Biofilm communities biofilms. Medical search. Frequent questions

Instead, the cells take on different roles, including the initial free-swimming bacteria, the scaffolding-producing cells, and, eventually, a third type of cell that send out spores to start new biofilms. (harvard.edu)
Moreover, in mixed-species biofilms, the waste products of one type of bacteria can be used by another species. (harvard.edu)
In addition, the diversity of bacteria in a colony can make it difficult for a single treatment to destroy the biofilm. (harvard.edu)
Microbes such as bacteria tend to live in complex colonies called biofilms, where they can resist antibiotics and cause more problems for the immune system. (sciencedaily.com)
Biofilms comprising millions of bacteria are at the root of many serious chronic infectious diseases such as cystic fibrosis and periodontal disease, as well as industrial contamination, biofouling and biocorrosion. (sciencedaily.com)
In cystic fibrosis, excess mucus production in the airways gives sanctuary to bacteria such as Pseudomonas aeruginosa, which actually mop up the dead carcasses of white blood cells sent by the immune system, enabling them to construct their protective biofilm coat. (sciencedaily.com)
The ESF workshop also highlighted greater understanding of the complex interactions within biofilms, which often comprise not just one species of bacteria, but a whole host of different micro-organisms, including archaea, protozoa, fungi, and even tiny metazoa actually comprising multiple cells. (sciencedaily.com)
Biofilms are a mixture of complex communities of organisms mostly composed of diverse bacteria that vary depending on the surrounding environmental conditions induced by physical and chemical factors. (intechopen.com)
Observation of biofilms dates back to the seventeenth century when Antonie van Leeuwenhoek observed bacteria from the plaque biofilm of his teeth under his primitive microscope [ 2 ]. (intechopen.com)
Flemming, H.-C. & Wuertz, S. Bacteria and archaea on Earth and their abundance in biofilms. (nature.com)
the bacteria organize themselves into a coordinated functional community. (wikipedia.org)
The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
Hydrophobicity can also affect the ability of bacteria to form biofilms. (wikipedia.org)
Both of the bacteria from this study are associated with plant roots, and understanding their interactions using DAPG and other secreted compounds could be important for creating healthy microbial soil communities for plants to grow in, possibly boosting agricultural yields, said Shank. (sciencedaily.com)
A biofilm is a collection of bacteria and/or fungi that exists in a multicellular or community form, encased in an extracellular polysaccharide matrix that they themselves synthesise. (thepigsite.com)
Formation of a biofilm starts with adherence of bacteria followed by production of the polysaccharide matrix. (thepigsite.com)
Other bacteria, other micro-organisms and debris become incorporated in the biofilm. (thepigsite.com)
Bacteria within a biofilm communicate by quorum sensing leading to phenotypic modifications and changes in gene expression. (thepigsite.com)
Bacteria within the biofilm are protected from disinfectants and have a fundamentally reduced antibacterial sensitivity compared with planktonic, free-living, bacteria. (thepigsite.com)
Some bacteria like to live in community. (l3s.de)
In the BacData project, the researchers are investigating two clinically highly relevant biofilm associated infections: the lung infection in cystic fibrosis patients caused by Pseudomonas bacteria and the implant infection as an example of frequently occurring multi-species biofilm infections. (l3s.de)
Based on the comparison of clean water and dirty water control bath toys, we argue that bath toy biofilms are influenced by (1) the organic carbon leaching from the flexible plastic material, (2) the chemical and biological tap water quality, (3) additional nutrients from care products and human body fluids in the bath water, as well as, (4) additional bacteria from dirt and/or the end-users' microbiome. (discovermagazine.com)
Therefore, biofilm matrix gives the additional resistance power to bacteria which makes them to not only tolerate harsh conditions but also resistant to antibiotics which lead to the emergence of bad bugs infections like multi drug resistant, extensively drug resistant and totally drug resistant bacteria. (biomedcentral.com)
But let's start with the what: Biofilm is a community of bacteria. (shieldhealthcare.com)
Traditional lab testing cannot measure bacteria in biofilms. (shieldhealthcare.com)
These are products that you can put into the wound, and it will help keep out and kill bacteria and decrease the biofilm. (shieldhealthcare.com)
The present authors hypothesised that bronchoscopy with protected specimen brush may sample biofilm-forming bacteria adherent to the airway wall, whereas traditional sputum collection may not. (ersjournals.com)
Retrieved bacteria were genotyped, and grown in planktonic cultures and as biofilms, and susceptibilities to individual antibiotics and to antibiotic combinations were determined. (ersjournals.com)
Susceptibility to single antibiotics and to antibiotic combinations were not different between planktonically or biofilm-grown bacteria derived from sputum, as compared to those obtained by bronchoalveolar lavage and protected brush. (ersjournals.com)
One problem with relying on sputum samples to guide therapy is that biofilm bacteria may not be adequately sampled via sputum, since sputum expectoration only samples P. aeruginosa that have detached and dispersed from the airway biofilm 5 , 6 . (ersjournals.com)
However, studies have not previously been done to determine whether bacteria retrieved from sputum are more susceptible to antibiotics compared to bacteria retrieved directly from the airway biofilm. (ersjournals.com)
Thus, it is possible that PSB may directly recover P. aeruginosa bacteria that have aggregated as biofilms and adhered to the surface of the airway. (ersjournals.com)
In contrast to the free-living, or planktonic, bacteria commonly studied in the lab, most prokaryotes in nature settle down in complex communities, often consisting of several interacting species. (the-scientist.com)
Biofilms form when groups of bacteria cover themselves in a sticky mixture of sugars, protein, and DNA. (the-scientist.com)
Within the biofilm, bacteria take on different responsibilities. (the-scientist.com)
And other bacteria defend the community, building molecular weapons that shoot competitors with toxins. (the-scientist.com)
Bacteria are able to grow adhered to almost every surface, forming architecturally complex communities termed biofilm. (igem.org)
In biofilm, cells grow in multicellular aggregates that are encased in an extracellular matrix produced by the bacteria themselves (1). (igem.org)
In the biofilm formation bacteria can talk each other via some signal mechanisms. (igem.org)
Biofilms - surface-associated communities of bacteria - are the leading cause of microbial infection worldwide and kill as many people as cancer does. (princeton.edu)
To prevent UTIs and reduce unnecessary antibiotic use, NCEZID staff and Georgia Institute of Technology (Georgia Tech) developed a coating made of bacteriophages (phages), or viruses that selectively kill bacteria to prevent biofilm development on catheters. (cdc.gov)
The phages effectively fight the development of biofilms, avoid potential dangers of antibiotic overuse, and can target multiple infectious bacteria at once, which might otherwise require a combination of antibiotics. (cdc.gov)
Biofilms have been found to be one of the most common growth conditions for bacteria in nature. (bvsalud.org)
These carbohydrates act as nutrients for biofilm bacteria, especially acidogenic and aciduric species. (bvsalud.org)

