Biological escherichia coli proteins. Medical search. Frequent questions

Recent advances in genetic engineering have made it possible for microorganisms to be used for expression of heterologous proteins. (biomedcentral.com)
In this study, we expressed and characterized a new serine protease inhibitory protein (PI-QT) from the metagenome of sponge-associated microorganisms in Escherichia coli . (bmrat.org)
Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)

The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
The conversion of fibrinogen to fibrin at the surface of curliated E. coli bacteria leads to the generation of proinflammatory fibrinopeptides. (lu.se)
Here we show that Escherichia coli bacteria expressing fibrous surface proteins, known as curli, assemble and activate factors of the human coagulation cascade at their surface. (lu.se)
This system produces oscillations of two protein types, MinD and MinE, between two poles of the rod-shaped bacteria, positioning the machinery for cell division to midcell. (phys.org)
Produced by the National Institute of Allergy and Infectious Diseases (NIAID), this digitally-colorized scanning electron micrograph (SEM) depicts numbers of Escherichia coli , rod shaped bacteria, which were grown in a culture, and shown here attached to a coverslip. (cdc.gov)
Pathogens, such as bacteria, viruses and parasites, that cause disease in humans and animals may depend partially or entirely for their existence on other physical, chemical, or biological factors. (who.int)

A subset of periplasmic proteins are exported via the Tat pathway to which proteins are directed by N-terminal "transfer peptides" bearing the consensus (S/T)RRXFLK "twin-arginine" motif. (ox.ac.uk)

This distribution comprises a small fraction of protein-specific hydration sites, where water rotation is strongly retarded, and a dominant fraction of generic hydration sites, where water rotation is as fast as in the hydration shells of small peptides. (lu.se)

In this system, large combinatorial libraries of macrocyclic molecules are biosynthesized in Escherichia coli cells and simultaneously screened for their ability to rescue pathogenic protein misfolding and aggregation using a flow cytometric assay. (nature.com)
Deformylases were isolated from Escherichia-coli in an attempt to develop a competitive binding assay for detecting fMLP. (cdc.gov)

A genomically recoded Escherichia coli strain that lacks all amber codons and release factor 1 (C321. (northwestern.edu)
A has opened new opportunities in chemical and synthetic biology, this strain has not been optimized for protein production, limiting its utility in widespread industrial and academic applications. (northwestern.edu)
Total DNA from E. coli strain B purchased from Sigma-Aldrich (Cat# D4889) was used as standard. (scirp.org)
2.2 Protein expression and purification Escherichia coli strain HMS174 (DE3) cells (Novagen) were transformed with pGEX-hCK2?2. (immune-source.com)
Using an E. coli strain deficient with ALKB, we found that ALKBH6 complements ALKB deficiency and increases resistance after alkylating agent treatment. (frontiersin.org)
Here, we report the design of a pH, temperature, and protease-stable bivalent VHH-based protein BL1.2 that cross-links a F4 + ETEC model strain by selectively binding to its fimbriae. (dtu.dk)
Consistent with the results that activation of hTLR4/MD-2 signalling by the mutant cells was of several orders of magnitude lower compared with cells of the E. coli wild-type strain, heterologous proteins prepared from different mutant strains were free of endotoxic activity. (dnsalias.com)
The NMB0736 gene of Neisseria meningitidis serogroup B strain MC58 encodes the putative nitrogen regulatory protein, IIANtr (abbreviated to NM-IIANtr). (rcsb.org)

My research team has discovered several mechanisms by which bacterial proteins subvert the host innate immune system to promote bacterial colonization and transmission. (k-state.edu)

In this approach, heterologous proteins are expressed either as cytoplasmic or intracellularly or secreted as extracellularly [ 11 ]. (biomedcentral.com)

Protein stabilization upon ligand binding has frequently been used to identify ligands for soluble proteins. (nih.gov)

This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. (nih.gov)
This product is an active protein and may elicit a biological response in vivo, handle with caution. (abcam.com)
Large-scale experimental studies show unexpected amino acid effects on protein expression and solubility in vivo in E. coli. (rostlab.org)
However, it is anticipated that its biological activity in vivo will be higher than human Prolactin due to prolonged persistence in circulation. (prospecbio.com)
This protein inhibits F4 + ETEC adhesion to porcine epithelial cells ex vivo and decreases F4 + ETEC proliferation when administrated as a feed additive to weaned F4 + ETEC challenged piglets. (dtu.dk)

