Biofilms. Medical search. Frequent questions (2)

Owing to its ability to form biofilms on medical devices, including indwelling catheters, artificial heart valves, and endotracheal tubes, S. aureus often causes chronic or fatal infections in clinical settings. (nature.com)
A relatively new threat, the Candida auris fungus has become a dangerous agent due to its ability to form biofilms, which help it survive and spread infections within the environment. (infectioncontroltoday.com)
The ability to form biofilms is a key factor in the persistence of tested strains in the environment. (nih.gov)
It has been recently demonstrated that App has the ability to form biofilms in vitro. (umontreal.ca)

RNA purified from the medium promoted biofilm formation. (nature.com)

Here, we show that RNA contributes to the structural integrity of biofilms formed by the human pathogen Staphylococcus aureus . (nature.com)
The goal of this study was to investigate the effect of the environmental conditions such as the temperature change, incubation time and surface type on the resistance of Staphylococcus aureus biofilms to disinfectants. (researchgate.net)
Staphylococcus aureus biofilms to disinfectants. (researchgate.net)
The present study aims to establish the relationship between biofilm formation and antibiotic resistance and adhesion genes in Staphylococcus aureus strains isolated from raw cow milk in Shahrekord, Iran. (hindawi.com)

A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
Biofilms are diverse communities of microorganisms embedded within a self-produced matrix of extracellular polymeric substance which are firmly attached to biotic or abiotic surfaces. (medscape.com)
[ 4 ] The formation and behavior of the entire biofilm community is directed by signaling molecules that are produced when microorganisms reach a critical number. (medscape.com)
RESEARCH OBJECTIVES Background A biofilm is an accumulation of microorganisms (bacteria, fungi, and/or protozoa, with associated bacteriophages and other viruses) embedded in a polysaccharide matrix and adherent to solid biologic or non-biologic surface. (nih.gov)
Additionally, what other microorganisms are able to survive in a biofilm? (infectioncontroltoday.com)
A biofilm is an architectural colony of microorganisms, that is irreversibly associated (not removed by gentle rinsing) with a surface and enclosed in a matrix of primarily polysaccharide material. (physio-pedia.com)
Biofilms are the most common form of life of many microorganisms and can occur in various natural or technical environments. (zhaw.ch)
The findings indicate biofilms occur in water lines when taps are not turned on for extended periods of time, allowing stagnant water to accumulate a glue-like mixture of bacteria, fungi, amoebas and other potentially harmful microorganisms. (aboutlawsuits.com)
While biofilms have shown to support the persistence of microorganisms, the degree by which this matrix is truly pathogenic remains unknown given its high prevalence even in asymptomatic patients. (medscape.com)
Algae, protozoa, and fungi may be present in biofilms, but the predominant microorganisms of water system biofilms are gram-negative bacteria. (cdc.gov)

While bacteria have classically been viewed from the perspective of planktonic, free floating pathogens proliferating and exerting their virulence as individual organisms, it is now recognized that microbes also can exist as multicellular consortiums known as biofilms. (medscape.com)
But researchers found that when mycobacteria closely related to the TB and leprosy pathogens lack one key protein, mature biofilms fail to form. (medgadget.com)
This study assessed the anti-biofilm activity of paeonol against Gram-negative pathogens and investigated the effect of paeonol on QS-regulated virulence factors in. (greenmedinfo.com)
Moreover, different pathogens often colonize the same biofilm and exchange antibiotic-resistant genes, making infections more difficult to treat. (infectioncontroltoday.com)
Our screen allowed the identification of genes already associated in biofilm formation of App (hns) or other pathogens (potD2, ptsI, tig and rpmF). (umontreal.ca)
In a study published this week in the U.S. Centers for Disease Control and Prevention (CDC)'s Emerging Infectious Disease journal, researchers warn that chlorine-tolerant biofilm-related pathogens are building up in U.S. water supply lines, exposing millions to more than 17 different waterborne infectious diseases, and adding billions in financial strain on the nation's healthcare system. (aboutlawsuits.com)
The waterborne diseases most frequently resulting in hospitalizations and fatalities were biofilm pathogens such as Nontuberculous mycobacteria disease, Legionella bacteria and Pseudomonas pneumonia. (aboutlawsuits.com)
The data presented here reflect the changing picture of waterborne disease in the United States and underscore the role of environmental pathogens that grow in biofilms," the researchers warned. (aboutlawsuits.com)

These findings provide evidence of a function for RNA that can lead to the development of strategies to prevent biofilm formation. (nature.com)
Using smoother surfaces may also prevent biofilm formation and we showed that the clinical lifetime was increased by a factor 2.1. (rug.nl)
This stimulated research is a rapidly growing field for applicable control measures to prevent biofilm formation. (frontiersin.org)

