Binds to importin. Medical search. Frequent questions

Dive into the research topics of 'Hantaan virus nucleocapsid protein binds to importin α proteins and inhibits tumor necrosis factor alpha-induced activation of nuclear factor kappa B'. Together they form a unique fingerprint. (mssm.edu)
The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. (wikipedia.org)
importin α is a typical nuclear import receptor which transport hundreds of functional proteins into the nucleus. (oist.jp)
Surprisingly, importin α interacts simultaneously with cargo proteins and DNA, suggesting that importin α plays a role not only in nuclear import but also in the functional regulation of cargo proteins on DNA. (oist.jp)
Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
containing proteins include the Ran-GTP binding importin and exportin cargo transport proteins. (cellsignal.com)
We previously reported that importin 13 (IPO13), a member of the importin-β family of nuclear import proteins, regulates nuclear import of the glucocorticoid receptor in airway epithelial cells, IPO13 serves as a potential marker for corneal epithelial progenitor cells, and IPO13 is associated with corneal cell proliferation. (molvis.org)
Nucleocytoplasmic transport is an essential phenomenon in eukaryotic cells controlled by the family of proteins called importins and exportins. (molvis.org)
Importin beta is a form of karyopherin that helps cargo proteins get into the nucleus. (prospecbio.com)
Without the aid of the importin alpha adapter protein, Importin beta can transport proteins into the nucleus. (prospecbio.com)
In humans there exist five isoforms of the B56 type regulatory subunit and they bind to their interacting proteins through a conserved LxxIxE motif. (eu.org)
Most of the B56 binding proteins contain a conserved motif LxxIxE which acts as a docking site for B56. (eu.org)
These superhelical structures present an extensive solvent-accessible surface that is well suited to binding large substrates such as proteins and nucleic acids. (eu.org)
Domains and repeats with an ARM-like fold have been found in a number of proteins, including: ARM repeat domain, found in beta-catenins, importins, karyopherin and exportins. (eu.org)
https://doi.org/10.1083/jcb.201604025 ) show that Importin-11 traffics the tumor suppressor PTEN into the nucleus and in so doing protects it from cytoplasmic proteins that cause PTEN degradation. (rupress.org)
It is now clear that Armadillo and beta-catenin bind directly to members of the T-cell factor/lymphoid enhancer factor subfamily of HMG box DNA-binding proteins, forming bipartite transcription factors that regulate Wingless/Wnt responsive genes in both Drosophila and vertebrates. (embl.de)
In this work, we used chromatin immunoprecipitation (ChIP) with an anti-nitro-tyrosine antibody followed by mass spectrometry to identify nitrated DNA binding proteins in T. cruzi and to detect alterations in nitration levels induced upon parasite incubation with the ECM. (omicsdi.org)
Histone H1, H2B, H2A and H3 were detected among the 9 most abundant nitrated DNA binding proteins using this proteomic approach. (omicsdi.org)
Finally, we used ChIP-Seq to map global changes in the DNA binding profile of nitrated proteins. (omicsdi.org)
We observed a significant change in the binding pattern of nitrated proteins to DNA after parasite incubation with ECM. (omicsdi.org)
This work provides the first global profile of nitrated DNA binding proteins in T. cruzi and additional evidence for modification in the nitration profile of histones upon parasite incubation with ECM. (omicsdi.org)
It has recently been found that arginine-containing dipeptide repeat proteins (R-DPRs), translated from C9orf72 repeat expansions, directly bind to several importins. (rug.nl)
Yet not all nuclear proteins interact with importins, necessitating the identification of a general importin-independent nuclear import pathway. (ox.ac.uk)
Here, we identify a code that determines importin-independent nuclear import of ankyrin repeats (ARs), a structural motif found in over 250 human proteins with diverse functions. (ox.ac.uk)
AR-containing proteins (ARPs) with a hydrophobic residue at the 13th position of two consecutive ARs bind RanGDP efficiently, and consequently enter the nucleus. (ox.ac.uk)
Importantly, circRNAs usually bind miRNAs to modulate the levels of downstream proteins, acting as competing endogenous RNA (ceRNA) and participating in tumor progression [ 11 ]. (biomedcentral.com)
Proteins bearing a classical nuclear localization signal (cNLS) are imported into the nucleus by the importin / heterodimer (26, 49, 55). (irjs.info)
Proteins bearing ncNLSs directly bind to one of the approximately 20 members of the importin family present in higher eukaryotes (72). (irjs.info)

