However, as a way to counteract this inhibitory effect, Farnesol may be one way that Candida has found to fight back. (bepress.com)
Quorum-sensing1
A strain lacking CHK1 that fails to respond to the quorum-sensing molecule farnesol had the same response as did the wild type. (who.int)
Bacteria8
If neither bacteria possess a functional copy of the LuxS gene they cannot form a biofilm. (wikipedia.org)
However, if either one of the bacteria has the LuxS gene they can form biofilms suggesting again this molecule is used for communication between unrelated species. (wikipedia.org)
Bacteria within biofilms are enmeshed in an exopolysaccharides (EPS)-rich matrix. (rochester.edu)
Furthermore, EPS-embedded bacteria also create highly protected and acidic microenvironments that promote cariogenic biofilm build-up and acid-dissolution of tooth enamel. (rochester.edu)
Dental caries results from the interaction of specific bacteria with constituents of the diet within a biofilm termed 'dental plaque' 1 . (bvsalud.org)
Biofilm bacteria attach to surfaces and are surrounded by an extracellular matrix called the extracellular polymeric substance (EPS) (Costerton et al. (springeropen.com)
Bacteria also form biofilms, and the pH component is critical in mediating the entire skin microbiome. (gestaltreality.com)
Cariogenic5
Abstract: The development of novel chemotherapeutic approaches against cariogenic biofilms is challenging. (rochester.edu)
We demonstrated that these agents in combination severely compromise EPS-matrix assembly and cariogenic biofilm development, resulting in a highly effective anti-caries therapy in vivo. (rochester.edu)
Then, Aim 2 will evaluate the efficacy of optimized NPCs containing Myr and Far with fluoride (CT-NPC) using our in vitro cariogenic biofilm model. (rochester.edu)
Aim 3 will evaluate the efficacy of the developed CT-NPC in disrupting cariogenic biofilms and reducing dental caries in vivo using a rodent model of dental caries under clinically-relevant topical treatment regimen. (rochester.edu)
The cells did not produce farnesol and did not respond to exogenous farnesol, and they were resistant to the highest tested levels of amphotericin B and four of the azole antifungals. (nebraska.edu)
Planktonic4
Biofilms that formed in microtiter plate wells, like high-density planktonic organisms, were resistant to drugs. (who.int)
A fluconazole-resistant strain showed greater resistance at high planktonic cell density, in biofilm, and in resuspended biofilm than did low-density planktonic or biofilm organisms. (who.int)
Effects of extracts from Italian medicial plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. (univpancasila.ac.id)
2016). Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells . (up.pt)
14. Ando E, Monden K, Mitsuhata R, Kariyama R and Kumon H. Biofilm formation among methicillin-resistant Staphylococcus aureus isolates from patient with urinary tract infection. (univpancasila.ac.id)
2011). SYBR green as a fluorescent probe to evaluate the biofilm physiological state of staphylococcus epidermidis, using flow cytometry . (up.pt)
Resistance5
We investigated the contribution of cell density to biofilm phenotypic resistance. (who.int)
When biofilms were resuspended before testing, phenotypic resistance remained, but organisms, when diluted to 1 x 10(3) cells/ml, were susceptible. (who.int)
These observations suggested that cell density has a role in the phenotypic resistance of biofilm, that neither the drug efflux pumps tested nor quorum sensing through Chk1p contributes to resistance, and that azole drug tolerance at high cell density differs mechanistically from tolerance at low cell density. (who.int)
We suggest that the anaerobic growth of C. albicans may contribute to the trailing end point phenomenon and the resistance of C. albicans biofilms to antifungal drugs. (nebraska.edu)
Scanning electron m3
Scanning electron microscopy (SEM) has been successfully used to image biofilms because of its high resolution and magnification. (springeropen.com)
Many early biofilm studies used scanning electron microscopy (SEM), which showed in detail the surface morphologies of microbial biofilms and their structure. (springeropen.com)
SEM and Confocal laser scanning electron microscopy (CLSM) are used to analyze changes in the biofilm integrity as a result of treatment [ 20 ] . (encyclopedia.pub)
