• When the cell is not dividing, separase is prevented from cleaving cohesin through its association with either securin or upon phosphorylation of a specific serine residue in separase by the cyclin-CDK complex. (wikipedia.org)
  • Separase phosphorylation leads to a stable association with CDK1-cyclin B1. (wikipedia.org)
  • The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. (bdbiosciences.com)
  • 5) Formation of the Cdk1-cyclin B1-separase complex is counter-acted by phosphorylation of cyclin B1 in early mitosis. (uni-bayreuth.de)
  • Additionally, Cuc B brought on phosphorylation of your Chk1 downstream effector Cdc25C on Ser-216 and Cdk1 on Tyr15 had been also inhibited (Fig. 5C).Cuc B induced ROS generation and didn't influence STAT3 phosphorylationRecent research have shown that Cuc B induced intracellular ROS formation in HeLa, SW480, and B16F10 cells [14,15,39]. (achrinhibitor.com)
  • During G1, cyclin D-dependent kinase-mediated phosphorylation of Rb at Ser-795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues. (affbiotech.com)
  • Studies with Mcl-1 phosphorylation mutants show that cyclin E/Cdk2-dependent phosphorylation of Mcl-1 residues on its PEST domain resulted in increased Mcl-1 stability (Thr92, and Thr163) and Bim binding (Ser64). (oncotarget.com)
  • On the entry of mitosis phosphorylation by cdk1/cyclin B network marketing leads to inactivation of UBF1 and SL1. (cancerrealitycheck.com)
  • UBF1 is normally turned on during G1 development by phosphorylation of serine 484 by cdk 4/cyclin D1 (42) and serine 388 by cdk2/cyclin E and A (41) leading to complete activation of rRNA transcription. (cancerrealitycheck.com)
  • Furthermore, roscovitine stabilized an unusual collapsed conformation of the glycine-rich loop, an important site of CDK regulation, and we report an investigation of the effects of glycine-rich loop phosphorylation on roscovitine binding. (rcsb.org)
  • the cyclin B-cdk 1 complex) is activated at M-phase onset by removal of inhibitory phosphorylation of cdk1 at thr-14 and tyr-15. (ox.ac.uk)
  • Using the in vitro cell cycle system derived form Xenopus eggs, here we show, however, that inhibitory phosphorylation of cdk1 contributes to control MPF activity during M-phase exit. (ox.ac.uk)
  • By sampling extracts at very short intervals during both meiotic and mitotic exit, we found that cyclin B1-associated cdk1 underwent transient inhibitory phosphorylation at tyr-15 and that cyclin B1-cdk1 activity fell more rapidly than the cyclin B1 content. (ox.ac.uk)
  • Inhibitory phosphorylation of MPF correlated with phosphorylation changes of cdc25C, the MPF phosphatase, and physical interaction of cdk1 with wee1, the MPF kinase, during M-phase exit. (ox.ac.uk)
  • Treatment of M-phase extracts with a mutant cyclin B1-cdk1AF complex, refractory to inhibition by phosphorylation, impaired binding of the Anaphase Promoting Complex/Cyclosome (APC/C) to its co-activator Cdc20 and altered M-phase exit. (ox.ac.uk)
  • Here we compare the specificity of two budding yeast cyclins, the S-phase cyclin Clb5 and the M-phase cyclin Clb2, in the phosphorylation of 150 Cdk1 (Cdc28) substrates. (nature.com)
  • Although we did not identify any highly Clb2-specific substrates, we found that Clb2-Cdk1 possessed higher intrinsic kinase activity than Clb5-Cdk1, enabling efficient phosphorylation of a broad range of mitotic Cdk1 targets. (nature.com)
  • We initially found that this function of EZH2 can be activated through a protein modification called phosphorylation, which was catalyzed by enzymes termed cyclin-dependent kinase 1 and 2 (CDK1/2) (Nat Cell Biol 12: 1108-1114, 2010). (elsevierpure.com)
  • Cdk2 promotes DNA replication and is a promising cancer therapeutic target, but its functions appear redundant with Cdk1, an essential Cdk affected by most Cdk2 inhibitors. (rcsb.org)
  • Bypassing inhibition with ir-Cdk2 or with Cdk1 shows that Cdk2 is rate-limiting for replication in this system because Cdk1 is insufficiently active. (rcsb.org)