An intramolecular interaction between the importin beta-binding (IBB) domain and the NLS-binding pocket of Importin subunit alpha (SRP1) prevents binding of NLS cargo (e.g. (eu.org)
Large T antigen ) in the absence of Importin subunit beta-1 (KAP95) by hiding of the NLS-binding pocket. (eu.org)
Binding of the IBB of Importin subunit alpha (SRP1) to Importin subunit beta-1 (KAP95) relieves this auto-inhibitory interaction and increases the affinity of Importin subunit alpha (SRP1) for NLS cargo. (eu.org)
Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. (nih.gov)

Rev is imported into the nucleus by the host protein importin β (Impβ), but how Rev associates with Impβ is poorly understood. (nih.gov)
Furthermore, we were able to detect an interaction between HTNV N protein and importin α, a nuclear import molecule responsible for shuttling NF-κB to the nucleus. (mssm.edu)
NP is imported into the nucleus by the importin-α/β pathway, through a direct interaction with importin-α isoforms. (nih.gov)
These results suggest an important mechanism by which importin-α and 14-3-3 control movement of ChREBP in and out of the nucleus in response to changes in glucose levels in liver and thus further suggest that the extended NLS of ChREBP is a critical glucose-sensing, glucose-responsive site. (elsevierpure.com)
First, it binds importin alpha, a form of karyopherin that binds the cargo protein in the cytoplasm, before the cargo protein is imported into the nucleus through the nuclear pore driven by energy from the Ran gradient. (prospecbio.com)
Importins load cargoes in the absence of PCI-32765 distributor Ran in the cytoplasm and release their cargo upon RanGTP binding in the nucleus (27, 33, 58). (irjs.info)
In contrast, exportins bind substrates only in the presence of RanGTP in the nucleus and cargo release is accomplished when the Ran-bound GTP molecule is hydrolyzed in the cytoplasm (10, 22, 39). (irjs.info)
At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. (nih.gov)
The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. (nih.gov)

The largest class of nuclear transport receptors is the superfamily of importin -like factors (also named karyopherins) that PCI-32765 distributor can be classified as importins (import karyopherin) and exportins (export karyopherin) depending on the direction in which they transport the cargo (reviewed PCI-32765 distributor in references 25, 32, 43, 72, and 80). (irjs.info)

To gain insight into how this can affect nucleocytoplasmic transport, we use coarse-grained molecular dynamics simulations to study the molecular interaction of poly-PR, the most toxic DPR, with several Kapβs (importins and exportins). (rug.nl)
We also show that poly-PR binds to exportins, especially at lower salt concentrations, interacting with several RanGTP and FG-Nup binding sites. (rug.nl)
Cargo binding and release of importins and exportins is controlled by a steep RanGTP gradient, which is maintained across the nuclear envelope through the asymmetric distribution of factors that regulate the guanine nucleotide-bound state of Ran (25, 41, PCI-32765 distributor 43, 47, 76). (irjs.info)

The nuclear import of steroids, bound to their receptor, is mediated by importins alpha and beta. (endocrine-abstracts.org)
DYRK1B inhibits the Sonic Hedgehog and WNT pathways, increasing the expression of master adipogenic transcription factors CCAAT/enhancer binding protein (C/EBP-alpha) and peroxisome proliferator-activated receptor gamma (PPAR-gamma) [ 2 ]. (biomedcentral.com)

A code for RanGDP binding in ankyrin repeats defines a nuclear import pathway. (ox.ac.uk)
The RaDAR (RanGDP/AR) pathway represents a general importin-independent nuclear import pathway and is frequently used by AR-containing transcriptional regulators, especially those regulating NF-κB/p53. (ox.ac.uk)

Importin 13 (IPO13), a member of the importin-β superfamily, was originally identified in the fetal rat lung and differentially expressed in cells of epithelial and mesenchymal origin. (molvis.org)

