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  • oral biofilms
  • Rickard AH, Campagna SR, Kolenbrander PE (2008) Autoinducer-2 is produced in saliva-fed flow conditions relevant to natural oral biofilms. (springer.com)
  • While the oral bacteria exhibit high tropism for their specialized ecological niche, it is not clear if bacteria that are not part of the normal oral microbiota can efficiently colonize and grow within oral biofilms. (uzh.ch)
  • pathogens
  • We found that essential parameters of cospecies biofilm maintenance and maturation, such as metabolic activity, biofilm thickness, roughness, extracellular polysaccharides production, and morphology of both pathogens, were altered by SRM-S-8 in the flow system. (hindawi.com)
  • We propose that prolonged and sustained release of S-8 in a flow-through system allows better penetration of the active agent to deeper layers of the mixed biofilm, thereby increasing its activity against both pathogens. (hindawi.com)
  • In plant pathology, the antimicrobial activities of chitosan compounds have been mainly evaluated on plant fungal pathogens [ 20 - 23 ], and few studies were done on chitosan action directed towards viruses and bacteria in plant [ 24 - 26 ]. (hindawi.com)
  • A hundred and seventeen tested isolates were identified into 23 genera, including plant related bacteria and coliforms with some opportunistic pathogens. (umd.edu)
  • Cruz Olivo EA, Santos D, Elena de Lima M, Dos Santos VL, Sinisterra RD, Cortes ME (2016) Antibacterial effect of synthetic peptide LyeTxI and LyeTxI/betaCD association compound against planktonic and multispecies biofilms of periodontal pathogens. (springer.com)
  • Sanchez MC, Marin MJ, Figuero E, Llama-Palacios A, Leon R, Blanc V, Herrera D, Sanz M (2014) Quantitative real-time PCR combined with propidium monoazide for the selective quantification of viable periodontal pathogens in an in vitro subgingival biofilm model. (springer.com)
  • diatoms and bacteria
  • phdthesis{Buhmann2015Inter-29511, title={Interactions between diatoms and bacteria in laboratory biofilm model communities}, year={2015}, author={Buhmann, Matthias Torben}, note={Die Dissertation ist 2015 erschienen. (uni-konstanz.de)
  • Defined co-cultures of diatoms and bacteria support this hypothesis by indicating that different bacteria utilize different fractions of secreted diatom carbohydrates. (uni-konstanz.de)
  • acute
  • Though antibiotics have proven to be effective for children with acute OM where biofilms have not yet formed, those with chronic disease typically benefit little from the drugs and more so from myringotomy, a surgical procedure in which small tubes are placed in the eardrum to continuously drain infectious fluid (called effusion). (innovations-report.com)
  • infection
  • In the case of bacteria on a prosthetic, biofilms protect the colony of bacteria from its environment, including from antibiotics a doctor might prescribe to attack the infection. (futurity.org)
  • continues and there can be a new spread of the bacteria and a new re-infection. (coursera.org)
  • The clinical diagnosis can be assisted by laboratory tests like white cell count and differentiation, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and others, but there is no gold standard, having a perfect sensitivity and specificity for diagnosing the infection, apart from identifying the infective bacteria ( 4 ). (frontiersin.org)
  • wound infection, especially biofilm infection, is one of these delaying factors. (cardiffmet.ac.uk)
  • quantitatively
  • Here we report a technique to estimate the proportion of the bacterial population on leaves that is assembled in biofilms and to quantitatively isolate bacteria from the biofilm and nonbiofilm (solitary) components of phyllosphere microbial communities. (asm.org)
  • Investigating diatom carbohydrates by HPAE-PAD and spectrophotometric assays revealed that diatom carbohydrate secretion is influenced predominately quantitatively by bacteria. (uni-konstanz.de)
  • By quantifying diatom protein secretion and separating diatom proteins via SDS-PAGE we found diatom protein secretion to be influenced qualitatively and quantitatively by bacteria. (uni-konstanz.de)
  • Microbes
  • Microbes form a biofilm in response to many factors, which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics. (scribd.com)
  • The described method is reproducible and of high resolution, and allows the examination of microbes' composition and viability within a biofilm structure. (springer.com)
  • Microbes that create troubling biofilms are colonized in water pipes, teeth, lung tissue, heart valves, and implants such as catheters and prosthetic hips. (umn.edu)
  • Through the partnership, they are exploring ways to change the mechanical properties of biofilms by manipulating the ecology of the films, which often contain multiple types of microbes. (umn.edu)
  • form
  • But now a new study, whose lead author is Eva Sapi, professor and head of biology and environmental science at the University of New Haven, suggests that the reason the Borrelia spirochetes that cause Lyme disease are resistant to treatment is because they form a biofilm in the body that allows it to "hide out" from antibiotics. (lymedisease.org)
  • Dr. Duraj-Thatte reviews the means that the bacteria she works with can be used for creating biofilms that can have rigid form and could be used to create synthetic biological building materials. (harvard.edu)
