• Using enzyme-linked immunosorbent assay (ELISA) to enrich tissue homogenates for cholinesterase while depleting the sample of sulfhydryl groups eliminates unwanted interactions with DTNB, making it possible to measure low cholinesterase activity in biological samples. (sigmaaldrich.com)
  • Here we describe a new capture enzyme-linked immunosorbent assay (cELISA) based on monoclonal antibodies specific for lipopolysaccharide (LPS) of Francisella tularensis subsp. (asm.org)
  • General significance: In vitro assays offering low volume sampling and rapid analysis in a high-throughput manner are of great interest in a wide range of single-cell applications. (diva-portal.org)
  • In vitro assays with rat mesenchymal stem cells (rMSCs) and rat endothelial progenitor cells (rEPCs) also showed that rMSCs attached and proliferated on the materials influenced by the calcium content in the environment. (rsc.org)
  • Many parallel biochemical assays rely on thin aqueous films to spread a reactant solution over a wide area decorated with multiple distinct substrates. (cornell.edu)
  • Among these, we identified a novel negative GSR feedback regulator, termed NepR2, on the basis of gene reporter assays, phenotypic analyses, and biochemical assays. (nature.com)
  • Development of Asian carp liver cell lines for biological assays Justine Nelson (Tammy Clark) Abstract The Asian carp are an invasive species that have a high probability of causing ecological and economic effects to the fishing industry where inhabitants are found. (viterbo.edu)
  • Although there exists a current demand for higher sensitivity, the method of C-peptide quantitative analysis by Isotope-dilution assays allows for greater specificity as compared to current immunoassay methods. (umsystem.edu)
  • The general approach of most bioassays is to perform a dilution assay, which measures the biological responses at several doses. (bioassay.de)
  • A key assumption of a dilution assay is that the active component follows the same principle of activity in standard and in sample preparation. (bioassay.de)
  • Dilution assays can be direct or indirect. (wikipedia.org)
  • In a direct dilution assay the amount of dose needed to produce a specific (fixed) response is measured, so that the dose is a stochastic variable defining the tolerance distribution. (wikipedia.org)
  • Conversely, in an indirect dilution assay the dose levels are administered at fixed dose levels, so that the response is a stochastic variable. (wikipedia.org)
  • For a mathematical definition of a dilution assay an observation space U {\displaystyle U} is defined and a function f : U → R {\displaystyle f:U\rightarrow \mathbb {R} } so that the responses u ∈ U {\displaystyle u\in U} are mapped to the set of real numbers. (wikipedia.org)
  • Of notable importance in this respect is the theory of Generalized Linear Models with which a wide range of dilution assays can be modelled. (wikipedia.org)
  • Additionally, we have developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. (frontiersin.org)
  • Additionally, slope-ratio and dichotomous assays (quantal response assays) are available as well. (bioassay.de)
  • They also discussed measures needed to assess iPSC-derived cell-type fidelity and physiological relevance to their in vivo counterparts, along with current progress and need for improved cell assay technologies. (nih.gov)
  • In preclinical cancer research, bioluminescence imaging with firefly luciferase and D -luciferin has become a standard to monitor biological processes both in vitro and in vivo . (nature.com)
  • In vivo assays showed that hybrid composite PLA-ORMOGLASS fibers were able to promote the formation of blood vessels. (rsc.org)
  • These characteristics made MN assay very popular for screening of the effects of various genotoxic agents in vitro and in vivo. (ijrr.com)
  • The combination of mAbs most suitable for an antigen capture assay format was identified and an immunoassay using the Luminex xMAP technology was developed. (biomedcentral.com)
  • These probes are readily used directly under natural conditions in biofluids and are often directly developed and optimized for cellular assays, thus leaving little doubt about their specificity and utility under biologically relevant conditions. (mdpi.com)
  • Luciferase -reporter assays are widely used to monitor the cellular events related to transduction and gene expression regulated by specific cascades, such as PRL/Jak2/Stat 5 pathway. (bvsalud.org)
  • Specifically, despite the human body's endogenous receptors, hormones, and other neurotransmitters, pharmacologists utilize assays in order to create drugs that are selective, or mimic, the endogenously found cellular components. (wikipedia.org)
  • Zhang M, Sternberg MR, Pfeiffer CM. Harmonizing the calibrator and microorganism used in the folate microbiological assay increases the comparability of serum and whole blood folate results in a CDC round-robin study. (cdc.gov)
  • Perhaps there is no perfect assay for sensitively and accurately quantifying H 2 O 2 as well as other ROS in cells, wherein numerous potential reactants are present to interfere with the reliable measurement of the specific ROS. (nih.gov)
