• Epifluorescence and scanning electron microscopy revealed that S. aureus rapidly forms mature biofilm in wounds within 24 hours of inoculation, with persistence of biofilm viability over time seen through serial bacterial count measurement and laser scanning confocal imaging at different time points postwounding and inoculation. (northwestern.edu)
  • Scanning electron microscopy and confocal laser scanning microscopy were used to assess biofilm formation, while epifluorescence and qPCR methods were effectively used to determine cell contents. (pucv.cl)
  • Much of the work in the last 2 decades has relied on tools such as scanning electron microscopy (SEM) or standard microbiologic culture techniques for biofilm characterization. (cdc.gov)
  • 3 ) used scanning and transmission electron microscopy to examine biofilms on trickling filters in a wastewater treatment plant and showed them to be composed of a variety of organisms (based on cell morphology). (cdc.gov)
  • Quantification of marine viruses was originally performed using transmission electron microscopy but has been replaced by epifluorescence or flow cytometry. (wikipedia.org)
  • Two major thrusts in the last decade have dramatically impacted our understanding of biofilms: the utilization of the confocal laser scanning microscope to characterize biofilm ultrastructure, and an investigation of the genes involved in cell adhesion and biofilm formation. (cdc.gov)
  • Inflammatory markers confirmed that the biofilm phenotype creates a characteristic, sustained, low-grade inflammatory response, and that over time biofilm impairs epithelial migration and granulation tissue in-growth, as shown histologically. (northwestern.edu)
  • We have established and validated a highly quantitative, reproducible in vivo biofilm model, while providing evidence that the biofilm phenotype specifically contributes to profound cutaneous wound healing impairment. (northwestern.edu)
  • Rediscovery of a microbiologic phenomenon, first described by van Leeuwenhoek, that microorganisms attach to and grow universally on exposed surfaces led to studies that revealed surface-associated microorganisms (biofilms) exhibited a distinct phenotype with respect to gene transcription and growth rate. (cdc.gov)
  • The existence of viruses in the ocean was discovered through electron microscopy and epifluorescence microscopy of ecological water samples, and later through metagenomic sampling of uncultured viral samples. (wikipedia.org)
  • 2017). Detection and discrimination of biofilm populations using locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH) . (up.pt)
  • 2016). FISHji: New ImageJ macros for the quantification of fluorescence in epifluorescence images . (up.pt)
  • Bacterial type IV pili are critical for diverse biological processes including horizontal gene transfer, surface sensing, biofilm formation, adherence, motility, and virulence. (biorxiv.org)
  • Type IV pili are ubiquitous surface appendages in Gram-negative bacteria that promote diverse activities including attachment, virulence, biofilm formation, horizontal gene transfer, and twitching motility [ 1 - 5 ]. (biorxiv.org)
  • Does biofilm formation hinder photosythesis? (stackexchange.com)
  • Dermal punch wounds were created in New Zealand rabbit ears, and used as uninfected controls, or inoculated with green fluorescent protein-labeled Staphylococcus aureus to form wounds with bacteria predominantly in the planktonic or biofilm phase. (northwestern.edu)
  • Study of the evolution of allochthonous bacteria in natural aquatic environments. (ac.be)
  • Moreover, microscopic analysis indicated a disruption and almost complete removal of the biofilms after F1/2 treatment. (bvsalud.org)
  • An established biofilm structure comprises microbial cells and EPS, has a defined architecture, and provides an optimal environment for the exchange of genetic material between cells. (cdc.gov)
  • A biofilm is an assemblage of surface-associated microbial cells that is enclosed in an extracellular polymeric substance matrix. (cdc.gov)
  • Van Leeuwenhoek, using his simple microscopes, first observed microorganisms on tooth surfaces and can be credited with the discovery of microbial biofilms. (cdc.gov)
  • As early as 1973, Characklis ( 4 ) studied microbial slimes in industrial water systems and showed that they were not only very tenacious but also highly resistant to disinfectants such as chlorine. (cdc.gov)
  • A growing body of evidence suggests that in addition to hypoxia, ischemia-reperfusion injury, and intrinsic host factors, bacterial biofilms represent a fourth major pillar in chronic wound pathogenesis. (northwestern.edu)
  • Our model highlights the importance of bacterial biofilms in chronic wound pathogenesis, providing an in vivo platform for further inquiry into the basic biology of bacterial biofilm-host interaction and high-throughput testing of antibiofilm therapeutics. (northwestern.edu)
