Enriched BALB/c mouse splenic T cells were stimulated with PMA plus ionomycin for 6 hours and then stained with mouse CD154 (CD40L) (clone SA047C3) FITC (flled histogram) or rat IgG2a FITC isotype control (open histogram). (biolegend.com)
The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis. (biolegend.com)
Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation, partial inhibition of CD40 binding to CD40L, and prevention of B cell apoptosis. (biolegend.com)
CD40, as a single receptor that binds CD154 (CD40-ligand or CD40L), regulates counteractive effector functions such as production of pro- and anti-inflammatory cytokines. (sciencecentral.in)
Binding of CD40 and its natural ligand CD40L (CD154) triggers cytokine secretion, and increased expression of costimulatory molecules is required for T-cell activation and proliferation. (elsevierpure.com)
The antibody binds specifically to CD40L, a surface receptor expressed on activated T cells which acts as a costimulatory molecule to trigger immune responses. (absoluteantibody.com)
Also describes the generation of humanized monoclonal antibodies against CD40L and the use of a crystal structure to determine its binding. (absoluteantibody.com)
The CD40 ligand (CD40L) CD154 is a glycoprotein that is transiently expressed at high levels on the surface of CD4+ T cells when they are activated (2, 30, 39, 51, 53). (bioskinrevive.com)
CD40L is a member of the tumor necrosis factor family (2) and binds to CD40, a member of the tumor necrosis factor receptor family (60). (bioskinrevive.com)
Human test samples and standards of known CD40L concentration are added to these wells and allowed to complex with the bound CD40L antibody. (biosensis.com)
X-linked immunodeficiency with hyper-immunoglobulin M (XHIGM or HIGM1) is a rare form of primary immunodeficiency disease caused by mutations in the gene that codes for CD40 ligand (CD40L, also known as CD154 or TNFSF5 or gp39). (medscape.com)
The secondary antibody repertoire generation is antigen and T-cell dependent and occurs in peripheral lymphoid organs, mainly through the interaction between CD40L (CD154), expressed on activated CD4 + T cells, and CD40, expressed on B cells (see image below). (medscape.com)
The molecular target of TNX-1500 is CD40-ligand (CD40L), which is also known as CD154. (tonixpharma.com)
TNX-1500 is a third generation anti-CD40L mAb that has been designed by protein engineering to decrease FcγRII binding and to reduce the potential for thrombosis. (tonixpharma.com)
Its ligand, CD154 or CD40L, has a more restricted expression pattern and is found primarily on activated T cells, B cells, and platelets. (biomedcentral.com)
CD40 ligand (CD40L or CD154), a type II membrane glycoprotein and a member of the TNF superfamily, is known for its transient expression on activated CD4+ T lymphocytes which is responsible for the helper T cells' function on resting B cells in a non-antigen-dependent as well as non-major histocompatibility complex-restricted fashions. (abbiosciences.com)
CD40L is produced as homotrimeric membrane anchored protein each of the three membrane proximal stalk regions can be sequentially proteolyzed to generate the soluble trimeric protein which retains full binding activity to its receptor, CD40. (abbiosciences.com)
SIRPα-Fc-CD40L bound CD47 and CD40 with high affinity and activated CD40 signaling in the absence of Fc receptor cross-linking. (aacrjournals.org)
Ligand9
CD154 has been kown a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. (biolegend.com)
Son principal ligand, le CD154, appartient à la famille des TNFs (Tumor necrosis factor). (umontreal.ca)
Its main ligand, the CD154, belongs to the Tumor necrosis factors (TNFs) superfamily. (umontreal.ca)
In addition, several studies have demonstrated the beneficial effect of blocking the binding of CD40 to its major ligand CD154, in various diseases. (umontreal.ca)
CD154 (CD40 ligand, gp39) binds to CD40 expressed on antigen presenting cells and mediates important costimulatory functions between ≥ ≥ ≥ these cells. (cytekbio.com)