While one substrate of particular biological importance is often used to name the transporter or family, there may also be co-transported or leaked ions or molecules. (wikipedia.org)
Proteins, DNA and other large biological molecules are broken by water, oxygen, other chemicals and radiation. (signsofthetimes.org.au)
Of course, theory isn't the same as observation and proteins, along with other biological molecules, have now been observed in a lot of fossils. (signsofthetimes.org.au)

We study several virotypes of Escherichia coli that cause diarrhea and malnutrition in humans and livestock, including E. coli O157:H7, non-O157 STEC, and enterotoxigenic E. coli (ETEC). (k-state.edu)
A major challenge in industrial pig production is the prevalence of post-weaning diarrhea (PWD) in piglets, often caused by enterotoxigenic Escherichia coli (ETEC). (dtu.dk)

Disruption of plant plasma membrane by Nep1-like proteins in pathogen/plant interactions. (ki.si)

The two halves of the protein pack against each other in a clam-shell fashion, sealing via interactions at the ends of the transmembrane helices and extracellular loops. (wikipedia.org)
Nickel ABC transporter, substrate-binding protein NikA / Solute-binding protein family 5, conserved site / Bacterial extracellular solute-binding proteins, family 5 signature. (pdbj.org)
The production of recombinant mature hormone in suitable hosts and secretion of this therapeutic protein into the extracellular space can be considered as one of the best cost-effective approaches not only to obtain the active form of the protein but also endotoxin-free preparation. (biomedcentral.com)
Since the natural growth hormone signal peptide is of eukaryotic origin and is not detectable by any of the Escherichia coli secretory systems, including Sec and Tat, and is therefore unable to secrete hGH in the prokaryotic systems, designing a new and efficient signal peptide is essential to direct hGh to the extracellular space. (biomedcentral.com)
Since the native signal protein peptide of S. aureus protein A was not able to deliver hGH to the extracellular space, it was modified using bioinformatics tools and fused to the n-terminal region of hGh to show that the redesigned signal peptide was functional. (biomedcentral.com)

Heat-labile enterotoxin promotes Escherichia coli adherence to intestinal epithelial cells. (k-state.edu)

Research interests include determining protein structures and studying the structure/function of complexes formed with drugs and natural ligands. (nottingham.ac.uk)
Methods such as differential scanning fluorimetry (DSF) and differential static light scattering (DSLS) have been employed in the 384-well format and have been useful in identifying ligands that promote crystallization and 3D structure determination of proteins. (nih.gov)
FMPs were naturally occurring bacterial ligands that can cause biological effects at nanomolar concentrations. (cdc.gov)

We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. (nih.gov)
RNA codons and protein synthesis. (crossref.org)

Outcome of a workshop on archiving structural models of biological macromolecules. (rostlab.org)

The authors envision that their library of organisms will provide the community with multiple options for increased expression of proteins with new and diverse chemistries. (northwestern.edu)

In this study, using a combination of the bioinformatics design and molecular genetics, the protein A signal peptide from Staphylococcus aureus was modified, redesigned and then fused to the mature hGH coding region. (biomedcentral.com)

The growth of six industrially relevant strains of Escherichia coli was characterized under eight stress conditionsrepresentative of fed-batch fermentation, and strains W and BL21(DE3) were selected as platforms for transposon (Tn) mutagenesis due to favorable resistance characteristics. (m2p-labs.com)
This new series of genetically engineered E. coli strains lacking LPS holds promise for the manufacture of endotoxin-free products. (dnsalias.com)

The structural biology laboratory utilises a variety of techniques including Protein Crystallography, Cryo-electron Microscopy and associated techniques such as Isothermal Titration Calorimetry, which are then combined with functional studies or serve as a basis for structure-based drug design. (nottingham.ac.uk)
Combining NMR and small angle X-ray and neutron scattering in the structural analysis of a ternary protein-RNA complex. (ibs.fr)
Structural basis of O6-alkylguanine recognition by a bacterial alkyltransferase-like DNA repair protein. (rostlab.org)
Solution NMR structure of the NlpC/P60 domain of lipoprotein Spr from Escherichia coli: structural evidence for a novel cysteine peptidase catalytic triad. (rostlab.org)
Solution NMR structure of Escherichia coli ytfP expands the structural coverage of the UPF0131 protein domain family. (rostlab.org)

Here we refashion Escherichia coli metabolism by expressing a phosphoribulokinase-neomycin phosphotransferase fusion protein to produce a high-fidelity, high-throughput Rubisco-directed evolution (RDE2) screen that negates false-positive selection. (illinois.edu)
Our approach is evaluated on time series expression data measured in response to physiological changes that affect tryptophan metabolism in E. coli. (duke.edu)

