AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaTechnology, Industry, AgricultureInformation ScienceHealth Care
CentrifugationCentrifugation, Density GradientCentrifugation, ZonalCentrifugation, IsopycnicCell FractionationModels, BiologicalMetrizamideMolecular WeightMicroscopy, ElectronBiological ProductsSubcellular FractionsElectrophoresis, Polyacrylamide GelKineticsPovidoneBiological AssayMolecular Sequence DataCell MembraneUltracentrifugationCell SeparationBiological TherapyHypergravityCell LineChromatography, GelFiltrationAmino Acid SequenceCattleProteinsTritiumCells, CulturedBiological MarkersLiverProtein BindingTime FactorsSolubilityBiological WarfareSucroseHydrogen-Ion ConcentrationRabbitsMethodsDNATemperatureBiological ProcessesEscherichia coliSilicon DioxideChromatographyBase SequenceOrganoidsChromatography, High Pressure LiquidGene Expression ProfilingMacromolecular SubstancesCesiumDetergentsAlgorithmsAmino AcidsComputational BiologyStructure-Activity RelationshipMembrane ProteinsBiological TransportNucleotidasesMembranesPeptidesBinding SitesAcid PhosphataseRNA, MessengerLysosomesPest Control, BiologicalMutationSignal TransductionDiatrizoateSpermatozoaCell NucleusRNAChemical PrecipitationMicrosomesPolyethylene GlycolsMolecular StructureErythrocytesRecombinant ProteinsElectrophoresisPhospholipidsSoftwareCarbon IsotopesCarbohydratesChemistryRats, Inbred StrainsChromatography, Ion ExchangeCytoplasmColloidsSpecies SpecificityChromatography, DEAE-CelluloseFreezingChemical PhenomenaChromatography, AffinityChemistry Techniques, AnalyticalCell-Free SystemBacteriaModels, MolecularProtein ConformationCytoplasmic GranulesComputer Simulation
Sucrose density gradientCentrifugeDensity-gradientElectrophoresisFiltrationParticlesSeparatesMitochondriaSpecimensSeparationTubesSafety cabinetPurifyDiagnosticsNucleic acidsHazardsLaboratoriesConsistentProteinSciencesProcessesSamplesAnalysisSoilInvolvesTechniquesAgriculturalExtractsChemicalEnzymeProcessMethodConclusionsProtocolsTechniqueCellStudyResearchReferSampleProductsSolutionVarietyForceMethodsSystemsCollectionApplications
Sucrose density gradient2
  • The prerequisite was the generation of specific antisera directed against cyanelle SecY that allowed immunodetection of the protein on SDS gels from both membrane types separated by sucrose density gradient floatation centrifugation. (biomedcentral.com)
  • The rAU if present would melt sharply and be detectable and would show up as 5-6 S material in sucrose density gradient centrifugation of the hepatopancreas RNA after T1 treatment. (caltech.edu)
Centrifuge2
  • Use sealed centrifuge rotors or sample cups, if available, for centrifugation. (cdc.gov)
  • The goal for this scenario is for the user to select the correct tubes, properly balance, and load the centrifuge during the centrifugation process. (cdc.gov)
Density-gradient3
Electrophoresis2
Filtration4
Particles4
  • Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. (wikipedia.org)
  • The particles' settling velocity in centrifugation is a function of their size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. (wikipedia.org)
  • 2) The principle of the centrifugation technique is to separate the particles suspended in liquid media under the influence of a centrifugal field. (toppr.com)
  • In centrifugation, centrifugal force is used as the driving force for the separation of particles. (toppr.com)
Separates1
  • Centrifugation is a process which separates or concentrates materials suspended in a liquid medium. (toppr.com)
Mitochondria2
Specimens3
  • Microbiology and pathology laboratories performing diagnostic tests on stool or respiratory specimens should handle potential SARS-CoV specimens using standard Biosafety Level (BSL)-2 work practices in a Class II biological safety cabinet. (cdc.gov)
  • Any procedure with the potential to generate fine-particulate aerosols (e.g., vortexing or sonication of specimens in an open tube) should be performed in a biological safety cabinet (BSC). (cdc.gov)
  • It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (bvsalud.org)
Separation2
  • The extraction of nucleic acids from biological material requires cell lysis, inactivation of cellular nucleases, and separation of the desired nucleic acid from cellular debris. (pharmiweb.com)
  • We develop novel lab-on-a-chip technology for separation, enrichment and manipulation of cells and biological microparticles. (lu.se)
Tubes4
  • We aimed comparison and stability of nine biochemical analytes after centrifugation using Barricor™ plasma tubes with mechanical separator vs standard Vacutainer® lithium heparin tubes. (biochemia-medica.com)
  • By using Barricor™ tubes and prompt centrifugation, supplemental analysis or re-analysis for eight analytes including alanine aminotransferase, alkaline phosphatase, C-reactive protein, high sensitivity troponin T, lactate dehydrogenase, NT-pro BNP, potassium and sodium could be performed within 72 h of specimen collection. (biochemia-medica.com)
  • According to the manufacturer, the quality of plasma is improved, the centrifugation time is shorter (3 min vs. 10 min with BD Vacutainer® lithium heparin tubes) and post centrifugation analyte concentrations are more stable in samples that have been stored in the primary tube. (biochemia-medica.com)