  • On any sort of device that you try to put in a human, a biofilm will form. (futurity.org)
  • When bacteria collect on a surface, they form a protective layer called a biofilm. (futurity.org)
  • On any sort of device that you try to put in a human, a biofilm will form," says Matthew Chapman, professor of molecular, cellular, and developmental biology at the University of Michigan. (futurity.org)
  • They will stick to this area surface and form a new biofilm. (coursera.org)
  • She added: "People need to question whether disinfectants that are promoted as killing various types of bacteria are really as effective in real life situations where biofilms can form as they are claimed to be based on experiments that do not use biofilms. (thepoultrysite.com)
  • Oral bacteria can spontaneously form biofilms on the surface of teeth, which may compromise the health of the teeth, or their surrounding (periodontal) tissues. (uzh.ch)
  • When biofilms form on surgical implants the recourse is to try to blast them with antibiotics- which have trouble breaking through-or to surgically remove the implant and put in a new one. (umn.edu)
  • Like biofilms in other environments, those in the phyllosphere are tens of micrometers thick, have copious exopolymeric matrices, and, in certain cases, form extensive networks several millimeters long. (asm.org)
  • Microbiology
  • The article is the first to demonstrate the presence of Borrelia biofilm in human infected skin tissues, confirming that these structures indeed can exist in the human body published in the European Journal of Microbiology and Immunology, an international, peer-reviewed online journal. (lymedisease.org)
  • The world biofilm was originally created in, in technical microbiology. (coursera.org)
  • Ray Hozalski, professor of civil engineering, and Gary Dunny, professor of microbiology in the University's Medical School, are working on ways to manipulate the strength of biofilms. (umn.edu)
  • proteins
  • The biofilm matrix is a huge reservoir that can contain tons of proteins, fused to any sort of antigen you are interested in," she says. (childrenshospital.org)
  • According to this patent, transformed bacteria are being used in modern biotechnology to produce recombinant proteins and various molecules for food, pharmaceutical and biocatalysis applications. (cosmeticsandtoiletries.com)
  • recombinant
  • Thus any study regarding the fate of recombinant DNA sequences in either an open environment or closed reactor system must consider processes that impact plasmid retention and expression in a biofilm culture. (montana.edu)
  • Using recombinant bacteria within a biofilm reactor to degrade a recalcitrant waste requires finding a suitable host to harbor and express the desired degrading plasmid phenotype. (montana.edu)
  • mutans
  • In the present study, we used a constant depth film fermenter model in order to investigate the impact of SRM-S-8 on mixed C. albicans - S. mutans biofilm development, under flow conditions. (hindawi.com)
  • S. mutans biofilms progress when bacteria utilize dietary sugars and proliferate on tooth surface, forming microcolonies that are firmly imbedded within EPS [ 3 ]. (hindawi.com)
  • confocal
  • To examine bacterial localization within biofilms, confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM) were used. (umd.edu)
  • populations
  • Based on problems associated with the use of chlorine as a water disinfectant and with the control of bacterial populations in biofilms, 1,3-dichloro-2,2,5,5-tetramethy1-4- imidazolidinone (compound DC) and 1-bromo-3-chloro-2,2,5,5- tetramethy1-4-imidazolidinone (compound DBC) were used to control planktonic and sessile populations of Klebsiella. (gatech.edu)
  • periodontal
  • Direct person-to-person transmission of periodontal bacteria through saliva has recently been widely reported and dental units have been demonstrated to retract saliva from patients under treatment and to release it into the mouths of subjects undergoing the next operation. (nih.gov)
  • otitis media
  • The discovery of biofilms in the setting of chronic otitis media represents a landmark evolution in the medical community's understanding about a disease that afflicts millions of children world-wide each year and further endorses the emerging biofilm paradigm of chronic infectious disease, said Garth Ehrlich, Ph.D., principal investigator and executive director of the ASRI Center for Genomic Sciences. (innovations-report.com)
  • Nearly all of the children in our study who suffered from chronic otitis media tested positive for biofilms in the middle ear, even those who were asymptomatic. (innovations-report.com)
  • and thirdly - why speculate about biofilms in otitis media? (coursera.org)
  • disinfectant
  • We found that it was not possible to kill the Salmonella cells using any of the three disinfectants, if the biofilm was allowed to grow for seven days before the disinfectant was applied," said Mary Corcoran, a researcher on the study. (thepoultrysite.com)
  • cells
  • Unlike the more familiar free-swimming bacterial forms, cells in biofilms surround themselves with a complex matrix, better known as 'slime,' to protect the cells from environmental stresses such as the attack of the immune system or the exposure to antibiotics. (lymedisease.org)
  • The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single-cells that may float or swim in a liquid medium. (scribd.com)
