StructuralCryo-electron mMass spectrometryChemistryElectron microscopyBiologyMegahertz serial crystallographySerial Femtosecond CrystallographyCryoEMMacromolecularNeutron crystallographyProteinsFacultyBiochemicalLund UniversityMedical BiochemistryNuclearLigandDecipherDirector at the Max Planck InstiExperimentsMaster'sSpectrometryEXAFSComputationalKinaseDiffractionTraditionallyLaboratoryCrystalsFELsResearchSynchrotronStructurePrinciples
Structural22
- This method describes the cloning, expression, and purification of recombinant Nsa1 for structural determination by X-ray crystallography and small-angle X-ray scattering (SAXS), and is applicable for the hybrid structural analysis of other proteins containing both ordered and disordered domains. (jove.com)
- The overall goal of this procedure is to produce a recombinant protein that is composed of a mixture of ordered and disordered domains for hybrid structural analysis by both x-ray crystallography and small angle x-ray scattering. (jove.com)
- The purpose of the X-ray Crystallography Shared Service is to provide equipment, training, assistance, and technological innovations for determining three-dimensional structures of protein and other macromolecules to understand the structural basis for biological function and dynamics. (umms.org)
- Structural characterization of PvdA and PvdF by x-ray crystallography is underway. (ku.edu)
- Basic principles of modern biophysical and structural methods used to interrogate macromolecules from the atomic to cellular levels, including light and electron microscopy, X-ray crystallography, NMR spectroscopy, single molecule techniques, circular dichroism, surface plasmon resonance, mass spectrometry, and molecular dynamics and systems biological simulations. (caltech.edu)
- We use a multidisciplinary approach integrating data & computational science, cell biology, molecular biology, microbiology, biochemistry, glycobiology, immunology, structural biology (cryo-EM and X-ray crystallography), and murine models to decipher the interactions between the host and (drug-resistant) bacterial pathogens. (google.com)
- By the time he was in a PhD program at Berkeley, he'd dived deep into biochemistry and structural biology, studying how ribosomes and other higher-order assemblies take shape. (rockefeller.edu)
- Valerie Khayyo, a Stony Brook graduate student in the Biochemistry & Structural Biology Program and first author of the study, added that the structure enables researchers to understand and see specific mutational changes in the amino acid building blocks of lipins that result in disease. (sciencedaily.com)
- The Perelman School of Medicine (PSOM) has announced the establishment of the Penn Institute for Structural Biology (ISB) , which will be led by Dr. Vera Moiseenkova-Bell , Professor in the Department of Systems Pharmacology and Translational Therapeutics and Secondary faculty in the Department of Biochemistry and Biophysics. (upenn.edu)
- The ISB will catalyze novel advances in the areas of structural biology, provide access to expertise and technology to support cryo-EM, Xray crystallography, NMR, molecular dynamics simulations and more, and promote collaborative studies across the biomedical community. (upenn.edu)
- In such structural studies, Beese uses X-ray crystallography, a technique that involves shining X-rays through a crystallized protein and deducing the molecule?s structure from the resulting pattern of diffracted spots. (scienceblog.com)
- Our laboratory studies molecular mechanisms of the host machinery mediating defense against invading pathogens using a structural biology approach that integrates cryo-EM imaging with methods from biophysics, protein biochemistry and cell biology. (academictransfer.com)
- We employ a multidisciplinary approach including kinase biochemistry and biology, structural biology (X-ray crystallography and cryo-electron microscopy), assay development, high-throughput screening, proteomics, imaging and chemical biology to gain high-resolution insights into kinase/pseudokinase signaling proteins. (edu.au)
- I joined Genentech at the end of 2017 following postdoctoral work in structural biology and biochemistry at Harvard Medical School, and a PhD at Vanderbilt University. (gene.com)
- Using the latest techniques in protein biochemistry, high resolution structural biology, x-ray crystallography and cryoEM, we study macromolecular complexes across widely differing scales and functions, such as E3 ubiquitin ligases, nucleosomes and the kinetochore. (gene.com)
