Biochemical and biophysical processes. Medical search. Frequent questions

KBI's robust protein characterization capabilities, including conformational and structural analyses via biophysical techniques and mass spectrometry, provide "fast insight" into a client's unique product, enabling a development strategy that accelerates timelines and reduces risk. (kbibiopharma.com)
You have strong technical knowledge base and proven experience with biophysical/biochemical characterization, bioanalytical method development or biomolecule product development. (biospace.com)
You have hands-on experience and trouble-shooting proficiency with analytical and characterization techniques for biomolecules, such as column chromatographic (HPLC/UPLC), capillary electrophoresis and/or biophysical techniques (such as DLS, CD, FTIR, DSC, AUC) for protein characterization. (biospace.com)
Our detailed biophysical characterization of the molecular chaperone TAPBPR provided us with a high-resolution view of peptide exchange on MHC-I proteins and led to the development a high-throughput methodology for generating barcoded MHC tetramer libraries encompassing hundreds of unique antigen specificities. (upenn.edu)
Structural and biochemical characterization of human adenylosuccinate lyase (ADSL) and the R303C ADSL deficiency-associated mutation. (medlineplus.gov)

The superior osteoinductive properties of BMP-2/7 are a consequence of its direct positive effect on progenitor cell homing at the implantation site, which consequently leads to upregulation of cartilage and bone related genes and biochemical markers. (lu.se)

Due to their significant water content, hydrogels possess biophysical characteristics very similar to natural tissue, and serve as highly effective matrices for 3D cell culture. (sociable.co)
Excessive proliferation of keratinocytes, abnormalities in the differentiation process, and continuous shedding of the thickened epidermis are characteristics of psoriasis (Figures 1 (a) and 1 (b)) [ 2 ]. (hindawi.com)
A program that focuses on the scientific study of the biochemical and biophysical characteristics of drugs at the molecular level and their interaction with, and effects on, biological macromolecules and cellular structures and processes. (mymajors.com)

Having integrated process development and analytical development teams ensures a broad perspective to achieve product quality goals for each program. (kbibiopharma.com)
Lead formulation and drug product development activities for multiple programs, in collaboration with analytical and drug product process development and other cross functional teams. (biospace.com)
What distinguishes the HitS production process from that of other companies, Meins explained, is its early application of biophysical and biochemical analytical methods. (bioprocessintl.com)

This second edition, thoroughly revised to bring it up to date, describes how one of the most fundamental metabolic processes evolved and transformed the surface chemistry of the Earth. (princeton.edu)
Lorsch came to NIGMS from the Johns Hopkins University School of Medicine, where he was a professor in the department of biophysics and biophysical chemistry. (nih.gov)
CMPS offers undergraduate and Master's level courses in biochemistry and biochemical techniques, as well as various courses in biophysical chemistry, NMR spectroscopy, and general physical chemistry. (lu.se)
CMPS is a highly integrated center comprising the divisions of Biochemistry and Structural Biology (Faculty of Science) and Biophysical Chemistry (Faculty of Engineering, LTH). (lu.se)

The book focuses on recent biochemical and biophysical advances and the molecular biological techniques that have made them possible. (princeton.edu)
It then moves through biochemical and molecular biological patterns in aquatic photoautotrophs, physiological and ecological principles, and global biogeochemical cycles. (princeton.edu)
Our main goal is to understand how the molecular properties of proteins and membranes modulate cellular processes. (unifr.ch)
The energetics of these remodelling processes are governed by the interplay between specialized proteins and membrane properties, but in most cases, we still lack a detailed molecular explanation of how these processes can be modulated in a cellular environment. (unifr.ch)
Protein metal-binding sites are responsible for catalysing important biological processes, such as photosynthesis, respiration, water oxidation, molecular oxygen reduction and nitrogen fixation. (uncommondescent.com)
We want to understand the molecular mechanisms of biological processes by exploring the structure, dynamics, interactions and function of proteins. (lu.se)
Further evaluation of the molecular mechanisms responsible for this increased osteoinductivity at an early stage in the regeneration process indicated that the CHA+BMP-2/7 enhanced progenitor cell homing at the implantation site, upregulated the key transcriptomic determinants of bone formation, and increased the production of bone extracellular matrix components. (lu.se)