  • Additionally, crystal structures and kinetics reveal alternative binding modes of Cdk1-selective and Cdk2-selective inhibitors and mechanisms of Cdk2 inhibitor resistance. (rcsb.org)
  • Cyclin A is involved in both S-phase and G2/M transitions of the cell cycle through its association with cdk2 and cdk1, respectively. (bdbiosciences.com)
  • Fig. 2: p38γ compensates for the loss of CDK1 or CDK2. (nature.com)
  • CDK2 is regulated by cyclin E or cyclin A. Upon activation by cyclin E, it phosphorylates the retinoblastoma (pRb) protein which activates E2F mediated transcription and allows cells to move into S phase. (umbc.edu)
  • The CDK2/cyclin A complex plays a role in regulating DNA replication. (umbc.edu)
  • Cyclin E/Cdk2 kinase activity is frequently deregulated in human cancers, resulting in impaired apoptosis. (oncotarget.com)
  • Here, we show that cyclin E/Cdk2 phosphorylates and stabilizes the pro-survival Bcl-2 family protein Mcl-1, a key cell death resistance determinant to the small molecule Bcl-2 family inhibitors ABT-199 and ABT-737, mimetics of the Bcl-2 homology domain 3 (BH3). (oncotarget.com)
  • Cyclin E levels were elevated and there was increased association of cyclin E/Cdk2 with Mcl-1 in ABT-737-resistant compared to parental cells. (oncotarget.com)
  • In HCMV-infected cells, particular subsets of CDK-cyclin complexes are downregulated/suppressed (CDK4-cyclin D, CDK6-cyclin D, CDK2-cyclin A) or upregulated/turned on (CDK1-cyclin B, CDK2-cyclin E), leading to an early on S stage arrest termed pseudomitosis [11] eventually. (niepokorny.org)
  • Predicated on series evaluation and a 3D style of pUL97, the viral kinase displays structural similarity to CDK2 in the catalytic middle and in functionally essential residues from the ATP binding site [16]. (niepokorny.org)
  • Identification of a cyclin-cdk2 recognition motif present in substrates and p21-like cyclin-dependent kinase inhibitors. (nature.com)
  • The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. (affbiotech.cn)
  • Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. (affbiotech.cn)
  • Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. (affbiotech.cn)
  • Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. (bdbiosciences.com)
  • CDKs show their action by interacting with cyclins and different CDK-cyclin complexes regulate the cell cycle in the G1, S and G2/M phases ( 1 ). (spandidos-publications.com)
  • CDKs belong to a large family of STKs that are regulated by their cognate cyclins. (umbc.edu)
  • In addition with their main function in the legislation of cell routine progression, particular types of CDKs and cyclins get excited about transcription also, splicing, epigenetic legislation, neuronal features, stem cell regeneration, spermatogenesis, and differentiation [10]. (niepokorny.org)
  • Along with Cdk1/cyclin N, members of the Aurora/Ipl1 kinase family are also crucial regulators of mitosis. (smadpathway.com)
  • Separase can alternatively be inhibited by association with Cdk1-cyclin B1 but the corresponding complex is scarce in early mitosis and cannot explain why vertebrate securin is dispensable. (uni-bayreuth.de)
  • While free securin is phosphorylated in mitosis, separase-bound securin is kept in an unphosphorylated state by associated PP2A. (uni-bayreuth.de)
  • Dephosphorylation results in a second peak of Cdk1-cyclin B1-separase complex formation in late mitosis when most cyclin B1 has already been degraded. (uni-bayreuth.de)
  • GBF1 is phosphorylated by CDK1-cyclin B in mitosis, which results in its dissociation from Golgi membranes. (aston.ac.uk)
  • Complete ablation of Cdk1 precludes the entry of G2 phase cells into mitosis, and is early embryonic lethal in mice. (eur.nl)
  • These problems start early in mitosis and are alleviated by depletion of isoform 1 of PRC1 (PRC1-1), by gene ablation of its binding partner KIF4, or by abrogation of KIF4 motor activity. (eur.nl)
