Biological and chemical assays. Medical search. Frequent questions

Standard protein purification and characterization techniques, enzyme activity assays, as well as immunoassays (ELISA) will also be taught. (uaeu.ac.ae)
ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. (immpact-international.org)
ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. (immpact-international.org)

Unlike traditional biochemical assays, cell-based assays are more physiologically relevant and can assess compound characteristics simultaneously. (sigmaaldrich.com)

Discover a high throughput assay using organoids and Millicell ® Microwell plates. (sigmaaldrich.com)
Our high throughput assay protocol evaluates the killing capacity to colorectal tumor spheroids and enables patient-specific assays. (sigmaaldrich.com)
Can be readily automated as a high-throughput assay for thousands of samples per day. (immpact-international.org)

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue. (sigmaaldrich.com)

Cell viability and cytotoxicity assays are used usually for screening of drugs and cytotoxicity tests of chemicals. (benthamscience.com)
Cellular assays, or cell-based assays, can be used in both biomedical research and drug-discovery screening applications to efficiently quantify cytotoxicity, biological activity, biochemical mechanisms and off-target interactions. (sigmaaldrich.com)
The Biological Response section includes ecotoxicity (bacterial and daphnia), mammalian cytotoxicity (HepG2 cells), and genotoxicity (Ames) assays. (nih.gov)
The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)

Cell viability and proliferation assays can be effectively used to evaluate cell health. (sigmaaldrich.com)
Cell viability assay also revealed that the extracts could safely be used in concentrations of 125 µg/mL. (bvsalud.org)

In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. (immpact-international.org)
In this context, this work aimed at the development native to Brazil and found in Central and South American and chemical characterization of glycolic extracts of countries (Aragão et al . (bvsalud.org)

Comprehensive molecular, cellular and systemic-level assays all support a high level of selectivity. (nature.com)
We do so by demonstrating selectivity across comprehensive molecular, cellular, and systemic assays. (nature.com)
Such probes may be useful to investigate the biological role of CB2 receptors in cellular assays. (nih.gov)
Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq , " Evaluation of Cellular Processes by In Vitro Assays ", Bentham Science Publishers (2018). (benthamscience.com)

Can the 'standard deviation' or the assay itself be calculated from these data, as if they were direct, repeated measurements? (physicsforums.com)
The low relative standard deviation values of inter-day and intra-day assays highlighted the good reproducibility of the proposed method for assay of nevirapine. (bgu.ac.il)

The approval was based on "comprehensive review of scientific evidence," including "comparisons of the products on an analytical level using an extensive battery of chemical and biological tests and biological assays that confirmed similarity in the structural and functional features of Wezlana and Stelara (including those known to impact safety and efficacy), and comparative human pharmacokinetic data, clinical immunogenicity data, and other clinical safety and effectiveness data," the FDA said. (medscape.com)

This course will cover the structure and function of different biological molecules, including proteins, carbohydrates, lipids and nucleic acids. (uaeu.ac.ae)

Through a combination of structure-based virtual and high-throughput screening, we assayed more than 10,000 compounds-including approved drugs, drug candidates in clinical trials and other pharmacologically active compounds-as inhibitors of M pro . (nature.com)
To characterize the enzymatic activity of SARS-CoV-2 M pro and to carry out high-throughput screening of inhibitors, we developed a fluorescence resonance energy transfer assay. (nature.com)

Categorize Biological Membranes And Various Mechanisms Of Transport Of Molecules Through Membranes. (uaeu.ac.ae)
In one run of the assay M molecules are tested, generating 2 M N data points. (physicsforums.com)
The assay is often repeated multiple times on certain molecules. (physicsforums.com)
In the biological sciences the suffix -omics is used to refer to the study of large sets of biological molecules. (who.int)
Through various omic technologies it is now possible to interrogate large sets of biological molecules. (who.int)

