• Our methods focus on protein crystallography, biochemical and biophysical characterization, in silico modeling, and drug design. (pewtrusts.org)
  • Junliang Sun (Peking University, Beijing, China) works mainly on the development of structure characterization methods (electron crystallography and powder X-ray diffraction) and the synthesis of inorganic materials. (iucr.org)
  • Methanosarcina mazei casposon organization and initial biochemical characterization. (elifesciences.org)
  • During the last two PostDocs the interest shifted from cell biology and biochemical characterization of transport complexes to more structural aspects by means of X-ray crystallography, which has been the major scientific focus for the last 16 years. (dkfz.de)
  • Biochemical characterization of PvdA reveals that PvdA is specific for the coenzymes, FAD and NADPH, as well as for the substrate, L-ornithine. (ku.edu)
  • Structural characterization of PvdA and PvdF by x-ray crystallography is underway. (ku.edu)
  • We use state-of-the-art facilities in X-ray crystallography, NMR spectroscopy, mass spectrometry, cryo-electron microscopy, imaging, biophysical and biochemical analysis, molecular and cell biology. (ucl.ac.uk)
  • To address these challenges, we use X-ray crystallography and cryo-electron microscopy (cryo-EM) in combination with biophysical, biochemical, and cell biological methods. (psi.ch)
  • Through combining cryo-electron microscopy at different pHs, X-ray crystallography and biochemical and structural analysis, we showed that NendoU can shift between open and closed forms, which probably correspond to active and inactive states, respectively. (lu.se)
  • We apply a combination of the structural (including protein crystallography, small angle x-ray scattering, cryo-electron microscopy (Cryo-EM) etc.), biocomputational and biochemical techniques to study the ubiquitylation and deubiquitination processes, and recognition of the ubiquitin chains by the proteins harboring ubiquitin-binding domains. (stanford.edu)
  • A team has used neutron crystallography to image all of the atoms in a radical intermediate of a copper amine oxidase enzyme. (sciencedaily.com)
  • Now, in a study recently published in ACS Catalysis , a team led by researchers at Osaka Medical and Pharmaceutical University and Osaka University has used neutron crystallography to image the atom-by-atom structure of a copper amine oxidase enzyme. (sciencedaily.com)
  • Neutron crystallography, which analyzes diffraction from atomic nuclei in the enzyme (all atoms have an atomic nucleus), is an alternative imaging technique that the researchers chose for their work. (sciencedaily.com)
  • Neutron crystallography is well-suited for answering these questions. (sciencedaily.com)
  • Neutron crystallography is emerging as a unique and highly promising technique for structural biology because, unlike other techniques, it can probe the locations of protons (or more specifically the heavier isotope deuterium) which are paramount for understanding biochemical processes, e.g. the hydrogen-bond pattern, the charge and tautomeric states, as well as the interactions with the surrounding solvent. (lu.se)
  • The techniques encompassed cloning, protein expression and purification, protein / DNA interaction studies, crystallisation and crystallography using previous experiences of the host-laboratory, as well as the research profile of the beneficiary. (europa.eu)
  • Today, there is an evolving need for more imaging methods and for biophysics that cannot be addressed with crystallisation and crystallography. (infn.it)
  • With the rapid progress and improvements in the fields of macromolecular crystallography, Cryo-EM, small angle scattering, electron diffraction and use of XFELs, the new LINXS theme INTEGRATIVE STRUCTURAL BIOLOGY is formed to advance cutting-edge research and to encourage new users to utilize integrative structure biology to address key scientific questions. (lu.se)
  • The first symposium, which is the kick-off event for the new LINXS theme, will focus on advanced, cutting-edge research in the fields of macromolecular crystallography, Cryo-EM, small angle scattering, electron diffraction, mass-spectroscopy, NMR, and use of XFELs etc. (lu.se)
  • The purpose is to make neutron macromolecular crystallography at ESS world leading and available to the biotechnological and pharmaceutical industry in Sweden. (lu.se)
  • However, neutron macromolecular crystallography is still in its infancy with "only" 170 of the total 200 000 structures in the Protein Data Bank determined by this technique. (lu.se)
  • Moreover, the software required for getting from raw data to scientific results is inadequate and often tedious to work with, which is a major hindrance for the wider use of neutron macromolecular crystallography, particular for the life science community that does not have a tradition of developing software. (lu.se)
  • It is this latter aspect that is addressed in this proposal for the NMX (neutron macromolecular crystallography) instrument at ESS, which, combined with the high flux at ESS, will make NMX a truly world leading instrument attractive for industrial and academic research. (lu.se)