  • The activator Cdc20 is then replaced by a second activator, Cdh1, and APC/C Cdh1 promotes complete degradation of M cyclin, followed by polo-like kinase 1, Aurora A, and other substrates, to complete mitosis and cytokinesis and drive progression into G1 [ 1 , 2 ]. (biomedcentral.com)
  • Fisher, D. L. & Nurse, P. A single fission yeast mitotic cyclin B p34 cdc2 kinase promotes both S-phase and mitosis in the absence of G1 cyclins. (nature.com)
  • In both complexes, separase is inhibited by pseudosubstrate motifs that block substrate binding at the catalytic site and at nearby docking sites. (wikipedia.org)
  • Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb), nucleolin and others. (bdbiosciences.com)
  • Lundberg, A. S. & Weinberg, R. A. Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes. (nature.com)
  • The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling. (lookformedical.com)
  • New anticancer therapy strategies refer to the inhibition of CDK-cyclin complexes as an important target to prevent uncontrolled proliferation and induce apoptosis in cancer cells ( 2 ). (spandidos-publications.com)
  • Here we show that this interaction occurs only in proliferating cells where the two proteins co-associate with 5'-cap-binding protein complexes, eIF4F or the plant specific eIFiso4F. (aber.ac.uk)
  • A- and B-type cyclins differentially modulate substrate specificity of cyclin-cdk complexes. (nature.com)
  • Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. (affbiotech.cn)
  • Dampening Cdk1 activation, by reducing gene expression or upon treatment with cell-permeable Cdk1 inhibitors, is also detrimental for proliferating cells, but has been associated with defects in mitotic progression, and the formation of aneuploid daughter cells. (eur.nl)
  • We conclude that cell death in response to Cdk1 inhibitors directly relates to chromosome alignment defects generated by insufficient repression of PRC1-1 and KIF4 during prometaphase. (eur.nl)
  • Collectively, our findings identify a novel mechanism of cyclin E-mediated Mcl-1 regulation that provides a rationale for clinical use of Bcl-2 family and Cdk inhibitors for Mcl-1-dependent tumors. (oncotarget.com)
  • Current scientific trials are looking into cyclin-dependent kinase (CDK) inhibitors, such as for example roscovitine, an inhibitor of CDK1, -2, -5, -7, and -9, that lowers viral DNA synthesis, creation of late protein and infectious pathogen particles [4]. (niepokorny.org)
  • Recent studies have shown that cyclin-dependent kinase (CDK) inhibitors can have a tremendous impact on cell cycle progression in plants. (uni-bielefeld.de)
  • The cell cycle is a tightly regulated process that is controlled by the conserved cyclin-dependent kinase (CDK)-cyclin protein complex 1 . (nature.com)
  • Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. (spandidos-publications.com)
  • This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. (affbiotech.cn)
  • However, while securin contains its own pseudosubstrate motifs to occlude substrate binding, the CDK1-cyclin B complex inhibits separase by rigidifying pseudosubstrate motifs from flexible loops in separase itself, leading to an auto-inhibition of the proteolytic activity of separase. (wikipedia.org)
  • 4) Pin1 catalyzed trans-to-cis isomerization of separase is essential for Cdk1-cyclin B1-dependent inhibition and explains why the kinase and securin bind separase in a mutually exclusive manner. (uni-bayreuth.de)
  • Here, we used a large-scale RNAi screen to identify the human genes that critically determine the cellular toxicity of Cdk1 inhibition. (eur.nl)
  • We show that Cdk1 inhibition leads to fatal sister chromatid alignment errors and mitotic arrest in the spindle checkpoint. (eur.nl)
  • The proteins that bind the two sister chromatids, disallowing any premature sister chromatid separation, are a part of the cohesin protein family. (wikipedia.org)
  • Proteins which bind to DNA. (lookformedical.com)
  • The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