Microorganisms attach to surfaces and develop biofilms. (cdc.gov)
Rediscovery of a microbiologic phenomenon, first described by van Leeuwenhoek, that microorganisms attach to and grow universally on exposed surfaces led to studies that revealed surface-associated microorganisms (biofilms) exhibited a distinct phenotype with respect to gene transcription and growth rate. (cdc.gov)
These biofilm microorganisms have been shown to elicit specific mechanisms for initial attachment to a surface, development of a community structure and ecosystem, and detachment. (cdc.gov)
Van Leeuwenhoek, using his simple microscopes, first observed microorganisms on tooth surfaces and can be credited with the discovery of microbial biofilms. (cdc.gov)
5 ) in 1978 put forth a theory of biofilms that explained the mechanisms whereby microorganisms adhere to living and nonliving materials and the benefits accrued by this ecologic niche. (cdc.gov)
Biofilms are complex, highly dynamic, structured ecosystems formed by a community of different microorganisms living attached to inert or living surfaces and embedded in a matrix designated extracellular polymeric substance (EPS). (intechopen.com)
Several other microorganisms come after entering in this very competitive ecosystem where quorum sensing and antibiotic resistance are determinants in the development of the community. (intechopen.com)
A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
Sprinklers and showerheads are known to support the development of microbial biofilms, which can be a source of infection by pathogenic microorganisms. (huji.ac.il)
The extracellular polymeric matrix is an important structural component of biofilm and it plays an important role in the attachment and colonization of microorganisms on a surface also acts as a diffusion barrier to small molecules. (igem.org)
Due to the survival advantages conferred by the biofilm morphology to microorganisms over the planktonic morphology, biofilms are particularly concerning from a planetary protection perspective. (usda.gov)
Endpoint community analysis showed a significant selection of microorganisms by the media. (usda.gov)
In the United States, urinary catheters cause roughly 75% of UTIs , and biofilms - complex communities of microorganisms that irreversibly attach to a surface - are often the cause. (cdc.gov)
Surprisingly, previous 16S rRNA gene surveys of biofilm samples revealed microbial communities dominated by sequences affiliated with nitrogen-cycling microorganisms. (lu.se)
This study aimed to identify microbial genomes with metabolic potential for novel nitrogen- and metal-cycling reactions, representing biofilm microorganisms that could link these cycles and play a role in concrete biodeterioration. (lu.se)
Our results expand the diversity of microorganisms putatively involved in nitrogen and metal cycling, and contribute to our understanding of potential biofilm impacts on built infrastructure. (lu.se)