  • The approach relies on a blend of structural, biochemical, and biophysical methods to define the architecture, function, evolution, and regulation of protein/nucleic acid complexes. (hopkinsmedicine.org)
  • To do so, we use X-ray crystallography, electron cryo-microscopy (cryo-EM) together with biochemical techniques to characterize the molecular mechanism of GPCR signalling complexes. (psi.ch)
  • Furthermore, the role of DNA thermodynamic "signatures" in lesion recognition by DNA repair enzymes, such as DNA glycosylases, is poorly understood, again because such enzymes catalyze the repair of these lesions, precluding their biochemical/structural study in corresponding protein/DNA complexes. (hhs.gov)
  • In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)
  • Lavoie & retained in many speciĀ®c complexes (for example, Carey, 1994), crystallography (Lawson & Sigler, Otwinowsk i et al. (lu.se)
  • CMPS offers undergraduate and Master's level courses in biochemistry and biochemical techniques, as well as various courses in biophysical chemistry, NMR spectroscopy, and general physical chemistry. (lu.se)
  • We offer in-depth coursework and diverse research opportunities that focus on understanding the biochemical mechanisms of life's critical molecular processes. (rochester.edu)
  • His laboratory uses x-ray crystallography to determine the three-dimensional structures of proteins involved in signaling, as well as biochemical, biophysical, and cell biological analyses to elucidate mechanisms. (amgen.com)
  • She then completed her postdoctoral training at the MIT Department of Biology, where she used X-ray crystallography and biochemical techniques to study mechanisms of protein assembly in bacteria and human metabolic systems. (vanderbilt.edu)
  • These properties will then be compared to biochemical data, such as equilibrium binding constants to DNA glycosylases AAG and AlkD, and will be utilized as structural probes to gain insight regarding recognition and excision mechanisms employed by these enzymes. (hhs.gov)
  • In this project we will use a combined approach using computer-based methods including virtual screening of molecular databases and biochemical/biophysical techniques as well as X-ray crystallography to develop cofactor-specific inhibitors. (uni-wuerzburg.de)
  • For in vitro characterizations, we utilize X-ray crystallography, NMR spectroscopy, Cryo-EM and a wide variety of biophysical, biochemical and cellular techniques and methods (i.e. (leloir.org.ar)
  • Her studies of the molecular processes of protein transport within the cell are based on biochemical research methods combined with protein crystallography. (uni-heidelberg.de)
  • Following the doctrine of "Structure Determines Function", our research evolves around determining key protein structures that are mechanistically informative on biochemical processes. (luc.edu)
  • The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)
  • Cryoelectron microscopy and x-ray crystallography analyses of NoV VLPs identified the shell (S) and protruding domains (subdomains P1-1, P1-2, and P2) ( 18 ). (cdc.gov)
  • His main research goal has been to apply electron diffraction to structural problems in any field of crystallography. (iucr.org)
  • His work is largely based on diffraction phenomena and combines computational, biochemical and biophysical experiments. (iucr.org)
  • Chemical and biochemical techniques used to purify and determine the structures of biomolecules. (bath.ac.uk)
  • Meanwhile, Slade, an expert in protein crystallography, will determine X-ray crystal structures of IspD, the target enzyme of these anti-malarial compounds. (newswise.com)
  • She solved the structures of important biochemical substances including penicillin and vitamin B-12, for which she received the Nobel Prize in 1964. (byu.edu)
  • The use of molecular mechanics calculations to supplement experimental data in standard X-ray crystallography and NMR refinements is discussed and it is shown that structures can be locally improved by the use of quantum chemical calculations. (lu.se)
  • Protein structures are invaluable for biochemical studies and for mechanical or molecular mechanics calculations have become the understanding of how proteins work. (lu.se)
  • Dr. Liu's research group primarily employs X-ray crystallography in combination with mechanistic, biological and computational methods to study proteins involved in bacterial infections and other human diseases. (luc.edu)
  • Therefore, having a reliable cascade of screening methods to be used as pre-screens prior to labor-intensive X-ray crystallography would be extremely beneficial. (uni-marburg.de)
  • 1988). This observation stimulated further dence of the A to B-DNA conversion (Franklin & studies by biochemical and genetic methods (for Gosling, 1953). (lu.se)
  • This will be pursued by the development of sophisticated analysis software and by integrating methods from a Swedish expert research group in computational methods for crystallography into the pipeline. (lu.se)