CD95 (Fas, APO-1, TNFRSF6) is a member of the TNF-receptor superfamily and is best known for its role in mediating activation-induced cell death in activated T cells following binding to its ligand, CD95L/FasL induced on antigen-presenting cells (APCs). (sanguinebio.com)
CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (3). (ancell.com)
The costimulatory molecule, CD40, upon interaction with its cognate ligand CD154 on T cells, promotes the expression of numerous cytokines/chemokines by the CD40 expressing cells. (grantome.com)
Receptor4
For most serotypes, adenovirus infection is mediated by the high-affinity binding of the fiber-knob region to a receptor of target cell, named as the coxsackie-Ad receptor (CAR), which mainly determines the viral tropism [44]. (genemedi.com)
In addition to innate immune responses in circulation, AdV virions can also be trapped by splenic macrophages (MFs) of the MARCO subset via the binding of fiber knob of the capsid to integrin b3 receptor [60]. (genemedi.com)
This binding process leads to the release of IL-1α and activation of IL-1 receptor, promoting chemokines production and attracting other innate immune cells to kill AdV infected MFs [61]. (genemedi.com)
The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. (cancer-genetics.org)
Antigen6
The invention relates to methods of modulating the antigen-binding affinity of an antibody by determining, using data corresponding to the structure of a complex between the antibody and an antigen in a solvent, a representation of a charge distribution of the CDRs of the antibody which minimizes electrostatic contribution to binding free energy between the antibody and the antigen in a solvent. (justia.com)
Guided by these determinations, the antibody is accordingly modified (altered) to improve upon, e.g., antibody/antigen binding by modifying at least one amino acid residue to decrease the binding free energy between the antibody and antigen when bound in a solvent. (justia.com)
The CDRs of V H and V L are held in close proximity by the FRs, and amino acid residues within the CDRs bind the antigen. (justia.com)
This detection antibody binds to the antigen thus completing the sandwich. (biosensis.com)
The second step is somatic hypermutation (SHM) and involves the introduction of point mutations in the V regions (antigen-binding sites) of the Ig genes, resulting in an expansion of the antibody repertoire to generate high-affinity antigen-specific antibodies. (medscape.com)
Stimulation of TCRs upon antigen binding triggers downstream signalling pathways which enables various physiological functions of the T cells. (hindawi.com)
Soluble4
Le CD154 trimérique non covalent sous sa forme soluble ou membranaire se lie au CD40 normalement monomérique et induit son oligomérisation ainsi que son homodimérisation. (umontreal.ca)
The trimeric soluble or membrane CD154 binds to the monomeric CD40 and induces its oligomerization as well as its homodimerization. (umontreal.ca)
Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. (bdbiosciences.com)
Treatment of primary hepatocyte cultures and hepatocyte-derived cell lines with soluble CD154 increased XBP1 mRNA splicing in cells subjected to either oleic acid (OA) or TM treatment. (cxcr-signal.com)
Interaction2
The interaction of CD40/CD154 plays a key role in the regulation of the immune response. (umontreal.ca)
The MR1 antibody is reported to block interaction of CD154 with CD40. (cytekbio.com)
Expressed on activated T cells1
The MR1 antibody is specific for mouse CD154, a 39 kD glycoprotein that is primarily expressed on activated T cells. (cytekbio.com)
Receptors3
It is well known that CD154 binds to three other receptors belonging to the family of integrins: αIIbβ3, α5β1, and αMβ2 (Mac-1). (umontreal.ca)
One class of non-self molecules are called antigens (short for anti body gen erators) and are defined as substances that bind to specific immune receptors and elicit an immune response. (wikidoc.org)