  • In Saccharomyces cerevisiae cells that lack NAP-1 the Clb2 LY2606368 (B-type cyclin) was LY2606368 unable to efficiently induce mitotic events [19 20 Over-expression of SET or CDA1 results in an inhibitory effect on cell cycle progression at the G2/M phase [18] suggesting that SET/NAP-containing proteins are cell cycle regulators. (careersfromscience.org)
  • UBF1 a series particular DNA binding proteins interacts using the template rDNA. (cancerrealitycheck.com)
  • SL1 a proteins Sotrastaurin (AEB071) complex filled with the TATA-binding proteins (TBP) and three Pol I-specific TBP-associated elements (TAFs) (9) confers promoter specificity. (cancerrealitycheck.com)
  • The participation of most three proteins have already been described as getting in charge of cell routine through alteration of or connections with key mobile regulator proteins such as for example p53 p21 cyclins aswell as transcription elements proto-oncogenes and development elements/ cytokines (20). (cancerrealitycheck.com)
  • About 24% of these proteins were phosphorylated more efficiently by Clb5-Cdk1 than Clb2-Cdk1. (nature.com)
  • While the two crystal structures suggested an elevator-like movement to afford alternating access to the substrate binding site, the mechanistic role of Na+ and substrate in the conformational isomerization remains unclear. (bvsalud.org)
  • Specific substrates generally bind with sufficient affinity that they remain bound during multiple ubiquitin transfer reactions, resulting in processive substrate modification [ 9 , 10 ]. (biomedcentral.com)
  • Schulman, B. A., Lindstrom, D. L. & Harlow, E. Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A. (nature.com)
  • Figure 1: Identification of Clb5-specific Cdk1 substrates in budding yeast. (nature.com)
  • To do so, they treated melanoma cells with a panel of small molecules and identified JNJ-7706621 (JNJ) as the best inhibitor of cyclinB1/cdk1. (medindia.net)
  • Donaldson, A. D. The yeast mitotic cyclin Clb2 cannot substitute for S phase cyclins in replication origin firing. (nature.com)
  • Previously, we found that eukaryotic initiation factor (eIF) 4A interacts with cyclin dependent kinase A (CDKA), the plant ortholog of mammalian CDK1. (aber.ac.uk)
  • Orthologous to several human genes including CCNB1 (cyclin B1). (nih.gov)
  • Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. (abcam.cn)
  • Along with CDK1 and -2, CDK7 and -9 are necessary for effective HCMV replication and had been discovered upregulated in HCMV-infected cells [3,12,13,14,15]. (niepokorny.org)
  • In 2021, structures of human separase were determined in complex with either securin or CDK1-cyclin B1-CKS1 using cryo-EM by scientists of the University of Geneva. (wikipedia.org)
  • Securin or CDK1-cyclin B binding is mutually exclusive. (wikipedia.org)
  • Silencing of the spindle assembly checkpoint (SAC) in metaphase enables the ubiquitin ligase APC/C to mediate the proteasomal destruction of securin (and cyclin B1), thereby activating separase. (uni-bayreuth.de)
  • It is enabled by SAC-activated Mad2 to bind and inhibit separase and can functionally replace securin. (uni-bayreuth.de)
  • Previous data suggested that Cuc B triggered cell cycle arrest by blocking the STAT3 signaling pathway, which resulted in reduced expression of downstream targets, like Cyclin B1, Cyclin A [402]. (achrinhibitor.com)
  • Targets of the cyclin-dependent kinase Cdk1. (nature.com)
  • MicroRNAs regulate gene expression at the post-transcriptional level by binding to 3′- or less often to 5′-untranslated regions of target messenger RNAs (mRNAs), which in consequence leads to inhibited translation and/or induces degradation of targeted mRNA [ 1 ]. (archivesofmedicalscience.com)
  • Enables cyclin-dependent protein serine/threonine kinase regulator activity. (nih.gov)
  • Our approach thus provides insight into structure, functions, and biochemistry of a cyclin-dependent kinase. (rcsb.org)
  • Cyclin-dependent kinase 1 (Cdk1) is absolutely essential for cell division. (eur.nl)
  • SET regulates the G2/M transition by modulating cyclin B-cyclin-dependent kinase 1 (CDK1) activity [18]. (careersfromscience.org)