Multiple metals are analyzed but the majority of the analyses is for arsenic and its chemical species (e.g. metabolites). (nih.gov)
The eluate was concentrated, and the phthalate metabolites chromatographically resolved by reversed-phase HPLC, detected by atmospheric pressure chemical ionization-tandem mass spectrometry (APCI-MS/MS), and quantified by isotope dilution. (cdc.gov)

It is a first of its kind in Canada hosting pioneering research on quantitative understanding of persistent and toxic organic pollution and their effects in harsh marine and freshwater environments and development of innovative engineering and managerial solutions by integrating environmental engineering with nano/biological technologies and risk-simulation-optimization methods. (mun.ca)
All the information obtained is thus used to enhance existing or developing methods to assess the joint toxic action of environmental chemicals. (cdc.gov)
Human beings and environmental species are exposed to tens of thousands of chemicals, only a small percentage of which have been tested thoroughly using standard in vivo test methods. (unl.edu)
or use data science methods and tools to deduce information about biological systems. (nih.gov)
Of interest are development of computational and mathematical algorithms and tools, modeling techniques and approaches for understanding the complexity of biological systems, and utilization of big datasets and data science methods for model construction. (nih.gov)
Rapid methods of measuring the effects of an agent in a biological or chemical assay. (bvsalud.org)

Independent of how different your specific assays themselves are, the underlying principle and general steps to transfer the assay on chip usually have similar requirements and are part of microfluidic ChipShop's daily business. (microfluidic-chipshop.com)

Evaluates the chemical and physical properties of various organic and inorganic substances. (salary.com)

Point-detection systems may be specific or non-specific, depending on their ability to discriminate a definite biological agent once the analysis is performed. (conceptcontrols.com)
Non-specific detection systems are only able to determine if a biological agent is present, without providing any identification. (conceptcontrols.com)
On the other hand, specific point-detection systems are able to return an identification of the biological agent. (conceptcontrols.com)

Early identification of an intentional biological event is essential to ensure correct management and response to the emergency. (conceptcontrols.com)
The Hazard Identification Core efficiently provides common analyses (biological or metal analyses) to its SBRP investigators thus allowing for more SBRP funds to be directed to the individual research projects as well as uniform quality control of the analyses. (nih.gov)
Chemical allergens encountered in choice for the hazard identification of skin-sensi- the skin are recognized by Langerhans' cells tizing chemicals. (cdc.gov)
rate identification of skin-sensitization hazard, LLNA protocol and chemicals tested. (cdc.gov)

Science and Technology Corporation (STC) is working with our customers on a wide variety of projects that focus on basic and applied R&D, the design of chemical-biological defense systems, and the safest ways to demilitarize and dispose of chemical warfare agents. (stcnet.com)

Understanding the hazards related to skin contact with chemicals is a critical component of modern occupational safety and health programs. (cdc.gov)

Exposure to a potential fetal health hazard can be estimated through ecological assessment (e.g., testing the community water supply), questionnaires (e.g., classifying residents accord- ing to whether they drink bottled or tap water), or biological markers (e.g., testing for exposures to chemicals or solvents found in tap water). (cdc.gov)
If omics-based assays were applied with appropriate study designs, thorough validation of the markers, and careful interpretation of study results then a bioinformatics database could be built of the human response to different chemical exposures and associated chronic diseases. (who.int)
This database could be useful in many ways for evaluating the risk posed by chemical exposures. (who.int)

The Metal Analyses section uses ICP-Mass Spectrometer analysis for total metal content and HPLC-ICP-MS for analysis of chemical species of metal. (nih.gov)
and non-free radical species such as hydrogen peroxide (H 2 O 2 ) are diverse kinds of activated oxygen and mostly produced by exogenous factors or oxidation product of biological reactions [ 1 - 3 ]. (ijpsonline.com)

Over a number of years, ATSDR scientists in collaboration with mixtures risk assessors and laboratory scientists have developed approaches for the assessment of the joint toxic action of chemical mixtures. (cdc.gov)
The purpose of an Interaction Profile is to evaluate data on the toxicology of the "whole" priority mixture (if available) and on the joint toxic action of the chemicals in the mixture in order to recommend approaches for the exposure-based assessment of the potential hazard to public health. (cdc.gov)