- The Biochemistry, Quantitative Biology, Biophysics and Structural Biology (BQBS) Track provides students with experimental, theoretical, and computational research training across a broad range of scales, from molecules to cells to whole organisms. (yale.edu)
- Our approaches encompass a full range of modern disciplines including biochemistry and molecular biology, biophysics, chemistry, cell biology, genomics and proteomics, computational biology, and structural biology. (yale.edu)
- Neutron crystallography is emerging as a unique and highly promising technique for structural biology because, unlike other techniques, it can probe the locations of protons (or more specifically the heavier isotope deuterium) which are paramount for understanding biochemical processes, e.g. the hydrogen-bond pattern, the charge and tautomeric states, as well as the interactions with the surrounding solvent. (lu.se)
- NMR, cryoEM and X-ray crystallography), its success hinges on the development of sophisticated software that converts the raw detector data to structural information. (lu.se)
- Through combining cryo-electron microscopy at different pHs, X-ray crystallography and biochemical and structural analysis, we showed that NendoU can shift between open and closed forms, which probably correspond to active and inactive states, respectively. (lu.se)
- The Center for Molecular Protein Science (CMPS) brings together scientists active within the fields of biochemistry, molecular biophysics, structural biology, and physical and theoretical chemistry. (lu.se)
- CMPS is a highly integrated center comprising the divisions of Biochemistry and Structural Biology (Faculty of Science) and Biophysical Chemistry (Faculty of Engineering, LTH). (lu.se)
Cryo-electron m3
- Technical advances in X-ray crystallography, cryo-electron microscopy (cryo-EM), live imaging, single molecule studies, next-generation sequencing, and mass spectrometry have led to a wealth of quantitative data for addressing long-standing biology questions. (yale.edu)
- He also performs unique dermatologic research studying the three-dimensional structures of skin-related proteins using x-ray crystallography and cryo-electron microscopy. (yale.edu)
- He performs laboratory research with the goal of using x-ray crystallography, cryo-electron microscopy, and biochemistry to better understand the structure-function relationship of proteins involved in normal and diseased skin and dermatologic therapeutics. (yale.edu)
Mass spectrometry3
- We primarily use X-ray crystallography but also work with biochemistry, microbiology, mass spectrometry and electron microscopy. (caltech.edu)
- The team used X-ray crystallography, mass spectrometry and biochemistry to visualize the structure, which represents the active state of a lipin enzyme during the production of triglycerides, as well as its other functions namely lipoprotein assembly and cellular signaling. (sciencedaily.com)
- Develop high-level skills in nuclear magnetic resonance spectroscopy, x-ray crystallography, mass spectrometry, chromatography, atomic absorption and other advanced techniques. (unco.edu)
Chemistry6
- Dr. Suits earned earned his Bachelor of Science Education in Secondary Education in Chemistry and Physics at New Mexico State University, followed by a master's in Biochemistry at Southwest Texas State University and a doctorate in Science Education from the University of Texas, Austin. (unco.edu)
- The candidate should have a degree in biology, biochemistry, chemistry, physics or a related field, with research experience in molecular biology and enthusiasm for working at the interface of biology, chemistry, and physics. (academictransfer.com)
- Experimental work, being a central topic in any research in biochemistry and biophysical chemistry, includes laboratory practicals where the students learn planning, running, documenting and analyzing experiments. (lu.se)
- Biochemistry , Synthetic and Analytical Chemistry and Physical Chemistry international master's programs. (lu.se)
- The technique therefore significantly impacts many life-science areas, such as biochemistry, biotechnology and medicinal chemistry. (lu.se)
- CMPS offers undergraduate and Master's level courses in biochemistry and biochemical techniques, as well as various courses in biophysical chemistry, NMR spectroscopy, and general physical chemistry. (lu.se)
Electron microscopy5