For instance, Professor Kolter's lab at Harvard is studying how the cells switch between their various roles in different parts of the biofilm. (harvard.edu)
Just as different boroughs of a city might differ in levels of air pollution or abundance of organic supermarkets, different parts of the biofilm are exposed to wildly different levels of oxygen and nutrients. (the-scientist.com)

These critical properties are derived from the existence of the protective slimy matrix within which members of the community live, preventing attack from both the immune system and antibiotics, but at the same time shielding them from toxic contaminants while breaking down waste or effluent. (sciencedaily.com)
Indeed resistance against antibiotics is itself one of the biggest problems of all associated with biofilms, Battin noted. (sciencedaily.com)
As the biofilms are resistant to antibiotics and the immune system's white blood cells, very often the only remedy is surgery, to replace a damaged valve, which can itself cause problems. (sciencedaily.com)
Microbes form a biofilm in response to a number of different factors, which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics. (wikipedia.org)
Biofilms are notoriously resistant to antibiotics. (sciencedaily.com)
Therapeutic failures of antibiotics used to treat macrolide-susceptible Streptococcus pyogenes infections may be due to biofilm formation. (microbiologyresearch.org)
Bacterial biofilms are the major cause of chronic wound infections and are notoriously recalcitrant to treatments with antibiotics, making them difficult to eradicate. (umf.org.nz)
In addition, manuka honey exhibits anti-biofilm activity, thereby warranting the investigation of its potential as a combination therapy with antibiotics for the topical treatment of biofilm-related infections. (umf.org.nz)
Here we report the first use of MacSynergy II to investigate the response of established S. aureus (strain NCTC 8325) biofilms to treatment by combinations of Medihoney (medical grade manuka honey) and conventional antibiotics that are used for preventing or treating infections: rifampicin, oxacillin, fusidic acid, clindamycin, and gentamicin. (umf.org.nz)
Using checkerboard microdilution assays, viability assays and MacSynergy II analysis we show that the Medihoney-rifampicin combination was more effective than combinations using the other antibiotics against established staphylococcal biofilms. (umf.org.nz)
Other combinations of Medihoney and antibiotic produced an interesting array of effects: Medihoney-fusidic acid treatment showed minor synergistic activity, and Medihoney-clindamycin, -gentamicin, and -oxacillin combinations showed overall antagonistic effects when the honey was used at sub-inhibitory concentration, due to enhanced biofilm formation at these concentrations which could not be counteracted by the antibiotics. (umf.org.nz)
Biofilms are home to millions of microbes, but disrupting their interactions could produce more effective antibiotics. (the-scientist.com)
The viscoelastic properties of this biofilm contributes to its ability to evade phagocytic activities, to reduce the effectiveness of antibiotics, to develop antibiotic resistant strains, and to persist or reoccur in CF patients. (aiche.org)
The disruption of biofilm formation would have a beneficial impact on the treatment of diseased CF patient as it will make the infection more susceptible to antibiotics and host phagocytosis. (aiche.org)
Traditionally, clinicians prevent biofilms on catheters by prescribing patients a high concentration of antibiotics (prophylaxis). (cdc.gov)

On the surfaces of water pipes, these biofilms can be a serious obstacle to producing clean drinking water. (harvard.edu)
Biofilms are a major suspect in such cases, especially since they are resilient enough to live for months on gloves, floors, surgical instruments, and other hospital surfaces. (harvard.edu)
Biofilms on placer gold (Au)-particle surfaces drive Au solubilization and re-concentration thereby progressively transforming the particles. (soton.ac.uk)
The plaque biofilm, unique to the oral cavity, occurs on numerous surfaces such as the cheeks, tongue and teeth and is comprised of a sticky mass of proteins, lipids, glycoproteins, and glycolipids housing oral microbial communities with special chemical and nutritional gradients. (dentalcare.com)
Biofilms can be found in a variety of habitats, on free‐living, on the surface of other organisms or inert surfaces, both in aquatic as well as terrestrial environments. (intechopen.com)
Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
By coupling flow cytometry, microscopy and high throughput sequencing (HTS, 454 pyrosequencing) with artificial surfaces and environmental analyses, we intend to identify the contribution of biofilm community drivers at two contrasted French sites, one temperate and eutrophic (Lorient, Atlantic coast) and the other at a mesotrophic but highly contaminated bay (Toulon, North-Western Mediterranean Sea). (univ-tln.fr)
Microbial communities which form biofilms on kelp surfaces are increasingly recognized as important links to metrics of kelp health. (wwu.edu)
Mycobacteria (more specifically MI) were found in all biofilm samples, including biofilms collected from inside the MRF systems and from adjacent environmental surfaces, suggesting that biofilms may play an important role in microbial ecology in MRFs. (cdc.gov)
The matrix, which is composed of polysaccharides, proteins, nucleic acids and water, enables the biofilm to attach to the surfaces. (igem.org)
Biofilms are sessile microbial communities growing on surfaces, frequently embedded in a matrix of extracellular polymeric substances (also known as slime). (medscape.com)
These findings are consistent with a tendency of other Acinetobacter species to survive on dry surfaces, form biofilms, and contribute to nosocomial outbreaks. (medscape.com)
Recent studies have demonstrated biofilm growth over root surfaces on teeth with chronic apical periodontitis and teeth refractory to root canal treatment 12 . (bvsalud.org)