Complementation analysis indicates that while TatA and TatE are functionally interchangeable, the TatB protein is functionally distinct. (ox.ac.uk)
The sensitive cocklebur ALS cDNA, fused with a glutathione S -transferase, was functionally expressed in Escherichia coli . (weedscience.com)

Proteins kinase CK2 (previously called casein kinase 2) is a pivotal and ubiquitously expressed member of the protein CX-6258 manufacture kinase CMGC subfamily which includes cyclin-dependent kinases (CDKs) mitogen-activated protein kinases (MAPKs) glycogen synthase kinases (GSKs) and CDK-like kinases (CLKs). (immune-source.com)
and it has been within many diseases especially cancer rendering it an interesting focus on inside CX-6258 manufacture the druggable category of eukaryotic proteins kinases (Pagano et al. (immune-source.com)

For example, it can have inhibitory effects on glycolysis followed by a direct effect on protein synthesis [ 2 ], as well as increase the absorption and retention of calcium, magnesium and phosphate ions in the body. (biomedcentral.com)

Protein abundance profiling of the escherichia coli cytosol. (igem.org)

A) enables efficient genetic encoding of chemically diverse non-canonical amino acids (ncAAs) into proteins. (northwestern.edu)
Prolactin Human Recombinant Pegylated produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids + an additional Ala at n-terminal. (prospecbio.com)
Pegylated Leptin N82K Human Recombinant produced in E.Coli is a single non-glycosilated polypeptide chain containing 146 amino acids, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus acids and having a molecular weight of 35.6kDa. (prospecbio.com)

Previous studies have indicated that KIN17 is involved in global genome repair, DNA replication, transcription and regulation of the cell cycle as part of a multi-protein complex. (spandidos-publications.com)

Bacterial effector binding to ribosomal protein S3 subverts NF-B function. (k-state.edu)
Solution NMR structure of the ribosomal protein RP-L35Ae from Pyrococcus furiosus. (rostlab.org)

Pegylated Human Prolactin was tested for its biological functionality in-vitro by inducing proliferation of Nb2 cells or Baf/3 cells that were stably transfected with Human Prolactin receptors, though its activity is lower than human Prolactin. (prospecbio.com)

Copper is required for many biological pathways, but sometimes it can harm the cell. (biorxiv.org)
Scholars@Duke publication: Modelling regulatory pathways in E. coli from time series expression profiles. (duke.edu)

To address this limitation, the construction of a series of genomically recoded organisms that are optimized for cellular protein production is described. (northwestern.edu)
In unicellular organisms like E. coli and yeast, the benefits of between potential cost and benefit, regulation can make the most a well-adapted regulatory system are readily quantified, as the difference to the long-term growth rate if the resource in question fitness of an individual can be estimated by its growth rate in is only available a similarly small fraction of the time. (lu.se)

Combining small-angle neutron and X-ray scattering for studying protein denaturation. (ibs.fr)

Abcam: antibodies, proteins, kits. (abcam.com)
Using multiple signal transduction schemes (including novel optical methods designed for amphiphiles), specific recognition antibodies, and a waveguide-based optical biosensor developed at Los Alamos National Laboratory, we have developed ultrasensitive detection methods for lipopolysaccharides (LPS), and protein biomarkers (Shiga toxin) of STEC in complex samples (e.g. beef lysates). (spie.org)

Assessing the conformational changes of pb5, the receptor-binding protein of phage T5, upon binding to its Escherichia coli receptor FhuA. (ibs.fr)
A method that couples limited proteolysis with a targeted proteomics workflow enables large-scale analyses of protein conformational changes directly in their biological matrices. (m2p-labs.com)

The first structures were of the glycerol 3-phosphate/phosphate exchanger GlpT and the lactose-proton symporter LacY, which served to elucidate the overall structure of the protein family and provided initial models for understanding the MFS transport mechanism. (wikipedia.org)
Assays of the temperature dependence of facilitated diffusion of sugar alcohols on a single set of PfAQP-reconstituted liposomes reveal that the activation energies for facilitated diffusion of xylitol and sorbitol are the same as that previously measured for glycerol transport in the aquaglyceroporin of Escherichia coli (5 kcal/mole). (chalmers.se)

Even though most of these changes are only slightly negative, at our current mutation rate, the recipes for the vital proteins in our bodies will eventually be corrupted to the point that we can't survive. (signsofthetimes.org.au)

The major facilitator superfamily (MFS) is a superfamily of membrane transport proteins that facilitate movement of small solutes across cell membranes in response to chemiosmotic gradients. (wikipedia.org)
The major facilitator superfamily (MFS) are membrane proteins which are expressed ubiquitously in all kingdoms of life for the import or export of target substrates. (wikipedia.org)

