but not in SUP-B15 cells (Figure 3C). (abmole.com)

Compound 11 showed the most potent cell antiproliferative activity against all tested cancer cell lines with particularly strong inhibition (an IC 50 of 0.04 µM) of K562 myelogenous leukemia cancer cell proliferation. (ewha.ac.kr)
Series of heterocyclic compounds containing 3-spiro[3-azabicyclo[3.1.0]hexane]oxindole framework have been studied for their antiproliferative activity against K562, Jurkat, HeLa, CT26 cell lines. (sciforum.net)

These defects are accompanied with a reduced survival of Sox6-/- red blood cells, resulting in a compensated anemia. (edu.sa)
Adenine improves the survival of rat Purkinje cells and enhances the storage of erythrocyte in whole blood [ 6 - 8 ]. (hindawi.com)
The aim of this study is to evaluate the survival benefit of consolidative autologous hematopoietic stem cell transplantation (ASCT) in patients with peripheral T-cell lymphomas (PTCL). (bvsalud.org)
RNAi knock down of kaiso in K562 cells improves survival and proliferation. (srcsignaling.com)
MiRNA-486 regulates angiogenic activity and survival of mesenchymal stem cells under hypoxia through modulating Akt signal[J]. Biochem Biophys Res Commun, 2016, 470:670-677. (magtechjournal.com)
Cancer is the uncontrolled growth of cells in the body, which is caused by the stepwise accumulation of mutations that affect cell growth control, differentiation and survival. (nutraculture.com)
Myeloid leukemia cells maintain a high intracellular ROS level and use redox signals for survival. (ncku.edu.tw)
Static or 50 Hz magnetic fields at 100 µT do not modify the clonogenic survival of doxorubicin-treated MCF-7 cancer cells [med. (emf-portal.org)

مطالعه‌ی حاضر با هدف بررسی هم‌خوانی نتایج حاصله از معرفی یک سیستم بهینه‌ی حلال جهت استخراج تاکسان‌ها از گونه‌ی Taxus baccata با مطالعات بیولوژیک انجام گرفت.روش‌‌ها: سه رده‌ی سلولی MDA-MB-468، Hela و K562 در محیط RPMI-1640 با 10 درصد سرم جنین گاوی کشت داده شدند. (ac.ir)
This study was performed to confirm the consistency of results obtained by extraction of Taxanes by optimal solvent system using biological assessment.Methods: Three cell lines including MDA-MB-468, Hela and K562 were cultured in PRMI-1640 medium with 10% fetal bovine serum. (ac.ir)

However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)
In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)
Overall, compound 11 showed good consistent inhibitory biological activity related to cancer cell proliferation. (ewha.ac.kr)
Even though the Kaiso knock down alone didn't show a substantial enhance proliferation, the double knock down showed a significant boost by 51% in proliferation, when compared to scrambled knock down cells. (srcsignaling.com)
Nonetheless, knock down of p120ctn alone won't impact proliferation, when when compared to scrambled knock down cells. (srcsignaling.com)
This considerable boost in SCF expression correlated with a rise on in vitro cell proliferation. (srcsignaling.com)
In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
Growth of cancer cells is regulated by the balance between cell proliferation and apoptosis, which is a normal physiological process serving to eliminate unwanted cells and maintain homeostasis in healthy tissue. (nutraculture.com)
The role of SOD1 is verified when ATN-224, a SOD1 specific inhibitor, inhibits cell proliferation and promotes cell death in myeloid leukemia cells without PMA treatment. (ncku.edu.tw)
Finally, ATN-224 enhanced the inhibition of cell proliferation by Ara-C. Taken together, the results demonstrate that SOD1 regulates cell death and differentiation in myeloid leukemia cells. (ncku.edu.tw)