Historically, multicellular bacterial communities, known as biofilms, have been thought to be held together solely by a self-produced extracellular matrix. (nature.com)
These attached communities are known as biofilms. (thepigsite.com)

Biofilms have great importance for public health because of their role in certain infectious diseases and importance in a variety of device-related infections. (cdc.gov)
There is now universal recognition these oral infections are multifactorial in nature, with a large variety of microbial species residing in intraoral plaque biofilms, with some species being beneficial (commensal) while others being capable of producing disease (pathobionts). (dentalcare.com)
As the ESF Biofilm workshop's convenor Tom J. Battin, from the University of Vienna, pointed out, biofilms are involved in most chronic infections, including killers such as cystic fibrosis, and endocarditis in the heart. (sciencedaily.com)
More than 65 percent of hospital-acquired infections manifest as biofilms. (sciencedaily.com)
While recently reported data showed a good efficacy of rifampin against P. acnes biofilms, prospective, randomized, controlled studies are needed to confirm evidence for combination treatment with rifampin, as has been performed for staphylococcal implant-associated infections. (uzh.ch)
Biofilm-related infections represent a serious health problem, accounting for 65- 80% of all infections. (cun.es)
The infections are generally chronic and characterized by the persistence of the microorganism, due to the increased resistance of biofilms to both the immune system and antimicrobials. (cun.es)
Microbiological diagnosis and antimicrobial susceptibility studies of biofilm-related infections are complex and, nowadays, represent a challenge that clinicians and microbiologists have to address as a team in the absence of consensus or standardized protocols. (cun.es)
Biofilm-associated infections are therefore one of the greatest challenges facing medicine . (l3s.de)
BacData is intended to improve the prognosis of patients suffering from biofilm-associated infections in the long term and, in addition, to create the basis for a personalised therapy of further infectious diseases. (l3s.de)
Three recent articles [ 1 , 2 , 3 ] highlight how our understanding of bacterial persistence and recent advances in disinfection modalities may provide a means for control of biofilms and nosocomial infections. (medscape.com)

Cells may also communicate via quorum sensing, which may in turn affect biofilm processes such as detachment. (cdc.gov)
In biofilms, symbionts play major roles in the relationship among organisms by the production of bioactive molecules involved in quorum sensing signaling. (intechopen.com)
Quorum sensing plays an important role in regulating the biofilm formation. (biomedcentral.com)
The goal of this study is to investigate the effects of certain natural quorum sensing inhibitors on biofilm formation. (aiche.org)
This study correlates the activity of the quorum sensing inhibitors with viscoelastic changes in the P. aeruginosa biofilm on the air-medium interface. (aiche.org)

Two major thrusts in the last decade have dramatically impacted our understanding of biofilms: the utilization of the confocal laser scanning microscope to characterize biofilm ultrastructure, and an investigation of the genes involved in cell adhesion and biofilm formation. (cdc.gov)

This initial colony grows by reproducing to form daughter cells and trapping other microbes that become part of the biofilm. (harvard.edu)
A biofilm is any group of microbes that stick together on a surface. (sciencedaily.com)
Most microbes live in mixed communities and in many circumstances, these are attached to an environmental surface encasing themselves in an extracellular polysaccharide matrix which they themselves have produced. (thepigsite.com)
These biofilms of aggregating microbes are everywhere: on rocks, bathtubs, and medical devices. (the-scientist.com)
The slimy coating on unbrushed teeth is biofilm, and the beneficial microbes on your skin and gut often grow collectively. (the-scientist.com)

T. Bacillus subtilis biofilm formation and social interactions. (nature.com)
Our study identified a novel mechanism maintaining Bacillus subtilis and Mycobacterium smegmatis biofilms-active production of calcite minerals. (nature.com)
They found that at low concentrations, the compound, DAPG (the acronym stands for 2,4-diacetylphloroglucinol), produced by the bacterium Pseudomonas protegens, did not kill the experimental target bacterium, Bacillus subtilis, but merely prevented it from forming biofilms. (sciencedaily.com)
2). Bacillus subtilis forms biofilm whose constituent cells are held together by the extracellular matrix and one of the main matrix competent is the protein TasA which is a form of amyloid fibers and binds cells together in the biofilm (3). (igem.org)
Amyloid fibers provide structural integrity to Bacillus subtilis biofilms. (igem.org)