We also present a novel way of combining prior biological knowledge and current observations to improve the quality of analysis and to model interactions between sets of genes rather than individual genes. (duke.edu)
Exploration of the supramolecular interactions involving tris-dipicolinate lanthanide complexes in protein crystals by a combined biostructural, computational and NMR study. (ibs.fr)
On the other hand, recombinant therapeutical proteins have received a great deal of attention in recent years due to their advantages including low side effects, minimized cytotoxicity, high selectivity, and very low non-specific interactions [ 7 , 8 ]. (biomedcentral.com)

The findings show how a crucial enzyme in our blood, known as ADAMTS13, works like a pair of molecular scissors to carefully cut back the clotting effects of a key protein, von Willebrand factor (VWF). (nottingham.ac.uk)
Based on a paper we found that gives typical protein concentrations in E. coli [4] we chose to use four different enzyme concentrations in our model: 100, 500, 1000 and 100 000 enzymes per cell corresponding to low, medium, high and very high concentrations of enzyme. (igem.org)
The recombinant protein had enzymatic properties similar to those of the plant enzyme. (weedscience.com)
The lac operon of E. coli is but one example of how enzyme and transporter production can be made conditional on the presence of a nutrient to catabolize. (lu.se)
The initial velocity decreased at enzyme concentrations higher than 0.13 mg protein per 2.0 ml of incubation mixture. (who.int)

As an alternative strategy for the production of endotoxin-free biomolecules, a set of non-conditional E. coli derivatives was constructed that lack all outer membrane agonists for hTLR4/MD-2 activation. (dnsalias.com)

However, finding a generic method that is applicable to membrane proteins has been a challenge as the high hydrophobicity of membrane proteins and the presence of detergents essential for their solubilization interfere with fluorescence-based detections. (nih.gov)
Here the authors used MsbA (an adenosine triphosphate binding cassette transporter), CorA (a Mg(++) channel), and CpxA (a histidine kinase) as model proteins and show that DSLS is not sensitive to the presence of detergents or protein hydrophobicity and can be used to monitor thermodenaturation of membrane proteins, assess their stability, and detect ligand binding in a 384-well format. (nih.gov)
Small angle neutron scattering for the study of solubilised membrane proteins. (ibs.fr)
Change of dynamics of raft-model membrane induced by amyloid-beta protein binding. (ibs.fr)
Herein, we discuss this claim based on the available data for SARS-CoV-1 and -2 E and on new experiments performed using the untagged full-length E protein from SARS-CoV-2 in planar lipid membranes of different types, including those that closely mimic the ERGIC membrane composition. (bvsalud.org)
Numerous membrane-transport proteins are major drug targets, and therefore a key ingredient in pharmaceutical development is the availability of reliable, efficient tools for membrane transport characterization and inhibition. (chalmers.se)
Here, we present the use of evanescent-wave sensing for screening of membrane-protein-mediated transport across lipid bilayer membranes. (chalmers.se)

When blood vessels are damaged by a cut or by other types of vascular injury, VWF in blood plasma binds to the site of damage and unravels to form long protein strings that specifically capture specialised blood cells (platelets) to the site of injury. (nottingham.ac.uk)
The above biological processes have important roles in tumorigenesis, cancer development and chemoresistance in tumor cells. (spandidos-publications.com)
However, biopharmaceuticals manufactured in host cells contain host residual protein and DNA (hr DNA) as impurity and must be removed and quantified in the purified drug before it can be used in patients. (scirp.org)
High-affinity zinc uptake system protein mutant ( znuA mutant) showed reduced growth in zinc chelated medium, and failed to replicate in HeLa cells and mouse bone marrow-derived macrophages. (go.jp)
In order to determine the practicality of our solution, we are applying kinetic modeling to investigate how much time our engeneered ''E. coli'' cells will need to convert a certain amount of ammonia to nitrous oxide. (igem.org)
For ammonia concentrations typically encountered in wastewater modelling shows that our transformed ''E. coli'' cells will be able to do this within minuets. (igem.org)
Proteins self-organise to form patterns in living cells, which are essential for key functions such as cell division, communication and movement. (phys.org)

Biochemical, biophysical and biological assays using isolated amyloid β peptide, primary neurons and various established Alzheimer's disease nematode models showed that the selected macrocycles potently inhibit the formation of neurotoxic amyloid β peptide aggregates. (nature.com)

These proteins are vital to normal platelet adhesion and hemostatic function. (nottingham.ac.uk)