This work helps explain the nuclear accumulation of PTEN observed in many healthy tissues and, because Ipo11 mutant mice develop lung tumors, also implicates Importin-11 as a novel tumor suppressor. (rupress.org)
In these roles, it binds to cadherins, Tcf-family transcription factors, and the tumor suppressor gene product Adenomatous Polyposis Coli (APC). (embl.de)

The motif binds to a well conserved and surface-exposed binding groove formed between the B56 HEAT repeats 3 and 4. (eu.org)
In plakoglobin arm repeats bind alpha-catenin and N-cadherin. (embl.de)
Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. (nih.gov)

Here we provide a combined nuclear magnetic resonance and small-angle X-ray scattering (NMR/SAXS) analysis to describe the dynamics of the interaction between influenza B NP and the human importin-α. (nih.gov)
The observed binding mode is maintained in the context of heterotrimeric influenza polymerase, placing ANP32A in the immediate vicinity of known host-adaptive PB2 mutants. (nature.com)

The motif is found throughout eukaryotes and and in some viruses, which emphasises the general mechanism of B56 binding and its pivotal role in many cellular functions. (eu.org)

Importin α-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion. (southernbiotech.com)

Human ANP32A IDD transiently binds to the 627 domain, exploiting multivalency to maximise affinity. (nature.com)
A higher degree of flexibility is observed in the intervening and surrounding positions and a stronger binding affinity is observed when the residues at the position P2 or P7-P9 were either phosphorylated or an acidic residue is already present, as the negative charge engages with the basic patch of B56 ( Wang,2016 ). (eu.org)

The transcriptional activator NF-Y is a heterotrimeric complex made up of NF-YA, NF-YB, and NF-YC, which specifically binds the CCAAT consensus within about 30% of eukaryotic promoters. (irjs.info)

The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. (wikipedia.org)
Interestingly, the DNA binding domain of importin α is a chameleon sequence that changes its structure depending on the environments and it regulates each step of nuclear transport function of importin α by interacting with several molecules. (oist.jp)
We will also review the function and properties of the chameleon sequence of importin α and propose a new model of chromatin regulation focusing on the crosstalk between nuclear transport and DNA binding. (oist.jp)
Although unrelated in sequence, the beta-catenin binding regions of cadherins, Tcfs, and APC are acidic and are proposed to interact with this groove. (embl.de)

These findings shed light on the molecular basis of Rev recognition by Impβ and highlight an atypical binding behavior that distinguishes Rev from canonical cellular Impβ cargos. (nih.gov)
Therefore, a multifaceted perspective is necessary to understand the role of importin α in cellular activities. (oist.jp)

Inhibition of nuclear import of Large T antigen by phosphorylation-dependent (T124) binding of BRCA1-associated protein (BRAP) . (eu.org)

Replacing Lys 159 /Lys 190 residues of ChREBP with alanine resulted in loss of importin-α binding, glucose-stimulated transcriptional activity and nuclear localization.Asecondary 14-3-3 protein binding site also was identified, the α3 helix (residues 170-190) phospho-Ser 196 domain. (elsevierpure.com)
Polypyrimidine tract binding protein and associated splicing factor (PSF) has diverse functions inside the cell such as transcriptional regulation, DNA repair, acts as nucleic acids binding protein and regulate replication and infectivity of different viruses. (biomedcentral.com)

Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
mRNA is exported in the form of very large mRNP complexes, which begin to form during RNA processing when the transcription export (TREX) complex binds to the mRNA. (prospecbio.com)

This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. (wikipedia.org)

MIF4G domain-like, found in eukaryotic initiation factor eIF4G, translation initiation factor eIF-2b epsilon and nuclear cap-binding protein CBP80. (eu.org)

Predicted to enable importin-alpha family protein binding activity. (nih.gov)
Regulated Binding of Importin-alpha to PKC delta in Response to Apoptotic Signals Facilitates Nuclear Import. (cuanschutz.edu)

Poly-PR makes contact with the inner surface of most importins, which strongly interferes with Kapβ binding to cargo-NLS, IBB, and RanGTP in a poly-PR length-dependent manner. (rug.nl)