ATSDR will select substances/chemicals for development of interaction profiles through inter/intra agency communications and literature reviews. (cdc.gov)
PAHs constitute a group of chemicals, which are formed during the incomplete combustion of coal, oil and gas, garbage, and other organic substances. (cdc.gov)
Chemical Reference Substances (now totalling 213) and International Infrared Reference Spectra (now totalling 69). (who.int)

Relationship between pyrogenicity and bacterial endotoxin contamination on latex products was demonstrated by chemical analysis and biological assays. (nih.gov)
In support of the Program and Integration Support contract, STC provides environmental science, air monitoring and analysis and project scheduling support to the Chemical Materials Activity for the destruction of the stockpile of lethal unitary chemical agents and munitions and chemical warfare related materials. (stcnet.com)
The objectives of this Core are to provide research support in two areas: Biological Response and Metal Analysis. (nih.gov)
Biostatistics areas of interest include development of advanced statistical techniques and methodologies for design of biological experiments, collection and analysis of the data from those experiments and interpretation of, and inference from, the results. (nih.gov)

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. (sigmaaldrich.com)

This Catalyze Product Definition Funding Opportunity Announcement (FOA) will provide the early stage translational support needed to develop and test device prototype designs, identify diagnostic disease targets and develop associated assays, and develop research tools for use in the treatment of heart, lung, blood and sleep (HLBS) diseases and disorders. (nih.gov)
Expertise and capability in functional genomics and DNA based assay design can be applied to numerous disciplines, research interests and diagnostic test development in the food or animal testing areas. (dcu.ie)
With our lab-on-a-chip systems we aim to make biological and diagnostic assays simpler, more sensitive and less error prone. (microfluidic-chipshop.com)
The potential uses of tests for either ricin or ricinine in human biological samples would be primarily for purposes of confirming exposure or assessing the prevalence of exposure rather than diagnostic use. (cdc.gov)

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate. (sigmaaldrich.com)
Cuvette or 96-well plate assay formats possible. (immpact-international.org)

Scientists spend countless hours synthesizing and testing chemicals, only to discover they are environmentally hazardous late in development. (acdlabs.com)
To learn more about CCRP, see a June 2020 article, The National Institutes of Health Chemical Countermeasures Research Program (NIH CCRP): A collaborative opportunity to develop effective and accessible chemical medical countermeasures for the American people , published in the journal Drug Development Research. (nih.gov)
STC supports the U.S. Army Research, Development, and Engineering Command's Edgewood Chemical Biological Center (ECBC) with CB and obscurant R&D. (stcnet.com)
NICEATM and NC3Rs are partnering with the National Institute of Allergy and Infectious Diseases, the U.S. Army Combat Capabilities Development Command Chemical Biological Center, and NCATS to direct the MPS for COVID Research (MPSCoRe) working group. (nih.gov)
Cell-based assay development for drug discovery can accelerate optimization of treatments for cancer and other angiogenesis-dependent diseases. (sigmaaldrich.com)
Thus, this study demonstrated for the first time that the glycolic extracts of C. pachystachya have promising chemical and biological characteristics for the development of a multifunctional cosmetic with antioxidant and tyrosinase-inhibition activities. (bvsalud.org)

For this reason, the skin is often exposed to potentially hazardous agents, including chemicals, which may contribute to the onset of a spectrum of adverse health effects ranging from localized damage (e.g., irritant contact dermatitis and corrosion) to induction of immune-mediated responses (e.g., allergic contact dermatitis and pulmonary responses), or systemic toxicity (e.g., neurotoxicity and hepatoxicity). (cdc.gov)

We did so by considering that small chemicals have potent biological properties, that single atom changes in their structure can affect those properties, that chemical structure can be modulated, and that as such they constitute highly refined biological probes. (nature.com)
Case study and paper discussion Design, Synthesis of Novel, Potent, Selective, Orally Bio-available Adenosine A2A Receptor Antagonists and Their Biological Evaluation J. Med. (brighttalk.com)