Mutans1
The most frequent oral infections include gingivoperiodontal diseases including gingivitis and periodontitis, are caused by dental plaque, which is a S. mutans produced biofilm[ 9 - 11 ]. (biomedcentral.com)
Virulence1
Thus, we will investigate how CT-NPC disrupts these virulence properties more effectively than CT using novel methods to assess spatiotemporal development of EPS matrix, acidic pH niches and gene expression in situ within intact 3D biofilms. (rochester.edu)
Cells1
Direct SEM observation clearly revealed that bacterial cells in a biofilm were extensively surrounded by fibrous or amorphous matrices, which represented the EPS (Marrie and Costerton 1984 ). (springeropen.com)
20011
1999 ). Ninety percent of the dry weight mass of biofilms is composed of EPS, and the other major component of the biofilm matrix (up to 97%) is water (Sutherland 2001 ). (springeropen.com)
Microtiter1
16. Djordjevic D, Wiedmann M, McLandsborough LA. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. (univpancasila.ac.id)
Bacterial1
The incorporation of tt-farnesol into the adhesive system significantly reduced bacterial viability and the production of extracellular insoluble polysaccharides (EIP). (signicent.com)
Effectiveness1
The funding will enable further development of the approach, including coencapsulation and delivery of synergistic anti-biofilm drugs and testing the effectiveness of the approach in rodent models. (rochester.edu)
In addition, retention of these agents at tooth-biofilm interface could be enhanced to maximize their efficacy in vivo. (rochester.edu)
Resistant1
A strain lacking drug efflux pumps CDR1, CDR2, and MDR1, while susceptible at a low azole concentration, was resistant at high cell density and in biofilm. (who.int)
Formation1
AI-2 is required for the biofilm formation in P. ginivalis and S. gordonii. (wikipedia.org)
Drugs1
FK506, reported to abrogate tolerance to azole drugs at low cell density, had no effect on tolerance at high cell density and in biofilm. (who.int)
The antibacterial and bonding capacity of a commercial universal dental adhesive incorporated with the antibacterial agent tt-farnesol creating 3 experimental adhesives: 0.38% (v/v), 1.90% (v/v), and 3.80% (v/v), plus a control (no incorporation of tt-farnesol) were accessed. (signicent.com)
Matrix1
Compared with samples prepared by the conventional method, the ionic-liquid-treated samples do not exhibit a fibrous extracellular matrix structure and cracking on the biofilm surface. (springeropen.com)
Biological1
However, conventional SEM requires dehydration and metal coating of biological samples before observation, and because biofilms consist mainly of water, sample dehydration may influence the biofilm structure. (springeropen.com)
Highest1
Results showed that extract or fraction samples which have the highest biofilm inhibition activity toward MRSA were hexane fraction of kapulaga IC50 0.23±0.01 mg/mL, temu putri IC50 0.45±0.03 mg/mL and the ethyl acetate fraction of senggugu IC50 0.35±0.022 mg/mL. (univpancasila.ac.id)
State1
To avoid the draconian process of total sample dehydration required in conventional SEM, environmental SEM (ESEM), which can be used to visualize specimens in a wet or partially hydrated state, has been used to observe biofilm samples (Darkin et al. (springeropen.com)
Agents2
To overcome these remarkable challenges, our previous NIH supported (DE018023) studies developed a potent anti-caries approach by combining food-derived antibiofilm agents (myricetin and farnesol) with fluoride. (rochester.edu)
We will focus on increasing the kinetics of NPC pH-responsive drug release to ensure maximal release of the agents at pH consistent with the acidic biofilm milieu. (rochester.edu)
Process1
Pharmaceutical antifungals can be used but often have limited impact and will need to be used for extended periods of time due to the very slow process of moving through the biofilm. (gestaltreality.com)
Important1
To address these hurdles, we have developed pH-responsive nanoparticle carriers (NPC) capable of co-encapsulating myricetin (Myr) and farnesol (Far) which were completely water-soluble, important towards practical formulations for human use. (rochester.edu)