We studied, for the first time, the effects of mutants defective in biomineralization and calcite formation on biofilm development, resilience and morphology. (nature.com)
For the study, first author Matthew Powers, an undergraduate student in Shank's lab, used a strain of B. subtilis--a species commonly used in lab experiments--that fluoresces when genes for biofilm formation are being shut off. (sciencedaily.com)
DAPG, or the DAPG-producing P. Protogens as a protobiotic, could be used to inhibit formation of harmful biofilms. (sciencedaily.com)
Control of biofilms is difficult and addresses preventing initial formation and removing existing biofilms. (thepigsite.com)
The formation of a biofilm is not a random process. (thepigsite.com)
P. acnes causes disease through a number of virulence factors, such as biofilm formation. (uzh.ch)
To date, there are a few studies which focus on the relationship between particles and biofilm formation. (mdpi.com)
Therefore, the microbial diversity of biofilms in the different pipe materials and the effect on particle concentration on biofilm formation were investigated in this study. (mdpi.com)
In addition, some determinants for biofilm formation were assessed, using six identical bath toys under controlled conditions with either clean water prior to bathing or dirty water after bathing. (discovermagazine.com)
The present study gives a detailed characterization of bath toy biofilms and a better understanding of determinants for biofilm formation and development in systems comprising plastic materials in contact with potable water. (discovermagazine.com)
Fibrinogen induces biofilm formation by Streptococcus suis and enhances its antibiotic resistance. (microbiologyresearch.org)
iii) colony formation and biofilm maturation. (biomedcentral.com)
Metabolic activity of the bacterial biofilm communities have altered as compared to the planktonic one such as increased rates of EPS production, activation or inhibition of particular genes associated with biofilm formation and decreased growth rate [ 39 ]. (biomedcentral.com)
Formation of biofilms in mycobacteria can be defined as similar as other biofilms communities. (biomedcentral.com)
Panels of studies have reported the role of various molecules in the biofilm formation and maintenance of its composition. (biomedcentral.com)
Glycopeptidolipids are indispensable for initial surface attachment during biofilm formation in Mycobacterium smegmatis [ 111 ]. (biomedcentral.com)
During biofilm formation in B. subtilis, most cells produce and secrete ComX. (igem.org)
In this project, Microsoft is helping Princeton to better understand the mechanisms of biofilm formation by providing advanced technology that will greatly extend the type of research analysis capable today. (princeton.edu)
This in turn informs the design of subsequent experiments, to provide insight into the mechanisms of biofilm formation. (princeton.edu)
Suboptimal environmental cleaning and reprocessing of instruments is a common problem in healthcare facilities, allowing for biofilm formation and risk for nosocomial transmission. (medscape.com)
Biofilm formation and bacterial viability during the survival analysis were assessed. (medscape.com)
Alfa and colleagues [ 2 ] looked at the impact of improper positioning of the elevator lever of a duodenoscope on bacterial persistence, biofilm formation, and endoscope contamination. (medscape.com)
Biofilm formation over the dentin surface was ensured by SEM analysis. (bvsalud.org)
Cellulose membrane disks (13 mm, N=12) were used for biofilm formation (24 h) of S. mutans and L. casei , which were treated (1 min) with 0.4% RPE or 0.12% Chlorhexidine (CHX). (bvsalud.org)
The control group of biofilm formation was not submitted to any treatment. (bvsalud.org)

The goal is to reduce and prevent biofilm. (shieldhealthcare.com)
To prevent biofilm from reestablishing, or from occurring in the first place, use products that are broad-spectrum. (shieldhealthcare.com)
public health programs are needed to prevent biofilm-associated diseases. (cdc.gov)

Growth pattern of Mycobacterium species are different such as M. chelonae and M. fortuitum form biofilm as vertical and entire surface growth, respectively [ 93 ]. (biomedcentral.com)
Molecular characterization of microbial communities and quantification of Mycobacterium immunogenum in metal removal fluid s and their associated biofilms. (cdc.gov)

Biofilms are multicellular communities that were so far thought to be held together solely by a self-produced organic extracellular matrix. (nature.com)
Bacterial biofilms are matrix-bound multicellular communities. (biorxiv.org)

Bactericidal effects were found in both planktonic cultures and biofilms, although higher concentrations of manuka honey were needed to inhibit biofilms. (microbiologyresearch.org)