  • Since the measurement of cell viability was based on the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to formazan crystal by mitochondrial dehydrogenases [ 11 ], in the current study we revisited our previous results, checking for a possible interference of Rottlerin in the MTT assay. (biomedcentral.com)
  • A history of biological standardization : the characterization and measurement of complex molecules important in clinical and research medicine : contributions from the UK, 1900-1995 : what, why, how, where, and by whom / a personal account by D.R. Bangham. (who.int)
  • The third problem is analytical and biological variance, which deals with deviations in measurement and in composition. (wikibooks.org)
  • Salimi M, Mozdarani H. Different aspects of cytochalasin B Blocked micronucleus cytome (CBMN cyt) assay as a comprehensive measurement tool for radiobiological studies, biological dosimetry and genome instability. (ijrr.com)
  • The NIMH Division of Neuroscience and Basic Behavioral Science convened a workshop of scientists involved in several ongoing NIMH-funded research efforts involving human induced pluripotent stem cells (iPSCs) or similar reprogrammed cell assays. (nih.gov)
  • Lack of biological assays to measure many of these contaminants in tissue specimens, the high cost of using these assays in large epidemiologic studies when they were available, and challenges related to the interpretation of the results when exposures were measured after the adverse reproductive event occurred limit the conclusions that can be drawn from these investigations. (thefreedictionary.com)
  • Employ Arxspan Assay to provide study coordinators with an integrated view of study activity and assay data as reported by research team(s), and empower individual researchers to collaborate. (bruker.com)
  • 1. Determination of the strength or biological activity of a substance, such as a drug, by comparing its effects with those of a standard preparation on a test organism. (thefreedictionary.com)
  • Early estimates of CCK-like activity in blood were based on biological assays such as pancreatic secretion or gallbladder contraction. (thefreedictionary.com)
  • This study was designed to investigate impact of tannins on in vitro ruminal fermentation parameters as well as relationships between concentration and in vitro biological activity of tannins present in tree fruits. (scielo.org.za)
  • Correlation between in vitro biological activity and phenolic concentration was determined. (scielo.org.za)
  • Moreover, TINA was applied to investigate the influence of chemical processing on the activity of biological IN, in particular the effects of oxidation and nitration reactions. (atmos-meas-tech.net)
  • Our results demonstrated that this IFN-γ based high-throughput assay for screening of natural products for NK cell tumoricidal activity is a simple, economic and reliable method. (biomedcentral.com)
  • Precursor forms of IL-18 and IL-1β lack a signal peptide and they require cleavage by caspase-1 (IL-1β-converting enzyme) for their maturation, and hence for their biological activity ( 15 , 16 , 17 , 18 ). (jimmunol.org)
  • The biological role of miR-1 in controlling MET expression and biological activity was assessed in colon cancer cells either by its forced expression or by AntagomiR-mediated inhibition. (aacrjournals.org)
  • In consequence, the biological activity of EOs from the leaves and fruits of Schinus areira (Anacardiaceae) and the leaves of Thymus vulgaris (Lamiaceae), Aloysia polystachya and Aloysia citriodora (Verbenacea) were evaluated against the eggs and adults of P. humanus capitis by fumigant and contact toxicity bioassays. (nih.gov)
  • The basic design of bioassays was developed in the nineteenth century, but test species were used as an assay of exposure to some stimulus well before that time. (nap.edu)
  • It includes information on all aspects of drug research and development, from drug discovery to biological assays , pharmacokinetics, clinical trials, and post-market surveillance. (thefreedictionary.com)
  • For efficient data analysis and clinical genotyping report generation, DeepChek® SingleRound assays are bundled with the ViroScore® and DeepChek® software, both CE-marked for IVD use, for Sanger and NGS, respectively. (laraza.com)
  • The WHO recommends the microbiologic assay for assessing folate status in population surveys (WHO, 2015) and this assay is amenable for laboratory capacity building in low-resource settings (Pfeiffer et al. (cdc.gov)
  • Parameters of importance for optimization of a cell based assay will be tested empirically in the laboratory and their significance calculated using Z'. During the course a small-scale screen will be executed. (umu.se)
  • In the cytogenetic laboratory of the BfS in Munich/ Neuherberg , individuals who have or may have been exposed to an increased radiation dose have the possibility to use the biological dosimetry service for dose assessment under specific requirements. (bfs.de)
  • A method for performing time resolved homogeneous assays using a long-lifetime luminescent dye as a donor. (patentgenius.com)
  • 2. The method of claim 1 wherein said biological fluid is an oral fluid. (patentgenius.com)
  • 2. The application of a progressively increasing dose method to the biological assay of epinephrine may be an improvement upon the U.S.P.XI procedure. (aspetjournals.org)