  • To assess the biofilm removal on fabrics, (1) a detergent-based, (2) enzyme-based, and (3) combined formulation of both detergent and enzymes (F1/2) were applied. (bvsalud.org)
  • Biofilms are often tolerant towards routine cleaning and disinfection processes. (bvsalud.org)
  • Cells may also communicate via quorum sensing, which may in turn affect biofilm processes such as detachment. (cdc.gov)
  • A greater understanding of biofilm processes should lead to novel, effective control strategies for biofilm control and a resulting improvement in patient management. (cdc.gov)
  • The current study proposes a novel test model for the growth and removal of biofilms on textiles with Pseudomonas fluorescens and the opportunistic nosocomial pathogen Pseudomonas aeruginosa as model organisms. (bvsalud.org)
  • Biofilms have great importance for public health because of their role in certain infectious diseases and importance in a variety of device-related infections. (cdc.gov)
  • Biofilm-associated cells can be differentiated from their suspended counterparts by generation of an extracellular polymeric substance (EPS) matrix, reduced growth rates, and the up- and down- regulation of specific genes. (cdc.gov)
  • By using a specific polysaccharide-stain called Ruthenium red and coupling this with osmium tetroxide fixative, these researchers were also able to show that the matrix material surrounding and enclosing cells in these biofilms was polysaccharide. (cdc.gov)
  • These balls have a green epifluorescence and they are in mesophyll cells of rice leaves. (stackexchange.com)
  • In that study, threshold amounts of trehalose - a limiting, produced carbon-resource, controls the emergence and self-organization of cells exhibiting the glycolytic state, serving as a carbon source that fuels glycolysis (Varahan et al. (elifesciences.org)
  • In this study, we succeeded in detecting microplastic (MPs) particles and microplastic fibers, but also nanoplastic (NPs). (plos.org)
  • After a 72 h incubation period, F. psychrophilum had formed mature biofilms composed by dense, multilayered clusters of short bacilli on stainless steel, polystyrene, and polycarbonate. (pucv.cl)
  • These biofilm microorganisms have been shown to elicit specific mechanisms for initial attachment to a surface, development of a community structure and ecosystem, and detachment. (cdc.gov)
  • 5 ) in 1978 put forth a theory of biofilms that explained the mechanisms whereby microorganisms adhere to living and nonliving materials and the benefits accrued by this ecologic niche. (cdc.gov)
  • Since that time, the studies of biofilms in industrial and ecologic settings and in environments more relevant for public health have basically paralleled each other. (cdc.gov)
  • Biofilm growth was poor on polyurethane surfaces. (pucv.cl)
  • Microorganisms attach to surfaces and develop biofilms. (cdc.gov)
  • In recent years, studies have highlighted the importance of the size of plastic particles, and the impact of this pollution source on the environment. (plos.org)
  • 2010). Biological activity of heterologous murine interleukin-10 and preliminary studies on the use of a dextrin nanogel as a delivery system . (up.pt)
  • The aim of this study was to employ the CDC Biofilm Reactor to evaluate the capacity of F. psychrophilum to form biofilms on stainless steel, polystyrene, polyurethane, and polycarbonate. (pucv.cl)
  • form robust biofilms on woven cellulose that can be efficiently removed via F1/2, proven by a significant reduction (p (bvsalud.org)
  • As they can grow on fabrics in household or healthcare settings, resulting in odors and serious health problems, it is necessary to contain biofilms through eradication strategies. (bvsalud.org)
  • This systematic study could guide the design of next-generation ruthenium-based complexes for enhanced antibacterial photodynamic strategies. (bvsalud.org)
  • In our study, we show that FISH-based diagnosis of individual vital T. whipplei in FFPE specimens is feasible and can be considered as ancillary diagnostic tool for the diagnosis of WD in FFPE material. (frontiersin.org)
  • We have developed a microfluidic device to study the degradation of single microparticles. (bvsalud.org)
  • The enzymatic degradation of poly (1,4-butylene adipate-co-terephthalate) (PBAT) microparticles was studied using Novozym 51032 cutinase. (bvsalud.org)
  • Here, we study the type IVa competence pilus of Vibrio cholerae as a model system to define the role of PilU. (biorxiv.org)
  • This study shows that plastic pollution must be considered in environmental studies even in the most pristine locations. (plos.org)
  • The following projects were ranked as internationally excellent or world-leading as Impact Case Studies for REF 2021 , the UK Government's system for assessing the quality of research in UK higher education institutions. (port.ac.uk)
  • The funders play no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. (plos.org)