- however, they are increasingly merging these methods with other experimental tools such as small-angle X-ray scattering, single-molecule approaches, and electron microscopy. (hopkinsmedicine.org)
- You will find out about the experimental techniques, such as X-ray crystallography, nuclear magnetic resonance and electron microscopy, which are used to determine the 3D structure of proteins. (manchester.ac.uk)
- Outline methods for 3D structure determination by X-ray crystallography, NMR and electron microscopy. (manchester.ac.uk)
- The Department of Bionanoscience will provide outstanding infrastructure for nanofabrication, fluorescence microscopy molecular/cell biology, biochemistry, state-of-the-art electron microscopy and high-performance computing for image processing. (academictransfer.com)
- Previous experience with electron microscopy or X-ray crystallography is beneficial, but not required. (academictransfer.com)
Biology13
- Led by Mike Airola, PhD, of the Department of Biochemistry and Cell Biology in the College of Arts and Sciences and Renaissance School of Medicine at Stony Brook University, the structure also provides scientists with insights as to why mutations in the enzyme cause a loss of activity that leads to abnormal production of triglycerides implicated in heart disease, obesity and diabetes. (sciencedaily.com)
- This structure answers a long-standing question for how two essential regions, N-lip and C-lip, which are located on opposite ends of this protein in humans, come together to form a function enzyme to help make triglycerides," explains Airola, Assistant Professor in the Department of Biochemistry & Cell Biology and director of the Airola Lab. (sciencedaily.com)
- George Burslem is an Assistant Professor in the Departments of Biochemistry and Biophysics, and Cancer Biology. (upenn.edu)
- Training will be provided in molecular biology, protein biochemistry, cryo-EM and associated image-processing techniques as well as X-ray crystallography. (academictransfer.com)
- Our Biochemistry degree is recognised by the Royal Society of Biology (RSB). (kent.ac.uk)
- In your first year, your modules give you an insight into various biological and chemical disciplines, including biochemistry, cell and molecular biology, microbiology and physiology. (kent.ac.uk)
- We use cultured mammalian cells, Xenopus oocytes and zebrafish as model systems, and study structure and function of ion channels and receptors with a combined molecular biology, biochemistry, biophysics, x-ray crystallography, and electrophysiology approach. (stjohns.edu)
- The book is rewarding because it assembles contributions from diverse fields such as X-ray crystallography, electrophysiology, biochemistry, cell biology and developmental biology. (iospress.com)
- Combining X-ray crystallography data from beamline ID30B and biochemistry with cell biology reveals how the conserved signal transduction machinery in bacteria and archaea is coupled to the archaeal rotary motor termed the archaellum. (esrf.fr)
- With research ranging from theoretical biology to RNA biochemistry, BQBS is a wonderful place to study for folks interested in a wide array of topics. (yale.edu)
- Thanks to pioneers like Max Delbrück, who crossed over from physics to biology in the middle of the 20th century, the influence of quantitative analyses from the physical sciences helped to give rise to mechanistic, molecular approaches in cell biology and biochemistry that led to many revolutionary discoveries. (scienceandnonduality.com)
- Our research focuses on major areas of modern biochemistry and molecular biology. (lu.se)
- CoLuAa is this year organized by the Biochemistry and Structure Biology department at Lund University and the MAX IV Laboratory . (lu.se)
Megahertz serial crystallography1
- The results indicate the feasibility for megahertz serial crystallography measurements with hard X-rays and give guidance for the design of such experiments. (iucr.org)
Serial Femtosecond Crystallography3
- Serial femtosecond crystallography (SFX) is a form of X-ray crystallography developed for use at X-ray free-electron lasers (XFELs). (wikipedia.org)
- Liquid microjets are a common means of delivering protein crystals to the focus of X-ray free-electron lasers (FELs) for serial femtosecond crystallography measurements. (iucr.org)
- At the European XFEL, up to 27 000 pulses s −1 will be delivered to an experimental endstation, which is over 200 times more than the 120 Hz of the Linac Coherent Light Source (LCLS), where many serial femtosecond crystallography experiments have been conducted. (iucr.org)