ATCC CRL 1476) exhibited low levels of beta-adrenergic receptors as determined by specific binding of [125I]cyanopindolol ([125I]CYP) and marginal stimulation of adenylate cyclase in plasma membranes by (-)isoproterenol. (shengsci.com)
Cognate1
Signals generated via cognate interactions between ≥ ≥ ≥ CD154-CD40 on activated T and B cells are required for T-dependent B cell responses. (cytekbio.com)
CD154 is an accessory molecule expressed predominantly on activated CD4 + lymphocytes that bind CD40. (biolegend.com)
CD154 expression on activated and resting T lymphocytes. (bdbiosciences.com)
Apoptosis1
To enhance PD-1 inhibitor nivolumab efficacy, we co-administered a CSF-1R inhibitor BLZ945 to ablate CD163+ M2-TAMs and strengthened CD154+CD8+ T-cell functionality and GBM apoptosis on-chip. (elifesciences.org)
Secretion1
Moreover, CD154 reduced the inhibition of apoB100 secretion by HepG2 cells grown in the presence of high concentrations of OA, an effect suppressed by XBP1 mRNA silencing and in HepG2 cells expressing a dominant negative form of inositol requiring ER-to-nucleus signaling protein-1. (cxcr-signal.com)
Macrophages3
Here, we report that the mutant CD154 129E → V was superior to the wild-type CD154 in killing of Leishmania donovani, induction of inducible nitric oxide synthase (iNOS) and production of IL-12 and relative phosphorylation of p38MAPK and ERK-1/2 in PBMC-derived macrophages. (sciencecentral.in)
The real-time analysis in GBM-on-a-Chip showed that mesenchymal GBM niche attracted low number of allogeneic CD154+CD8+ T-cells but abundant CD163+ tumor-associated macrophages (TAMs), and expressed elevated PD-1/PD-L1 immune checkpoints and TGF-β1, IL-10, and CSF-1 cytokines compared to proneural GBM. (elifesciences.org)
Additionally, the hexon component of AdV capsid can bind to coagulation factor X (FX) to activate TLR4 on the surface of splenic macrophages and thereby stimulate NF-κB dependent activation of IL-1β, which may help recruit polymorphonuclear leukocytes to the marginal zone of the spleen and clear virus from the spleen rapidly [49, 50]. (genemedi.com)
MRNA2
This phenotype correlated with an altered UPR as assessed by reduced X-Box binding protein-1 (XBP1) messenger 上海皓元 RNA (mRNA) splicing and reduced phosphorylation of eukaryotic initiation factor 2α. (cxcr-signal.com)
Padariya M , Kalathiya U , The Binding Specificity of PAB1 with Poly(A) mRNA, Regulated by Its Structural Foldin g. (edu.pl)
Antibodies5
Accordingly, there remains a need for antibodies that do not provoke a strong immune response but yet bind strongly to their antigens and methods for identifying such antibodies. (justia.com)
These three crystals structures 1) identify the conformational CD40 epitope for ABBV-323 recognition 2) illustrate conformational changes which occur in the CDRs of ABBV-323 Fab upon CD40 binding and 3) develop a structural hypothesis for an agonist/antagonist switch in the LCDR1 of this proprietary class of CD40 antibodies. (biomedcentral.com)
Disrupting the binding of CD47 to SIRPα has emerged as a promising immunotherapeutic strategy for advanced cancers by potentiating antibody-dependent cellular phagocytosis (ADCP) of targeted antibodies. (aacrjournals.org)
The so-called Fluorescence Quenching Immunoassay uses two antibodies: one analyte specific antibody and one fluorophore specific antibody, whereby binding of the fluorophore specific antibody to the fluorophore reduces the fluorescence intensity. (trea.com)
Both antibodies compete in binding the fluorescently labeled analyte. (trea.com)
Triggers1
Breakdown of immune tolerance is believed to be one of the major mechanisms which triggers the production of autoantibodies by B cells and antibody forming cells, leading to inflammation upon binding to autoantigens and consequent tissue damage [ 2 ]. (hindawi.com)
Protein5
Teneliximab is a chimeric monoclonal antibody binding to the immune stimulatory protein CD40. (wikipedia.org)