  • Draw sufficient blood in plain red top tubes (preferred for most biological monitoring) or serum separatortubes (SST) to yield required serum volume (see specimen requirements for the analysis being ordered). (pactox.com)
Safety cabinet2
  • Identify major parts of a Class II biological safety cabinet (BSC) and implement proper practices for its safe and effective operation. (cdc.gov)
  • 4. Air dry the filter papers thoroughly in the back of the Class II Biological Safety Cabinet. (who.int)
Purify1
  • Clarifying cell culture broth is the first downstream unit operation in an elaborate sequence of steps required to purify a biological therapeutic. (bioprocessintl.com)
Diagnostics1
  • There are alternatives to centrifugation such as microfluidic technology, which has been widely studied for various applications in biological and chemical analysis, point-of-care testing and diagnostics, and clinical analysis. (fau.edu)
Nucleic acids1
  • Biological studies require purified and isolated nucleic acids as the first step and in all recombinant DNA techniques. (pharmiweb.com)
Hazards1
  • understand the basics of safety and biosafety management issues when working with toxic chemicals, biological samples, physical hazards, and when interacting with patients. (who.int)
Laboratories2
  • Containment of biological agents and infectious substances is an essential element in maintaining a safe environment for biomedical and microbiological laboratories, Biosafety Levels labs, and vaccine production facilities. (beckman.com)
  • The LRN is a network of 160 domestic and international laboratories that work around the clock to provide rapid testing of biological and chemical threat agents and support for other public health emergencies. (cdc.gov)
Consistent1
Protein1
  • For biological systems, Q peaks were first observed in protein from different perspectives [3,7,9,13,15,21-23] , the detailed molec- powders and attributed to 1H-14N spin pairs [9], which were also ular mechanism of the ubiquitous 1H-14N Q peaks in biological sys- thought to be of critical importance for the background 1H relaxa- tems has not been established. (lu.se)
Sciences2
  • DNA damage is one of the more important characteristics of sperm, because higher levels of damage are associated with lower pregnancy rates, early loss of pregnancy, and increased disease rates of offspring who are conceived through assisted reproductive technologies," said Waseem Asghar, Ph.D., assistant professor in the Department of Electrical Engineering & Computer Science, and the Department of Biological Sciences at FAU. (fau.edu)
  • Prerequisite: Undergraduate or graduate degree in chemical engineering or in the biological or physical sciences. (rutgers.edu)
Processes3
  • Many technologies are used to characterize biological products, manufacturing processes, and raw materials. (bioprocessintl.com)
  • Recently, identification of metabolites involved in the biotransformation of phytochemicals by intestinal microflora has been suggested as a potentially effective means to determine which compounds are active in living systems and to gain a better understanding of how herbs affect biological processes [ 11 , 12 ]. (hindawi.com)
  • After going through these processes, the cells are concentrated and separated by centrifugation for 14 minutes to obtain a cell concentrate. (who.int)
Samples5
  • These bottles are for low to moderate speed centrifugation of biological and chemical samples. (usplastic.com)
  • In an effort to determine the biological availability of certain metals, samples of coal were mixed with serum, lung washings (LW), and saline. (cdc.gov)
  • We subjected 5 mL of blood to centrifugation for 1 hour at 1,000 rpm and then stored the plasma at −80°C. Blood samples from other patients were used as controls. (cdc.gov)
  • Circulation paths should be designed in such a way that contact between the public and biological materials can occur only in the rooms where patient samples are collected. (who.int)
  • Circulation pathways of biological samples between different sections of the laboratory--These pathways should be assessed for the purpose of minimizing contamination risks. (who.int)
Analysis1
  • miRNA microarray profiling was performed using Lianchuan biological small RNA sequencing analysis. (hindawi.com)
Soil1
  • Students also gain valuable experience through participation in professional organizations such as the American Society of Agricultural and Biological Engineers, the UNL Soil and Water Resources Club, the Quarter-Scale Tractor Club, the Fountain Wars Club, the Nebraska Society of Professional Engineers, and the Society for Women Engineers. (unl.edu)
Involves1
  • The most commonly used or conventional method for assisted reproductive technologies involves a technique called centrifugation. (fau.edu)
Techniques2
  • Sperm sorting methodologies based on microfluidic procedures are a valuable option since these techniques eliminate the damaging centrifugation steps. (fau.edu)
  • To this end database search engines were rapidly developed after the introduction of ionization techniques for biological mass spectrometry [1-5]. (lu.se)