CryoEM1
- single-particle cryoEM and X-ray crystallography. (lu.se)
Macromolecular8
- The facility provides instrumentation and expertise for collecting and processing x-ray diffraction data from macromolecular single crystals and is available to all the research groups inside and outside the campus. (umms.org)
- Specialist in Research 1991-1998 Macromolecular Crystallography, Massachusetts Institute of Technology. (uvm.edu)
- HYDERABAD, India - August 21st, 2017 - At the 24th Congress & General Assembly of the International Union of Crystallography (IUCr), Bruker today announces the new, high-performance D8 VENTURE BIOTOOLS TM for laboratory macromolecular crystallography. (bruker.com)
- Dr. Vernon Smith, the Business Development Manager for Macromolecular Crystallography at Bruker AXS, noted: "With the D8 VENTURE BIOTOOLS , Bruker has once again set new standards in crystallography systems for the home laboratory. (bruker.com)
- The purpose is to make neutron macromolecular crystallography at ESS world leading and available to the biotechnological and pharmaceutical industry in Sweden. (lu.se)
- However, neutron macromolecular crystallography is still in its infancy with "only" 170 of the total 200 000 structures in the Protein Data Bank determined by this technique. (lu.se)
- Moreover, the software required for getting from raw data to scientific results is inadequate and often tedious to work with, which is a major hindrance for the wider use of neutron macromolecular crystallography, particular for the life science community that does not have a tradition of developing software. (lu.se)
- It is this latter aspect that is addressed in this proposal for the NMX (neutron macromolecular crystallography) instrument at ESS, which, combined with the high flux at ESS, will make NMX a truly world leading instrument attractive for industrial and academic research. (lu.se)
Neutron crystallography1
- Principles of X-ray crystallography, neutron crystallography, small angle X-ray and neutron scattering. (lu.se)
Proteins4
- Many of the skills first encountered in the Proteins unit are reinforced in the Biochemistry/Medical Biochemistry semester 4 tutorial program. (manchester.ac.uk)
- But trying to capture the conformations of proteins using X-ray crystallography, which was the standard methodology back then, frustrated him. (rockefeller.edu)
- Dr. Liu's research group primarily employs X-ray crystallography in combination with mechanistic, biological and computational methods to study proteins involved in bacterial infections and other human diseases. (luc.edu)
- The main aim of the course is to enable students to acquire specialised knowledge and understanding of membrane biochemistry and the molecular structure, topology and functional mechanisms of membrane proteins. (lu.se)
Faculty2
- The course focuses on a seminar on selected topics from outside faculty on recent advances in biochemistry. (caltech.edu)
- Components for each faculty visit include participation in a recitation, a formal discussion section with visiting faculty, and attendance of the Biochemistry seminar. (caltech.edu)
Biochemical1
- The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)
Lund University1
- Ulf Ryde was born in 1963 and obtained a PhD in biochemistry methods and their possible combination with quantum chemical (enzyme kinetics and control theory) at Lund University, Sweden, methods, including a number of typical applications. (lu.se)
Medical Biochemistry1
- Russell's scientific background includes a degree in medical biochemistry from the University of Glasgow and a PhD relating to the determination of the protein structure of glutathione-S-transferases to enhance herbicide selectivity. (murgitroyd.com)
Nuclear2
- X-ray absorption spectroscopy to analyze nuclear geometry and electronic structure of biological metal centers-potential and questions examined with special focus on the tetra-nuclear manganese complex of oxygenic photosynthesis. (fu-berlin.de)
- Using both X-ray crystallography and nuclear magnetic resonance spectroscopy, UCLA biochemists have examined the structure of p65 protein and its interaction with telomerase RNA. (scitechdaily.com)
Ligand2
- We forge and wield new tools in the field of X-Ray Crystallography to decipher the molecular basis of natural and exogenous ligand binding, and the propagation of conformational change. (uvm.edu)
- By fragment screening using x-ray crystallography we identified four ligands revealing ligand-binding sites in conserved interfaces between SARS-CoV-2 nsp10 and nsp14/nsp16. (lu.se)