Approximately 80% of all human infections are associated with biofilms and evidence for their role in an ever-growing number of cutaneous disorders is constantly unfolding. (medscape.com)
Biofilms have been associated with approximately 80% of all human infections, yet their detection is extremely difficult with the use of routine culture techniques. (medscape.com)
Herein, we will present current knowledge regarding the role of biofilms in cutaneous disease along with potential therapeutic strategies for the management of biofilmassociated infections. (medscape.com)
Infections that involve biofilms are challenging to treat. (nih.gov)
Biofilms are medically important, accounting for over 80 percent of microbial infections in the body. (nih.gov)
Biofilms are surface-bound microbial communities that are typically embedded in a matrix of self-produced extracellular polymeric substances and can cause chronic infections. (nature.com)
By curtailing the effects of antibiotics, communities of bacterial cells known as biofilms can be deadly during surgeries and in urinary tract infections. (yale.edu)
In American hospitals alone, thousands of deaths are attributed to biofilm-related surgical site infections and urinary tract infections. (yale.edu)
Biofilms are a huge medical problem because they are something that makes bacterial infections very difficult to deal with," said Andre Levchenko, senior author of the study, which was published Oct. 5 in Nature Communications . (yale.edu)
The formation of bacterial biofilm on implanted devices or damaged tissues leads to biomaterial-associated infections often resulting in life-threatening diseases and implant failure. (mdpi.com)
According to the NIH, 65% - 80% of chronic (recurring) infections are biofilm-related. (infectioncontroltoday.com)
Bacterial biofilms are usually pathogenic in nature and can cause hospital infections. (physio-pedia.com)
The National Institutes of Health (NIH) revealed that among all microbial and chronic infections, 65% and 80%, respectively, are associated with biofilm formation [2] . (physio-pedia.com)
Microbial biofilms cause problems in the hospital environment, generating high healthcare costs and prolonged patient stay, also at times resulting in further secondary microbial infections and various health complications. (physio-pedia.com)
It is estimated that about 65% of all bacterial infections are associated with bacterial biofilms. (physio-pedia.com)
Biofilms causing infections are progressive, and in some cases can become a chronic problem. (physio-pedia.com)
Bacterial biofilms are the major cause of chronic wound infections and are notoriously recalcitrant to treatments with antibiotics, making them difficult to eradicate. (frontiersin.org)
In addition, manuka honey exhibits anti-biofilm activity, thereby warranting the investigation of its potential as a combination therapy with antibiotics for the topical treatment of biofilm-related infections. (frontiersin.org)
Here we report the first use of MacSynergy II to investigate the response of established S. aureus (strain NCTC 8325) biofilms to treatment by combinations of Medihoney (medical grade manuka honey) and conventional antibiotics that are used for preventing or treating infections: rifampicin, oxacillin, fusidic acid, clindamycin, and gentamicin. (frontiersin.org)
The development of intact biofilms is very critical for the spreading and persistence of bacterial infections in the host. (frontiersin.org)
Further, bacteria form biofilms on every probable substratum, and their infections have been found in plants, livestock, and humans. (frontiersin.org)
Therefore, this review discusses the current understanding of antibiotic resistance mechanisms in bacterial biofilm and intensely emphasized the novel therapeutic strategies for combating biofilm mediated infections. (frontiersin.org)
Although the association of biofilms and medical devices provides a plausible explanation for a variety of health-care associated infections, it is not clear how the presence of biofilms in the water system may influence the rates of health-care- associated waterborne infection. (cdc.gov)
Bakaletz' research team is exploring the structure of biofilms in chinchillas, the gold standard animal model for studying ear infections. (nih.gov)
Interestingly, this research could also help battle many other types of infections involving biofilms. (nih.gov)

To demonstrate the role of biofilms in immune response, the researchers wanted to see how the sentinels of the immune system, called dendritic cells, reacted to a biofilm. (scienceblog.com)
several of the contributions in this book specifically focus on the role of biofilms in disease processes (Chapters 6, 7, 8, 14, and 19) or biofilm susceptibility to antimicrobial agents (Chapters 2 and 13). (cdc.gov)
The emphasis on the role of biofilms in the pathogenesis of various microbial diseases, as well as discussions of biofilm tolerance and antimicrobial resistance should also be helpful and interesting to anyone working in the field of healthcare delivery. (cdc.gov)

Bacterial communities known as biofilms may play a role in the development of systemic lupus erythematosus (SLE), according to a study conducted in mice and funded in part by the NIH's National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS). (nih.gov)

Additional experiments revealed that some of the genes previously shown to be required for biofilm formation on smooth surfaces were not needed to form biofilm streamers in the flow system used in this study, underscoring the need to study biofilms in realistic environments. (nih.gov)
A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated. (wikipedia.org)
However, as a result of advances in laser technology, digital imaging, scanning electron microscopy, and new fluorescent probes, researchers can now build a three dimensional model of biofilms and identify the location in the biofilm where specific genes are being expressed. (nih.gov)
Now we have discovered that adhesion triggers the expression of a sigma factor that derepresses a large number of genes so that biofilm cells are clearly phenotypically distinct from their planktonic counterparts. (nih.gov)
Biofilm-associated organisms also differ from their planktonic (freely suspended) counterparts with respect to the genes that are transcribed. (physio-pedia.com)
PCR was used to detect several biofilm and antibiotics resistance related genes. (hindawi.com)
Detection of biofilm-related genes shows that all the genes targeted were found among S. aureus isolates. (hindawi.com)
The objective of this work is to identifiy genes implicated in the biosynthesis and regulation of biofilm formation in App. (umontreal.ca)
Analysis revealed novel genes implicated in biosynthesis and regulation of the biofilm formation in App. (umontreal.ca)
Genes (APL_0049, APL_0637 and APL_1573) that have not yet been associated with biofilm formation were also identified. (umontreal.ca)
Further characterization of the genes mentioned above would permit a greater understanding of the mechanisms implicated in biofilm formation of App. (umontreal.ca)