In this study, we established RAINBOW-seq and profiled the transcriptome of Escherichia coli biofilm communities with high spatial resolution and high gene coverage. (nature.com)
The raw data were deposited to the Gene Expression Omnibus ( GSE197541 for RAINBOW-seq data and GSE213531 for RNA-seq Δ sapD biofilm periphery and pyrimidine biosynthesis heterogeneity). (nature.com)
The aim of this study was to investigate the presence of microbial biofilms in dairy cooling systems, and to analyze their population compositions using culture-independent technique, 16S rRNA gene sequencing. (huji.ac.il)
Biofilm samples were collected on eight dairy farms from 40 sprinklers and the microbial constituents were identified by deep sequencing of the 16S rRNA gene. (huji.ac.il)
As a postdoctoral researcher in Dianne Newman 's lab at the California Institute of Technology, Dar developed a way to track gene activity across the entire biofilm with submicrometer resolution. (the-scientist.com)
In this study, culture-based methods and small subunit ribosomal RNA gene clone library approach were used to characterize microbial communities in MRF bulk fluid and associated biofilm samples collected from fluid systems in an automobile engine plant. (cdc.gov)

Biofilm-associated cells can be differentiated from their suspended counterparts by generation of an extracellular polymeric substance (EPS) matrix, reduced growth rates, and the up- and down- regulation of specific genes. (cdc.gov)
As a consequence of such coordination, the nutrient-limited region of the community maintained an unexpectedly high level of metabolism, enabling it to express many signaling genes and functionally unknown genes with potential sociality functions. (nature.com)
A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated. (wikipedia.org)
Terminal restriction fragment length polymorphism and clone library analyses of 16S rRNA genes revealed CO2‐correlated bacterial community shifts between treatments A, B and D. Observed bacterial community shifts were driven by decreases in the relative abundance of Alphaproteobacteria and increases of Flavobacteriales (Bacteroidetes) at increased CO2 concentrations, indicating pH sensitivity of specific bacterial groups. (leibniz-zmt.de)

Gold particles contained biofilms composed of prokaryotic cells and extracellular polymeric substances intermixed with (bio)minerals. (soton.ac.uk)
A cohesive structure of a multi‐layer of extracellular polymeric substances (EPS) such as polysaccharides and proteins is the base of biofilm structural organization. (intechopen.com)

Microbial communities were shaped by high temperatures, salinity and lead at Toulon by but nutrients and DOC at Lorient. (univ-tln.fr)
Related to this, in biofilm the diffusion of nutrients, vitamins, or cofactors is slower resulting in a bacterial community in which some of cells are metabolically inactive. (igem.org)
Despite the reduction in culturability over time, all strains that recovered from starvation on solid-surface experiments retained their capacity to form biofilms after rehydration, an addition of nutrients, and changing temperature. (medscape.com)

In this study, the effect of manuka honey on S. pyogenes (M28) was investigated in vitro with planktonic and biofilm cultures using MIC, MBC, microscopy and aggregation efficiency. (microbiologyresearch.org)
Manuka honey permeated 24 h established biofilms of S. pyogenes, resulting in significant cell death and dissociation of cells from the biofilm. (microbiologyresearch.org)

To assess the possible roles of biomineralization in biofilm development, we grew wild-type B. subtilis cells on media in the presence or absence of calcium acetate as a calcium source. (nature.com)
For instance, the cells within a B. subtilis biofilm that are engaged in protease production are randomly determined. (the-scientist.com)

This study evaluated the antimicrobial efficacy of 2.5% and 5.25% NaOCl, 2% gel and liquid CHX and MTAD® against Enterococcus faecalis biofilms on human dentin. (bvsalud.org)
E. faecalis has demonstrated a high resistance 20 and ability to inactivate antimicrobial agents 14 , survival capacity in harsh environments, with scarce nutrient supply and extreme alkaline pH 28 , and the capacity for growth as a biofilm on root canal walls 21 . (bvsalud.org)

Much of the work in the last 2 decades has relied on tools such as scanning electron microscopy (SEM) or standard microbiologic culture techniques for biofilm characterization. (cdc.gov)

Effectiveness of honey on Staphylococcus aureus and Pseudomonas aeruginosa biofilms. (microbiologyresearch.org)

An established biofilm structure comprises microbial cells and EPS, has a defined architecture, and provides an optimal environment for the exchange of genetic material between cells. (cdc.gov)

The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides, proteins, lipids and DNA. (wikipedia.org)

The character of biofilm communities of denture wearers, however, has been shown to be distinctive when compared with healthy non-denture-wearing individuals. (medscape.com)

This work demonstrates the presence of biofilm in dairy cooling systems which may potentially serve as a live source for microbial pathogens. (huji.ac.il)
Most direct costs were associated with pathogens found in biofilms. (cdc.gov)

There are numerous advantages to being part of a microbial community, especially improved access to food and protection from antibacterials. (harvard.edu)
The calcite-dependent patterns provide resistance to environmental insults and increase the overall fitness of the microbial community. (nature.com)
Effects of ocean acidification on microbial community composition of, and oxygen fluxes through, biofilms from the Great Barrier Reef. (leibniz-zmt.de)