SP1 and GATA3) and HDAC2-GATA1 regulatory networks on rescued regions in K562 cell line. (biomedcentral.com)
We observed a statistically significant correlation between b-catenin and WT1 expression across 16 myeloid cell lines, with 50% (8/16) co-expressing b-catenin and WT1 to varying degrees ( Figure 1A , B). There was no particular association of this correlation with cell line morphology or genotype. (haematologica.org)
Chronic myeloid leukemia is a common cancer in human, so the goal of this study was the use of natural compound such as cell wall obtained from Saccharomyces cerevisiae (S. cerevisiae) and Saccharomyces boulardi (S. boulardi) and zinc nanoparticles on the growth inhibition of K562 cell line. (ac.ir)
The results showed that S. boulardi cell wall significantly (p=0.029) inhibits the growth of K562 cell line compared to S. cerevisiae . (ac.ir)
Also zinc nanoparticles significantly (p=0.021) inhibit K562 cell line. (ac.ir)
Based on this finding it should be concluded that combination of zinc nanoparticles with Saccharomyces cell walls could inhibit the growth of K562 cell line in vitro . (ac.ir)
Induction of CYP4F3 by phenol was also observed in differentiated HL-60 cells, in pro-erythroid cell line K562, and ex vivo in human neutrophiles. (cdc.gov)
Nevertheless, the p120ctn knock down alone showed a reduce by 65% in B catenin ranges whilst the Kaiso p120ctn double knock down line didn't substantially affect B catenin levels in vitro when compared to scrambled knock down cells. (srcsignaling.com)
Objective: Using the K562 leukemia cell line as a model, we sought to investigate the anti-cancer properties of a hydrazide-hydrazone derivative (SGK-205) and a 4-thiazolidinone derivative of etodolac (SGK- 216). (ksbu.edu.tr)

Anti - cancer property of different concentrations of the yeasts' cell wall with zinc nanoparticles were assayed by MTT and electrophoresis methods. (ac.ir)
Materials and Methods: In the current investigation, SGK-205 and SGK-216 compounds were administered to K562 cells for 24 and 48 hours at concentrations of 10, 25, 50, 75 and 100 μM. (ksbu.edu.tr)

Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
1 Wnt/b-catenin is also known to drive the emergence and maintenance of leukemia stem cells in AML. (haematologica.org)
A) Immunoblot of myeloid leukemia cell lines showing the relative level of b-catenin (~92 kDa) and WT1 (~50 kDa) protein, with b-actin (42 kDa) used to assess protein loading. (haematologica.org)
Exposure to benzene elicits a spectrum of hematotoxicity ranging from reduction of peripheral blood cell counts to aplastic anemia and leukemia. (cdc.gov)
Mechanistic analysis revealed that phenol , a major metabolite of benzene, significantly induced the expression of CYP4F3A at both mRNA and protein levels in cultured promyelocytic leukemia cells (HL-60) similarly to all-trans retinoic acid (ATRA). (cdc.gov)
Exosomal miR-486 regulates hypoxia-induced erythroid differentiation of erythro-leukemia cells through targeting Sirt1[J]. Exp Cell Res, 2017, 351:74-81. (magtechjournal.com)
Superoxide dismutases (SODs) are major antioxidant enzymes that have high levels of expression in myeloid leukemia cells. (ncku.edu.tw)
However, the role of SODs in the regulation of myeloid leukemia cells' biological function is still unclear. (ncku.edu.tw)
To investigate the function of SODs in myeloid leukemia cell death and differentiation, we used myeloid leukemia cell lines K562, MEG-01, TF-1, and HEL cells for this study. (ncku.edu.tw)
We found that PMA-induced megakaryocytic differentiation in myeloid leukemia cells is accompanied by cell death and SOD1 down-regulation, while SOD2 expression is not affected. (ncku.edu.tw)
Thus, SOD1 expression is required for myeloid leukemia cell fate determination. (ncku.edu.tw)

Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. (novusbio.com)

The findings demonstrated, for the first time, that benzene and metabolites induce CYP4F3 in human blood cells both in vivo and in vitro. (cdc.gov)
The effect of pulse repetition frequency on the uptake into electropermeabilized cells in vitro with possible applications in electrochemotherapy [med. (emf-portal.org)