  • A computer exercise in bioinformatics and a laboratory task for several days that includes experimental design and common biochemical methods including protein purification, affinity chromatography, electrophoresis and activity analysis with fluorometrics. (lu.se)
  • Here, I present results from different biological projects where SANS has played a crucial role by providing unique restraints for structural refinement and interpretation, complementary to other techniques (NMR, EM, crystallography). (lu.se)
  • BioSAXS is particularly powerful when combined with additional information from other biochemical and biophysical experiments such as crystallography. (stanford.edu)
  • This work has provided previously undisclosed structural details in a copper amine oxidase enzyme that has many functions in biochemical metabolism. (sciencedaily.com)
  • Five new Co-editors will work with Professor Zou - Mauro Gemmi, Louisa Meshi, Peter Nellist, Jose Rodriguez and Junliang Sun - and will aim to attract articles reporting important electron crystallography results and breakthroughs to the journals. (iucr.org)
  • There are pH-dependence, conformational change, and radical intermediate stabilization questions of our enzyme that X-ray crystallography in itself cannot fully explain," explains Takeshi Murakawa, lead author of the study. (sciencedaily.com)
  • They used bioinformatics and biochemical studies to show that this enzyme is functionally similar to the human version, so their results can be generalized to the human version, as well as the bacterial version. (sciencedaily.com)
  • Traditionally, X-ray crystallography was dominating the interaction of large facilities with life sciences. (infn.it)
  • The identification of initial lead conditions for successful protein crystallization is crucial for structural studies using X-ray crystallography. (uni-koeln.de)
  • I propose preparation and incorporation of methylated ribonucleotides 3-methyladenosine (3mA) and 7- methylguanosine (7mG, in place of their "normal", deoxyribose counterparts) into duplex DNA, as analogues with stabilized glycosidic bonds for biophysical, biochemical, and structural studies. (hhs.gov)
  • Electrophoretic mobility shifts will be used to determine binding constants, and X-ray crystallography will be used for structural studies. (hhs.gov)
  • p. v-vii (Advances in Biochemical Engineering/Biotechnology). (lu.se)
  • Identifying the exact positions of the smallest hydrogen atoms in these enzymes is challenging with commonly used technologies, but is critical to engineering improved enzymes that exhibit unusual yet useful biochemical reactivity. (sciencedaily.com)
  • The ISB will catalyze novel advances in the areas of structural biology, provide access to expertise and technology to support cryo-EM, Xray crystallography, NMR, molecular dynamics simulations and more, and promote collaborative studies across the biomedical community. (upenn.edu)
  • This method describes the cloning, expression, and purification of recombinant Nsa1 for structural determination by X-ray crystallography and small-angle X-ray scattering (SAXS), and is applicable for the hybrid structural analysis of other proteins containing both ordered and disordered domains. (jove.com)
  • An extensive high- and low-resolution structural analysis of Deinococcus radiodurans RecN using a combination of protein crystallography and small-angle X-ray scattering enabled us to assemble a quasi-atomic model of the entire RecN protein, representing the complete structure of a SMC-like protein. (rcsb.org)
  • The overall goal of this procedure is to produce a recombinant protein that is composed of a mixture of ordered and disordered domains for hybrid structural analysis by both x-ray crystallography and small angle x-ray scattering. (jove.com)
  • Using X-ray crystallography and biochemical analysis, the team created a model of proline dehydrogenase that can tell scientists more about the molecule's structure and functions. (sciencedaily.com)
  • Learnings from Comparative Analysis using SPR, NMR, & X-Ray Crystallography to Optimize Fragment Screening in Drug Discovery. (uni-marburg.de)
  • These results, together with a thorough biochemical and mutational study of RecN, allow us to propose a model for the role of RecN in DSB repair. (rcsb.org)
  • Using X ray crystallography the team could determine the 3D architecture of this key component at the atomic level. (le.ac.uk)
  • Since 2015 he has been the PI responsible for the Electron Crystallography research line of the Istituto Italiano di Tecnologia. (iucr.org)
  • The biochemical and physical data obtained here can be used for the structure based drug design. (nii.ac.jp)
  • NMR, cryoEM and X-ray crystallography), its success hinges on the development of sophisticated software that converts the raw detector data to structural information. (lu.se)
  • X-ray crystallography provides structural information that is crucial for fragment optimization, however there are several criteria that must be met for a successful fragment screening. (uni-marburg.de)
  • Using biochemical and imaging techniques using a light microscope the team found that when they modified key amino acids, the familiar X-shaped chromosomes could not form any longer. (le.ac.uk)