Currently, the main focus of the lab is to understand how specific lipids and membrane properties influence intracellular trafficking processes and fat storage in eukaryotic cells. (unifr.ch)
The class-I and class-II proteins of the Major Histocompatibility Complex play an essential role in immune surveillance, by displaying epitopic peptides derived from the processing of aberrant proteins on the cell surface, where they can be recognized through interactions with specialized receptors on T cells and Natural Killer cells. (upenn.edu)
Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (bioprocessintl.com)
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. (nih.gov)
Extravasation of cancer cells from the blood vessel, which involves the trans-endothelial migration and tissue invasion, remains a less well understood process in cancer metastasis [1]. (selectbiosciences.com)
In particular, cancer cells were observed to exhibit invadopodia-like protrusions during the invasion process and the presence of endothelial cells forming the vessel lumen was found to sustain the cancer cell invasion into the matrix. (selectbiosciences.com)

To do so, we develop new computational approaches to investigate soft matter in silico , and we combine these investigations with biochemical and biophysical approaches. (unifr.ch)
To gain an atomic-detail description of these processes and to characterize novel therapeutic targets, we integrate cutting-edge structural biology methods from Nuclear Magnetic Resonance Spectroscopy, complementary biophysical and biochemical techniques, computational modeling and functional immunoassays. (upenn.edu)
This provides the groundwork for PNNL's signature capabilities in developing and applying computational methods to study the properties and processes of systems in complex environments. (pnnl.gov)

However, it is now well recognized that both β -arrestins can also act as direct modulators of numerous cellular processes via either GPCR-dependent or -independent mechanisms. (aspetjournals.org)
Understanding the mechanisms underlying the extravasation process is of fundamental importance in developing therapeutic targets for the prevention of metastasis [2],[3]. (selectbiosciences.com)
The article proposes how music builds upon brain mechanisms that are used in multiple perceptual, cognitive, and motor processes. (frontiersin.org)
Compared to non-living systems, living organisms have more complex biochemical processes, which can be comprehended by studying the most important metabolic pathways and their control mechanisms. (lu.se)

Recent structural, biophysical, and biochemical studies have provided novel insights into how β -arrestins bind to activated GPCRs and downstream effector proteins. (aspetjournals.org)
Here we review recent insights into the biophysical, biochemical, and epigenetic processes that underlie regenerative healing in amphibians, focusing particularly on tail and limb regeneration in Xenopus . (frontiersin.org)
Equipped with insights from design processes, it may be possible to design new metalloproteins with improved properties such as higher stability and greater efficiency, or to impart them with functions not found in nature, for use in an even wider range of biotechnological and pharmaceutical applications. (uncommondescent.com)
This is such an example: "Equipped with insights from design processes, it may be possible to design new metalloproteins with improved properties such as higher stability and greater efficiency, or to impart them with functions not found in nature. (uncommondescent.com)
These unifying mechanistic insights contribute to understanding how music may have emerged through evolution from brain processes that earlier evolved to carry out more basic psychological functions. (frontiersin.org)

In ten chapters that are self-contained but that build upon information presented earlier, the book starts with a reductionist, biophysical description of the photosynthetic reactions. (princeton.edu)

The BOREAS DSP-05 team generated an NPP image over the BOREAS region from a process-based ecosystem model, the Boreal Ecosystem Productivity Simulator (BEPS). (ornl.gov)
BEPS uses principles of the Forest BioGeochemical Cycles (Forest-BGC) model [Running and Coughlan, 1988] for quantifying the biophysical processes governing ecosystem productivity. (ornl.gov)
The framework is made up of a number of modules (or submodels), each containing formulations of a relatively well-defined, related subset of ecosystem processes with a distinct spatial and/or temporal signature (characteristic scale). (lu.se)

Following a short summary of recent structural studies, this review primarily focuses on β -arrestin-regulated physiologic functions, with particular focus on the central nervous system and the roles of β -arrestins in carcinogenesis and key metabolic processes including the maintenance of glucose and energy homeostasis. (aspetjournals.org)

To better understand the pathogenic mechanism, therefore, it is important to characterize the entire aggregation process from the initial oligomerization to the formation and growth of fibrils. (biorxiv.org)
Many technologies are used to characterize biological products, manufacturing processes, and raw materials. (bioprocessintl.com)