One of these compounds (ebselen) also exhibited promising antiviral activity in cell-based assays. (nature.com)
By coupling efficient chemical synthesis routes to multiple upfront in parallel phenotypic screens, we identify that KBU2046 inhibits cell motility and cell invasion in vitro. (nature.com)
The advantages of cell-based assays include the facilitation and generation of complex and biologically relevant data. (sigmaaldrich.com)
Explore tumor metastasis employing various cell migration and invasion assays like Boyden chamber assay, Millicell® µ-Migration Assay and in vitro scratch assay. (sigmaaldrich.com)
Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology. (sigmaaldrich.com)
Learn how to set up a T cell killing assay using Millicell ® Microwell plates. (sigmaaldrich.com)
These assays may be used to quantify and to assess the health of mammalian cell lines, primary cells and stem cells. (sigmaaldrich.com)
Cell migration is the movement of cells in response to specific external signals, including chemical and mechanical stimuli. (sigmaaldrich.com)
Cell migration and invasion can be evaluated in vitro with widely-used membrane-based migration systems such as the Boyden Chamber assay. (sigmaaldrich.com)
Angiogenesis assays can be used to study both the induction and inhibition of angiogenesis in in vitro cell models. (sigmaaldrich.com)

Several case studies, and the employment of more sensitive biological and physical measuring techniques have, however, gone a long way to dispel any doubts about the presence and negative physiological consequences of EDC's in water sources used for domestic as well as recreational purposes. (sun.ac.za)

Previous studies investigating GWI have suggested that exposure to organophosphates (OP) in theater, such as the irreversible acetylcholinesterase (AChE) inhibitor and chemical warfare agent, sarin, as well as other pesticides , may have contributed to GWI symptomatology. (cdc.gov)
and (vii) developing exposure models to identify chemicals for which these hazardous dose levels could be achieved. (unl.edu)
In this example, a biological exposure marker (blood lead-level) was available for classifying mothers according to their exposure to lead. (cdc.gov)
Following topical sensitization, epider- draining lymph nodes following topical exposure mal LCs transport antigen from the skin to drain- of mice to chemicals (3-7). (cdc.gov)
Biomeasures of phthalates in humans are necessary to evaluate potential human health threats from exposure to these chemicals. (cdc.gov)

Today's approval exemplifies the FDA's longstanding commitment to support a competitive marketplace for biological products," Sarah Yim, MD, director of the Office of Therapeutic Biologics and Biosimilars in the FDA's Center for Drug Evaluation and Research, said in a statement . (medscape.com)
Because humans are the primary subject of interest for regulatory safety testing, it is advantageous to have human reference data available for evaluation of NAMs for assessing chemical safety. (nih.gov)

This study provides a novel complete toolbox of CB2 functional modulators that derive from the same chemical scaffold. (nih.gov)

However, with existing bioluminescent reporter technology, this is simply not possible because the current market of bioluminescent reporter cells being applied toward toxicology screening relies upon a firefly luciferase gene construct that must be provided with a chemical substrate to activate its light emission response, resulting in only marginally informative single time point snapshots of potential toxicological interactions. (inknowvation.com)

Herein, we delineate a novel and selective regulatory mechanism for these processes using efficient synthesis routes and resultant small chemicals as biological probes. (nature.com)

Before the 20th century, biological warfare took three main forms: (1) deliberate poisoning of food and water with infectious or toxic material, (2) use of microorganisms or toxins in some form of weapon system, and (3) use of biologically inoculated fabrics. (medscape.com)

His aim was to develop a chemical method for measuring the oestrogens in the urine and was given a position in the newly established Clinical Endocrinology Research Unit in the University of Edinburgh, later to be appointed its Assistant Director. (wikipedia.org)

The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays. (bvsalud.org)

The materials that comprise this dataset encompass both the chemical and biological diversity of known chemical allergens and provide also examples of negative controls. (cdc.gov)