  • Within this study, we showed that Cuc B induced G2/M arrest inside a ROS dependent manner without having affecting STAT3 in A549 cells: Cuc B induced ROSmediated DNA harm, which activated G2/M phase checkpoint through ATM-activated Chk1-Cdc25C-Cdk1 and -p53-14-3-3-s cascades. (achrinhibitor.com)
  • The model starts at mitotic entry initiated by the activities of Cyclin-dependent kinase 1 (CDK1) and Polo-like kinase 1 (PLK1), transitions through Anaphase-promoting complex (APC/C) bound to Cell division cycle protein 20 (CDC20), and ends upon mitotic exit mediated by APC/C bound to CDC20 homolog 1 (CDH1). (pharmafocusasia.com)
  • Cyclin E knock-down restored ABT-737 sensitivity to acquired and inherently resistant Mcl-1-dependent tumor cells. (oncotarget.com)
  • The NS5A-dependent IL25 antibody activation of rRNA transcription is apparently because of hyperphosphorylation and consequent activation of upstream binding aspect (UBF) a Pol I DNA binding transcription aspect. (cancerrealitycheck.com)
  • At M-phase exit, MPF is destroyed by ubiquitin-dependent cyclin proteolysis. (ox.ac.uk)
  • CDK3 is regulated by cyclin C and it phosphorylates pRB specifically during the G0/G1 transition. (umbc.edu)
  • Cyclin A may also form a complex with the adenovirus oncoprotein E1A which has DNA binding activity. (bdbiosciences.com)
  • Among various zinc-binding groups, hydroxamic acid shows the highest inhibitory activity of Class I HDACs, which was also reconfirmed by three-dimensional quantitative structure-activity relationship (3D-QSAR) pharmacophore prediction. (bvsalud.org)
  • Consistent with a reduced level of GBF1 activity at the Golgi membrane there is a reduction in levels of membrane-associated GTP-bound ARF in mitotic cells. (aston.ac.uk)
  • Our results show that, normally, Cdk1 activity must rise above the level required for mitotic entry. (eur.nl)
  • Cyclin A degradation employs preferentially used lysines and a cyclin box function other than Cdk1 binding. (nih.gov)
  • Purvalanol is a purine-derived CDKI that binds with a high selectivity and competitively to the ATP binding side of CDK1/2 and leads to G2/M cell cycle arrest ( 4 ). (spandidos-publications.com)
  • In the current study, Lu and her colleagues show that a protein complex named cyclin B1/cdk1, which is expressed at high levels in the cytoplasm of advanced melanomas, induces a pair of precise chemical modifications on iASPP to activate the protein. (medindia.net)
  • Separase, also known as separin, is a cysteine protease responsible for triggering anaphase by hydrolysing cohesin, which is the protein responsible for binding sister chromatids during the early stage of anaphase. (wikipedia.org)
  • Protein phosphatase 2A (PP2A) also binds separase but the physiological role of this interaction remains enigmatic. (uni-bayreuth.de)
  • 6) Upon destruction of this last cyclin B1 in early G1 phase, separase is released from the late Cdk1-cyclin B1-separase complex and triggers centriole disengagement, thereby licensing later centrosome duplication. (uni-bayreuth.de)
  • Tissue specificity of cyclin B expression in D. melanogaster]. (nih.gov)
  • Miller, M. E. & Cross, F. R. Cyclin specificity: how many wheels do you need on a unicycle? (nature.com)
  • Regulation through these distinct binding partners provides two layers of negative regulation to prevent inappropriate cohesin cleavage. (wikipedia.org)
  • Activation of STAT3 signaling could up-regulate Cyclin B1, c-Myc, Bcl-x and regulating cell growth and survival.Chk1 knockdown reversed Cuc B induced G2/M phase arrestTo dissect the downstream effector in Cuc B mediated G2/M phase arrest, the part of Chk1 was examined with Chk1 siRNA. (achrinhibitor.com)
  • 1993). p53 binds to two sites 2.4 kb and 1.4 kb upstream of the p21 promoter. (darkskiesfilm.com)
  • Filamin A (FLNa) can effect orthogonal branching of F-actin and bind many cellular constituents. (rupress.org)
  • Thermal stability assays show that ERKi do not destabilise ERK2 (or ERK1) in vitro, suggesting that ERK2 turnover is a cellular consequence of ERKi binding. (babraham.ac.uk)