Microbes form a biofilm in response to a number of different factors, which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics. (wikipedia.org)
The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
Very little was known about how biofilms interact with the immune system because most of the research has been looking at how biofilms protect bacteria, how they make bacteria resistant to antimicrobials such as antibiotics, but almost nothing was known about what biofilms do to the immune response," she said. (scienceblog.com)
For S. aureus and P. aeruginosa dual species biofilms, it has been numerously reported that P. aeruginosa decreases S. aureus susceptibility to a broad range of antibiotics, including beta-lactams, glycopeptides, aminoglycosides, macrolides, while sensitizes to quinolones via secretion of various metabolites. (nih.gov)
Moreover, similar increase in antibiotics efficacy could be observed following addition of S. aureus suspension to the P. aeruginosa mature biofilm, compared to P. aeruginosa monoculture, and vice versa. (nih.gov)
The inability of traditional antibiotics to penetrate oral biofilms at therapeutic concentrations is a major reason for their lack of efficacy in treating chronic periodontitis. (businesswire.com)
Biofilms are one of the primary reasons that other molecules like broad spectrum antibiotics are ineffective against P. gingivalis . (businesswire.com)
Adversely, the large doses of antibiotics used to treat biofilms clinically have also contributed to the development of antibiotic-resistant bacteria strains. (physio-pedia.com)
Using checkerboard microdilution assays, viability assays and MacSynergy II analysis we show that the Medihoney-rifampicin combination was more effective than combinations using the other antibiotics against established staphylococcal biofilms. (frontiersin.org)
Other combinations of Medihoney and antibiotic produced an interesting array of effects: Medihoney-fusidic acid treatment showed minor synergistic activity, and Medihoney-clindamycin, -gentamicin, and -oxacillin combinations showed overall antagonistic effects when the honey was used at sub-inhibitory concentration, due to enhanced biofilm formation at these concentrations which could not be counteracted by the antibiotics. (frontiersin.org)
Biofilm formation defends bacteria against desiccation, the host's immune defences, and the action of oxidising biocides and antibiotics [ 18 ]. (hindawi.com)
Bacterial biofilms are communities of adherent bacteria protected against the body's immune system and antibiotics by a protein-containing polysaccharide matrix. (medscape.com)
Research has shown that biofilm-associated organisms are more resistant to antibiotics and disinfectants than are planktonic organisms, either because the cells are protected by the polymer matrix, or because they are physiologically different. (cdc.gov)
This biofilm houses the bacteria and shields it from your immune system and from antibiotics. (nih.gov)

Organisms within biofilms are embedded in a glycocalyx, a self-produced matrix of extracellular polymeric substance (EPS) composed of polysaccharides, proteins, lipids, and extracellular DNA (eDNA). (medscape.com)
[ 6 ] New methods to detect biofilm-associated organisms are under development. (medscape.com)
Conventional therapy is characteristically ineffective against biofilms, as the minimum inhibitory concentration (MIC) of antimicrobial agents has been shown to be 10 to 1000 fold greater than for planktonic organisms. (medscape.com)
[ 10 ] Antimicrobial resistance can be attributed to the EPS serving as a physical barrier to antibiotic penetration, plasmid exchange facilitated by close proximity between organisms, and the low metabolic activity and growth rate observed within biofilms. (medscape.com)
I found particularly helpful the use of tolerance factors to compare reduced susceptibility of different biofilm-associated organisms toward biocides, antiseptics, and antibiotic drugs. (cdc.gov)
Tolerance factors were plotted as a function of antimicrobial agent molecular weight, substratum material, and biofilm density, providing the reader a method for quickly visualizing these patterns for a wide range of organisms. (cdc.gov)
Organisms living in biofilms can be up to 1,000-fold more antibiotic resistant and 1,500 times less susceptible to disinfectants. (infectioncontroltoday.com)
Educating staff to raise awareness of the dangers of surface biofilms, the organisms they harbor, and the necessity for effective cleaning and disinfection to eradicate them. (infectioncontroltoday.com)
Describes the types of organisms often present in drinking water distribution system biofilms, how biofilms are established & grow, the public health problems associated with having biofilms in the distribution system, & tools that water treatment personnel can use to help control biofilm growth. (dianepublishing.net)
These adherent organisms can initiate and develop biofilms, which are comprised of cells embedded in a matrix of extracellularly produced polymers and associated abiotic particles. (cdc.gov)
Although most of these organisms will not normally pose a problem for healthy individuals, certain biofilm bacteria (e.g. (cdc.gov)
1441, 1442 These biofilm organisms may easily contaminate indwelling medical devices or intravenous (IV) fluids, and they could be transferred on the hands of health-care workers. (cdc.gov)
Biofilms may potentially provide an environment for the survival of pathogenic organisms, such as Legionella pneumophila and E. coli O157:H7. (cdc.gov)
Organisms within biofilms behave quite differently than their planktonic (i.e., free floating) counterparts. (cdc.gov)
If an epidemiologic investigation points to the water supply system as a possible source of infection, then water sampling for biofilm organisms should be considered so that prevention and control strategies can be developed. (cdc.gov)