  • Results NaOCl (1%) affected the membrane integrity of all organisms and removed most biofilm cells. (mau.se)
  • Exposure to EDTA (50 mmol/L) affected the membrane integrity in all organisms but failed to remove more than a few cells in biofilms of E. faecalis, L. paracasei, and S. anginosus. (mau.se)
  • Conclusions The biofilm system developed here was sensitive and differences in cell membrane integrity and removal of biofilm cells after exposure to antimicrobials commonly used in endodontics was discernible. (mau.se)
  • Once the cells "sense" that a quorum has been reached, they begin to communicate, a process that in turn "throws the switch" for manufacturing virulence factors, such as biofilms. (scienceblog.com)
  • Underneath the protective biofilm, the cells are happily reproducing, damaging the tissue and producing toxins," said Suga. (scienceblog.com)
  • Swimming motility by means of the single polar flagellum enhances initial attachment and enables progeny swarmer cells to escape from the monolayer biofilm. (stanford.edu)
  • These unique biofilm features demonstrate a spatial diversification of the C. crescentus population into a sessile, "stem cell"-like subpopulation (monolayer biofilm), which generates progeny cells capable of exploring the aqueous, oligotrophic environment by swimming motility and a subpopulation accumulating in large mushroom structures. (stanford.edu)
  • catheters
  • One of the most important health implications for biofilms is on catheters. (futurity.org)
  • In addition to cystic fibrosis patients, whose lungs become clogged with the bacteria, it infects patients receiving chemotherapy, burn patients, AIDS patients, those on ventilators, with catheters and others. (scienceblog.com)
  • susceptible
  • After a lag time the bacteria derived from biofilms became susceptible to vancomycin and clindamycin, provided that the medium was refreshed. (lu.se)
  • protective
  • These tough, layered, polysaccharide shells provide the bacteria with a nearly impenetrable, self-protective mechanism that makes it extremely difficult, and in some cases impossible, to fight with antibiotics. (scienceblog.com)
  • growth
  • ATR spectroscopy of −80°C planktonic and biofilm growth showed variation in absorption spectra in fingerprint (1200−900 cm −1 ) region. (hindawi.com)
  • When a cell switches to the biofilm mode of growth, it undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated. (scribd.com)
  • Wang H, Dong Y, Wang G, Xu X, Zhou G (2016) Effect of growth media on gene expression levels in Salmonella Typhimurium biofilm formed on stainless steel surface. (springer.com)
  • Therefore, a sterile incubation chamber for the growth of bacteria-free biofilms, and defined diatom/bacteria co-cultures was developed. (uni-konstanz.de)
  • Bacteria were found to influence diatom growth strongly, either direct or by constitutively released soluble substances. (uni-konstanz.de)
  • We found concentrations of dissolved free amino acids (DFAA), analyzed by HPLC via ortho-phthaldialdehyde derivatization, to correlate with diatom growth when influenced by bacteria, and thus postulate that DFAA may be one class of such substances, regulating growth rate and growth density of diatoms. (uni-konstanz.de)
  • substrate
  • In addition, the biofilm architecture and the extent to which coating of the substrate surface with collagen influenced the resistance pattern to the chemicals were also analyzed. (mau.se)
  • Hence, aquatic biofilms can be isolated simply by removing a portion of the substrate from the system ( 5-7 , 11 , 14 , 16 ). (asm.org)
  • Furthermore, where viable plate counts or immunological techniques are used to assess the population size of the bacteria in biofilms, mechanical detachment of the biofilms from the substrate also serves to disintegrate the biofilms ( 1 , 6 , 7 , 11 , 14 , 16 ). (asm.org)
  • Hence, the size of the biofilm component in these systems can also be assessed via protein assays or biomass determination without the need to detach the biofilms from the substrate ( 5 ). (asm.org)
  • tissues
  • Since then, the team has focused on actually finding the biofilm in human tissues. (lymedisease.org)
  • The presence of microbial biofilms in the phyllosphere of terrestrial plants has recently been demonstrated, but few techniques to study biofilms associated with living plant tissues are available. (asm.org)
  • cultures
  • Two clear absorption bands were prominent in biofilm at 1175 and 1143 cm −1 whereas, one prominent broad band at 1075 cm −1 resulting from the overlapping of two band was noted in planktonic cultures. (hindawi.com)
  • Among the bacteria already widely used for industrial purposes are the lactic acid bacteria, which are employed as starter cultures. (cosmeticsandtoiletries.com)
  • This paper reports on a laboratory evaluation of factors governing plasmid retention and the expression of TCE degradative capacity in both suspended and biofilm cultures. (montana.edu)
  • Ecology
  • The occurrence of such long-distance electron transport by cable bacteria challenges conventional ideas about electron transport across redox gradients and thus could have profound implications for our understanding of the microbial ecology and biogeochemistry of marine sediments. (asm.org)
  • tough
  • When the hygienist begins scraping your teeth, he or she is removing a biofilm-a tough, living tissue made up of one or more types of microbe. (umn.edu)