Why did it take so long for scientists to recognize the true nature of these bacterial communities? (harvard.edu)
Our work provides an extended understanding of the metabolic interplay in biofilms and presents a new approach of investigating complex interactions in bacterial communities on the systems level. (nature.com)
The in-depth HTS revealed that γ- and α-proteobacteria, but also Bacteroidetes, dominated highly diversified bacterial communities with a relative low β-diversity. (univ-tln.fr)

Microorganism rebreeding and biofilm shedding enter the water body in the process of a drinking water distribution system (DWDS), which poses a threat to public health. (mdpi.com)

Biofilms can be located to almost every human body tissue and on exogenous devices such as catheters, pacemakers, prosthetic material, implants, urinary catheters, etc. (cun.es)

3 ) used scanning and transmission electron microscopy to examine biofilms on trickling filters in a wastewater treatment plant and showed them to be composed of a variety of organisms (based on cell morphology). (cdc.gov)
c Scanning electron microscopy image revealing the complex structure and composition of these bath toy biofilms. (discovermagazine.com)
Confocal scanning laser microscopy confirmed that different honey-antibiotic combination treatments could eradicate biofilms. (umf.org.nz)

Fig. 6: Local cycling of nucleotide and metabolic division of labor at biofilm periphery. (nature.com)

Biofilms can form on the teeth of most animals as dental plaque, where they may cause tooth decay and gum disease. (wikipedia.org)
A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
How do Biofilms Form? (thepigsite.com)
Such a biofilm can also form on organs and implants, such as knee and hip prostheses or dental and cochlear implants. (l3s.de)
Biofilms represent a major form of microbial life on Earth and serve as a model active nematic system, in which activity results from growth of the rod-shaped bacterial cells. (biorxiv.org)
Understanding how biofilms form could enable new strategies to disrupt them. (princeton.edu)
One of its main virulence factors is its ability to form viscous, alginate-encased biofilms within the pulmonary mucus. (aiche.org)
Bravo and colleagues [ 1 ] investigated the ability of Acinetobacter pittii strains to form biofilms after long-term desiccation if they were fed with nutrient media. (medscape.com)

A biofilm is an assemblage of surface-associated microbial cells that is enclosed in an extracellular polymeric substance matrix. (cdc.gov)
Each hamus is a long tube with three hook attachments that are used to attach to each other or to a surface, enabling a community to develop. (wikipedia.org)
All examined bath toys revealed notable biofilms on their inner surface, with average total bacterial numbers of 5.5 × 106 cells/cm2 (clean water controls), 9.5 × 106 cells/cm2 (real bath toys), and 7.3 × 107 cells/cm2 (dirty water controls). (discovermagazine.com)
Visualization of biofilms on the inner surface of bath toys. (discovermagazine.com)
Surface colonization in seawater first corresponds to the selection of specific microbial biofilm communities. (univ-tln.fr)
Clustering of communities was mainly based on the surface type and secondly the site, whereas seasons appeared of less importance. (univ-tln.fr)
This overall approach exhibited the critical significance of surface characteristics in biofilm community shaping. (univ-tln.fr)
It was the first, but not the last time I would scrape biofilm off of a surface in contact with drinking water. (lu.se)
Dental caries results from surface demineralization caused by an organized biofilm exposed to fermentable carbohydrates from the diet [1]. (bvsalud.org)

Our experiments and cell-based simulations reveal that, in the initial stages of development, the flow induces a downstream gradient in cell orientation, causing asymmetrical droplet-like biofilm shapes. (biorxiv.org)
Princeton is contributing world-leading expertise in experiments and modelling of microbial biofilms. (princeton.edu)

A known antibiotic and antifungal compound produced by a soil microbe can inhibit another species of microbe from forming biofilms - -microbial mats that frequently are medically harmful -- without killing that microbe. (sciencedaily.com)

To evaluate in vitro the effect of a red propolis ethanolic extract (RPE) in the prevention of growth of a cariogenic biofilm and its cytotoxic potential. (bvsalud.org)

Either way, 60% of chronic wounds have a biofilm . (shieldhealthcare.com)
Thus, new approaches are required to combat biofilms in chronic wounds. (umf.org.nz)
Our results suggest that honey has potential as an adjunct treatment with rifampicin for chronic wounds infected with staphylococcal biofilms. (umf.org.nz)

Using the power of Microsoft's cloud and advanced machine learning, Princeton will be able to study different strains of biofilms in new ways to better understand how they work. (princeton.edu)
Red propolis extract showed antibacterial activity against the tested strains, exhibited acceptable cytotoxicity and reduced the colonization of S. mutans and L. casei in a biofilm membrane model. (bvsalud.org)