Biofilms are communities made up of many individual bacterial cells held together and stuck to surfaces by a kind of biological glue. (nih.gov)
Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
[ 1 , 2 ] (Figure 1) Several advantages exist for bacteria that live in a biofilm phenotype including structural stability, firm adherence to biotic or abiotic surfaces, increased virulence, and resistance to both antimicrobial therapy and the host immune response. (medscape.com)
Researchers developed magnetic nanoparticle robots that destroy and remove biofilms from surfaces. (nih.gov)
A biofilm is a layer of microbes, usually bacteria, that grows on many kinds of surfaces, including teeth and medical devices. (nih.gov)
Microbial biofilms are present on biotic and abiotic surfaces and have a significant impact on many fields in industry, health care and technology. (spie.org)
Nearly microbes of every species have mechanisms by which they can adhere to biotic or abiotic surfaces and to each other, allowing them to exist in biofilms 3 . (nature.com)
Biofilms can be found almost everywhere, including unwashed shower stalls or the surfaces of lakes. (yale.edu)
The list of microbes, including gram-positive/gram-negative bacteria and fungi, that can cling to surfaces and medical devices in biofilms is long and diverse. (infectioncontroltoday.com)
The list of surfaces where biofilms persist is also long and diverse. (infectioncontroltoday.com)
Biofilm reservoirs enable C auris to persist on surfaces and devices longer-from weeks to months-which can subsequently lead to outbreaks in health care settings. (infectioncontroltoday.com)
Studies have shown that a single touch can transfer biofilm bacteria and fungi to a health care worker's hands, even with gloves, which can then be transferred to patients and contaminate multiple other surfaces. (infectioncontroltoday.com)
Targeted protocols focused on cleaning and disinfecting high-risk surfaces most prone to biofilm formation, including reusable and shared equipment. (infectioncontroltoday.com)
Biofilms may form on various surfaces, like living tissues, indwelling medical devices, industrial or potable water system piping, or natural aquatic systems. (physio-pedia.com)
Biofilms: microbial life on surfaces. (physio-pedia.com)
The non-oxidizing biocide ethyl lauroyl arginate (LAE) is promising for mitigating biofilm development on membrane surfaces. (mdpi.com)
Bacterial biofilms can attach to various materials such as metals, glass surfaces, plastic wares, tissues, and clinical devices. (frontiersin.org)
Bacteria like NTHI often create a coating - called a biofilm - on the surfaces of your middle ear. (nih.gov)

Hyperthermophilic archaeon Pyrobaculum calidifontis produce bundling pili which are homologous to the bacterial TasA filaments, a major component of the extracellular matrix in bacterial biofilms, which contribute to biofilm stability. (wikipedia.org)
Identifying biofilm matrix components that contribute to biofilm structural integrity can provide insight into the process of biofilm development that can lead to the development of strategies for the inhibition of biofilm formation. (nature.com)

In addition, bacteria within biofilms have increased (up to 1000-fold higher) resistance to antimicrobial compounds, even though these same bacteria are sensitive to these agents if grown under planktonic conditions. (nih.gov)
Also it has been seen that some bacteria within biofilms, called "persister cells," are dormant variants that exhibit antibiotic tolerance and can become active when the therapy is withdrawn. (physio-pedia.com)

At Cortexyme's IADR 2021 poster session titled "Gingipain Inhibitors Penetrate And Inhibit Gingipains In Porphyromonas gingivalis Biofilms" (Abstract #3571509) taking place Friday, July 23, 2021, starting at 3:45 p.m. (businesswire.com)

Chapter 1 provides a balanced comparison of static and continuous flow methods for growing biofilms that should be beneficial for researchers investigating biofilm development or dispersion, and for applied studies evaluating new treatment strategies for biofilm prevention and control. (cdc.gov)

[1] Biofilm contains microbial cells adherent to one-another and to a static surface (living or non-living) [2] . (physio-pedia.com)
1 There is no longer a need to flush DUWLs for 1 minute to 2 minutes at the beginning of the day, as evidence suggests that firmly adherent biofilms are not dislodged by flushing alone. (dimensionsofdentalhygiene.com)