Accurate chemical nomenclature and reporting are essential components of environmental safety. (acdlabs.com)
Generate chemical names according to IUPAC rules, convert chemical names to structures, and handle challenging areas of nomenclature. (acdlabs.com)

Low interference in biological samples. (immpact-international.org)

The German-American physician Anton Dilger established a secret biological laboratory in Chevy Chase, Maryland, with the intent to grow the causative agents of anthrax and glanders. (medscape.com)
There are no specific clinically validated assays for detection of ricin that can be performed by the hospital/healthcare facility clinical laboratory. (cdc.gov)

This document provides the scientific rationale and framework for the assignment of multiple hazard-specific skin notations (SK) that clearly distinguish between the systemic effects, direct (localized) effects, and immune-mediated responses caused by skin contact with chemicals. (cdc.gov)
NAMs for chemical hazard assessment are often evaluated in comparison to animal studies. (nih.gov)

To cope with the complex task of developing such systems, we adapt standard assay steps to the special requirements of the microfluidic surrounding. (microfluidic-chipshop.com)
The potential spectrum of bioterrorism ranges from hoaxes and actual use of agents by individuals or groups against others, to state-sponsored terrorism that employs biological warfare (BW) agents and delivery systems that can produce mass casualties. (medscape.com)

Furthermore, by leveraging human cells as the sensing platform, our assay provides more accurate and realistic information in regards to bioavailability and a chemical's true effect on individual human health than does the employment of small animal models. (inknowvation.com)
establish mechanisms of action by studying the effects of the same chemicals in experimental animals and on human cells in vitro, allowing for a better prediction of human carcinogenicity and assessment of carcinogenic mechanisms. (who.int)
During World War II, the Japanese operated a secret biological warfare research facility in Manchuria and carried out human experiments on Chinese prisoners. (medscape.com)

chemical derivatization of natural products [Note that throughout this RFA, the term "natural product" refers to a single, well-defined chemical compound, as opposed to a mixture or extract, obtained from a living organism. (nih.gov)
Majority of the synthesized compounds get filtered out in this step, hence its extremely important to set up the assays in such away that the outcome provide true reliable measure of effect of each compound. (brighttalk.com)

Biohazards represent an important issue in the field of security, both for the destructive potential and the psychological, economic and social impact that the use of biological agents for biowarfare could have on populations. (conceptcontrols.com)
The use of biological agents is not a new concept, and history is replete with examples of biological weapons use. (medscape.com)
This was the first multilateral agreement that extended prohibition of chemical agents to biological agents. (medscape.com)

To cope with these tasks, microfluidic ChipShop's biological and chemical laboratories are well equipped and experienced application teams are at hand. (microfluidic-chipshop.com)

In meeting this objective, this Skin Notation Profile intends to inform the audience--mostly occupational health practitioners, researchers, policy- and decision- makers, employers, and workers in potentially hazardous workplaces--so that improved risk- management practices may be developed to better protect workers from the risks of skin contact with the chemicals of interest. (cdc.gov)

Chemical additions to creosote were screened using this method. (irg-wp.com)
Application of this method to the National Health and Nutrition Examination Survey (NHANES) project will help determine the concentration range of these chemicals in residents in the United States. (cdc.gov)

Chemicals and Drugs2

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes1

Information Science1

ELISA3

Biochemical assays1

High throughput assay3

Vitro assay1

Cytotoxicity4

Viability2

Characterization2

Cellular4

Standard deviation2

Efficacy1

Nucleic acids1

Inhibitors2

Molecules5

Metabolites2

Methods6

Principle1

Evaluates1

Detection systems3

Identification4

Warfare agents1

Critical1

Exposures3

Species2

Approaches2

Substances3

Analysis4

Protocol1

Diagnostic4

Plate2

Development6

Toxicity1

Potent2

Cell10

Techniques1

Exposure5

Evaluation2

Functional1

Interactions1

Probes1

Biologically1

Urine1

Involves1

Materials1

Nomenclature2

Samples1

Laboratory2

Hazard2

Systems2

Human3

Compound2

Agents3

Tasks1

Hazardous1

Method2