Sox6-overexpression in K562 cells and in human primary ex vivo erythroid cultures enhances erythroid differentiation and leads to hemoglobinization, the hallmark of erythroid maturation. (edu.sa)
To obtain an overview on processes downstream to Sox6 expression, we performed a differential proteomic analysis on human erythroid K562 cells overexpressing Sox6. (edu.sa)
And H3K4me3, H3K27me3 and H3K9ac are more reliable predictors than other histone modifications in human embryonic stem cells. (oncotarget.com)
Synergy of micro and nanosecond electrical pulses with chemotherapeutics on human cancer cell viability [med. (emf-portal.org)
We readily detected off-target alterations induced by four out of six RGENs targeted to endogenous loci in human cells by examination of partially mismatched sites. (cdc.gov)
Our work demonstrates that RGENs are highly active even with imperfectly matched RNA-DNA interfaces in human cells, a finding that might confound their use in research and therapeutic applications. (cdc.gov)
Recent work has demonstrated that clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems1-3 can serve as the basis of a simple and highly efficient method for performing genome editing in bacteria, yeast and human cells, as well as in vivo in whole organisms such as fruit flies, zebrafish and mice4-13. (cdc.gov)
Although Maraffini and colleagues7 recently performed a systematic investigation of Cas9 RGEN specificity in bacteria, the specificities of RGENs in human cells have not been extensively defined and, to our knowledge, bona fide off-target mutations induced by Cas9 have not been identified in any eukaryotic cell or organism. (cdc.gov)
Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)
To begin to define the specificity determinants of RGENs in human cells, we sought to perform a large-scale test in which we assessed the effects of systematically mismatching various positions within multiple gRNA/target DNA interfaces. (cdc.gov)

K562 cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and antibiotics. (mendeley.com)
We have compared the enhancer activity of this core element with a synthetic enhancer lacking the factor binding sites flanking the AP1/NF-E2 motif (HS2(M)). In fetal/erythroid K562 cells, enhancement of a linked γ-promoter was significantly greater with wild-type HS2 than with HS2(M). In contrast, the increase in β-promoter activity in these cells was equivalent with either enhancer fragment. (monash.edu)

Apoptosis has proven to be tightly interwoven with other essential cell pathways. (wikipedia.org)
Although a large burst of reactive oxygen species (ROS) is known to lead to cell damage, a moderate release of ROS from the mitochondria, which occurs during nonlethal short episodes of ischemia, can play a significant triggering role in the signal transduction pathways of ischemic preconditioning leading to reduction of cell damage. (wikipedia.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
In particular, the invention relates to microfluidic devices and their uses and methods for assaying cells. (justia.com)
Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
Recent advances in synthesis of complex heterocyclic systems have led to significant increase in interest in development of efficient methods for synthesis of thereof as potential drugs or biological probes. (sciforum.net)
U2OS.EGFP cells caused by inactivating frameshift insertion/deletion (indel) mutations introduced by error prone non-homologous end-joining (NHEJ) repair of nuclease-induced double-stranded breaks (DSBs) (Fig. 1a and Methods ). (cdc.gov)

Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. (novusbio.com)
This outcome was confirmed by immunofluorescence in K562 cells transfected by siRNA Kaiso, exhibiting the undetectable ex pression of Kaiso. (srcsignaling.com)

Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
Adenine forms several biological compounds involved in a variety of cellular physiological processes such as adenosine triphosphate (ATP) in cellular respiration and deoxyribonucleic acid (DNA) in protein synthesis. (hindawi.com)
Results: In K562 cells, there was a definite dose-dependent response to SGK-205 and SGK-216 compounds. (ksbu.edu.tr)
In agreement with DNA cytometry studies, the tested compounds have achieved significant cell-cycle perturbation with higher accumulation of cells in G0/G1 phase. (sciforum.net)
Also we discovered that number of cells with filopodium-like membrane protrusions was significantly reduced after treatment with some of tested compounds (from 92 % in control cells up to 36% after treatment). (sciforum.net)

The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. (justia.com)
Biased or noisy datasets (with a high number of false negative or false positive peaks) negatively impact downstream biological and computational analyses [ 8 ]. (biomedcentral.com)

Using confocal microscopy, we found that actin filaments disappeared and granular actin was distributed diffusely in the cytoplasm in up to 40% of treated cells. (sciforum.net)

Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
In addition, RT-qPCR was used for the miR-486, Sirt1 or expression in K562 cells after the IH treatment, miR-486 over-expression or miR-486 inhibition. (magtechjournal.com)
Moreover, inhibition or silencing of SODs further increases cell death and decreases polyploidization induced by PMA while they were partially reversed by SOD1 overexpression. (ncku.edu.tw)

Conclusions miR-486 regulates the biological process of OSAHS patients with secondary polycythemia by targeting at Sirt1 . (magtechjournal.com)

These genes play a significant role in biological processes, not only in MDS but also in the occurrence and development of other diseases. (bvsalud.org)
Forkhead box protein M1 (FOXM1) is a key transcription factor (TF) that regulates a common set of genes related to the cell cycle in various cell types. (mdpi.com)
In further study, we find that even though transcription factors and histone modifications offer similar effects on expression levels of genome-wide genes, the effects of transcription factors and histone modifications on predictive abilities are different for genes in independent biological processes. (oncotarget.com)

It is of interest to explore the inhibitory effect of adenine on the growth of colorectal cancer cells and to determine the underlying mechanism. (hindawi.com)

Our findings suggest that adenine inhibits the growth of colon cancer cells. (hindawi.com)

Adenine has been suggested to induce cell cycle arrest in cancer cells, leading to cell death [ 9 , 10 ]. (hindawi.com)

Chimeric antigen receptor T (CART) cell therapy targeting the B cell specific differentiation antigen CD19 has shown clinical efficacy in a subset of relapsed/refractory (r/r) diffuse large B cell lymphoma (DLBCL) patients. (frontiersin.org)
Using this technique, we analyzed mouse embryonic stem cells, revealing in detail the population structure and the heterogeneous onset of differentiation after LIF withdrawal. (uth.edu)
RNAi knock down of kaiso in K562 cells block hematopoietic differentiation. (srcsignaling.com)
The metabolism of ROS also affects cell fate, including cell death and differentiation. (ncku.edu.tw)

Some cells had been also nucleofected with siRNA duplexes concentrating on both hnRNP H and F (siF1 and siH1, siF2 and siH2). (jnkinhibitor.com)

2 Protein interactions are critical to the stability, localization and activity of b-catenin, and we recently performed the first proteomic analyses of the b-catenin interactome in myeloid cells. (haematologica.org)
3 This study identified Wilms tumor protein (WT1) as a putative novel interaction partner in myeloid cells. (haematologica.org)
In order to idenitfy appropriate cell lines in which to study b-catenin:WT1 interplay we first performed a screen of myeloid cell lines to examine b-catenin and WT1 protein expression. (haematologica.org)
WT1 is an RNA-binding protein, 6 and β-catenin has also been shown to bind RNA, 7 so to confirm this interaction was not indirect via RNA binding we repeated WT1 Co-IP (with or without CHIR99021) after first confirming complete digestion of RNA through RNase A pre-treatment of cell lysates ( Figure 1E ). (haematologica.org)
The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)
shMTHFR enhanced cytosolic and nuclear SHMT/DHFR/TYMS 1 protein expression 2 in HepG2 cells. (bioz.com)
Lower MTHFR is associated with increased proportional cell populations in the G2/M phase, increased cytosol, and nuclear SHMT1/DHFR/TYMS protein expression under folate deficiency. (bioz.com)
QRT PCR evaluation showed that Kaiso mRNA levels had been decreased by 80% and Western blot examination showed that Kaiso protein ranges have been undetectable in K562 cells trans fected by siRNA Kaiso, when when compared to scrambled knock down cells. (srcsignaling.com)
The main goal of a ChIP-seq experiment is the detection of protein-DNA binding sites and histone modifications genome-wide in various cell lines and tissues. (biomedcentral.com)
It is established that Phycocyanin induces apoptosis in cancer cells by changing the Bcl-2/Bax ratio (Bcl-2 is an anti apoptotic protein, Bax is a pro-apoptotic protein, the ratio of Bcl-2/Bax represents the degree of apoptosis) and the release of Cyt-c in the cytosol (Lu et. (nutraculture.com)
Similarly, insertion of the stage selector protein site into the β-promoter boosted enhancer activity observed with HS2 but not HS2(M). In adult erythroid MEL cells, enhancement of a linked β-promoter was significantly greater with HS2 than with HS2(M). This effect was dependent on the binding of the adult stage-specific factor, erythroid Kruppel-like factor, to the β-promoter. (monash.edu)