Existing treatments for biofilms are ineffective because they are incapable of simultaneously degrading the protective matrix, killing the embedded bacteria, and physically removing the biodegraded products," Koo says. (nih.gov)
Because the protective shell can keep out potential treatments, biofilms are at their most dangerous when they invade human cells or form on sutures and catheters used in surgeries. (yale.edu)
It is a challenging process to eradicate biofilms as they are resistant to antimicrobial treatments. (mdpi.com)
Due to the widespread distribution of biofilms in diseases and their resilience to numerous antimicrobial treatments, biofilm research is receiving more attention. (physio-pedia.com)
Confocal scanning laser microscopy confirmed that different honey-antibiotic combination treatments could eradicate biofilms. (frontiersin.org)
The forthcoming experimental studies will focus on these recent therapeutic strategies that may lead to the development of effective biofilm inhibitors than conventional treatments. (frontiersin.org)
Once DUWL quality is determined, initial, periodic, or continuous chemical "shock" treatments are recommended to enhance the performance of products and to reduce or eliminate biofilm. (dimensionsofdentalhygiene.com)

Paeonol exhibited an effective anti-biofilm activity against Gram-negative bacteria. (greenmedinfo.com)

The resistance of S. aureus biofilm seems to depend on the environment of the biofilm formation and involves both extracellular matrix and membrane fluidity of sessile cells. (researchgate.net)
Biofilms and lichens alike have distinct features that allow for their lifestyle and identification, such as specific developmental patterns, formation of an extracellular matrix, and their ability to resist abiotic stressors. (unl.edu)
A common strategy of bacteria for adaptation and survival to changing environmental conditions is the formation of biofilms, which are bacterial communities enclosed in an extracellular matrix. (elifesciences.org)

Microbes adhere to a surface and create protective biofilms as a barrier against the external environment, including many antimicrobial agents. (infectioncontroltoday.com)

The field of biofilm research has traditionally been hindered by an inability to study the biofilm in non-destructive, three dimensional ways. (nih.gov)
Innovotech Inc. (TSX VENTURE:IOT), a pioneer in the field of biofilm product development, today announced that its MBEC Assay™ has been approved as an ASTM International standard. (dentistryiq.com)

The structure of biofilms is such that immune responses may be directed only at those antigens found on the outer surface of the biofilm, and antibodies and other serum or salivary proteins often fail to penetrate into the biofilm. (nih.gov)

Researchers at Princeton University used time-lapse microscopy imaging to monitor fluid flow and biofilm formation in curvy, narrow tubes. (nih.gov)
The researchers aimed to use these "catalytic antimicrobial robots" to break up biofilm matrix, kill the bacteria inside, and remove the debris. (nih.gov)
The researchers showed that the robotic system could scrub the sides of the tube, even when clogged with biofilm. (nih.gov)
Now, researchers at Temple University School of Medicine (TUSM) have shown that bacterial communities known as biofilm play a role in the development of the autoimmune disease systemic lupus erythematosus - a discovery that may provide important clues about several autoimmune ailments. (scienceblog.com)
A team led by TUSM researchers Çagla Tükel, PhD, and Stefania Gallucci, MD, show how bacterial biofilms found in the gut can provoke the onset of lupus in lupus-prone mice. (scienceblog.com)
Curli-DNA complexes speed up the creation of the biofilm, the researchers learned. (scienceblog.com)
HHMI researchers have identified a gene that enables mycobacteria-the cause of tuberculosis and leprosy-to form biofilms. (medgadget.com)
The target audience is biofilm researchers, but this second edition of the book should also be useful for healthcare practitioners seeking a better understanding of microbial biofilms in healthcare delivery. (cdc.gov)
In summary, Microbial Biofilms is a useful compendium suitable for students and a practical guide for researchers investigating new biofilm treatment strategies. (cdc.gov)
Yale researchers have just come a lot closer to understanding how these biofilms develop, and potentially how to stop them. (yale.edu)
However, the researchers in the Levchenko lab , working with colleagues at the University of California-San Diego, recently found a key mechanism for biofilm formation that also provides a way to study this process in a controlled and reproducible way. (yale.edu)
With this discovery, Levchenko said, researchers can use various devices that mimic other cellular environments and explore biofilm formation under countless environments and circumstances. (yale.edu)
In the current study, a team of researchers led by Çagla Tükel, Ph.D., and Stefania Gallucci, M.D., both of the Lewis Katz School of Medicine at Temple University, investigated the role of bacterial biofilms in triggering autoimmunity and lupus. (nih.gov)
To further explore curli's role in autoimmunity and lupus, the researchers exposed immune cells called dendritic cells to a biofilm of Salmonella Typhimurium , a curli-producing bacterium and a frequent culprit in cases of food poisoning. (nih.gov)
The researchers also discovered that curli binds tightly to bacterial DNA found in biofilms, forming a potent combination that signals the presence of a threat to the immune system. (nih.gov)
By aiming their attack at this target, the researchers both prevented biofilms from forming, and broke apart biofilms that were already there. (nih.gov)

Image of cells forming a biofilm, a bacterial cell community protected from environmental stress and a source of antibiotic resistance, in a new gel device developed in the Levchenko lab. (yale.edu)
Statistical tests show a significant correlation between biofilm production and antibiotic resistance in S. aureus . (hindawi.com)
This study revealed that there is a significant correlation between biofilm production and antibiotic resistance in S. aureus isolated from raw milk. (hindawi.com)

To identify the major components of biofilm matrices, we evaluated the susceptibility of S. aureus biofilms to various enzymes. (nature.com)
Our study represents the first report describing the impact of environmental conditions on the matrix production, sessile cells membrane fluidity and resistance of S. aureus biofilms to disinfectants. (researchgate.net)