In the BacData project, MHH and L3S scientists will work together to understand the biology and dynamics of clinically relevant biofilms and to decipher their resistance mechanisms . (l3s.de)
But clinically diagnosing biofilm is difficult. (shieldhealthcare.com)

Recent evidence suggests that P. aeruginosa may actually grow as dense communities of exopolysaccharide-encapsulated biofilms adherent to CF patients' airways 2 - 4 . (ersjournals.com)
The purpose of the present study was to determine whether the PSB can sample directly from the airway biofilm and, thus, more accurately represent the state of P. aeruginosa in CF patients' lungs. (ersjournals.com)

Based on observations of dental plaque and sessile communities in mountain streams, Costerton et al. (cdc.gov)

Rea, Maria Angelica , Standish, Christopher , Shuster, Jeremiah , Bissett, Andrew and Reith, Frank (2018) Progressive biogeochemical transformation of placer gold particles drives compositional changes in associated biofilm communities. (soton.ac.uk)
Cite this: Survival of the Slimiest: The Persistent Nature of Biofilms - Medscape - Mar 22, 2018. (medscape.com)

Unsurprisingly, they found fungi (mold), as well as 'dense biofilms with complex bacterial and fungal communities. (discovermagazine.com)

This gives the slimy nature of the biofilm, and contains many sugars such as fructose, glucose, mannose, rhamnose, galactose and N-acetylglucosamine. (thepigsite.com)

Innate and induced resistance mechanisms of bacterial biofilms. (igem.org)

Natural biofilms grown on glass slides were exposed for 11 d to four controlled pCO2 concentrations representing the following scenarios: A) pre‐industrial (∼300 ppm), B) present‐day (∼400 ppm), C) mid century (∼560 ppm) and D) late century (∼1140 ppm). (leibniz-zmt.de)

We documented changes in microbial communities from seedstring stage throughout the growth and development of the hosts to provide a baseline understanding of temporal changes in the cultivated kelp microbiome. (wwu.edu)
however, the presence of candidal organisms within the overall biofilm lends credence to its role in the development and maintenance of denture stomatitis. (medscape.com)

In many older oil wells, much of the liquid extracted is water, which makes the insides of pipelines an ideal growth medium for biofilms. (harvard.edu)
In the later stages, when the majority of cells are sheltered from the flow by the surrounding extracellular matrix, buckling-induced cell verticalization in the biofilm core restores radially symmetric biofilm growth, in agreement with predictions from a 3D continuum model. (biorxiv.org)
Biofilms may provide a shield or sheltered microenvironment for the growth and/or colonization of Mycobacteria in MRFs. (cdc.gov)
Concrete biodeterioration and steel corrosion in the tunnel have been linked to the growth of iron- and manganese-oxidizing biofilms in areas of saline water seepage. (lu.se)

Multivariate statistical analyses showed a significant relationship between the extent of Au-particle transformation and biofilm community composition, with putative metal-resistant Au-cycling taxa linked to progressive Au transformation. (soton.ac.uk)
In conclusion, biogeochemical Au-cycling and Au-particle transformation occurred at all sites and exerted a strong influence on biofilm community composition. (soton.ac.uk)
This study investigates community composition and activity responses to experimental ocean acidification in biofilms from the Australian Great Barrier Reef. (leibniz-zmt.de)

The ESF workshop highlighted the huge potential and importance of biofilms, and also drew attention to exciting work unravelling the complex genetic and cellular interactions within these small yet teeming communities. (sciencedaily.com)
Microbial communities often display region-specific properties, which give rise to complex interactions and emergent behaviors that are critical to the homeostasis and stress response of the communities. (nature.com)
A biofilm may also be considered a hydrogel, which is a complex polymer that contains many times its dry weight in water. (wikipedia.org)

Anatomy2

Organisms15

Diseases3

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Psychiatry and Psychology1

Phenomena and Processes25

Disciplines and Occupations7

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture5

Information Science3

Named Groups1

Health Care26

Bacteria46

Microorganisms18

Different parts of the biofilm2

Antibiotics15

Surfaces13

Known as biofilms2

Infections11

Quorum5

Confocal laser scan1

Microbes5

Bacillus5

Formation25

Prevent biofilm3

Mycobacterium2

Multicellular communities2

Planktonic cultures1

Gene6

Genes4

Extracellular polymeric2

Nutrients3

Manuka honey2

Subtilis2

Antimicrobial2

Characterization1

Staphylococcus1

Comprises1

Proteins1

Denture wearers1

Pathogens2

Microbial community3

Bacterial communities3

Microorganism1

Catheters1

Microscopy3

Metabolic1

Form8

Surface9

Experiments2

Antibiotic1

Vitro1

Chronic wounds3

Strains2

Clinically2

Aeruginosa2

Sessile1

20182

Fungi1

Slimy1

Mechanisms1

Concentrations1

Development2

Growth4

Composition3

Complex3