We further validated the interaction in two other myeloid cell lines (KG-1 and K562) ( Online Supplementary Figure S1A ) and confirmed that this interaction also occurs in the nucleus ( Figure 1D ). (haematologica.org)
Association between b-catenin and WT1 in myeloid cell lines. (haematologica.org)

Despite this heterogeneous response, blood pre-infusion biomarkers predicting responsiveness to CART cell therapy are currently understudied. (frontiersin.org)

D) Ratio of endogenous BIM transcripts harboring exon 3 more than exon 4 after nucleofecting K562 cells with the indicated siRNAs. (jnkinhibitor.com)
B) Genuine-time RT-PCR investigation of RNA from K562 cells nucleofected with either control or hnRNP C-distinct siRNA duplexes to evaluate the ratio of endogenous exon three- to exon four-made up of BIM transcripts. (jnkinhibitor.com)

Pulsed electromagnetic field potentiates etoposide-induced MCF-7 cell death [med. (emf-portal.org)

Cell viability was assessed using the MTT test, and apoptosis by Annexin V-PI staining and mitochondrial membrane potential assays, together with mRNA expressions of apoptotic proteins. (ksbu.edu.tr)

However, the vast majority of biological knowledge has emerged as a consequence of studying cell populations and not individual cells. (justia.com)

However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)

We evaluated the expression of SALL4 using single-cell mass cytometry (CyTOF) utilizing 28 antibodies including surface lineage markers and intracellular proteins, such as p53, ki67, c-myc and pAKT to identify SALL4 expressed cells and related pathway in bone marrow (BM) for 10 MDS patients. (confex.com)

For cells, cell lines and tissues in culture till half confluency. (hoxa2.com)

Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
In this study, we evaluated the expression of SALL4 and related factors using single-cell mass cytometry (CyTOF) and NanoString technology in MDS patients alongside with target sequence for MDS-related mutations. (confex.com)
We observed that Lin-CD34+CD38+ progenitor cells in 8 out of the 10 MDS patients had higher SALL4 expression levels, while the progenitor cells of normal BM cells did not express SALL4. (confex.com)
Conversely only 3 MDS patient had weak SALL4 expression in CD3+ T cells. (confex.com)
shMTHFR promoted nuclear MLH1/p53 expression under folate deficiency that can protect cells from folate depletion induced micronuclei and uracil misincorporation. (bioz.com)
48 hours later, true-time RT-PCR evaluation of RNA from K562 cells was carried out to decide the relative expression of (A) hnRNP H and (B) hnRNP F transcripts. (jnkinhibitor.com)
Consistent with this particular discovering, knock down of both Kaiso or p120ctn alone or in combin ation, in K562 cells, led to a significant 10 100 fold in crease in SCF expression assessed by QRT PCR. (srcsignaling.com)
Peripheral blood mononuclear cell (PBMC) miR-486, Sirt1 and GATA-1 expression were measured by RT-qPCR, and the concen- tration of plasma Sirt1 was measured by ELISA. (magtechjournal.com)
GATA-1 mRNA expression was checked by RT-qPCR in Sirt1 inhibited K562 cells. (magtechjournal.com)
4)IH promoted the expression of miR-486( P ＜0.01) and inhibited Sirt1 expression in K562 cells( P ＜0.01). (magtechjournal.com)
No effects of pulsed electromagnetic fields on expression of cell adhesion molecules (integrin, CD44) and matrix metalloproteinase-2/9 in osteosarcoma cell lines [med. (emf-portal.org)

Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
Extremely low-frequency magnetic fields significantly enhance the cytotoxicity of methotrexate and can reduce migration of cancer cell lines via transiently induced plasma membrane damage [med. (emf-portal.org)