Even though there is no direct evidence yet that M. tuberculosis forms biofilms, Hatfull and Jacobs say it is highly likely that the two GroEL1 proteins act in similar ways to change mycolic acid synthesis-a hypothesis they plan to test next. (medgadget.com)

Chapter 3 provides several animal models for the evaluation of fungal biofilms (primarily Candida spp. (cdc.gov)
We argue here that one can gain insight into the cellular processes and evolutionary origins of lichens, which are currently undetermined, by applying knowledge gleaned from studies on microbial biofilms, with a particular focus on fungal biofilms. (unl.edu)

RNase A dispersed both fresh and mature biofilms, indicating the importance of RNA at various stages. (nature.com)

Scientists used a new technique to visualize fluorescently labeled bacteria in intact biofilms scraped from the tongues of 21 healthy human volunteers. (nih.gov)

They can also use the devices introduced in this study to produce biofilms rapidly, precisely, and in high numbers in a simple, inexpensive, and reproducible way. (yale.edu)
As many pathogenic bacteria produce biofilms, there is growing interest in studying the correlation between biofilm production and antimicrobial susceptibility profile [ 20 ]. (hindawi.com)

Development of new theoretical models for interstitial flow and diffusion in cellular biological media (tissues, biofilms, etc) through a consistent combination of the spatial averaging method for the derivation of upscaled equations, with the particle-in-cell method for the calculation of macroscale parameters as functions of microscale system properties. (aiche.org)
Transport phenomena in cellular biological media (biofilms, tissues, etc. (aiche.org)

A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
Each biofilm bacterium lives in a customized microniche in a complex microbial community that has primitive homeostasis, a primitive circulatory system, and metabolic cooperativity, and each of these sessile cells reacts to its special environment so that it differs fundamentally from a planktonic cell of the same species. (nih.gov)

Here, we show that colony rugosity, a biofilm-associated phenotype, is regulated by temperature in V. cholerae strains that naturally lack the master biofilm transcriptional regulator HapR. (elifesciences.org)
We show that this phenotype is due to biofilm formation. (nih.gov)

Self-contained water alone will not eradicate biofilm formation, so controlling the quality via devices, products, or procedures is necessary. (dimensionsofdentalhygiene.com)

The dendritic cells sent "tendrils" into the biofilm and ate up part of it to signal other molecules. (scienceblog.com)
I was super excited when I saw how activated the dendritic cells were on the biofilm " Dr. Gallucci said. (scienceblog.com)
Dendritic cells (green), key sentinels of the immune system, infiltrating a Salmonella biofilm (red). (nih.gov)

The ASM Conference on Biofilms will provide a platform to discuss the latest research, covering topics that range from diagnosis and the study of clinically-relevant biofilms, through environmental biofilms, to emerging technologies and their application to biofilms. (fems-microbiology.org)
With the exception of a brief discussion in Chapter 1, very little information is provided on the benefits and drawbacks of culture-independent methods to characterize clinically relevant biofilm communities. (cdc.gov)

We identified bacterial fibronectin- and fibrinogen-binding proteins as important for aggregation in SF, suggesting an important role of fibrin-containing clots in the formation of bacterial biofilm-like aggregates during joint infection. (nih.gov)

Scanning electron micrograph demonstrating the presence of mixed species biofilm in a chronic wound. (medscape.com)
Biofilms in chronic wounds. (medscape.com)
Thus, new approaches are required to combat biofilms in chronic wounds. (frontiersin.org)
Our results suggest that honey has potential as an adjunct treatment with rifampicin for chronic wounds infected with staphylococcal biofilms. (frontiersin.org)

