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  • dilution assay
  • The general approach of most bioassays is to perform a dilution assay, which measures the biological responses at several doses. (bioassay.de)
  • A key assumption of a dilution assay is that the active component follows the same principle of activity in standard and in sample preparation. (bioassay.de)
  • In a direct dilution assay the amount of dose needed to produce a specific (fixed) response is measured, so that the dose is a stochastic variable defining the tolerance distribution. (wikipedia.org)
  • Conversely, in an indirect dilution assay the dose levels are administered at fixed dose levels, so that the response is a stochastic variable. (wikipedia.org)
  • For a mathematical definition of a dilution assay an observation space U {\displaystyle U} is defined and a function f : U → R {\displaystyle f:U\rightarrow \mathbb {R} } so that the responses u ∈ U {\displaystyle u\in U} are mapped to the set of real numbers. (wikipedia.org)
  • Probes
  • The hyperpolarized probes are produced ex situ , introduced into biological systems and detected with high sensitivity and contrast against background signals using high resolution NMR spectroscopy. (mdpi.com)
  • A variety of natural, derivatized and designed hyperpolarized probes has emerged for diverse biological studies including assays of intracellular reaction progression, pathway kinetics, probe uptake and export, pH, redox state, reactive oxygen species, ion concentrations, drug efficacy or oncogenic signaling. (mdpi.com)
  • These probes are readily used directly under natural conditions in biofluids and are often directly developed and optimized for cellular assays, thus leaving little doubt about their specificity and utility under biologically relevant conditions. (mdpi.com)
  • Hyperpolarized molecular probes for biological NMR spectroscopy enable the unbiased detection of complex processes by virtue of the high spectral resolution, structural specificity and quantifiability of NMR signals. (mdpi.com)
  • Here, we provide a survey of strategies used for the selection, design and use of hyperpolarized NMR probes in biological assays, and describe current limitations and developments. (mdpi.com)
  • Lerche, M.H. Hyperpolarized NMR Probes for Biological Assays. (mdpi.com)
  • quantitative
  • This assay provides both a qualitative (visible observation of colour gradient) for screening purposes, or quantitative measurement (spectrophotometry) for calculation of commonly accepted metrics. (wikipedia.org)
  • In the quantitative assay, the dose-response (colour production linked to beta-galactosidase production) of a compound is plotted, with the slope of the initial linear region used as a universal parameter, the SOS-inducing potency (SOSIP), which reflects the ability of a compound to induce the SOS response (measured indirectly through production of beta-galactosidase and the breakdown of a lactose analog). (wikipedia.org)
  • Additionally
  • Additionally, we have developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. (frontiersin.org)
  • simultaneously
  • Since the chemical tested may inhibit protein synthesis at higher concentrations, which would lead to an underestimation of B-galactosidase induction, alkaline phosphatase is assayed simultaneously with β-galactosidase in order to scale the data to survivability of the cells. (wikipedia.org)
  • compound
  • The test serves as a quick and convenient assay to estimate the carcinogenic potential of a compound because standard carcinogen assays on mice and rats are time-consuming (taking two to three years to complete) and expensive. (wikipedia.org)
  • hormone
  • In 1949, he began post-graduate studies at the University of Edinburgh, returning to Sri Lanka in 1952 with a PhD. His doctorate thesis was a study of biological assays of cortical hormone and their application. (wikipedia.org)
  • activity
  • 1. Determination of the strength or biological activity of a substance, such as a drug, by comparing its effects with those of a standard preparation on a test organism. (thefreedictionary.com)
  • Early estimates of CCK-like activity in blood were based on biological assays such as pancreatic secretion or gallbladder contraction. (thefreedictionary.com)
  • and (g) determining a spatial pattern of the biological target abundance, expression and/or activity in the sample based on the analysis of step (f). (patentsencyclopedia.com)
  • In addition to the bovine and porcine tissue from which pharmaceutical-grade heparin is commonly extracted, it has also been extracted and characterised from: Turkey Whale Dromedary camel Mouse Humans Lobster Fresh water mussel Clam Shrimp Mangrove crab Sand dollar Atlantic salmon Zebra fish The biological activity of heparin within species 6-11 is unclear and further supports the idea that the main physiological role of heparin is not anticoagulation. (wikipedia.org)
  • tissue
  • Lack of biological assays to measure many of these contaminants in tissue specimens, the high cost of using these assays in large epidemiologic studies when they were available, and challenges related to the interpretation of the results when exposures were measured after the adverse reproductive event occurred limit the conclusions that can be drawn from these investigations. (thefreedictionary.com)
  • method
  • A method for the quantitation of the effects on a biological system by its exposure to a substance, as well as the quantitation of the concentration of a substance by some observable effect on a biological system. (thefreedictionary.com)
  • complex
  • In analogy to digital microelectronics, these basic instructions can be combined and reused within hierarchical design structures so that complex procedures (e.g. chemical synthesis or biological assays) can be built up step-by-step. (wikipedia.org)
  • provides
  • Well Characterized Biologics & Biological Assays is the only event in the industry that provides the opportunity to learn from and interact directly with the regulators who will review and approve your CMC programs. (knect365.com)
  • time
  • Terbium and Rhodamine as Labels in a Homogeneous Time-resolved Fluorometric Energy Transfer Assay of the .beta. (patentgenius.com)
  • This data taken together with the long luminescence lifetimes and narrow emission bands associated with lanthanides make this technology an excellent tool for time resolved, low-background, multiplexed assays and biomedical imaging. (umich.edu)
  • range
  • The biological material in which the effect is measured can range from subcellular components and microorganisms to groups of animals. (thefreedictionary.com)
  • Of notable importance in this respect is the theory of Generalized Linear Models with which a wide range of dilution assays can be modelled. (wikipedia.org)
  • technology
  • This project leverages recent and dramatic advances in DNA sequencing technology that have transformed the process of rapid, accurate, and cheap assays of community biodiversity. (harteresearchinstitute.org)
  • wide
  • Many parallel biochemical assays rely on thin aqueous films to spread a reactant solution over a wide area decorated with multiple distinct substrates. (cornell.edu)