Agricultural1
  • Agricultural engineering (AGEN) is one of two engineering degree programs offered in the Department of Biological Systems Engineering. (unl.edu)
Extracts1
  • Within this work, and considering the discussed background, another D. tertiolecta clone (CCMP 1320) was selected and tested against several cancer cell lines, and a bioactivity-guided fractionation approach allowed to identify the source of the biological activity observed within the extracts of this green alga. (frontiersin.org)
Chemical3
  • 16:155:513 (F) Fundamentals of Nanoscale Thermodynamics and Transport (3) Introduction to theoretical and multiscale simulation methods applied to thermodynamics and transport in nanoscale chemical and biological systems, including nanoparticles and nanocomposites, porous materials, nanostructured colloids and surfaces, self-assembled surfactant and polymeric systems, lipid bilayers, and cell membranes. (rutgers.edu)
  • CE suffers from a poor high resistance to biological and chemical degradations [4-6]. (lu.se)
  • The most comprehensive collection of chemical and biological modi- fications being relevant to mass spectrometry can be found in UniMod [12]. (lu.se)
Enzyme1
  • However, it was an easily estimated enzyme, very sensitive for detecting and assessing acute damage to liver cells, and could be a useful adjunct or alternative to the serum transaminasess Part R involved the study of the enzyme, p-glucuronidase, in various biological material taken from patients with carcinoma of the cervix uteri. (gla.ac.uk)
Process1
  • The light will cause possible biological stains to change color or fluoresce, assisting in the location process. (ojp.gov)
Method3
  • Centrifugation is also the most common method used for uranium enrichment, relying on the slight mass difference between atoms of U-238 and U-235 in uranium hexafluoride gas. (wikipedia.org)
  • In 2012, we isolated R. typhi from a human patient in southeastern Mexico by using a simple and effective method, an adaptation of the centrifugation shell vial method to cell culture plates. (cdc.gov)
  • In such kind of cases, centrifugation method is useful. (toppr.com)
Conclusions1
  • Designing a biological monitoring program to assess community exposure to chromium: conclusions of an expert panel. (cdc.gov)
Protocols1
  • Introduction: The urgent need for studies using standardized protocols to evaluate the real biological effects of PRP has been emphasized by several authors. (bvsalud.org)
Technique1
Cell2
  • Cell debris was removed by centrifugation. (bio-connect.nl)
  • Biological/Vaccine, Stem Cell, Procedure/Surgery : After measuring the patient s wound size at the initial visit, the amount of bone marrow collected is 30cc if less than 10cm2, 60cc if less than 20cm2, and 120cc if more than 20cm2, depending on the wound size. (who.int)
Study3
  • With the advancement of next-generation sequencing and mass spectrometry, there is a growing need for the ability to merge biological features in order to study a system as a whole. (rsc.org)
  • Whether or not these new conveyors of biological information contribute to the acquisition and/or maintenance of the altered endothelial phenotype is unexplored in PH and requires further study. (ersjournals.com)
  • Temple, NJ & El-Khatib, SM 1983, ' High fat diets and mouse colon mucosal membranes: A centrifugation study ', Cancer Letters , vol. 21, no. 2, pp. 225-231. (athabascau.ca)
Research1
  • A central area of our research is to use acousticforces (acoustophresis) in micrscale to manipulate biological mater. (lu.se)
Refer2
  • please refer to individual test requirements or to the Biological Exposure Index (BEl) section. (pactox.com)
  • In addition, you may wish to refer to the "Biological Exposure Indices (BEI) Testing" section of this Directory of Services for recommended times of collection. (pactox.com)
Sample2
  • A gene present on the X and Y sex chromosomes that is used in DNA identification testing to determine the gender of the donor of the DNA in a biological sample. (ojp.gov)
  • If the red color is due to hemoglobinuria, the urine sample remains clear red after centrifugation. (medscape.com)
Products1
Solution1
Variety1
  • 3 MHz has been observed in a variety of aqueous biological systems. (lu.se)
Force1
  • a rough guide to the 'g' force exerted at this speed is to multiply the centrifugation radius by a factor of 10, so a radius of 160 mm gives approximately 1600 x g. (wikipedia.org)
Methods1
  • Microfluidic based technologies have surfaced as a novel substitute to conventional methods that use centrifugation," said Asghar. (fau.edu)
Systems2
  • PBPK models based on corrected abundances will more closely resemble biological systems, and facilitate development of more meaningful IVIVE scaling factors, producing more accurate quantitative predictions of drug disposition. (aspetjournals.org)
  • The Department of Biological Systems Engineering is located in Chase Hall on East Campus. (unl.edu)
Collection1
Applications1
  • Recently, gold nanorods (GNRs) have attracted growing interest in biological/biomedical applications. (springer.com)