Give them a suitable surface, some water and nutrients, and bacteria will likely put down stakes and form biofilms. (nih.gov)
A recent study funded in part by the National Institutes of Health revealed how biofilms cause such clogs: The bacteria form streamers that tangle with each other and trap other passing bacteria, creating a full blockage in a surprisingly short period of time. (nih.gov)
As the bacterial cells flowed through the microscopic channel, they attached to the inner wall, where they began to multiply and form a biofilm (seen in green in the movie above). (nih.gov)
The studies also showed that biofilm streamers form in stents, water filters and porous materials similar to those used in wastewater treatment plants, suggesting that the streamers are the likely explanation for why biofilms block up these materials. (nih.gov)
Biofilms can form on the teeth of most animals as dental plaque, where they may cause tooth decay and gum disease. (wikipedia.org)
Hydrophobicity can also affect the ability of bacteria to form biofilms. (wikipedia.org)
The Temple team discovered that when curli amyloids and DNA meet, they form remarkably durable bonds in the biofilm. (scienceblog.com)
Jacobs' and Hatfull's current study began with the unexpected observation by Hatfull's postdoctoral fellow, Anil Ohja, that a virus-infected strain of Mycobacterium smegmatis could not form proper biofilms. (medgadget.com)
In streams and other habitats, biofilms Footnote 1 are primary sites for carbon and nutrient transformations and form the base of food webs, contributing to local and global ecosystem functioning [ 14 ]. (springer.com)
By using the right frequencies, bacteria and adhesive nutrient layers are removed at the same time, making it impossible for a new biofilm to form. (solarimpulse.com)
Biofilms form when bacterial cells gather and develop structures that bond them in a gooey substance. (yale.edu)
The present study was conducted to evaluate the capacity of Listeria monocytogenes (L.m), Listeria innocua (L.i), and Escherichia coli to form biofilms on polystyrene support under different parameters by performing crystal violet (CV) staining technique. (nih.gov)
All the strains tested, presented more or less adhesion power depending on the variation of the studied parameters as well as the ability to form multispecies biofilms. (nih.gov)
The ability to adhere and form biofilms differs from one serogroup to another within the same species. (nih.gov)
Our study showed that L.m, L.i, and E. coli could adhere to polystyrene and form biofilms under different conditions. (nih.gov)
In patients, biofilms that form are resistant to the host's endogenous defences. (physio-pedia.com)
Apart from the production of enterotoxin, the ability of S. aureus to form a biofilm is essential for its long existence in a harsh environment [ 12 , 13 ]. (hindawi.com)
Flor strains of Saccharomyces cerevisiae form a biofilm on the surface of wine at the end of fermentation, when sugar is depleted and growth on ethanol becomes dependent on oxygen. (oregonstate.edu)
1438 It is inevitable that biofilms will form in most water systems. (cdc.gov)
1439 Biofilms may form under stagnant as well as flowing conditions, so storage tanks, in addition to water system piping, may be vulnerable to the development of biofilm, especially if water temperatures are low enough to allow the growth of thermophilic bacteria (e.g. (cdc.gov)

The C. albicans morphogenesis is regulated (inhibition of hyphae) and is important in biofilm formation. (rug.nl)
Atuzaginstat and the company's second generation COR588 exhibited significant time and concentration-dependent inhibition of lysine-gingipain activity, while maintaining their target selectivity within the biofilms to inhibit lysine-gingipain. (businesswire.com)

Bacteria embedded within biofilms are resistant to both immunological and non- specific defense mechanisms of the body. (nih.gov)
We first noted that biofilm cells are at least 500 times more resistant to antibacterial agents. (nih.gov)
The cells produced the biofilm components and suddenly became very antibiotic-resistant. (yale.edu)

The microbial composition of voice prosthetic biofilms was explored using molecular techniques. (rug.nl)
Because the prevention of biofilms is very important, we also evaluated voice prosthetic biofilm formation on antimicrobially-modified and smoothened silicone rubber voice prostheses. (rug.nl)

A list of commercial testing products and services cleared by the US Food and Drug Administration (FDA) is available at: ada.org/en/member-center/oral-health-topics/dental-unit-waterlines. (dimensionsofdentalhygiene.com)

Planktonic replication is essential for biofilm formation by Legionella pneumophila in a complex medium under static and dynamic flow conditions. (nih.gov)

The increase of the biofilm resistance to disinfectants, with the rise of the incubation time, was dependent on both growth temperature and disinfectant product. (researchgate.net)
The Assay was validated for testing the effectiveness of hard surface disinfectants formulated to kill biofilm. (dentistryiq.com)

Some of the red-labeled bacterial cells got stuck in the mesh, creating an even-larger web of biofilm streamers (seen in red in the movie) that ensnared more cells. (nih.gov)
Biofilms are structured clusters of bacterial cells embedded in a polymeric matrix and attached to a surface [ 14 ]. (hindawi.com)

An aim of this FOA is to link clinical experts, such as dentists, physicians, and nurses, with basic scientists such as immunologists, microbiologists, material scientists, mathematicians to better identify the clinical problems associated with host response to microbial biofilm associated infection. (nih.gov)
For example, a search using PubMed for 2004-2016 identified 488 publications when using the search terms "biofilm and healthcare-associated infection" and 7,303 publications using the search terms "biofilm and public health," compared to 73 and 1,459 publications, respectively, for 1992-2003. (cdc.gov)
Nicole Slacik, BS, executive vice president of health care for EvaClean Infection Prevention Solutions, spoke with Infection Control Today ( ICT ) about Candida auris ( C auris ) and how biofilms make it and other "superbugs" even more dangerous. (infectioncontroltoday.com)
Biofilms aid its survival and persistence in the environment and are a mechanism for infection transfer. (infectioncontroltoday.com)
The cells extended tendrils into the biofilm and began to engulf the bacteria, as they would when faced with an infection in the body. (nih.gov)
By showing that bacterial biofilm components can promote the development of autoimmunity or worsen it, we've suggested that other treatment approaches, such as controlling an underlying infection, may be beneficial. (nih.gov)

The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
If biofilm bacteria were simply planktonic cells that had adhered to a surface, this revelation would be unimportant, but they are demonstrably and profoundly different. (nih.gov)

