• The inhibition of quorum sensing, called quorum quenching, may disrupt oral biofilm formation, which is the main etiological factor of oral diseases, including periodontitis. (mdpi.com)
  • The concentration of the chemicals tested as anti-biofilm agents was chosen based on cytotoxicity assays: the highest non-cytotoxic chemical concentration was used for biofilm inhibition assays, with dendrimer concentration 10-fold higher than polyamino-phenolic ligands. (frontiersin.org)
  • Biofilms are composed of microorganisms attached to a solid surface or floating on top of a liquid surface. (nature.com)
  • Disinfection methods currently used in water systems have been shown to be ineffective against legionella over the long-term, allowing recolonization by the biofilm-protected microorganisms. (frontiersin.org)
  • Comparing the TEMPO-CNF behavior with that of alginate, the scientists observed that the nanofibrils were as biocompatible as the established matrix but yielded more hydrogen gas. (chemistryviews.org)
  • This correlates with the finding that pre-elafin/trappin-2 and elafin bind to DNA in vitro and attenuate the expression of some P. aeruginosa virulence factors, namely the biofilm formation and the secretion of pyoverdine. (biomedcentral.com)
  • In this study, the anti-biofilm activity of previously fabricated polyamino-phenolic ligands and polyamidoamine dendrimers was investigated against legionella mono-species and multi-species biofilms formed by L. pneumophila in association with other bacteria that can be found in tap water ( Aeromonas hydrophila , Pseudomonas aeruginosa , Escherichia coli , Klebsiella pneumoniae ). (frontiersin.org)
  • To improve our understanding, we need access to the chemical cell-to-cell communication (quorum sensing, QS), to enable monitoring of changes in biofilms, specifically the competition of bacterial communities for resources and space, and the population density-dependent change from non-virulent to virulent. (europa.eu)
  • Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. (frontiersin.org)
  • Further, we use reversible reactions to mimic the diffusion in biofilms. (nature.com)
  • Nocodazole did not greatly impact the diffusion behavior of NPs within either cell line. (upenn.edu)
  • Oscillations are thought to be advantageous for biofilms to cope with nutrient starvation and chemical attacks. (nature.com)
  • Atomic force microscopy (AFM) was employed to study the structure-mechanical property relationship for these mono- and bi- layered polymer grafts at pH 7.4 and pH 4.0, corresponding to physiological and biofilm formation conditions, respectively. (upenn.edu)
  • Cells in the interior, on the other hand, depend on the leftover glutamate that diffuses inwards in the biofilm, but do not lose gaseous molecules as rapidly as the peripheral cells. (nature.com)
  • These data suggest that the studied compounds, especially dendrimers, could be considered as novel molecules in the design of research projects aimed at the development of efficacious anti-biofilm disinfection treatments of water systems in order to minimize legionellosis outbreaks. (frontiersin.org)
  • The biofilm-based reactors allow for more efficient light utilization and a significant decrease of water usage in the system, says Yagut Allahverdiyeva-Rinne, a professor of molecular plant biology at the University of Turku, who, together with Tekla Tammelin of VTT, led the study. (chemistryviews.org)
  • Mechanical removal of biofilm on titanium discs: An in vitro study. (gu.se)
  • Regulation of biofilm growth is complex and still largely elusive. (nature.com)
  • In experiments using a microfluidics chamber, oscillations were observed in the growth of Bacillus subtilis 4 which was supplied with glutamate on one end of the chamber while the waste products of the biofilm were washed off at the other end at a constant rate. (nature.com)
  • The peripheral cells depend on the ammonia diffusing from the interior for their growth. (nature.com)
  • Immobilized in the biofilm, the cells consume less energy for growth and can act as true biocatalysts, converting solar energy into chemicals, she adds. (chemistryviews.org)
  • Legionnaires' disease is a potentially fatal pneumonia caused by Legionella pneumophila , an aquatic bacterium often found within the biofilm niche. (frontiersin.org)
  • Most direct costs were associated with pathogens found in biofilms. (cdc.gov)
  • Porosity is especially important for biofilms because vital nutrients and gases can diffuse to and from the cells, allowing high cell concentrations and production activities. (chemistryviews.org)
  • Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
  • The organization and extent of polymerization of the actin within each cell controls the confinement the NPs experience and their ability to diffuse. (upenn.edu)
  • Herein, the surface interactions between polymer grafts and the negatively-charged silica colloid attached to an AFM lever are considered as representative interactions between the antibacterial coating and a bacteria/biofilm. (upenn.edu)
  • As such, surface modification with ceramic coatings is required in order to achieve proper biomedical features and enhance their overall behavior in the human body. (infim.ro)
  • Today, scientists and engineers are more and more abandoning traditional suspension-culture-based reactor technologies in favor of systems based on immobilized biofilms. (chemistryviews.org)
  • In man-made water systems microbial biofilms increase the resistance of legionella to disinfection, posing a significant threat to public health. (frontiersin.org)
  • The peripheral cells of the biofilm have the advantage of direct access to glutamate. (nature.com)
  • This communication alters gene expression and allows bacteria to mount coordinated responses to their environments, in a manner that is comparable to behavior and signaling in higher organisms. (wikipedia.org)
  • Bacterial biofilm formation is a major persistent threat, as it increases morbidity and mortality, thereby imposing heavy economic pressure on the healthcare sector. (frontiersin.org)
  • Bacterial biofilms also strengthen biofouling, affecting shipping functions, and the offshore industries in their natural environment. (frontiersin.org)
  • The development of intact biofilms is very critical for the spreading and persistence of bacterial infections in the host. (frontiersin.org)
  • Therefore, this review discusses the current understanding of antibiotic resistance mechanisms in bacterial biofilm and intensely emphasized the novel therapeutic strategies for combating biofilm mediated infections. (frontiersin.org)
  • Bacterial biofilms can attach to various materials such as metals, glass surfaces, plastic wares, tissues, and clinical devices. (frontiersin.org)
  • Nanomaterials for the treatment of bacterial biofilms. (scielo.br)
  • 1.1 Introduction Dental Caries is a bacterially based disease that progresses when acid produced by bacterial action on dietary fermentable carbohydrates diffuses into the tooth and dissolves the carbonated hydroxyapatite mineral resulting in demineralization. (ericlowitt.com)
  • Most direct costs were associated with pathogens found in biofilms. (cdc.gov)
  • Characterization of antimicrobial efficacy of photocatalytic polymers against food-borne biofilms. (uoguelph.ca)
  • Multifunctional nanosystems have offered unique benefits related to nano-sized particles, including large surface area, dental biofilm penetration ability, and controlled drug delivery. (scielo.br)
  • public health programs are needed to prevent biofilm-associated diseases. (cdc.gov)
  • Biofilm referred to the intricate three-dimensional (3-D) aggregation of bacteria attached to a surface and buried inflexibly in an Extracellular Polymeric Substance (EPS) matrix. (frontiersin.org)
  • In order to accomplish this, the activity of the biofilm itself has to be assessed. (nih.gov)
  • and plant extracts with biofilm inhibitory activity. (medscape.com)
  • This approach would require more complete knowledge of the biofilm and the factors influencing the local environment of the demineralized enamel. (nih.gov)
  • They diffuse in and out of cells by both passive transport and active transport mechanisms. (wikipedia.org)
  • The forthcoming experimental studies will focus on these recent therapeutic strategies that may lead to the development of effective biofilm inhibitors than conventional treatments. (frontiersin.org)
  • Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)