  • Differentially CTCF-binding sites in cattle rumen tissue during weaning. (usda.gov)
  • e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens. (lookformedical.com)
  • The crystal structures of two bacterial homologues ASBTNM and ASBTYf have previously been determined, with the former showing an inward-facing conformation, and the latter adopting an outward-facing conformation accomplished by the substitution of the critical Na+-binding residue glutamate-254 with an alanine residue. (bvsalud.org)
  • The ATM activated Chk1-Cdc25C-Cdk1 pathway was additional investigated. (achrinhibitor.com)
  • Activation of p53 in response to DNA damage is associated with a rapid increase in its levels and with an increased ability of p53 to bind DNA and mediate transcriptional activation. (darkskiesfilm.com)
  • The two sister chromatids are initially bound together by the cohesin complex until the beginning of anaphase, at which point the mitotic spindle pulls the two sister chromatids apart, leaving each of the two daughter cells with an equivalent number of sister chromatids. (wikipedia.org)
  • Butyrate induces modifications of the CTCF-binding landscape in cattle cells. (usda.gov)
  • Despite the reduced levels of membrane bound GBF1 and ARF, COPI binding to the Golgi membrane appears unaffected in mitotic cells. (aston.ac.uk)
  • Fluorescence from leuko cytes bound to mesangial cells was determined by spectrophotometry. (sodiumchannel-signal.com)
  • A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
  • Transcription initiation by individual RNA Pol I needs at least two elements furthermore to Pol I: the upstream binding aspect 1 (UBF1) and a species-specific aspect SL1. (cancerrealitycheck.com)
  • UBF may activate rDNA transcription by recruiting Pol I towards the rDNA promoter stabilizing binding of SL1 and contending with nonspecific DNA binding protein such as for example histone H1 (21). (cancerrealitycheck.com)
  • Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
  • A retinoblastoma-binding protein that has an affinity for core HISTONES. (lookformedical.com)
  • METHODS: We report on a protein subunit vaccine comprising the receptor-binding domain (RBD) of the ancestral SARS-CoV-2 spike protein, dimerised with an immunoglobulin IgG1 Fc domain. (bvsalud.org)
  • FLNa dimeric subunits have N-terminal spectrin family F-actin binding domains (ABDs) and an elongated flexible segment of 24 immunoglobulin (Ig) repeats. (rupress.org)
  • Faha B, Ewen ME, Tsai LH, Livingston DM, Harlow E. Interaction between human cyclin A and adenovirus E1A-associated p107 protein. (bdbiosciences.com)
  • SET was initially identified LY2606368 in a patient with acute undifferentiated leukemia who harbored an intrachromosomal translocation on chromosome 9 [14-16] and demonstrated to bind B-type cyclins [17]. (careersfromscience.org)
  • ATCC CCL-243) were probed with the mouse anti-human cyclin A antibody at concentration of 2.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3). (bdbiosciences.com)
  • Human cyclin A has been reported to migrate between 54-60 kDa by SDS-PAGE and clone BF683 reportedly does not cross-react with mouse, rat or mink cyclin A. (bdbiosciences.com)
  • Cyclin E depletion in various human tumor cell-lines and cyclin E -/- mouse embryo fibroblasts showed decreased levels of Mcl-1 protein, with no change in Mcl-1 mRNA levels. (oncotarget.com)
  • We found that EZH2 binds to MALAT1, a cancer metastasis-related long non-coding RNA that has been implicated in many types of human cancer. (elsevierpure.com)
  • In the absence of cyclin E, Mcl-1 ubiquitination was enhanced, leading to decreased protein stability. (oncotarget.com)
  • A microRNA strand is transferred to the Argonaute complex (AGO), forming an RNA-induced silencing complex (RISC), and guides it to pair with the target mRNA through binding of the microRNA seed sequence with the microRNA recognition site on the mRNA. (archivesofmedicalscience.com)