The expression of class II MHC genes is regulated by a non-DNA binding protein, class II transactivator (CIITA) the """"""""master control factor"""""""" for class II MHC transcription. (grantome.com)
Hexon and penton structures form the capsid of AdV, and fiber protein mediates the binding of the virion to the cell surface and is a major determinant of viral tropism. (genemedi.com)
C-reactive protein,mannose-binding lectin, and serum amyloid P component activate complement and act as opsonins. (msdmanuals.com)
Following our observation that erythrosine B (FD&C Red No. 3) is a relatively potent inhibitor of the TNF-R-TNFα and CD40-CD154 protein-protein interactions, we investigated whether this inhibitory activity extends to any other protein-protein interactions (PPI) as well as whether any other approved food colors posses. (shengsci.com)
Peripheral1
For the detection of mouse CD154 on activated peripheral T cells, it is strongly recommended that T cells be purified before activation. (bdbiosciences.com)
Cells8
BALB/c spleen T cells, purified on a T Cell Enrichment Column (R&D Systems), were cultured for 8 hours in the presence (Panels A and C) or absence (Panel B) of plate-bound 500A2 antibody (anti-CD3e, Cat. (bdbiosciences.com)
Mouse CD154 is transiently expressed on the surfaces of activated normal T cells and certain T cell clones with a maximal level detected 6-8 hours post-activation. (bdbiosciences.com)
No. 553238) is sufficient to induce CD154 expression on CD4+ cells. (bdbiosciences.com)
It has been reported that CD8+ cells express CD154 only in response to PMA/Ionomycin treatment. (bdbiosciences.com)
Therefore, for detection of CD154, it is crucial to utilize the proper activation stimuli and to stain cells at the optimal time for CD154 expression. (bdbiosciences.com)
After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer. (absoluteantibody.com)
Binding of cross linked BE-1 will partially activate B cells. (ancell.com)
Typically, CARs consist of a target binding domain, which is an extracellular domain that is specifically expressed by CAR-T cells, a transmembrane domain, and a signalling domain, which is an intracellular domain that provides an activation signal to T cells. (nature.com)
BALB1
To this end, Balb/c mice, wild-type or deficient in CD154 (CD154KO), were fed a diet rich in olive oil. (cxcr-signal.com)
Besides binding to coagulation proteins, AdV in blood vessel can also bind to component C3 and natural [51-55]. (genemedi.com)
Costimulation1
CD154 plays an important role in T-B cell costimulation and Ig class switching. (biolegend.com)
Wild-type1
Other mutants (142E → V, 143K → A, 145Y → F) mimicked the wild-type CD154. (sciencecentral.in)
Role1
Herein, we studied the role of CD154, a key mediator of inflammation, in hepatic steatosis. (cxcr-signal.com)
Complex2
Molecular dynamics simulation suggested that these mutations induced differential conformational changes in the CD40-CD154 complex. (sciencecentral.in)
After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. (biosensis.com)
Cell1
In vitro, the effect of CD154 was studied on primary hepatocyte cultures and hepatocyte-derived cell lines. (cxcr-signal.com)
Treatment1
The presentations titled, " Long-term rejection-free renal allograft survival with Fc-modified anti-CD154 antibody monotherapy in nonhuman primates," and " Monotherapy with TNX-1500, a Fc-modified anti-CD154mAb, prolongs cardiac allograft survival in cynomolgus monkeys ," include data demonstrating that TNX-1500 treatment showed activity in preventing organ rejection and was well tolerated in non-human primates. (tonixpharma.com)
High1
The reason for this is not to be seen in the usual lovelorn romantic plot, but rather in the fact that Jozal was a rigid attempt to write an autobiographical opera that testified of the composer's own high moral values and his understanding that he was bound to fail in achieving it. (401nederlandseoperas.nl)
Free1
A 1912 score combining Wagner with Alban Berg and free atonality is bound to fascinate, at least music wise. (401nederlandseoperas.nl)