In this position, Lorsch oversees all of the Institute's mission-related activities, supporting basic research that increases understanding of biological processes and lays the foundation for advances in disease diagnosis, treatment, and prevention. (nih.gov)

and (3) adjustments of biophysical and biochemical parameters for the main boreal land cover types. (ornl.gov)
2) land cover information derived from satellite data is used to set biophysical and biochemical parameters [Cihlar et al. (ornl.gov)

Astrocytes are fundamental components of brain information processing and possess the ability to respond to synaptic signaling with increases in cytoplasmic calcium and modulate neuronal activity with the subsequent release of neuroactive transmitters. (nature.com)
The present review will examine evidence revealing that astrocytes respond to dopamine and modulate information processing in the primary brain regions implicated in the mesolimbic dopamine system. (nature.com)
By combining coarse grain MD simulations and biochemical and cellular experiments, we investigate how membrane properties modulate remodelling processes and how this might influence cell functioning. (unifr.ch)

Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. (nih.gov)

By leveraging the experience gained from developing processes for more than 75 unique biomolecules, KBI has perfected standard approaches for process development across various classes of proteins, including monoclonal antibodies. (kbibiopharma.com)

Using a combination of MD simulations and biochemical approaches, we study how membrane properties regulate protein functions, and hence how lipid metabolism may play a role in unexpected, and apparently unrelated, trafficking processes. (unifr.ch)
and adaptive learning approaches to process data, build models, and determine interventions," according to the program description. (sociable.co)

Zikanova M, Skopova V, Hnizda A, Krijt J, Kmoch S. Biochemical and structural analysis of 14 mutant adsl enzyme complexes and correlation to phenotypic heterogeneity of adenylosuccinate lyase deficiency. (medlineplus.gov)

Indeed, the water deficit imposes itself as extrinsic factor that negatively influences the germination of seeds due to the essential and vital role of water in the induction of recovery of metabolic activity during germination process. (scirp.org)

The HitS production process comprises steps for construct design, cloning and expression studies, purification evaluation, and quality control (QC) assessment. (bioprocessintl.com)
After FBDD, "hits" can advance to biophysical evaluation, in which methods such as nuclear magnetic resonance (NMR) and X-ray crystallography are used to confirm or dismiss earlier findings. (bioprocessintl.com)
As with FBDD, DEL hits advance to biophysical evaluation. (bioprocessintl.com)

Hydrogels represent a class of biomaterials that have great promise for these applications, particularly due to our ability to engineer their biophysical and biochemical properties and the potential for cell encapsulation during printing. (selectbiosciences.com)

Specific focus is given to outstanding problems in land surface boundary layer descriptions such as complex terrain, effects of horizontal heterogeneity on sub-meso-scale transport processes, energy balance closure, stable stratification and night time fluxes, dynamic interactions with atmosphere, plants (in canopy and above canopy) and soils. (copernicus.org)
Incorporation of hydroxyapatite (HA) in a collagen scaffold dramatically improves bone morphogenic protein (BMP) sequestration via biophysical interactions with BMP, thereby providing more controlled BMP release compared with pristine collagen. (lu.se)

Studies with β -arrestin mutant mice have identified numerous physiologic and pathophysiological processes regulated by β -arrestin-1 and/or -2. (aspetjournals.org)
Although fibrils are known to be toxic, a number of experimental studies also suggest that a subset of soluble oligomers, transiently appearing during the aggregation process, are more responsible for disease pathology than the fibril itself 2 - 6 . (biorxiv.org)
Metalloprotein design is not just an intellectual exercise that duplicates biochemical and biophysical studies of native metalloproteins. (uncommondescent.com)
Whereas biochemical and biophysical studies mostly reveal individual features that result in a loss of function, design requires the incorporation of all the structural features needed to attain a function. (uncommondescent.com)
The Reinberg lab studies the processes shaping chromatin structure and the transcriptional outcome establishing cellular identity. (chicagobiomedicalconsortium.org)

Fast" process, such as photosynthesis, respiration and stomatal regulation are implemented on a time step of one day (or one month, interpolating between output values for adjacent months to obtain a value for each day). (lu.se)

Beginning with harvest of material from a bioreactor, downstream processing removes or reduces contaminants to acceptable levels through several steps that typically include centrifugation, filtration, and/or chromatographic technologies. (bioprocessintl.com)

To dissect the mechanics of translation initiation, Lorsch and collaborators developed a yeast-based system and a wide variety of biochemical and biophysical methods. (nih.gov)
Around 1,200 have been produced at assay grade and verified for biophysical methods. (bioprocessintl.com)