  • A method for the quantitation of the effects on a biological system by its exposure to a substance, as well as the quantitation of the concentration of a substance by some observable effect on a biological system. (thefreedictionary.com)
  • Statistical method in biological assay / David J. Finney. (who.int)
  • A fluorometric method for the estimation of the vitamin B1 content in food and biological materials is described. (semanticscholar.org)
  • The implementation of this method in the study of complex biological systems would have an immediate impact in academic and medical research. (montana.edu)
  • Together with an improvement in the LOD of at least 1 order of magnitude compared to that of conventional assays, this suggests that the method will become useful in furthering our understanding of the role of PLA2 in health and disease and in detecting the pharmacodynamic effects of PLA2-targeting drug candidates. (chalmers.se)
  • Biological dosimetry is an internationally approved method to quantify the exposure and to perform a dose assessment following a suspected radiation overexposure. (bfs.de)
  • The method is internationally considered the gold standard for biological dosimetry. (bfs.de)
  • In some aspects, a method is provided for imaging assay beads. (justia.com)
  • The method further including driving a light emitter to project light through the wells, recording an output signal of at least one of absorbance and fluorescence, and converting the output signal to a value indicative of a reaction of a biological sample within each of the plurality of wells. (justia.com)
  • Multiple components of the overall assay system - from instruments to incubation media - need to be within defined limits to permit execution of a valid assay, so they are tested for suitability either before or during the assay. (bioprocessintl.com)
  • Biological responses were more highly correlated with Cd concentrations evaluated using bioavailable methods than with total concentrations. (lancs.ac.uk)
  • All methods are suitable for analysing the results obtained by several biological assays. (bioassay.de)
  • All of these methods were extensively used for various purposes but among them micronucleus (MN) assay was found more practical because of ease of scoring, potential for automation as well as being nearly as sensitive as other procedures. (ijrr.com)
  • In the growing field of biological assays there has been demonstrated need for luminescent dyes that emit outside of the visible range where traditional fluorescence measurements suffer from high levels of interference. (umich.edu)
  • The i-STAT® System comprises one or more disposable testing devices, operating in conjunction with a hand-held analyzer, for performing a variety of measurements on biological specimens such as blood. (justia.com)
  • The Canadian team of organic chemists and enzymologists developed drug design motifs, and pursued preparative chemistry, enzymology, molecular biology, and tissue culture studies that were interfaced with biological assays and pharmacokinetic studies carried out in Syntex, Palo Alto, CA. (thefreedictionary.com)
  • The biological assays measured effects on chromosomes (micronuclei and sister-chromatid exchange) and on an enzyme, O 6 -Alkylguanine DNA Alkytransferase (ACT) that repairs DNA, (deoxyribonucleic acid), the genetic material of which the chromosomes are made. (cdc.gov)
  • The aim of this study was to develop a rapid and sensitive immunological assay to detect all Brucellae with 'smooth' LPS, particularly B. melitensis and B. abortus . (biomedcentral.com)
  • A variety of natural, derivatized and designed hyperpolarized probes has emerged for diverse biological studies including assays of intracellular reaction progression, pathway kinetics, probe uptake and export, pH, redox state, reactive oxygen species, ion concentrations, drug efficacy or oncogenic signaling. (mdpi.com)
  • This work sets out to investigate biological responses of Eisenia fetida to Cd, based on the bioavailable rather than total concentration of Cd, in soils. (lancs.ac.uk)
  • Antioxidant system responses in E. fetida to Cd were measured as biological endpoints. (lancs.ac.uk)
  • Cd concentration measured using DGT and CaCl2 extraction provided the narrowest ranges of lowest observed effect concentration (LOEC) values based on biological responses, indicating the potential use of these measurements in management of soil quality and setting soil remediation standards. (lancs.ac.uk)
  • Terbium and Rhodamine as Labels in a Homogeneous Time-resolved Fluorometric Energy Transfer Assay of the .beta. (patentgenius.com)
  • Time-resolved Fluorescence Resonance Energy Transfer Assay for Point-of-Care Testing of Urinary Albumin" (Title) Clin. (patentgenius.com)
  • This data taken together with the long luminescence lifetimes and narrow emission bands associated with lanthanides make this technology an excellent tool for time resolved, low-background, multiplexed assays and biomedical imaging. (umich.edu)
  • 10 The suitable biological performance of these scaffolds was mainly attributed to their stiffness and the ions released from the glass, in particular calcium ions, as well as the intrinsic surface properties of the materials. (rsc.org)