Journal of Biological Chemistry , 273 (22), 13593-13598. (monash.edu)

The following research aimed to determine a possible complex formation between bone biological substrates, such as proteins and oxytetracycline (OTC), an antibiotic approved for use in animal husbandry, capable of determining a toxic effect on K562 cells. (forza10usa.com)

In later stages of apoptosis the entire cell becomes fragmented, forming a number of plasma membrane-bounded apoptotic bodies which contain nuclear and or cytoplasmic elements. (wikipedia.org)
In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells exhibited less apoptotic responses. (hindawi.com)
Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway. (hindawi.com)
Pulsed electromagnetic field with temozolomide can elicit an epigenetic pro-apoptotic effect on glioblastoma T98G cells [med. (emf-portal.org)

SALL4 is a transcription factor that is important for development and embryonic stem cell properties. (confex.com)

Cell viability and apoptosis rate of HSPC-1 were detected by MTT and flow cytometry, respectively. (bvsalud.org)

On the other hand, hydroquinone induced extensive apoptosis of the cells. (cdc.gov)
OBJECTIVE: To investigate the regulatory effect and mechanism of DNA methyltransferase 3A (DNMT3a) in hydroquinone-induced hematopoietic stem cell toxicity. (bvsalud.org)

But these anti-cancer properties would warrant further study on the clinical application of yeast cell wall. (ac.ir)
In the present study, we investigated the anticancer effects of adenine and the underlying mechanism in colon cancer cells. (hindawi.com)
Recently, adenine has been explored for its anticancer property in several types of cancer cell lines. (hindawi.com)
We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)
Nutraculture™ Phycocyanin™ induces apoptosis in the existing and proliferating cancer cells and being a natural antioxidant, it also helps getting rid of any cancer promoting oxidative stress. (nutraculture.com)
Phycocyanin lowers the amount of cyclooxygenase-2 which is up regulated in cancer cells. (nutraculture.com)

Utilizing siRNA p120ctn a reduction of 70% in p120ctn was achieved when compared to scrambled knockdown cells by QRT PCR analysis. (srcsignaling.com)
In addition, the knockdown of PKD2 reduces cell death and promotes polyploidization induced by PMA. (ncku.edu.tw)

Right after one more 24 hours, genuine-time RT-PCR was executed on RNA from these cells to decide the ratio of exon 3- to exon four-containing minigene products. (jnkinhibitor.com)
minigene E3: E4 ratio was identified by normalizing to the E3: E4 ratio of K562 cells nucleofected with the identical minigene, but without having any siRNA. (jnkinhibitor.com)

It plays an important role during embryonal development as programmed cell death and accompanies a variety of normal involutional processes in which it serves as a mechanism to remove "unwanted" cells. (wikipedia.org)
Adenine is involved in a variety of cell biological processes and has been explored for pharmacological uses. (hindawi.com)

Anatomy15

Organisms15

Diseases5

Chemicals and Drugs58

Analytical, Diagnostic and Therapeutic Techniques and Equipment48

Psychiatry and Psychology1

Phenomena and Processes81

Disciplines and Occupations17

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture9

Information Science20

Health Care11

Apoptosis in K562 cells1

Assay6

Replicates4

Gene13

Chronic myelogen1

Leukemic3

Erythroid cells1

Cytotoxic3

Imatinib9

Antiproliferative activity2

Survival8

Hela2

Proliferation10

Line9

Concentrations2

Leukemia12

Jurkat1

Vitro2

Human10

Fetal2

Pathways3

Methods6

Immunofluorescence2

Compounds6

Downstream2

Cytoplasm1

Inhibition3

Conclusions1

Genes3

Inhibitory1

Inhibits1

Induce1

Differentiation4

Nucleofected with siRNA1

Protein11

Myeloid2

Heterogeneous1

Endogenous2

Potentiates1

Viability was assessed1

Populations1

Overexpression1

Intracellular1

Lines and tissues1

Expression11

Significantly2

Journal of Biolog1

Proteins1

Apoptotic5

Embryonic1

Flow cytometry1

Hydroquinone induced2

Cancer6

Knockdown2

Ratio2

Processes2