Interestingly, according to the company, Vivostat® autologous fibrin sealant system "incorporates a unique and patented biochemical process that produces an autologous sealant from 120 ml of the patient's own blood in only 23 minutes. (medgadget.com)
This enzyme performs two steps in the process that produces (synthesizes) purine nucleotides. (medlineplus.gov)

Ventilatory strategies have been devised for different disease processes to protect pulmonary parenchyma while maintaining adequate gas exchange, and they may be responsible for the increased rates of survival for pathologies such as acute respiratory distress syndrome (ARDS). (medscape.com)

Astrocytes are among the most abundant cell types in the mammalian brain and garnered their name due to their star-like shape with a cell body and processes that can extend to contact thousands of synapses [ 1 ]. (nature.com)
In both processes, rapid proliferation gives rise to new tissue, cell fate has to be specified within that tissue, and distinct positional identities have to be established to generate a properly patterned structure. (frontiersin.org)
Combining this microfluidic platform with an automated image analysis algorithm, cancer cell morphological changes during the migration process were quantified and they were found to be microenvironment-dependent. (selectbiosciences.com)

In this initial study, we combine single-molecule Förster resonance energy transfer (FRET) spectroscopy, fluorescence lifetime imaging (FLIM), and image analysis using deep learning (codes are available at https://github.com/hoisunglab/FNet ) to interrogate several steps during the aggregation process of the 42-residue Aβ peptide (Aβ42), including dimerization, formation of stable oligomers, and fibril elongation. (biorxiv.org)
Today, the unit provides a rapid and cost-effective platform for protein generation, screening, biophysical and biochemical analysis, structural determination, and "hit" confirmation. (bioprocessintl.com)
Biochemical and biophysical analysis of five disease-associated human adenylosuccinate lyase mutants. (medlineplus.gov)

Enzyme controlled process which requires oxygen to produce 38 ATP from the breakdown of glucose. (lemonade-ed.com)

The aim of this research is to investigate breast cancer extravasation in vitro, using a microfluidic system with adjustable biophysical and biochemical factors, to mimic the 3D in vivo vascular microenvironment. (selectbiosciences.com)

A more fundamental reason for the difficulty may be the heterogeneity of the aggregation process. (biorxiv.org)

The Sgourakis Lab investigates the processes that enable adaptive immune responses against infectious diseases and cancer. (upenn.edu)

This approach has at least two weaknesses: 1) it relies on uncertain site-specific threshold values and 2) it does not consider changes in processes hindering the flow coupling to the surface. (copernicus.org)
My explanations for the above includes specific biochemical or biophysical processes. (lemonade-ed.com)

Conceptual representation of LPJ-GUESS showing the main process, time steps, state variables, and input environmental data. (lu.se)

At MAX IV's BioMAX beamline, an international team of scientists has determined important rate-limiting steps of lignocellulose breakdown, a major hurdle in efficient biomass processing. (lu.se)

We will ensure that individuals with disabilities are provided reasonable accommodations to participate in the job application process. (biospace.com)

Despite tremendous effort to understand aggregation of Aβ to form oligomers and fibrils, experimental results vary widely and there is no consensus on the model for these processes 7 . (biorxiv.org)

I can explain why life processes are affected if the negative feedback loop stops in a hot environment. (lemonade-ed.com)

We believe that the use of NASA LED for light therapy alone, and in conjunction with hyperbaric oxygen, will greatly enhance the natural wound healing process, and more quickly return the patient to a preinjury/illness level of activity. (researchgate.net)

The "slow" processes of individual allocation and growth, population dynamics and disturbance are implemented once each simulation year (Figure 1). (lu.se)

Characterization5

Genes1

Characteristics3

Analytical3

Chemistry4

Molecular7

Cells6

Computational3

Mechanisms4

Insights5

Photosynthetic1

Ecosystem3

Focuses1

Characterize2

Biological processes1

Parameters2

Modulate3

Quantitative1

Proteins1

Approaches2

Structural analysis1

Metabolic1

Evaluation3

Properties1

Interactions2

Studies5

Photosynthesis1

Downstream1

Methods2

Produces2

Disease processes1

Cell3

Analysis3

Enzyme1

System1

Fundamental1

Adaptive1

Specific2

Main1

Efficient1

Application1

Understand1

Environment1

Natural1

Individual1