Isolates of E.coli (n=42) and K. pneumoniae (n=134) from blood culture collected during 2013-2015 were screened for carbapenemase production by using carba NP test and the presence of carbapenem resistant genes (KPC, IMP, VIM, NDM and OXA- 48 like). (omicsonline.org)

Proteases are enzymes that catalyze the hydrolysis of peptide bonds and play an important role in almost all biological processes. (bmrat.org)

Comparisons of NMR spectral quality and success in crystallization demonstrate that NMR and X-ray crystallography are complementary methods for small protein structure determination. (rostlab.org)
we have selected this protein for structure determination by X-ray crystallography. (rcsb.org)

Journal of Biological Chemistry , 262: 6515-6525, 1987. (igem.org)
Writing in the journal eLife , the researchers describe how they dissected the biological phenomenon of protein pattern formation into its main functional modules, and then rebuilt the process from the ground up in a completely new way. (phys.org)

She adds that while the patterns created by the new system are less regular than those formed by the native MinDE system, they are still sufficient for reproducing and studying basic biological processes. (phys.org)
Many technologies are used to characterize biological products, manufacturing processes, and raw materials. (bioprocessintl.com)

Additionally, bacterial virulence proteins utilize many molecular mechanisms that are also conserved among viruses of importance to biosecurity. (k-state.edu)
Our work provides a starting point for a modular and tunable experimental platform to design protein-based pattern formation from the bottom-up," says Petra Schwille, Ph.D., Director of the Department of Cellular and Molecular Biophysics at the Max Planck Institute of Biochemistry, and co-senior author alongside theoretical physicist Erwin Frey, from the Ludwig Maximilians University of Munich. (phys.org)
Pegylated Prolactin is mono-pegylated having a molecular mass of ~ 39 kDa, however under non-denaturing conditions it behaves as 220 kDa protein due to its increased hydrodynamic volume. (prospecbio.com)

It is demonstrated that the functional deactivation of nucleases (e.g., rne, endA) and proteases (e.g., lon) increases production of wild-type superfolder green fluorescent protein (sfGFP) and sfGFP containing two ncAAs up to ≈5-fold. (northwestern.edu)
Probing the functional segments, or 'motifs', of proteins has helped scientists identify the minimal ingredients needed for them to form biological patterns. (phys.org)
It can be reconstituted in the laboratory, allowing scientists to control and manipulate the functional elements needed for pattern formation via protein mutations. (phys.org)
To answer this, Glock and his colleagues created a minimalistic version of MinE, which plays an antagonistic role in the two-protein MinDE system, by dissecting the protein in a set of core functional motifs , guided by theoretical modelling. (phys.org)
But adding other functional motifs of MinE one at a time enabled the scientists to fully design new minimal pattern-forming protein mutants. (phys.org)
The model can now be used to study which functional features, regardless of a particular protein system, need to be combined to allow for self-organisation and pattern formation in biology. (phys.org)

Shiga toxin-producing Escherichia coli (STEC) poses a serious threat to human health through the consumption of contaminated food products, particularly beef and produce. (spie.org)

This conclusion is supported by the observation that Helicobacter pylori tatA will complement an E. coli tatA mutant, but not a tatB mutant. (ox.ac.uk)

Biological Chemistry , 390(9):863-873, 2009. (igem.org)

Those include its use as a critical starting material for transient transfection to produce both viral-vector constructs (e.g., lentivirus or adenoassociated virus) and transient proteins in development (e.g., monoclonal antibody). (bioprocessintl.com)

Anatomy8

Organisms24

Diseases6

Chemicals and Drugs109

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes81

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care4

Microorganisms3

Bacteria6

Periplasmic1

Peptides1

Assay2

Strain8

Bacterial Proteins1

Heterologous1

Soluble1

Vivo5

Molecules3

Enterotoxigenic2

Pathogen1

Extracellular5

Epithelial cells1

Ligands3

Codons2

Macromolecules1

Expression of proteins1

Bioinformatics1

Strains2

Structural5

Metabolism2

Functionally2

Kinases2

Synthesis1

Abundance1

Amino3

Genome1

Ribosomal protein2

Proliferation1

Pathways2

Organisms2

Denaturation1

Antibodies2

Conformational changes2

Glycerol2

Mutation1

Facilitator2

Interactions3

Enzyme5

Endotoxin-free1

Membrane7

Cells7

Assays1

Adhesion1

Isolates1

Peptide1

Crystallography2

Journal2

Processes2

Molecular3

Functional6

STEC1

Complement1

Chemistry1

Viral1