They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
Although limited treatment options are presently available, better understanding of the molecular biology of biofilms and their pathogenicity will likely lead to the development of novel anti-biofilm agents for clinical use. (medscape.com)
After further development, this technology could provide a new method of biofilm removal for teeth, medical implants, and medical devices. (nih.gov)
This broad-based initiative on microbial biofilms is designed to elucidate the mechanisms underlying their formation as well as development of strategies for the prevention and treatment of microbial biofilm-associated diseases. (nih.gov)
Development of a novel computer simulator of biofilm growth dynamics in porous media, which combined a continuum-based approach for fluid flow and mass transport with individual-based approaches for biofilm growth and detachment. (aiche.org)
Thus, a better understanding of processes that lead to development of biofilms and their chemical and mechanical properties is needed. (spie.org)
More researches are necessary to understand the mechanisms of biofilm formation and the influence of different parameters in their development. (nih.gov)
The aim of the project is the development of methods for the analysis and monitoring of biofilms in cooling lubrication systems of the metalworking industry. (zhaw.ch)
The waterlines of a dental unit promote bacterial growth and the development of biofilms due to the dark, long narrow tubing, slow flow rates and periods of stagnation. (airtechniques.com)
Biofilm may be a potential protection factor against acid attacks and the development of dental erosion. (bvsalud.org)

Implementing the Monarch Lines Cleaner is a safe and cost-effective way to reduce biofilm contamination. (airtechniques.com)

For example, Pseudomonas aeruginosa produces destructive proteinases when the number of these bacteria reach a high enough density in the airway biofilms of cystic fibrosis patients. (nih.gov)
Paeonol attenuates quorum-sensing regulated virulence and biofilm formation in Pseudomonas aeruginosa. (greenmedinfo.com)
Using the CMCF beamline at the Canadian Light Source (CLS) at the University of Saskatchewan (USask), the team has been able to visualize the interaction between two important proteins that are key players in Pseudomonas 's ability to make biofilm. (lightsources.org)

The need for increased research on biofilms is based on many factors: - Biofilms are remarkably difficult to treat with antimicrobials. (nih.gov)
Antimicrobials may be readily inactivated or fail to penetrate into the biofilm. (nih.gov)
The increase of growth temperature from 20 °C to 37 °C, with an incubation time of 24 h, increased the resistance of biofilms to cationic antimicrobials. (researchgate.net)

The new research shows that the complexes formed from curli amyloid and DNA in the biofilms of both Salmonella and E. coli give rise to not only inflammation, but the self-attacking antibodies of lupus. (scienceblog.com)

Microscopic and molecular interaction analyses demonstrated that polysaccharides were critical for capturing and stabilizing external RNA in biofilms, which contributes to biofilm organization. (nature.com)
however, the mechanistic basis for biofilm formation remains incompletely understood at the molecular level. (nature.com)

They can find their way to areas of the tubing to grow another biofilm colony or into a patient's mouth where they can potentially cause harm. (airtechniques.com)

Only recently has C auris fungi emerged as a dangerous biofilm-associated agent. (infectioncontroltoday.com)

For the first time it is described that lactobacilli are the predominant bacteria in biofilms, and grow intertwined with Candida. (rug.nl)
The interaction between Candida en lactobacilli was studied in more detail in vitro. (rug.nl)
How Does Biofilm Make Candida auris Worse? (infectioncontroltoday.com)

A team from the Technical University of Munich (TUM) describes processes from the field of biology that utilize biofilms as 'construction workers' to create structural templates for new materials that possess the properties of natural materials. (nanowerk.com)
Following an assessment of recent discoveries concerning the formation and distribution of plastic-associated biofilms, our knowledge concerning their ecological roles and ability to drive processes including polymer biodegradation is considered. (springer.com)
300 proteins in V. cholerae at 22°C, increasing the production of biofilm-related proteins including the key transcriptional activators VpsR and VpsT, as well as proteins important for diverse cellular processes. (elifesciences.org)

Certain species of bacteria communicate with each other within the biofilm. (nih.gov)
In short, bacteria found at the bottom of the biofilm look and act different than species located at the surface. (nih.gov)

Anatomy1

Organisms13

Diseases2

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes13

Disciplines and Occupations1

Technology, Industry, Agriculture6

Health Care4

Ability to form biofilms4

Promoted biofilm formation1

Staphylococcus4

Microorganisms12

Pathogens8

Prevent biofilm formation3

Infections25

Role of biofilms3

Known as biofilms1

Genes11

Antibiotics14

Organisms15

Surfaces18

Contribute to biofilm2

Bacteria within biofilms2

Abstract1

Investigating biofilm development1

Adherent2

Treatments7

Anti-biofilm activity1

Extracellular matrix3

Microbes1

Field of biofilm2

Structure of biofilms1

Researchers16

Antibiotic resistance3

Aureus biofilms2

Forms biofilms1

Fungal biofilms2

Mature biofilms1

Intact biofilms1

Produce biofilms2

Tissues2

Bacterium2

Phenotype2

Eradicate1

Dendritic cells3

Clinically-relevant2

Formation of bacterial1

Chronic4

Form20

Inhibition2

Resistant3

Prosthetic2

Dental Unit Waterlines1

Legionella1

Disinfectants2

Bacterial cells2

Infection6

Planktonic cells2

Development10

Contamination1

Pseudomonas3

Antimicrobials3

Salmonella1

Molecular2

Colony1

Fungi1

Candida3

Processes3

Species2