The protein binding study identified the association of host protein PSF with HIV-1 integrase. (biomedcentral.com)

Carbohydrate response element-binding protein (ChREBP) is a glucose-responsive transcription factor that plays a critical role in the glucose-mediated induction of genes involved in hepatic glycolysis and lipogenesis. (elsevierpure.com)
Human cells have seven family members of importin α genes that have cargo specificities, and these family members are differentially expressed depending on the tissue type. (oist.jp)
Encodes a MYB transcription factor that possesses an R2R3 MYB DNA binding domain and is known to regulate the expression of salt- and dehydration-responsive genes. (or.jp)

L..[IVLWC].E). One of, but not both, the amino acids in position P1 or P4 of the motif can be a variable hydrophobic residue for B56 binding, while P6 has the invariant glutamic acid. (eu.org)
We show that poly-PR-Kapβ binding depends on the net charge per residue (NCPR) of the Kapβ, salt concentration of the solvent, and poly-PR length. (rug.nl)

Although the C-terminal domain comprises a small percentage of the total protein, this domain in some cases (i) has PSD-95/Discs Large/ZO-1-binding motifs that may be important for protein-protein interactions (e.g. (tcdb.org)
PHAT domain, found in the RNA-binding protein Smaug. (eu.org)
protein_coding" "Cz01g27080.t1","No alias","Chromochloris zofingiensis","Solute-binding protein family 5 domain [Interproscan]. (ntu.edu.sg)

this heterodimer then forms a complex with importin-β to interact with the nuclear pore complex. (elsevierpure.com)

Cross-linking mass spectrometry and compensatory mutagenesis data combined with molecular docking simulations suggest a structural model in which one Rev monomer binds to the C-terminal half of Impβ with Rev helix α2 roughly parallel to the HEAT-repeat superhelical axis, whereas the other monomer binds to the N-terminal half. (nih.gov)

The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)

Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP. (harvard.edu)

Importin-α and 14-3-3 were found to bind competitively to this secondary site. (elsevierpure.com)
And circPAPD4 promoted CREBZF expression by competitively binding to miR-1269a. (biomedcentral.com)

In this work, we recharacterized the importin-α binding nuclear localization signal (NLS) of rat ChREBP, identifying it as an extended classical bipartite NLS encompassing minimally residues 158-190. (elsevierpure.com)

We recently discovered that importin α directly binds DNA and revealed the unique binding property. (oist.jp)

Importin α binds multiple DNA sequences with different affinities, and efficiently slides along DNA. (oist.jp)

Nuclear localization of MYB4 depends on the action of the beta importin SAD2. (or.jp)

Longer poly-PRs at higher concentrations are also able to make contact with the outer surface of importins that contain several binding sites to FG-Nups. (rug.nl)
Serum insulin-like growth factor (IGF)-I and IGF-binding protein-3 concentrations and prostate cancer risk: results from the European Prospective Investigation into Cancer and Nutrition. (mdc-berlin.de)

We show that Impβ binds two Rev monomers through independent binding sites, in contrast to the 1:1 binding stoichiometry observed for most Impβ cargos. (nih.gov)

We will review the nuclear transport function of importin α and present our recent findings on the function of DNA binding. (oist.jp)

Meanwhile, various non-transport functions of importin α have been reported. (oist.jp)

Anatomy25

Organisms22

Diseases1

Chemicals and Drugs106

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes75

Technology, Industry, Agriculture1

Information Science4

Health Care1

Proteins28

SUBUNIT4

Nucleus9

Karyopherin1

Exportins3

Receptor2

Pathway2

Superfamily1

Tumor2

Repeats3

Influenza2

Mechanism1

Cytoplasmic1

Affinity2

CCAAT1

Sequence4

Cellular2

Phosphorylation-dependent1

Transcriptional2

MRNA2

Inhibits1

Eukaryotic1

Alpha2

RanGTP1

Host protein1

Genes3

Residue2

Domain3

Interact1

Mass spectrometry1

Nuclear cap binding1

Complex1

Competitively2

Residues1

Directly1

Efficiently1

Beta1

Concentrations2

Contrast1

Function1

Transport1