Decipher1
- Using X-ray crystallography, the scientists could already decipher the structure of a subunit of the IFT complex, and others shall follow. (mpg.de)
Director at the Max Planck Insti1
- The Leopoldina honors Franz-Ulrich Hartl, Director at the Max Planck Institute of Biochemistry, with the Schleiden Medal for his contributions to the understanding of cellular protein folding. (mpg.de)
Experiments3
- This method has since been extended to solve unknown structures, perform time-resolved experiments, and later even brought back to synchrotron X-ray sources. (wikipedia.org)
- Photosynthetic O2 formation tracked by time-resolved x-ray experiments. (fu-berlin.de)
- Perform X-ray crystallography experiments at a basic level, from which the participant can further develop the skills on his/her own. (lu.se)
Master's1
- We also host the Master's program in Biochemistry . (lu.se)
Spectrometry1
- Facilities include those for protein-sequence analysis, peptide and oligonucleotide synthesis, access to the synchrotron at Lawrence Berkeley National Lab for X-ray crystallography, and NMR spectrometry. (berkeley.edu)
EXAFS1
- metal site is extended X-ray absorption fine structure (EXAFS). (lu.se)
Computational1
- This will be pursued by the development of sophisticated analysis software and by integrating methods from a Swedish expert research group in computational methods for crystallography into the pipeline. (lu.se)
Kinase1
- Catalytic mechanism of nucleoside diphosphate kinase investigated using nucleotide analogues, viscosity effects, and X-ray crystallography. (expasy.org)
Diffraction2
- Furthermore, this article provides optical images of X-ray-induced explosions together with Bragg diffraction from protein microcrystals exposed to trains of X-ray pulses repeating at rates of up to 4.5 MHz. (iucr.org)
- Focusing an X-ray FEL pulse onto such a sample leads to its complete destruction, which is why only one diffraction pattern can be collected per microcrystal. (iucr.org)
Traditionally1
- CoLuAa is a meeting traditionally organised by the protein crystallography groups in Co penhagen, Lu nd and Aa rhus (hence the name) but it is open to anyone interested. (lu.se)
Laboratory3
- Department of Biochemistry, X-ray Crystallography Core Laboratory, The University of Texas Health Science Center San Antonio, San Antonio, TX-78229, USA. (rcsb.org)
- The latest version of the Excillum METALJET TM , the brightest laboratory X-ray source today, is a powerful alternative to the compact, microfocus IµS DIAMOND . (bruker.com)
- With no gaps and no charge sharing, the PHOTON III detector delivers the ultimate in laboratory crystallography data quality. (bruker.com)
Crystals2
- This method of data collection is referred to as serial, referencing a row of crystals streaming across the X-ray beam, one at a time. (wikipedia.org)
- The reason for this is the limited flux at neutron sources, which therefore require very long exposure times (weeks) and very large crystals compared to X-ray crystallography. (lu.se)
FELs1
- With the advent of X-ray FELs with megahertz rates, the typical velocities of these jets must be increased significantly in order to replenish the damaged material in time for the subsequent measurement with the next X-ray pulse. (iucr.org)
Research1
- Discussion of research fields in biochemistry and molecular biophysics at Caltech. (caltech.edu)
Synchrotron1
- Professor Elena Conti, Director at the Max-Planck-Institute for Biochemistry in Martinsried, Germany, stated: "The D8 VENTURE with METALJET TM has enabled my group to collect quality data from even our most difficult projects and has made our synchrotron trips more productive. (bruker.com)
Structure2
- Here, we present the X-ray structure of the Nep1 homolog from the archaebacterium Methanocaldococcus jannaschii in its free form (2.2 A resolution) and bound to the S-adenosylmethionine analog S-adenosylhomocysteine (SAH, 2.15 A resolution) and the antibiotic and general methyltransferase inhibitor sinefungin (2.25 A resolution). (rcsb.org)
- She won the Noble Prize for Biochemistry in 1964 thanks to developing protein crystallography, also called x-ray crystallography, which allows us to see the molecular structure of a crystal. (simplek12.com)
Principles1
- The course deals with both the principles determining these properties and the methods, mainly X-ray crystallography, that is used to study them in modern molecular protein science. (lu.se)